Rroxscaffold_6G00412620

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002940
Physical Location & Seq
Reverse (-)
35128452 .. 35131349
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412620.1

Sequence Viewer

Length: 1728 bp
ATGACACCCTCTGGGTCTTCATGCCCTCCCTCCTTCTCCTCAAAATTTGAGTTTCAGAGGTTTGGAGAACTCGGTCAATCAAACCGCGAAAGTCCCAAGACGTATACGGGAATGGATGGGAACATGTTTAGTGATGTGAATGGACGGTATGATCCCAAACCGGAATTCCATGTTTATGATATAGTATGGGGGAAAGTAAGGGGTTATCCCTGGTGGCCTGGACGGATATGTGATCCTTCAGCTTCATCGAATGAGGCCAAGATGCTTTTCAGAAAGGGTAGTTATTTGATAGATTATTTTGGGGATCACTCATTTACTTGGAATGAAGCACCACATATTAAGCCATTTCTGGATTATTTCTCGCAAATGGAGAAGCAGAGCAACAAGGACGAATTCCAAAATGCCATTGCTTGTGCTCTGGACGAATTCTTAAGACGGGTACAGTTGGGGGTGGTCTGTTCTTGCATATCTGGAGAGGTATATCCAAAACTCAAAAATCAGATAATTATTAGTGATGGAGTCAGGCAAGAAGAAGAAGAAGAAGACAGTGGAAATGCTGCTGACCAACTAGAACTTTCAACATCAGTGATCCAATTATCAGCTTTCTATTGTTGGAAGAGTTCTCAGCTGTTGCTGCCAACGGAGAATGATGCCTATATATCAGTATTGGGGGAGAAGACTCAATGCAATGTATTAGGTCACAATTCACTTCCAGTAACTACAGATGAGAAGCTGGTGCATAAATCTAAAAGTAAAGCTAACAATAATGATCTGGGATGTTGTGCAACATTATGGCTGATGAAAAAGCAACGCACAATTCACAACCGCAGGAATAAAATCCTGGTTCCACCCCTGTGTTTGTCTATTCCAATGACTGATAATGCAGCAGAAGGGGAAGCTAGTGGTTTGGTTGTTTTACCTTCCATGAGTGAGAATACATCAGAAGGGGAAACTAGCAGTAAATTGATTTTGCAATCTTCTGATGAGAATCCAGAGGAAGTTGATGCTATAGCGGATGAAGATGTGATGGCAGTAAGTGAAGCTGCACTTCCAGTAACAAAGGATGTGAAATTGTTGTGGAAATCAGAAGGTAAAGATAACTCATTGCACAAGTGTAATCTCATGTCTGAGTGCAGTATGAATTCGAGTGAAAAGGAGAAAATATTGTCAGATTTAAAGGCTTATAAGTGTTTGTCTACTCCAATGTGTCAGAACGGATCAGAAGGGAAAGCAGGCAGTAAATTGGCTTTGCAATCCTGTGTAAAGCGCAAGGCAGTTGATGTTATGGCTAATGACTTTTCACTGAAACATAGGAAAAGTGATTCATCAACGAGGACTAAAGATGTACTTCTGCTAACTAAGGATGAGATGTTAGTGAGGAAAACCGAAAGTAAAAGTAACTCGATGCATAAGTGCAAGCACATAAATGGAGATAAAATGAATCCAAGTAAAGAACAGAAAAGCTTATCGGATTTAAATGGTGAGAAGTGTTTGTCTCCAACAAGTGAGTATAGATTACCGTCTGATCATACATTGACTTTGCACTCCTCTAGTAGTATACAAAAGGAAGTTGATCCTATAGCTGATGACTCTGCACTGGAAGATTGGGAAAGCTATTTATCAGCAGGGGATGTTGAGCAGTCTTTTGGAGTCGCAGATTTTATTAACATGTTTCTACAGAGGGAGCACACTTCTGGAGCTGGAGAAAGGATCTGTCTGGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

575

Amino Acids

64.02

Weight (kDa)

5.33

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 60 - 142 7.4e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
AccI GTMKAC 3 cut(s) 104, 1196, 1558
AccII CGCG 1 cut(s) 87
AciI CCGC 3 cut(s) 85, 826, 1013
AclWI GGATC 7 cut(s) 146, 227, 312, 583, 1225, 1568, 1718
AcsI RAATTY 5 cut(s) 44, 164, 392, 425, 1141
AcuI CTGAAG 1 cut(s) 222
AfaI GTAC 2 cut(s) 441, 1347
AflII CTTAAG 1 cut(s) 430
AflIII ACRYGT 2 cut(s) 123, 1668
AgsI TTSAA 2 cut(s) 579, 1724
AjnI CCWGG 3 cut(s) 209, 217, 840
AjuI GAANNNNNNNTTGG 4 cut(s) 149, 181, 251, 283
AleI CACNNNNGTG 1 cut(s) 853
Alw21I GWGCWC 2 cut(s) 418, 1689
Alw26I GTCTC 1 cut(s) 1500
AlwI GGATC 7 cut(s) 146, 227, 312, 583, 1225, 1568, 1718
AoxI GGCC 2 cut(s) 215, 255
ApeKI GCWGC 4 cut(s) 557, 634, 884, 1043
ApoI RAATTY 5 cut(s) 44, 164, 392, 425, 1141
AspLEI GCGC 1 cut(s) 1269
AsuHPI GGTGA 1 cut(s) 1493
BbsI GAAGAC 3 cut(s) 9, 549, 683
Bbv12I GWGCWC 2 cut(s) 418, 1689
BbvI GCAGC 4 cut(s) 544, 621, 896, 1030
BccI CCATC 3 cut(s) 110, 509, 1021
BciT130I CCWGG 3 cut(s) 211, 219, 842
BclI TGATCA 1 cut(s) 1525
BcoDI GTCTC 1 cut(s) 1500
BfaI CTAG 4 cut(s) 569, 900, 954, 1551
BfmI CTRYAG 4 cut(s) 720, 1008, 1578, 1676
BfrI CTTAAG 1 cut(s) 430
BisI GCNGC 4 cut(s) 558, 635, 885, 1044
BlsI GCNGC 4 cut(s) 559, 636, 886, 1045
Bme1390I CCNGG 3 cut(s) 211, 219, 842
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 3 cut(s) 211, 219, 842
BmsI GCATC 4 cut(s) 252, 640, 994, 1395
BpiI GAAGAC 3 cut(s) 9, 549, 683
BpmI CTGGAG 3 cut(s) 492, 1716, 1722
BsaBI GATNNNNATC 1 cut(s) 987
BsaJI CCNNGG 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 160
Bse1I ACTGG 3 cut(s) 713, 1052, 1602
Bse3DI GCAATG 3 cut(s) 405, 694, 1103
Bse8I GATNNNNATC 1 cut(s) 987
BseBI CCWGG 3 cut(s) 211, 219, 842
BseDI CCNNGG 1 cut(s) 209
BseGI GGATG 6 cut(s) 121, 782, 1021, 1069, 1369, 1636
BseJI GATNNNNATC 1 cut(s) 987
BseMI GCAATG 3 cut(s) 405, 694, 1103
BseMII CTCAG 2 cut(s) 638, 1119
BseNI ACTGG 3 cut(s) 713, 1052, 1602
BseRI GAGGAG 2 cut(s) 28, 1537
BseXI GCAGC 4 cut(s) 544, 621, 896, 1030
BsgI GTGCAG 3 cut(s) 1029, 1153, 1578
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 2 cut(s) 217, 257
BsiHKAI GWGCWC 2 cut(s) 418, 1689
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 1500
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 2 cut(s) 217, 257
Bsp1286I GDGCHC 2 cut(s) 418, 1689
Bsp143I GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
BspACI CCGC 3 cut(s) 85, 826, 1013
BspANI GGCC 2 cut(s) 217, 257
BspCNI CTCAG 2 cut(s) 637, 1120
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 846
BspPI GGATC 7 cut(s) 146, 227, 312, 583, 1225, 1568, 1718
BspTI CTTAAG 1 cut(s) 430
BsrDI GCAATG 3 cut(s) 405, 694, 1103
BsrI ACTGG 3 cut(s) 713, 1052, 1602
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
BssNAI GTATAC 2 cut(s) 105, 1559
Bst1107I GTATAC 2 cut(s) 105, 1559
Bst2UI CCWGG 3 cut(s) 211, 219, 842
Bst4CI ACNGT 4 cut(s) 147, 444, 548, 1521
Bst6I CTCTTC 1 cut(s) 611
BstAFI CTTAAG 1 cut(s) 430
BstC8I GCNNGC 2 cut(s) 1234, 1418
BstDEI CTNAG 3 cut(s) 624, 1128, 1359
BstF5I GGATG 6 cut(s) 121, 782, 1021, 1069, 1369, 1636
BstFNI CGCG 1 cut(s) 87
BstHHI GCGC 1 cut(s) 1269
BstKTI GATC 9 cut(s) 154, 235, 307, 591, 772, 1220, 1528, 1576, 1713
BstMAI GTCTC 1 cut(s) 1500
BstMBI GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
BstMWI GCNNNNNNNGC 1 cut(s) 634
BstNI CCWGG 3 cut(s) 211, 219, 842
BstNSI RCATGY 2 cut(s) 127, 1672
BstSCI CCNGG 3 cut(s) 209, 217, 840
BstSFI CTRYAG 4 cut(s) 720, 1008, 1578, 1676
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 4 cut(s) 544, 621, 896, 1030
BstV2I GAAGAC 3 cut(s) 9, 549, 683
BstX2I RGATCY 1 cut(s) 1710
BstYI RGATCY 1 cut(s) 1710
BstZ17I GTATAC 2 cut(s) 105, 1559
BsuRI GGCC 2 cut(s) 217, 257
BtsCI GGATG 6 cut(s) 121, 782, 1021, 1069, 1369, 1636
BtsIMutI CAGTG 4 cut(s) 553, 591, 1301, 1595
Cac8I GCNNGC 2 cut(s) 1234, 1418
CfoI GCGC 1 cut(s) 1269
Csp6I GTAC 2 cut(s) 440, 1346
CviAII CATG 6 cut(s) 21, 124, 170, 925, 1123, 1669
CviQI GTAC 2 cut(s) 440, 1346
DdeI CTNAG 3 cut(s) 624, 1128, 1359
DpnI GATC 9 cut(s) 153, 234, 306, 590, 771, 1219, 1527, 1575, 1712
DpnII GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
DraI TTTAAA 2 cut(s) 1176, 1476
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco57I CTGAAG 1 cut(s) 222
EcoRI GAATTC 4 cut(s) 164, 392, 425, 1141
EcoRII CCWGG 3 cut(s) 209, 217, 840
EcoT22I ATGCAT 1 cut(s) 1410
FaeI CATG 6 cut(s) 24, 127, 173, 928, 1126, 1672
FalI AAGNNNNNCTT 4 cut(s) 1032, 1064, 1332, 1364
FaqI GGGAC 1 cut(s) 78
FatI CATG 6 cut(s) 20, 123, 169, 924, 1122, 1668
FbaI TGATCA 1 cut(s) 1525
FblI GTMKAC 3 cut(s) 104, 1196, 1558
Fnu4HI GCNGC 4 cut(s) 558, 635, 885, 1044
FokI GGATG 6 cut(s) 128, 789, 1028, 1076, 1376, 1643
Fsp4HI GCNGC 4 cut(s) 558, 635, 885, 1044
FspBI CTAG 4 cut(s) 569, 900, 954, 1551
GlaI GCGC 1 cut(s) 1268
GluI GCNGC 4 cut(s) 558, 635, 885, 1044
GsuI CTGGAG 3 cut(s) 492, 1716, 1722
HaeIII GGCC 2 cut(s) 217, 257
HapII CCGG 1 cut(s) 161
HhaI GCGC 1 cut(s) 1269
Hin1II CATG 6 cut(s) 24, 127, 173, 928, 1126, 1672
Hin6I GCGC 1 cut(s) 1267
HinP1I GCGC 1 cut(s) 1267
HindIII AAGCTT 1 cut(s) 1462
HinfI GANTC 7 cut(s) 519, 679, 988, 1322, 1441, 1589, 1650
HpaII CCGG 1 cut(s) 161
HphI GGTGA 1 cut(s) 1493
Hpy166II GTNNAC 3 cut(s) 105, 1197, 1559
Hpy188III TCNNGA 5 cut(s) 350, 419, 471, 992, 1695
Hpy8I GTNNAC 3 cut(s) 105, 1197, 1559
HpyAV CCTTC 7 cut(s) 43, 246, 884, 930, 938, 1082, 1217
HpyCH4III ACNGT 4 cut(s) 147, 444, 548, 1521
HpyCH4IV ACGT 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 634
HpyF3I CTNAG 3 cut(s) 624, 1128, 1359
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 6 cut(s) 24, 127, 173, 928, 1126, 1672
HspAI GCGC 1 cut(s) 1267
Ksp22I TGATCA 1 cut(s) 1525
Kzo9I GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
LmnI GCTCC 2 cut(s) 1684, 1697
Lsp1109I GCAGC 4 cut(s) 544, 621, 896, 1030
LweI GCATC 4 cut(s) 252, 640, 994, 1395
MaeI CTAG 4 cut(s) 569, 900, 954, 1551
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 4 cut(s) 698, 715, 1054, 1397
MalI GATC 9 cut(s) 153, 234, 306, 590, 771, 1219, 1527, 1575, 1712
MboI GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
MflI RGATCY 1 cut(s) 1710
MhlI GDGCHC 2 cut(s) 418, 1689
MlyI GAGTC 4 cut(s) 528, 673, 1583, 1659
MmeI TCCRAC 2 cut(s) 593, 1523
Mph1103I ATGCAT 1 cut(s) 1410
MseI TTAA 5 cut(s) 339, 431, 1175, 1475, 1665
MslI CAYNNNNRTG 3 cut(s) 174, 853, 1425
MspA1I CMGCKG 1 cut(s) 628
MspCI CTTAAG 1 cut(s) 430
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 3 cut(s) 211, 219, 842
MvaI CCWGG 3 cut(s) 211, 219, 842
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 634
NdeII GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
NlaIII CATG 6 cut(s) 24, 127, 173, 928, 1126, 1672
NlaIV GGNNCC 1 cut(s) 846
NmuCI GTSAC 1 cut(s) 698
NsiI ATGCAT 1 cut(s) 1410
NspI RCATGY 2 cut(s) 127, 1672
OliI CACNNNNGTG 1 cut(s) 853
PciI ACATGT 2 cut(s) 123, 1668
PfeI GAWTC 3 cut(s) 988, 1322, 1441
PkrI GCNGC 4 cut(s) 559, 636, 886, 1045
PleI GAGTC 4 cut(s) 527, 673, 1583, 1658
PpsI GAGTC 4 cut(s) 527, 673, 1583, 1658
PscI ACATGT 2 cut(s) 123, 1668
PsiI TTATAA 1 cut(s) 1185
Psp6I CCWGG 3 cut(s) 209, 217, 840
PspGI CCWGG 3 cut(s) 209, 217, 840
PspN4I GGNNCC 1 cut(s) 846
PsuI RGATCY 1 cut(s) 1710
PvuII CAGCTG 1 cut(s) 628
RsaI GTAC 2 cut(s) 441, 1347
RsaNI GTAC 2 cut(s) 440, 1346
RseI CAYNNNNRTG 3 cut(s) 174, 853, 1425
SaqAI TTAA 5 cut(s) 339, 431, 1175, 1475, 1665
SatI GCNGC 4 cut(s) 558, 635, 885, 1044
Sau3AI GATC 9 cut(s) 151, 232, 304, 588, 769, 1217, 1525, 1573, 1710
SchI GAGTC 4 cut(s) 528, 673, 1583, 1659
ScrFI CCNGG 3 cut(s) 211, 219, 842
SduI GDGCHC 2 cut(s) 418, 1689
SfaNI GCATC 4 cut(s) 252, 640, 994, 1395
SfcI CTRYAG 4 cut(s) 720, 1008, 1578, 1676
SmiI ATTTAAAT 1 cut(s) 1476
SmiMI CAYNNNNRTG 3 cut(s) 174, 853, 1425
SmlI CTYRAG 1 cut(s) 430
SmoI CTYRAG 1 cut(s) 430
SsiI CCGC 3 cut(s) 85, 826, 1013
SspI AATATT 1 cut(s) 1164
SspMI CTAG 4 cut(s) 569, 900, 954, 1551
StyD4I CCNGG 3 cut(s) 209, 217, 840
SwaI ATTTAAAT 1 cut(s) 1476
TaaI ACNGT 4 cut(s) 147, 444, 548, 1521
TaiI ACGT 1 cut(s) 104
TaqI TCGA 3 cut(s) 248, 1145, 1403
TaqII GACCGA 1 cut(s) 62
TatI WGTACW 1 cut(s) 1345
TfiI GAWTC 3 cut(s) 988, 1322, 1441
Tru1I TTAA 5 cut(s) 339, 431, 1175, 1475, 1665
Tru9I TTAA 5 cut(s) 339, 431, 1175, 1475, 1665
TscAI CASTG 4 cut(s) 553, 591, 1308, 1602
TseFI GTSAC 1 cut(s) 698
TseI GCWGC 4 cut(s) 557, 634, 884, 1043
Tsp45I GTSAC 1 cut(s) 698
TspDTI ATGAA 8 cut(s) 9, 234, 339, 815, 1032, 1154, 1314, 1454
TspGWI ACGGA 3 cut(s) 238, 656, 1230
TspRI CASTG 4 cut(s) 553, 591, 1308, 1602
Vha464I CTTAAG 1 cut(s) 430
XapI RAATTY 5 cut(s) 44, 164, 392, 425, 1141
XceI RCATGY 2 cut(s) 127, 1672
XmiI GTMKAC 3 cut(s) 104, 1196, 1558
XspI CTAG 4 cut(s) 569, 900, 954, 1551
Zsp2I ATGCAT 1 cut(s) 1410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.