Rroxscaffold_6G00412560

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002940
Physical Location & Seq
Reverse (-)
35105303 .. 35109420
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412560.1

Sequence Viewer

Length: 3384 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGAGCGTTGATGATAGTGATGCCGTAGTGGGTGAGGAGATGAGTGAGGGTTTGGAAGAGGGAGATGAATTGGTAGATGGAGGTGGTGATTCTGGAGGTAGAGTAGGACCTGTAGATATTGAGATTGATGAGAAAGGTGGAGATGGAGTGACAGAGGGTTTGGAGGGGACTCAATTTGGGGGTACTGATGTGAATGCAGCAAGTGAGAAAGGGTCGGTTTCGGAGTCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAGTCTTTAGTGACAAGGATAGGGGCAAAGATGAGATTTTGGGGGAATGCGTGGAAGCTGAAGTTGTGGGTGCTGTGGAAGGGAATCGTTCAGTGAGTTTCGAGGCGAAGGAGGATGGAACAGTTGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCACAAGTAGATGAGGAAGAGAGAGAAAGAGAGCAGAATGTGGAGCGTAGCCAGGTTGAAGAGAAGAGTGCCCATGCTACAAGTTCTAACGTGGAGCATGAGGCTACAGGGTCTGTTGCAGAAATACCGCAGGACATCGCAGCTGTGGAAGTGAAAATTGCTGGTATCGATGAATCTGGGCATGATGGAGACCTGAATGCAAGCACTGGTTCCCAGATGGCTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGGAGTAAAGACCGGTGAAGATGTGTTGATGTCTGAATATGCTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGACTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCGGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTTTGGCTTGGCCTGTTCTTGCATTTCCAAAGAAGTTTATGCAAAGCTCAAAACACAGACGATCATTAATGCTGGAATCAAGGAAGAAGCATGTAGAAGAGATGGTGGTGATAGTTCACTGGGTGCTGCTTCTTTTGAACCCTTGAAACTCATTAACTTTGCAAAACAAGCCGCCAAGCTGCCATTTGCCAGAGCTGACAGACTCGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGGTTGCTGGAAAATGATGCCGACATTTCACTGTTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGTTGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCAGAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGATAGTTCCCAACCAGGTAAAAAAGGGAAAAACTCCTCAAATGTAACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTGAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCGAGTAAGAAACGGAAGGCAGTTGATGGAGCTATGCCTGATGACTCTGCGGTGAAACATAGTAAAAGCGCTTCATCAGCAGTGGAAGATATTGTTTCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTACTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAAATCTTCATCGCAGAAACCACAAACTGAAAAGCTGATTGACATGGAGGATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTGCTTGGCTGCCATGAATCCCAACAAAGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCAGTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTGGCAAGCGCAGTAAGAAATCAACCAAGACTGGAAAGAAATCAATCAAACCACGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTCTTATTCTTCTGATAAGATAGTTAAGAGTCAAAATGAAAATGGAGAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTGTGGAGTCGCAGTCAAGTTCTGATTCGGAAGCACTGAAGCCAGTTCTTGACAATAAAAATGGCGAGTTGGTGTGTGGAACTGCAACAAGCGATAAGGATACTTCTACTGTTGAACTGCAAGCAAGTCTTGAGTTAAATCCCACTTTGGATTCTGAACAGAACATTGTTTGTGGAGATATTGAATCAAAAGCAGTGAACACAGCTGCTACAAGCGATAAGGATACTTCTACTGTTGAACTGCAAGCAACTCTCGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGTTTATGGAGATATTGATTCAAAAGCAGTGAACCCAGCTCTGGATGATGAAAATGGAGCATTAGTACGTGAGCCCATCTCTGTTGCCGAGTCACCAGCAGATCCCAAGTTAAGTCCCAATTTGGATTTTGAACAGGAAAATACAGTAAGAGATACTGGTGCGGAAGCAGTGAAGCCAGCTCTTGACGATGAAAGTGGAGAGTTGGTGGCTGGAGCTCTAAGTAATAAGGATGCTTCCGCTGTGGATTCGCAAGCAAGTCTTGAGTACAATCCCAATATGGATTCTGCACTGAAATTTCCTGATGGAGATCATGATTCAGAAGCAGTGAAGCCAGCTTCCCCAAGTGAGAGTTATGAGGAAGATTCTCCCGCAGCTCTGATCTTGAACTTTTCAGACTTGGAGTCTCTTCCCTCGGAAGCAAGTCTAAACAATATTTTTAGCATCTTCGGGCCTCTGAATGAATCGCAAACTGAATTGTTGAAGAAGACAAACCGGGCCAAAGTGGTTTTCAGGCGGCGTTCTGATGCTCAAAAAGCTTTCAGCAGTGCCGGGAAATACAGTACCTTTGGACCTTCACTTGAAAGTTATCGCCTCAAGTATCCGCTATCACCAAGTAAAGCTTCTCCTTCTCCGAAGGTGGGAAAGCAAGGAAAAATGGATGCATCTGCAGAAGGTGATGGAGCTAGAAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1127

Amino Acids

121.38

Weight (kDa)

4.67

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 311 - 393 2.1e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1975
Acc36I ACCTGC 1 cut(s) 1975
AccI GTMKAC 1 cut(s) 1709
AciI CCGC 8 cut(s) 605, 1368, 1820, 2849, 2925, 3057, 3202, 3290
AclWI GGATC 6 cut(s) 980, 1738, 2045, 2058, 2202, 2783
AcsI RAATTY 4 cut(s) 917, 1145, 2150, 2981
AcuI CTGAAG 4 cut(s) 387, 975, 1998, 2456
AdeI CACNNNGTG 1 cut(s) 1319
AfaI GTAC 6 cut(s) 235, 1063, 2114, 2754, 2954, 3250
AfeI AGCGCT 1 cut(s) 1840
AfiI CCNNNNNNNGG 6 cut(s) 793, 860, 2108, 2109, 2728, 3326
AflIII ACRYGT 1 cut(s) 2393
AgeI ACCGGT 1 cut(s) 752
AhdI GACNNNNNGTC 1 cut(s) 3088
AjnI CCWGG 4 cut(s) 528, 970, 1657, 2169
AjuI GAANNNNNNNTTGG 4 cut(s) 3179, 3211, 3292, 3324
Alw21I GWGCWC 2 cut(s) 1171, 2905
Alw26I GTCTC 4 cut(s) 660, 1186, 2079, 3096
AlwI GGATC 6 cut(s) 980, 1738, 2045, 2058, 2202, 2783
AlwNI CAGNNNCTG 3 cut(s) 590, 1967, 1976
Ama87I CYCGRG 2 cut(s) 1771, 2651
Aor51HI AGCGCT 1 cut(s) 1840
AoxI GGCC 5 cut(s) 968, 1206, 1419, 3137, 3183
ApaI GGGCCC 1 cut(s) 1423
ApoI RAATTY 4 cut(s) 917, 1145, 2150, 2981
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AseI ATTAAT 1 cut(s) 1263
AsiGI ACCGGT 1 cut(s) 752
AspLEI GCGC 2 cut(s) 1841, 2232
AspS9I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3137, 3183, 3257
AsuC2I CCSGG 2 cut(s) 3182, 3238
AsuHPI GGTGA 8 cut(s) 95, 149, 767, 1316, 1834, 2772, 3288, 3374
AvaI CYCGRG 2 cut(s) 1771, 2651
AvaII GGWCC 3 cut(s) 158, 902, 3257
BaeGI GKGCMC 2 cut(s) 550, 1423
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 2382, 2415
BamHI GGATCC 1 cut(s) 2050
BanII GRGCYC 3 cut(s) 1423, 2763, 2905
BarI GAAGNNNNNNTAC 2 cut(s) 1825, 1857
BbsI GAAGAC 1 cut(s) 3179
Bbv12I GWGCWC 2 cut(s) 1171, 2905
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 4 cut(s) 530, 972, 1659, 2171
BciVI GTATCC 3 cut(s) 2491, 2614, 3297
BcnI CCSGG 2 cut(s) 3182, 3238
BcoDI GTCTC 4 cut(s) 660, 1186, 2079, 3096
BfaI CTAG 3 cut(s) 713, 935, 3372
BfmI CTRYAG 5 cut(s) 162, 582, 1909, 1968, 3354
BfoI RGCGCY 1 cut(s) 1842
BfuAI ACCTGC 1 cut(s) 1975
BfuI GTATCC 3 cut(s) 2491, 2614, 3297
Bme1390I CCNGG 6 cut(s) 530, 972, 1659, 2171, 3182, 3238
Bme18I GGWCC 3 cut(s) 158, 902, 3257
BmeRI GACNNNNNGTC 1 cut(s) 3088
BmeT110I CYCGRG 2 cut(s) 1771, 2651
BmgT120I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3137, 3183, 3257
BmiI GGNNCC 7 cut(s) 688, 852, 904, 956, 1421, 2052, 2175
BmrFI CCNGG 6 cut(s) 530, 972, 1659, 2171, 3182, 3238
BmrI ACTGGG 2 cut(s) 1325, 2175
BmuI ACTGGG 2 cut(s) 1325, 2175
BpiI GAAGAC 1 cut(s) 3179
BplI GAGNNNNNCTC 2 cut(s) 2750, 2782
BpmI CTGGAG 3 cut(s) 165, 1662, 2919
BpuEI CTTGAG 4 cut(s) 1168, 2549, 2969, 3266
BpuMI CCSGG 2 cut(s) 3182, 3238
Bsa29I ATCGAT 1 cut(s) 645
BsaAI YACGTR 1 cut(s) 2756
BsaBI GATNNNNATC 2 cut(s) 2193, 2994
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 3 cut(s) 962, 971, 3099
BsaWI WCCGGW 1 cut(s) 752
Bsc4I CCNNNNNNNGG 6 cut(s) 793, 860, 2108, 2109, 2728, 3326
Bse118I RCCGGY 1 cut(s) 752
Bse1I ACTGG 7 cut(s) 688, 1320, 1565, 2181, 2257, 2441, 2848
Bse3DI GCAATG 1 cut(s) 1616
Bse8I GATNNNNATC 2 cut(s) 2193, 2994
BseBI CCWGG 4 cut(s) 530, 972, 1659, 2171
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 3 cut(s) 962, 971, 3099
BseJI GATNNNNATC 2 cut(s) 2193, 2994
BseLI CCNNNNNNNGG 6 cut(s) 793, 860, 2108, 2109, 2728, 3326
BseMI GCAATG 1 cut(s) 1616
BseMII CTCAG 4 cut(s) 1472, 1686, 2090, 2283
BseNI ACTGG 7 cut(s) 688, 1320, 1565, 2181, 2257, 2441, 2848
BseRI GAGGAG 4 cut(s) 60, 101, 1603, 1669
BseSI GKGCMC 2 cut(s) 550, 1423
BseYI CCCAGC 2 cut(s) 787, 2722
BsgI GTGCAG 1 cut(s) 2958
BshFI GGCC 5 cut(s) 970, 1208, 1421, 3139, 3185
BshTI ACCGGT 1 cut(s) 752
BshVI ATCGAT 1 cut(s) 645
BsiHKAI GWGCWC 2 cut(s) 1171, 2905
BsiHKCI CYCGRG 2 cut(s) 1771, 2651
BsiSI CCGG 3 cut(s) 753, 3181, 3237
BslFI GGGAC 4 cut(s) 232, 888, 2223, 2787
BslI CCNNNNNNNGG 6 cut(s) 793, 860, 2108, 2109, 2728, 3326
BsmAI GTCTC 4 cut(s) 660, 1186, 2079, 3096
BsmFI GGGAC 4 cut(s) 232, 888, 2223, 2787
BsmI GAATGC 3 cut(s) 250, 359, 679
BsnI GGCC 5 cut(s) 970, 1208, 1421, 3139, 3185
Bso31I GGTCTC 1 cut(s) 660
BsoBI CYCGRG 2 cut(s) 1771, 2651
Bsp120I GGGCCC 1 cut(s) 1419
Bsp1286I GDGCHC 5 cut(s) 550, 1171, 1423, 2763, 2905
Bsp1407I TGTACA 1 cut(s) 1061
Bsp143I GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
Bsp19I CCATGG 1 cut(s) 962
BspACI CCGC 8 cut(s) 605, 1368, 1820, 2849, 2925, 3057, 3202, 3290
BspANI GGCC 5 cut(s) 970, 1208, 1421, 3139, 3185
BspCNI CTCAG 4 cut(s) 1471, 1687, 2089, 2284
BspDI ATCGAT 1 cut(s) 645
BspHI TCATGA 1 cut(s) 2998
BspLI GGNNCC 7 cut(s) 688, 852, 904, 956, 1421, 2052, 2175
BspMAI CTGCAG 2 cut(s) 1972, 3358
BspMI ACCTGC 1 cut(s) 1975
BspPI GGATC 6 cut(s) 980, 1738, 2045, 2058, 2202, 2783
BspTNI GGTCTC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 1616
BsrFI RCCGGY 1 cut(s) 752
BsrGI TGTACA 1 cut(s) 1061
BsrI ACTGG 7 cut(s) 688, 1320, 1565, 2181, 2257, 2441, 2848
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 3 cut(s) 962, 971, 3099
BssMI GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
BssT1I CCWWGG 1 cut(s) 962
Bst2UI CCWGG 4 cut(s) 530, 972, 1659, 2171
Bst4CI ACNGT 9 cut(s) 430, 855, 1034, 1518, 1751, 2509, 2632, 2833, 3248
Bst6I CTCTTC 7 cut(s) 102, 462, 489, 531, 536, 1288, 3099
BstAPI GCANNNNNTGC 1 cut(s) 1967
BstAUI TGTACA 1 cut(s) 1061
BstBAI YACGTR 1 cut(s) 2756
BstC8I GCNNGC 8 cut(s) 679, 2228, 2380, 2520, 2643, 2865, 2940, 3021
BstDEI CTNAG 6 cut(s) 291, 1458, 1695, 2076, 2292, 2906
BstDSI CCRYGG 1 cut(s) 962
BstH2I RGCGCY 1 cut(s) 1842
BstHHI GCGC 2 cut(s) 1841, 2232
BstKTI GATC 9 cut(s) 934, 988, 1260, 1733, 2053, 2197, 2791, 2998, 3069
BstMAI GTCTC 4 cut(s) 660, 1186, 2079, 3096
BstMBI GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
BstMWI GCNNNNNNNGC 6 cut(s) 788, 1364, 1847, 1967, 2077, 3221
BstNI CCWGG 4 cut(s) 530, 972, 1659, 2171
BstNSI RCATGY 3 cut(s) 1290, 2122, 2397
BstSCI CCNGG 6 cut(s) 528, 970, 1657, 2169, 3180, 3236
BstSFI CTRYAG 5 cut(s) 162, 582, 1909, 1968, 3354
BstSLI GKGCMC 2 cut(s) 550, 1423
BstV2I GAAGAC 1 cut(s) 3179
BstX2I RGATCY 2 cut(s) 2050, 2788
BstYI RGATCY 2 cut(s) 2050, 2788
Bsu15I ATCGAT 1 cut(s) 645
BsuI GTATCC 3 cut(s) 2491, 2614, 3297
BsuRI GGCC 5 cut(s) 970, 1208, 1421, 3139, 3185
BsuTUI ATCGAT 1 cut(s) 645
BtgI CCRYGG 1 cut(s) 962
BtgZI GCGATG 2 cut(s) 598, 1995
BtsI GCAGTG 6 cut(s) 1857, 2598, 2721, 2862, 3018, 3238
BveI ACCTGC 1 cut(s) 1975
Cac8I GCNNGC 8 cut(s) 679, 2228, 2380, 2520, 2643, 2865, 2940, 3021
CaiI CAGNNNCTG 3 cut(s) 590, 1967, 1976
CciI TCATGA 1 cut(s) 2998
CfoI GCGC 2 cut(s) 1841, 2232
Cfr10I RCCGGY 1 cut(s) 752
Cfr13I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3137, 3183, 3257
ClaI ATCGAT 1 cut(s) 645
CsiI ACCWGGT 1 cut(s) 1657
Csp6I GTAC 6 cut(s) 234, 1062, 2113, 2753, 2953, 3249
CspAI ACCGGT 1 cut(s) 752
CviQI GTAC 6 cut(s) 234, 1062, 2113, 2753, 2953, 3249
DdeI CTNAG 6 cut(s) 291, 1458, 1695, 2076, 2292, 2906
DpnI GATC 9 cut(s) 933, 987, 1259, 1732, 2052, 2196, 2790, 2997, 3068
DpnII GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
DraIII CACNNNGTG 1 cut(s) 1319
DriI GACNNNNNGTC 1 cut(s) 3088
Eam1104I CTCTTC 7 cut(s) 102, 462, 489, 531, 536, 1288, 3099
Eam1105I GACNNNNNGTC 1 cut(s) 3088
EarI CTCTTC 7 cut(s) 102, 462, 489, 531, 536, 1288, 3099
Ecl136II GAGCTC 1 cut(s) 2903
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 3 cut(s) 1423, 2763, 2905
Eco31I GGTCTC 1 cut(s) 660
Eco47I GGWCC 3 cut(s) 158, 902, 3257
Eco47III AGCGCT 1 cut(s) 1840
Eco53kI GAGCTC 1 cut(s) 2903
Eco57I CTGAAG 4 cut(s) 387, 975, 1998, 2456
Eco88I CYCGRG 2 cut(s) 1771, 2651
EcoICRI GAGCTC 1 cut(s) 2903
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1145
EcoRII CCWGG 4 cut(s) 528, 970, 1657, 2169
EcoT14I CCWWGG 1 cut(s) 962
EcoT22I ATGCAT 1 cut(s) 3352
EcoT38I GRGCYC 3 cut(s) 1423, 2763, 2905
ErhI CCWWGG 1 cut(s) 962
FalI AAGNNNNNCTT 8 cut(s) 1545, 1577, 2511, 2543, 2931, 2963, 3292, 3324
FaqI GGGAC 4 cut(s) 232, 888, 2223, 2787
FauI CCCGC 1 cut(s) 3064
FblI GTMKAC 1 cut(s) 1709
FriOI GRGCYC 3 cut(s) 1423, 2763, 2905
FspBI CTAG 3 cut(s) 713, 935, 3372
GlaI GCGC 2 cut(s) 1840, 2231
GsaI CCCAGC 2 cut(s) 791, 2726
GsuI CTGGAG 3 cut(s) 165, 1662, 2919
HaeII RGCGCY 1 cut(s) 1842
HaeIII GGCC 5 cut(s) 970, 1208, 1421, 3139, 3185
HapII CCGG 3 cut(s) 753, 3181, 3237
HhaI GCGC 2 cut(s) 1841, 2232
Hin6I GCGC 2 cut(s) 1839, 2230
HinP1I GCGC 2 cut(s) 1839, 2230
HindIII AAGCTT 3 cut(s) 924, 3222, 3306
HpaII CCGG 3 cut(s) 753, 3181, 3237
HphI GGTGA 8 cut(s) 95, 149, 767, 1316, 1834, 2772, 3288, 3374
Hpy166II GTNNAC 5 cut(s) 710, 1313, 1710, 2596, 2719
Hpy8I GTNNAC 5 cut(s) 710, 1313, 1710, 2596, 2719
HpyCH4III ACNGT 9 cut(s) 430, 855, 1034, 1518, 1751, 2509, 2632, 2833, 3248
HpyCH4IV ACGT 3 cut(s) 567, 725, 2755
HpyF10VI GCNNNNNNNGC 6 cut(s) 788, 1364, 1847, 1967, 2077, 3221
HpyF3I CTNAG 6 cut(s) 291, 1458, 1695, 2076, 2292, 2906
HpySE526I ACGT 3 cut(s) 567, 725, 2755
HspAI GCGC 2 cut(s) 1839, 2230
Kzo9I GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
MabI ACCWGGT 1 cut(s) 1657
MaeI CTAG 3 cut(s) 713, 935, 3372
MaeII ACGT 3 cut(s) 567, 725, 2755
MaeIII GTNAC 5 cut(s) 199, 317, 1408, 1687, 2778
MalI GATC 9 cut(s) 933, 987, 1259, 1732, 2052, 2196, 2790, 2997, 3068
MboI GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
MfeI CAATTG 1 cut(s) 2198
MflI RGATCY 2 cut(s) 2050, 2788
MhlI GDGCHC 5 cut(s) 550, 1171, 1423, 2763, 2905
MlyI GAGTC 9 cut(s) 214, 284, 1392, 1808, 2306, 2350, 2414, 2786, 3098
MmeI TCCRAC 4 cut(s) 708, 1424, 1876, 2277
Mph1103I ATGCAT 1 cut(s) 3352
MspA1I CMGCKG 4 cut(s) 620, 1462, 2603, 2927
MspI CCGG 3 cut(s) 753, 3181, 3237
MspR9I CCNGG 6 cut(s) 530, 972, 1659, 2171, 3182, 3238
MunI CAATTG 1 cut(s) 2198
Mva1269I GAATGC 3 cut(s) 250, 359, 679
MvaI CCWGG 4 cut(s) 530, 972, 1659, 2171
MwoI GCNNNNNNNGC 6 cut(s) 788, 1364, 1847, 1967, 2077, 3221
NciI CCSGG 2 cut(s) 3182, 3238
NcoI CCATGG 1 cut(s) 962
NdeII GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
NlaIV GGNNCC 7 cut(s) 688, 852, 904, 956, 1421, 2052, 2175
NmeAIII GCCGAG 1 cut(s) 2800
NmuCI GTSAC 3 cut(s) 199, 317, 2778
NsiI ATGCAT 1 cut(s) 3352
NspI RCATGY 3 cut(s) 1290, 2122, 2397
PaeR7I CTCGAG 2 cut(s) 1771, 2651
PagI TCATGA 1 cut(s) 2998
PaqCI CACCTGC 1 cut(s) 1975
PciI ACATGT 1 cut(s) 2393
PctI GAATGC 3 cut(s) 250, 359, 679
PinAI ACCGGT 1 cut(s) 752
PleI GAGTC 9 cut(s) 214, 283, 1392, 1808, 2306, 2349, 2413, 2785, 3097
PpsI GAGTC 9 cut(s) 214, 283, 1392, 1808, 2306, 2349, 2413, 2785, 3097
Ppu21I YACGTR 1 cut(s) 2756
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2393
PshBI ATTAAT 1 cut(s) 1263
Psp124BI GAGCTC 1 cut(s) 2905
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 4 cut(s) 528, 970, 1657, 2169
PspFI CCCAGC 2 cut(s) 787, 2722
PspGI CCWGG 4 cut(s) 528, 970, 1657, 2169
PspN4I GGNNCC 7 cut(s) 688, 852, 904, 956, 1421, 2052, 2175
PspOMI GGGCCC 1 cut(s) 1419
PspPI GGNCC 7 cut(s) 158, 902, 1419, 1420, 3137, 3183, 3257
PspPPI RGGWCCY 1 cut(s) 158
PspXI VCTCGAGB 1 cut(s) 1771
PstI CTGCAG 2 cut(s) 1972, 3358
PstNI CAGNNNCTG 3 cut(s) 590, 1967, 1976
PsuI RGATCY 2 cut(s) 2050, 2788
PvuII CAGCTG 3 cut(s) 620, 1462, 2603
RsaI GTAC 6 cut(s) 235, 1063, 2114, 2754, 2954, 3250
RsaNI GTAC 6 cut(s) 234, 1062, 2113, 2753, 2953, 3249
SacI GAGCTC 1 cut(s) 2905
Sau3AI GATC 9 cut(s) 931, 985, 1257, 1730, 2050, 2194, 2788, 2995, 3066
Sau96I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3137, 3183, 3257
SchI GAGTC 9 cut(s) 214, 284, 1392, 1808, 2306, 2350, 2414, 2786, 3098
ScrFI CCNGG 6 cut(s) 530, 972, 1659, 2171, 3182, 3238
SduI GDGCHC 5 cut(s) 550, 1171, 1423, 2763, 2905
SexAI ACCWGGT 1 cut(s) 1657
SfcI CTRYAG 5 cut(s) 162, 582, 1909, 1968, 3354
Sfr274I CTCGAG 2 cut(s) 1771, 2651
SinI GGWCC 3 cut(s) 158, 902, 3257
SlaI CTCGAG 2 cut(s) 1771, 2651
SmlI CTYRAG 6 cut(s) 1183, 1771, 2528, 2651, 2948, 3281
SmoI CTYRAG 6 cut(s) 1183, 1771, 2528, 2651, 2948, 3281
SsiI CCGC 8 cut(s) 605, 1368, 1820, 2849, 2925, 3057, 3202, 3290
SspI AATATT 3 cut(s) 1947, 2217, 3121
SspMI CTAG 3 cut(s) 713, 935, 3372
SstI GAGCTC 1 cut(s) 2905
StyD4I CCNGG 6 cut(s) 528, 970, 1657, 2169, 3180, 3236
StyI CCWWGG 1 cut(s) 962
TaaI ACNGT 9 cut(s) 430, 855, 1034, 1518, 1751, 2509, 2632, 2833, 3248
TaiI ACGT 3 cut(s) 570, 728, 2758
TaqI TCGA 6 cut(s) 278, 408, 645, 1401, 1772, 2652
TatI WGTACW 2 cut(s) 1061, 2952
TauI GCSGC 2 cut(s) 1370, 3205
TseFI GTSAC 3 cut(s) 199, 317, 2778
Tsp45I GTSAC 3 cut(s) 199, 317, 2778
TspGWI ACGGA 1 cut(s) 1798
VpaK11BI GGWCC 3 cut(s) 158, 902, 3257
VspI ATTAAT 1 cut(s) 1263
XapI RAATTY 4 cut(s) 917, 1145, 2150, 2981
XceI RCATGY 3 cut(s) 1290, 2122, 2397
XhoI CTCGAG 2 cut(s) 1771, 2651
XmiI GTMKAC 1 cut(s) 1709
XspI CTAG 3 cut(s) 713, 935, 3372
Zsp2I ATGCAT 1 cut(s) 3352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.