Rroxscaffold_6G00412630

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002940
Physical Location & Seq
Reverse (-)
35133099 .. 35135048
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412630.1

Sequence Viewer

Length: 1629 bp
ATGGATGCTGACTGTGGAGCTGAATTTGGTGAATGGATGGTTTTTGATGCCAAGGATAGTTGTAAGAATGTTGAAAGTTCGTTAATTTCGGATAAAGATAGAGTAAAAGATGAGACTTTGAGGGAAAGGATGGAAGGTAAAGTTGTGAGTGCTAGGGGAGTGGAATTTACCAATAACAATGAGTCTTTAGATTATCAAGACTTCAATGCTAGCAGCTGTTTTAAGATGGTAAATTGTGATGGAGTTGAAAATGAGGCTATGGAAGTTAATGTGGAAGAAGAGAGTAATCGAAATGAATTGACGCCGATGAGGGTCTCTGTGAGTACTGAATGCGAGGTTGATTTGCTCAGTGTTGCTCATTTTATCCGGTATTCGAGAATGGATGGGAATGTGTCAAGTGGTGTCAATGGAAAATCTTGTTCTTCCAAGCTAGAATTTGAAGTTTCTGATCTAGTATGGGGCAAAGTAAGGACTCATCCCTGGTGGCCCGGGCAGATTTGTGATCCTTCGGCTGCGTCAGAACTGGCAAAGAAGTGTTTTAAGAGGGGTAGTTATCTGATAGCTTACTTTGGGGATCAGACATTTGCTTGGAATGAAGCACCAAAGATTAAGCCCTTTCTGGAGCACTGCTCAAAAATGGAGAGGCAGAGTGATATGGAAGAATTCCAAAACGCAGTTGGTTGTGCGTTGGATGAAGTGTCGAGAAGGGTAGAGTTTGGGCTGGCCTGTTCTTGCATATCCAAACGAGACTATGCAAAGCTCAAAACTCAGAAGATTATTAATGCTGGAATCAGGAAAGAAGCAGCAAATAGAAGAGATGGCGGTGACAGTTCTCTTAGTGCCGCTTTTTTTGAACCTGCAAAAGTCATTAAGTTTATAAAAAAGTTGGCTCAGTTGCCATATGGCAGAGCTGACAGACTAGAACTTGTAACATCTCTAGCCCAGCTGTCAGCTTTCTGGCGTTGGAAGAGTTGTGGGCCTCTGCCAGAGGAGCATGATACTGATAATTTCCTATTGGGAGAGAAGAACCACTGCAATGAAGTAAGTGATGAAGCACTCTCAGTAGTTGAGATCGAGAAGTTGGTGCAAAAATCAGAAAGTGAAGATAACTCATTGCGTGATTGCAAGGACATATCAGATTCCAAGGCTCAGAAGTGTTTGTTTCCAATGAGTGAGAATGAATCAGAAGGGAAAGCTGGCAGTGAATTCATTTTGCAACCTTGCAACAAAAGAAAGGGTCTTGATGCTATAGCTGATGAAGATGTGCTCCCAGTAATTAAGGATGGCAACTTGGTGTGGAAATTAGAAACTAAAGATATCTCATTGCACAAGTATCGGCACATATCTGGAGATGGTATGAATCTGAGTGAAGAAGAACAAAGCTTGTCAGATTTGGAGGGTATGAAGTGTTTGTCTACTGCAATGAGTGAGAATGGATTAGAAGAGAAAGGTGATGGTAAATTGATTTCACAACCTTCTGTTAAGAAACGAAAGGAAGTTGATGCTATGGCTGATGATGCTGCTGTGAAACATAGGAAAAGTGATTCATCACAAGGGATTGGTAGCAGTGCAAACTATCAATCGTTTGGAGTTGAAGAGTTTTTTAACCTGTTTTTGCAGCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

542

Amino Acids

60.58

Weight (kDa)

4.98

Isoelectric Point (pI)

45.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 150 - 232 2.6e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 878
Acc36I ACCTGC 1 cut(s) 865
AccI GTMKAC 1 cut(s) 1415
AciI CCGC 2 cut(s) 822, 843
AclWI GGATC 2 cut(s) 497, 582
AcsI RAATTY 5 cut(s) 23, 164, 434, 662, 1205
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 325
AgsI TTSAA 6 cut(s) 74, 205, 248, 440, 854, 1595
AjnI CCWGG 1 cut(s) 479
Alw21I GWGCWC 2 cut(s) 627, 1269
Alw26I GTCTC 3 cut(s) 107, 319, 741
AlwI GGATC 2 cut(s) 497, 582
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 3 cut(s) 485, 723, 977
ApeKI GCWGC 5 cut(s) 213, 512, 803, 1520, 1618
ApoI RAATTY 5 cut(s) 23, 164, 434, 662, 1205
AseI ATTAAT 1 cut(s) 780
AspS9I GGNCC 2 cut(s) 486, 977
AsuC2I CCSGG 2 cut(s) 489, 490
AsuHPI GGTGA 3 cut(s) 41, 836, 1463
AsuNHI GCTAGC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 488
Bbv12I GWGCWC 2 cut(s) 627, 1269
BbvI GCAGC 4 cut(s) 225, 499, 815, 1507
BccI CCATC 9 cut(s) 31, 124, 220, 233, 377, 812, 1277, 1346, 1448
BcgI CGANNNNNNTGC 2 cut(s) 1316, 1350
BciT130I CCWGG 1 cut(s) 481
BcnI CCSGG 2 cut(s) 489, 490
BcoDI GTCTC 3 cut(s) 107, 319, 741
BfaI CTAG 6 cut(s) 153, 210, 431, 452, 920, 938
BfmI CTRYAG 1 cut(s) 1248
BfuAI ACCTGC 1 cut(s) 865
BisI GCNGC 6 cut(s) 214, 513, 804, 843, 1521, 1619
BlsI GCNGC 6 cut(s) 215, 514, 805, 844, 1522, 1620
BmcAI AGTACT 1 cut(s) 325
Bme1390I CCNGG 3 cut(s) 481, 489, 490
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 2 cut(s) 486, 977
BmrFI CCNGG 3 cut(s) 481, 489, 490
BmrI ACTGGG 1 cut(s) 1265
BmsI GCATC 4 cut(s) 37, 1234, 1492, 1507
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 1265
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 2 cut(s) 641, 1368
BpuMI CCSGG 2 cut(s) 489, 490
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 4 cut(s) 51, 479, 488, 1143
BsaWI WCCGGW 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 528, 1271
Bse3DI GCAATG 4 cut(s) 1042, 1112, 1322, 1428
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 4 cut(s) 51, 479, 488, 1143
BseGI GGATG 7 cut(s) 10, 42, 135, 388, 475, 697, 1288
BseMI GCAATG 4 cut(s) 1042, 1112, 1322, 1428
BseMII CTCAG 6 cut(s) 361, 782, 905, 1074, 1163, 1355
BseNI ACTGG 2 cut(s) 528, 1271
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 4 cut(s) 225, 499, 815, 1507
BseYI CCCAGC 1 cut(s) 942
BshFI GGCC 3 cut(s) 487, 725, 979
BsiHKAI GWGCWC 2 cut(s) 627, 1269
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 2 cut(s) 367, 489
BsmAI GTCTC 3 cut(s) 107, 319, 741
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 3 cut(s) 487, 725, 979
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 627, 1269
Bsp143I GATC 4 cut(s) 448, 502, 574, 1071
BspACI CCGC 2 cut(s) 822, 843
BspANI GGCC 3 cut(s) 487, 725, 979
BspCNI CTCAG 6 cut(s) 360, 781, 904, 1073, 1162, 1356
BspMI ACCTGC 1 cut(s) 865
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 2 cut(s) 497, 582
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 4 cut(s) 1042, 1112, 1322, 1428
BsrI ACTGG 2 cut(s) 528, 1271
BssECI CCNNGG 4 cut(s) 51, 479, 488, 1143
BssMI GATC 4 cut(s) 448, 502, 574, 1071
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 51, 1143
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 2 cut(s) 14, 830
Bst6I CTCTTC 5 cut(s) 273, 808, 962, 1437, 1590
BstACI GRCGYC 1 cut(s) 302
BstC8I GCNNGC 3 cut(s) 211, 723, 1198
BstDEI CTNAG 7 cut(s) 347, 768, 836, 891, 1060, 1149, 1364
BstF5I GGATG 7 cut(s) 10, 42, 135, 388, 475, 697, 1288
BstKTI GATC 4 cut(s) 451, 505, 577, 1074
BstMAI GTCTC 3 cut(s) 107, 319, 741
BstMBI GATC 4 cut(s) 448, 502, 574, 1071
BstMWI GCNNNNNNNGC 2 cut(s) 991, 1517
BstNI CCWGG 1 cut(s) 481
BstSCI CCNGG 3 cut(s) 479, 487, 488
BstSFI CTRYAG 1 cut(s) 1248
BstV1I GCAGC 4 cut(s) 225, 499, 815, 1507
BsuRI GGCC 3 cut(s) 487, 725, 979
BtsCI GGATG 7 cut(s) 10, 42, 135, 388, 475, 697, 1288
BtsI GCAGTG 4 cut(s) 625, 1030, 1207, 1573
BtsIMutI CAGTG 5 cut(s) 355, 625, 1030, 1207, 1573
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 3 cut(s) 211, 723, 1198
Cfr13I GGNCC 2 cut(s) 486, 977
Cfr9I CCCGGG 1 cut(s) 488
CseI GACGC 2 cut(s) 310, 504
Csp6I GTAC 1 cut(s) 324
CviAII CATG 1 cut(s) 995
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 7 cut(s) 347, 768, 836, 891, 1060, 1149, 1364
DpnI GATC 4 cut(s) 450, 504, 576, 1073
DpnII GATC 4 cut(s) 448, 502, 574, 1071
Eam1104I CTCTTC 5 cut(s) 273, 808, 962, 1437, 1590
EarI CTCTTC 5 cut(s) 273, 808, 962, 1437, 1590
Eco130I CCWWGG 2 cut(s) 51, 1143
Eco31I GGTCTC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 1318
Eco88I CYCGRG 1 cut(s) 488
EcoRI GAATTC 2 cut(s) 662, 1205
EcoRII CCWGG 1 cut(s) 479
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 2 cut(s) 51, 1143
ErhI CCWWGG 2 cut(s) 51, 1143
FaeI CATG 1 cut(s) 998
FatI CATG 1 cut(s) 994
FauNDI CATATG 1 cut(s) 901
FblI GTMKAC 1 cut(s) 1415
Fnu4HI GCNGC 6 cut(s) 214, 513, 804, 843, 1521, 1619
FokI GGATG 7 cut(s) 17, 49, 142, 395, 462, 704, 1295
Fsp4HI GCNGC 6 cut(s) 214, 513, 804, 843, 1521, 1619
FspBI CTAG 6 cut(s) 153, 210, 431, 452, 920, 938
GluI GCNGC 6 cut(s) 214, 513, 804, 843, 1521, 1619
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 2 cut(s) 641, 1368
HaeIII GGCC 3 cut(s) 487, 725, 979
HapII CCGG 2 cut(s) 367, 489
HgaI GACGC 2 cut(s) 310, 504
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 1 cut(s) 998
HindIII AAGCTT 1 cut(s) 1381
HinfI GANTC 7 cut(s) 182, 472, 789, 1139, 1181, 1360, 1544
HpaII CCGG 2 cut(s) 367, 489
HphI GGTGA 3 cut(s) 41, 836, 1463
Hpy166II GTNNAC 1 cut(s) 1416
Hpy188III TCNNGA 8 cut(s) 197, 375, 620, 702, 793, 1075, 1241, 1347
Hpy8I GTNNAC 1 cut(s) 1416
HpyAV CCTTC 5 cut(s) 128, 516, 699, 1181, 1485
HpyCH4III ACNGT 2 cut(s) 14, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 991, 1517
HpyF3I CTNAG 7 cut(s) 347, 768, 836, 891, 1060, 1149, 1364
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 1 cut(s) 998
Kzo9I GATC 4 cut(s) 448, 502, 574, 1071
LmnI GCTCC 4 cut(s) 17, 622, 991, 1272
Lsp1109I GCAGC 4 cut(s) 225, 499, 815, 1507
LweI GCATC 4 cut(s) 37, 1234, 1492, 1507
MaeI CTAG 6 cut(s) 153, 210, 431, 452, 920, 938
MaeIII GTNAC 2 cut(s) 824, 928
MalI GATC 4 cut(s) 450, 504, 576, 1073
MboI GATC 4 cut(s) 448, 502, 574, 1071
MhlI GDGCHC 2 cut(s) 627, 1269
MlyI GAGTC 2 cut(s) 191, 466
MmeI TCCRAC 2 cut(s) 669, 944
MnlI CCTC 9 cut(s) 114, 247, 303, 328, 537, 636, 982, 990, 1390
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 2 cut(s) 216, 946
MspI CCGG 2 cut(s) 367, 489
MspR9I CCNGG 3 cut(s) 481, 489, 490
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 1 cut(s) 481
MwoI GCNNNNNNNGC 2 cut(s) 991, 1517
NciI CCSGG 2 cut(s) 489, 490
NdeI CATATG 1 cut(s) 901
NdeII GATC 4 cut(s) 448, 502, 574, 1071
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 1 cut(s) 998
NmuCI GTSAC 1 cut(s) 824
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 5 cut(s) 789, 1139, 1181, 1360, 1544
PkrI GCNGC 6 cut(s) 215, 514, 805, 844, 1522, 1620
PleI GAGTC 2 cut(s) 190, 466
PpsI GAGTC 2 cut(s) 190, 466
PshBI ATTAAT 1 cut(s) 780
PsiI TTATAA 1 cut(s) 878
Psp6I CCWGG 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 1 cut(s) 479
PspPI GGNCC 2 cut(s) 486, 977
PvuII CAGCTG 2 cut(s) 216, 946
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
RseI CAYNNNNRTG 1 cut(s) 1035
SatI GCNGC 6 cut(s) 214, 513, 804, 843, 1521, 1619
Sau3AI GATC 4 cut(s) 448, 502, 574, 1071
Sau96I GGNCC 2 cut(s) 486, 977
ScaI AGTACT 1 cut(s) 325
SchI GAGTC 2 cut(s) 191, 466
ScrFI CCNGG 3 cut(s) 481, 489, 490
SduI GDGCHC 2 cut(s) 627, 1269
SfaNI GCATC 4 cut(s) 37, 1234, 1492, 1507
SfcI CTRYAG 1 cut(s) 1248
SmaI CCCGGG 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 1035
SrfI GCCCGGGC 1 cut(s) 490
SsiI CCGC 2 cut(s) 822, 843
SspMI CTAG 6 cut(s) 153, 210, 431, 452, 920, 938
StyD4I CCNGG 3 cut(s) 479, 487, 488
StyI CCWWGG 2 cut(s) 51, 1143
TaaI ACNGT 2 cut(s) 14, 830
TaqI TCGA 4 cut(s) 289, 374, 701, 1074
TatI WGTACW 1 cut(s) 323
TauI GCSGC 1 cut(s) 845
TfiI GAWTC 5 cut(s) 789, 1139, 1181, 1360, 1544
TscAI CASTG 5 cut(s) 355, 632, 1037, 1207, 1573
TseFI GTSAC 1 cut(s) 824
TseI GCWGC 5 cut(s) 213, 512, 803, 1520, 1618
Tsp45I GTSAC 1 cut(s) 824
TspMI CCCGGG 1 cut(s) 488
TspRI CASTG 5 cut(s) 355, 632, 1037, 1207, 1573
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 5 cut(s) 23, 164, 434, 662, 1205
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 1415
XspI CTAG 6 cut(s) 153, 210, 431, 452, 920, 938
ZrmI AGTACT 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.