RLG00000024409

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002940
Physical Location & Seq
Reverse (-)
35580114 .. 35583626
3513 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024409

Sequence Viewer

Length: 3513 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGAGCGTTGATGATAGTGATGCAGTAGTGGGTGAGGAGACGAGTGAGGGTTTGGAAGAGAGAGATGAATTGGTAGATGGAGGTGGTGATTCTGGAGGGAAAGTAGGACCTGTAGATATGGAGATTGATGAGAAAGGTGGAGATGGAGTGACAGAGGGTTTGGAGGGGACTAAATTTGGGGGTACTGATGCGAATGCAGCAAGTCAGAAAGGGTCGGTTTCGGAATCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAGTCTTTAGTGACAAGGATAGGGGTAAAGATGAGATTTTGAGGGAATGCGTGGAAGCTGAAGTTGTGGGTGCTGTGGAAGGGAATCGTTCAGTGAGTTTCGAGGCAAAGGAGGATGGAACAGTTGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCGCAGGTAGATGAGGAAGAGAGAGAAAGAGAACATAATGTGGAGTGTAGCCAGGTTGAAGAGAAGAGTGCCCATGCTACAAGTTCTAACATGGAGCATGAGGCTACAGGGTCTGTTGGAGAACTACCGCAGGACATCCCAGCTGCAGAAGTGAAAATTGTCAGTATCAATGAGTCTGGGCATGATGGAGACCTGAATGCAAGCACTGGTTCACAGATGGTTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGGAGTAAAGACCAGTGAAGATGTGTTGATGCCTGAATATGTTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGATTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCAGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTTTGGCTTGGCCTGTTCTTGCATTTCCAAAGAAGTATATGCAAGGCTCAAAACACAGACGATCATTAATGCTGGAATCAAGGAAGAAGCATGTAGAAGAGATGGTGGTGATAGTTCTCTGGGTGCTGCTTCTTTTGAGCCCTTGAAACTCATTAACTTTGCAAAACAAGCCGCCAAGTTGCCATTTGGCAGAGCTGACAGACTAGAACTTGTTACATCACGGGCCCAATTATCTTCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGGTTGCTGGAAAATGATGCCGACATTTCACTATTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGATGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCAGAAATCGAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGACAGTTCCCAACCAAGTAAAAAAGGGAAAAACTCCTCAAATGCAACTGCTGAGAAGGGTGAGGAATTGGAGCAGAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGATAGTTCCCACCCAAGTAAAAAAGGGAAAAACTCCTCATATGTAACTGCTGAGGAGGGTTTGTCTACTCCCATGAGTGAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCAAGTGAGAAACGAAAGGCGGTTGATGGAGCTATGTCTGATGACTCTGTGGTGAAACATAGTAAAAGCGCTTCATCAGCAGGGGAAGATTATGTTTCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTACTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAAATCTACATCGCAGAAACCACAAACTGAAAAGCTGATTGACATGGAGGATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTGCGTGGCCGCCATGAATCCCAATAAAGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCATTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTGGCAAGCGCAGTAAGAAATCAACCAAGACTGGAAAGAAATCAATCAAACCACGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCGAAGACTCTTATTCTTCTGATAAGATAGTAAAAAGTCAAAGTGAAAATGGAGAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTGTGGAGTCACAATCAAGTCCTGATTCGGAAGCAGTGAAGCCAGTTCTTGACAATAAAAATGGAGAGTTGGTGTGTGGAACTGCTACAAGCGATAAGGATACTTCTACTGTTGAGCTGCAAGCAAGTCTTGAGTTAAATCCCACTTTGGATTCTGAACAGAACATTGTTTGTGGAGATATTGAATCAAAAGCAGTGAGCACAGCTGCTACAAGCAATAAGGATACTTCTACTGTTGAACTGCAAGCAACTCTCGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGTTTGTGGAGATAATGATTCAAAAGCAGTGAACCCAGCTCTGGATGATGAAAATGGAGCATTAGTATGTGAGCCCATCTCTGTTGCTGAGTCATCAGCAGATCCCAAGTCAAATCCCAATTTGGATTTTGAACAGGAAAATACAGTTGGAGATACTGGTGCGGAAGCAGTGAAGCCAGCTCTTGACAATGAAAGTGGAGAGTTGGTGGCTGGAGCTCTAAGTAATAAGGATGCTTCCGCTGTGGATTCACAAGCAAGTCTTGAGTTTAATCCCAATATGGATTCTGCACTGAAATTTCCTGATGGAGATCATGATTCAGAAGCAGTGAAGCCAGCTTCCCCAAGTGAGAGTTGTGAGGAAGATTCTCCCGCAGCTCTGATCTTGAACTTTTCAGACTTGGAGTCTCTTCCCTCAGAAGCAAGTCTAAACAAAATTTTTAGCATCTTCGGGCCTCTGAACGAATCGCAAACCGAATTGTTGAAGAAGACTAACCGGGCCAAAGTGGTTTTCAGGCGGCGTTCTGATGCTCAAAAAGCTTTCAGCAGTGCTGGGAAATACAGTACCTTCGGACCTTCACTTGAAAGTTATCGCCTCAAGTATCCACTATCACCAAGTAAAGCAGCTTCTCCTTCTCCGAAGGTGGCAAAGCAAGGAAAAATGGATGCATCTGCAGAAGGTGATGGAGCTAGAAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1171

Amino Acids

126.04

Weight (kDa)

4.77

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 311 - 393 2.2e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2101
Acc16I TGCGCA 1 cut(s) 480
Acc36I ACCTGC 2 cut(s) 472, 2101
AccI GTMKAC 1 cut(s) 1835
AciI CCGC 8 cut(s) 605, 1368, 1916, 2217, 2975, 3051, 3183, 3328
AclWI GGATC 6 cut(s) 980, 1864, 2171, 2184, 2328, 2909
AcoI YGGCCR 1 cut(s) 2214
AcsI RAATTY 6 cut(s) 224, 917, 1145, 2276, 3107, 3246
AcuI CTGAAG 3 cut(s) 387, 975, 2124
AfaI GTAC 4 cut(s) 235, 1063, 2240, 3376
AfeI AGCGCT 1 cut(s) 1966
AfiI CCNNNNNNNGG 5 cut(s) 793, 860, 2234, 2235, 2854
AflIII ACRYGT 1 cut(s) 2519
AhdI GACNNNNNGTC 1 cut(s) 3214
AjnI CCWGG 3 cut(s) 528, 970, 2295
AjuI GAANNNNNNNTTGG 4 cut(s) 2943, 2975, 3305, 3337
Alw21I GWGCWC 3 cut(s) 1171, 2726, 3031
Alw26I GTCTC 6 cut(s) 83, 660, 1186, 1638, 2205, 3222
AlwI GGATC 6 cut(s) 980, 1864, 2171, 2184, 2328, 2909
AlwNI CAGNNNCTG 3 cut(s) 590, 2093, 2102
Ama87I CYCGRG 1 cut(s) 2777
Aor51HI AGCGCT 1 cut(s) 1966
AoxI GGCC 6 cut(s) 968, 1206, 1419, 2214, 3263, 3309
ApaI GGGCCC 1 cut(s) 1423
ApoI RAATTY 6 cut(s) 224, 917, 1145, 2276, 3107, 3246
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AseI ATTAAT 1 cut(s) 1263
AspLEI GCGC 3 cut(s) 481, 1967, 2358
AspS9I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3263, 3309, 3383
AsuC2I CCSGG 1 cut(s) 3308
AsuHPI GGTGA 7 cut(s) 95, 149, 1316, 1715, 1960, 3414, 3503
AvaI CYCGRG 1 cut(s) 2777
AvaII GGWCC 3 cut(s) 158, 902, 3383
BaeGI GKGCMC 2 cut(s) 550, 1423
BaeI ACNNNNGTAYC 2 cut(s) 2508, 2541
BamHI GGATCC 1 cut(s) 2176
BanII GRGCYC 4 cut(s) 1338, 1423, 2889, 3031
BarI GAAGNNNNNNTAC 4 cut(s) 1951, 1983, 3421, 3453
BbsI GAAGAC 2 cut(s) 2442, 3305
Bbv12I GWGCWC 3 cut(s) 1171, 2726, 3031
BbvCI CCTCAGC 1 cut(s) 1821
BciT130I CCWGG 3 cut(s) 530, 972, 2297
BciVI GTATCC 3 cut(s) 2617, 2740, 3423
BcnI CCSGG 1 cut(s) 3308
BcoDI GTCTC 6 cut(s) 83, 660, 1186, 1638, 2205, 3222
BfaI CTAG 4 cut(s) 713, 935, 1400, 3501
BfmI CTRYAG 6 cut(s) 162, 582, 621, 2035, 2094, 3483
BfoI RGCGCY 1 cut(s) 1968
BfuAI ACCTGC 2 cut(s) 472, 2101
BfuI GTATCC 3 cut(s) 2617, 2740, 3423
Bme1390I CCNGG 4 cut(s) 530, 972, 2297, 3308
Bme18I GGWCC 3 cut(s) 158, 902, 3383
BmeRI GACNNNNNGTC 1 cut(s) 3214
BmeT110I CYCGRG 1 cut(s) 2777
BmgT120I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3263, 3309, 3383
BmiI GGNNCC 6 cut(s) 852, 904, 956, 1421, 2178, 2301
BmrFI CCNGG 4 cut(s) 530, 972, 2297, 3308
BpiI GAAGAC 2 cut(s) 2442, 3305
BplI GAGNNNNNCTC 2 cut(s) 2876, 2908
BpmI CTGGAG 4 cut(s) 165, 1662, 1788, 3045
Bpu10I CCTNAGC 1 cut(s) 1821
BpuEI CTTGAG 5 cut(s) 1168, 1882, 2675, 3095, 3392
BpuMI CCSGG 1 cut(s) 3308
BsaBI GATNNNNATC 2 cut(s) 2319, 3120
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 2 cut(s) 962, 971
Bsc4I CCNNNNNNNGG 5 cut(s) 793, 860, 2234, 2235, 2854
Bse1I ACTGG 6 cut(s) 688, 753, 1565, 2383, 2567, 2974
Bse3DI GCAATG 2 cut(s) 1616, 1742
Bse8I GATNNNNATC 2 cut(s) 2319, 3120
BseBI CCWGG 3 cut(s) 530, 972, 2297
BseDI CCNNGG 2 cut(s) 962, 971
BseJI GATNNNNATC 2 cut(s) 2319, 3120
BseLI CCNNNNNNNGG 5 cut(s) 793, 860, 2234, 2235, 2854
BseMI GCAATG 2 cut(s) 1616, 1742
BseMII CTCAG 7 cut(s) 1472, 1686, 1812, 2216, 2409, 2892, 3240
BseNI ACTGG 6 cut(s) 688, 753, 1565, 2383, 2567, 2974
BseRI GAGGAG 7 cut(s) 60, 101, 1603, 1669, 1729, 1795, 1838
BseSI GKGCMC 2 cut(s) 550, 1423
BseYI CCCAGC 4 cut(s) 616, 787, 2848, 3362
BsgI GTGCAG 1 cut(s) 3084
BshFI GGCC 6 cut(s) 970, 1208, 1421, 2216, 3265, 3311
BsiHKAI GWGCWC 3 cut(s) 1171, 2726, 3031
BsiHKCI CYCGRG 1 cut(s) 2777
BsiSI CCGG 1 cut(s) 3307
BslFI GGGAC 3 cut(s) 232, 888, 2349
BslI CCNNNNNNNGG 5 cut(s) 793, 860, 2234, 2235, 2854
BsmAI GTCTC 6 cut(s) 83, 660, 1186, 1638, 2205, 3222
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 3 cut(s) 232, 888, 2349
BsmI GAATGC 3 cut(s) 250, 359, 679
BsnI GGCC 6 cut(s) 970, 1208, 1421, 2216, 3265, 3311
Bso31I GGTCTC 1 cut(s) 660
BsoBI CYCGRG 1 cut(s) 2777
Bsp120I GGGCCC 1 cut(s) 1419
Bsp1286I GDGCHC 7 cut(s) 550, 1171, 1338, 1423, 2726, 2889, 3031
Bsp1407I TGTACA 1 cut(s) 1061
Bsp143I GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
Bsp19I CCATGG 1 cut(s) 962
BspACI CCGC 8 cut(s) 605, 1368, 1916, 2217, 2975, 3051, 3183, 3328
BspANI GGCC 6 cut(s) 970, 1208, 1421, 2216, 3265, 3311
BspCNI CTCAG 7 cut(s) 1471, 1687, 1813, 2215, 2410, 2893, 3239
BspHI TCATGA 1 cut(s) 3124
BspLI GGNNCC 6 cut(s) 852, 904, 956, 1421, 2178, 2301
BspMAI CTGCAG 3 cut(s) 625, 2098, 3487
BspMI ACCTGC 2 cut(s) 472, 2101
BspPI GGATC 6 cut(s) 980, 1864, 2171, 2184, 2328, 2909
BspTNI GGTCTC 1 cut(s) 660
BsrDI GCAATG 2 cut(s) 1616, 1742
BsrGI TGTACA 1 cut(s) 1061
BsrI ACTGG 6 cut(s) 688, 753, 1565, 2383, 2567, 2974
BssECI CCNNGG 2 cut(s) 962, 971
BssMI GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
BssT1I CCWWGG 1 cut(s) 962
Bst2UI CCWGG 3 cut(s) 530, 972, 2297
Bst4CI ACNGT 9 cut(s) 430, 855, 1034, 1649, 1877, 2635, 2758, 2959, 3374
Bst6I CTCTTC 7 cut(s) 102, 462, 489, 531, 536, 1288, 3225
BstAPI GCANNNNNTGC 1 cut(s) 2093
BstAUI TGTACA 1 cut(s) 1061
BstC8I GCNNGC 7 cut(s) 679, 2354, 2506, 2646, 2769, 2991, 3147
BstDEI CTNAG 9 cut(s) 291, 1458, 1695, 1821, 2202, 2418, 2901, 3032, 3226
BstDSI CCRYGG 1 cut(s) 962
BstH2I RGCGCY 1 cut(s) 1968
BstHHI GCGC 3 cut(s) 481, 1967, 2358
BstKTI GATC 9 cut(s) 934, 988, 1260, 1859, 2179, 2323, 2917, 3124, 3195
BstMAI GTCTC 6 cut(s) 83, 660, 1186, 1638, 2205, 3222
BstMBI GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
BstMWI GCNNNNNNNGC 6 cut(s) 248, 1364, 1973, 2093, 2203, 3347
BstNI CCWGG 3 cut(s) 530, 972, 2297
BstNSI RCATGY 3 cut(s) 1290, 2248, 2523
BstSCI CCNGG 4 cut(s) 528, 970, 2295, 3306
BstSFI CTRYAG 6 cut(s) 162, 582, 621, 2035, 2094, 3483
BstSLI GKGCMC 2 cut(s) 550, 1423
BstV2I GAAGAC 2 cut(s) 2442, 3305
BstX2I RGATCY 2 cut(s) 2176, 2914
BstYI RGATCY 2 cut(s) 2176, 2914
BsuI GTATCC 3 cut(s) 2617, 2740, 3423
BsuRI GGCC 6 cut(s) 970, 1208, 1421, 2216, 3265, 3311
BtgI CCRYGG 1 cut(s) 962
BtgZI GCGATG 1 cut(s) 2121
BtsI GCAGTG 6 cut(s) 2565, 2724, 2847, 2988, 3144, 3364
BveI ACCTGC 2 cut(s) 472, 2101
Cac8I GCNNGC 7 cut(s) 679, 2354, 2506, 2646, 2769, 2991, 3147
CaiI CAGNNNCTG 3 cut(s) 590, 2093, 2102
CciI TCATGA 1 cut(s) 3124
CfoI GCGC 3 cut(s) 481, 1967, 2358
Cfr13I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3263, 3309, 3383
Csp6I GTAC 4 cut(s) 234, 1062, 2239, 3375
CspCI CAANNNNNGTGG 2 cut(s) 2989, 3024
CviQI GTAC 4 cut(s) 234, 1062, 2239, 3375
DdeI CTNAG 9 cut(s) 291, 1458, 1695, 1821, 2202, 2418, 2901, 3032, 3226
DpnI GATC 9 cut(s) 933, 987, 1259, 1858, 2178, 2322, 2916, 3123, 3194
DpnII GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
DriI GACNNNNNGTC 1 cut(s) 3214
EaeI YGGCCR 1 cut(s) 2214
Eam1104I CTCTTC 7 cut(s) 102, 462, 489, 531, 536, 1288, 3225
Eam1105I GACNNNNNGTC 1 cut(s) 3214
EarI CTCTTC 7 cut(s) 102, 462, 489, 531, 536, 1288, 3225
Ecl136II GAGCTC 1 cut(s) 3029
Eco130I CCWWGG 1 cut(s) 962
Eco24I GRGCYC 4 cut(s) 1338, 1423, 2889, 3031
Eco31I GGTCTC 1 cut(s) 660
Eco47I GGWCC 3 cut(s) 158, 902, 3383
Eco47III AGCGCT 1 cut(s) 1966
Eco53kI GAGCTC 1 cut(s) 3029
Eco57I CTGAAG 3 cut(s) 387, 975, 2124
Eco88I CYCGRG 1 cut(s) 2777
EcoICRI GAGCTC 1 cut(s) 3029
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1145
EcoRII CCWGG 3 cut(s) 528, 970, 2295
EcoT14I CCWWGG 1 cut(s) 962
EcoT22I ATGCAT 1 cut(s) 3481
EcoT38I GRGCYC 4 cut(s) 1338, 1423, 2889, 3031
ErhI CCWWGG 1 cut(s) 962
Esp3I CGTCTC 1 cut(s) 83
FalI AAGNNNNNCTT 6 cut(s) 1545, 1577, 2637, 2669, 3057, 3089
FaqI GGGAC 3 cut(s) 232, 888, 2349
FauI CCCGC 1 cut(s) 3190
FauNDI CATATG 1 cut(s) 1810
FblI GTMKAC 1 cut(s) 1835
FriOI GRGCYC 4 cut(s) 1338, 1423, 2889, 3031
FspBI CTAG 4 cut(s) 713, 935, 1400, 3501
FspI TGCGCA 1 cut(s) 480
GlaI GCGC 3 cut(s) 480, 1966, 2357
GsaI CCCAGC 4 cut(s) 620, 791, 2852, 3366
GsuI CTGGAG 4 cut(s) 165, 1662, 1788, 3045
HaeII RGCGCY 1 cut(s) 1968
HaeIII GGCC 6 cut(s) 970, 1208, 1421, 2216, 3265, 3311
HapII CCGG 1 cut(s) 3307
HhaI GCGC 3 cut(s) 481, 1967, 2358
Hin6I GCGC 3 cut(s) 479, 1965, 2356
HinP1I GCGC 3 cut(s) 479, 1965, 2356
HindIII AAGCTT 2 cut(s) 924, 3348
HpaII CCGG 1 cut(s) 3307
HphI GGTGA 7 cut(s) 95, 149, 1316, 1715, 1960, 3414, 3503
Hpy166II GTNNAC 4 cut(s) 689, 710, 1836, 2845
Hpy8I GTNNAC 4 cut(s) 689, 710, 1836, 2845
HpyCH4III ACNGT 9 cut(s) 430, 855, 1034, 1649, 1877, 2635, 2758, 2959, 3374
HpyCH4IV ACGT 1 cut(s) 725
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 1364, 1973, 2093, 2203, 3347
HpyF3I CTNAG 9 cut(s) 291, 1458, 1695, 1821, 2202, 2418, 2901, 3032, 3226
HpySE526I ACGT 1 cut(s) 725
HspAI GCGC 3 cut(s) 479, 1965, 2356
Kzo9I GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
MaeI CTAG 4 cut(s) 713, 935, 1400, 3501
MaeII ACGT 1 cut(s) 725
MaeIII GTNAC 5 cut(s) 199, 317, 1408, 1813, 2532
MalI GATC 9 cut(s) 933, 987, 1259, 1858, 2178, 2322, 2916, 3123, 3194
MboI GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
MfeI CAATTG 1 cut(s) 2324
MflI RGATCY 2 cut(s) 2176, 2914
MhlI GDGCHC 7 cut(s) 550, 1171, 1338, 1423, 2726, 2889, 3031
MlyI GAGTC 6 cut(s) 659, 1934, 2432, 2540, 2912, 3224
MmeI TCCRAC 6 cut(s) 574, 708, 1424, 2002, 2403, 2941
Mph1103I ATGCAT 1 cut(s) 3481
MslI CAYNNNNRTG 1 cut(s) 2878
MspA1I CMGCKG 4 cut(s) 620, 1462, 2729, 3053
MspI CCGG 1 cut(s) 3307
MspR9I CCNGG 4 cut(s) 530, 972, 2297, 3308
MunI CAATTG 1 cut(s) 2324
Mva1269I GAATGC 3 cut(s) 250, 359, 679
MvaI CCWGG 3 cut(s) 530, 972, 2297
MwoI GCNNNNNNNGC 6 cut(s) 248, 1364, 1973, 2093, 2203, 3347
NciI CCSGG 1 cut(s) 3308
NcoI CCATGG 1 cut(s) 962
NdeI CATATG 1 cut(s) 1810
NdeII GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
NlaIV GGNNCC 6 cut(s) 852, 904, 956, 1421, 2178, 2301
NmuCI GTSAC 3 cut(s) 199, 317, 2532
NsbI TGCGCA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 3481
NspI RCATGY 3 cut(s) 1290, 2248, 2523
PaeR7I CTCGAG 1 cut(s) 2777
PagI TCATGA 1 cut(s) 3124
PaqCI CACCTGC 1 cut(s) 2101
PciI ACATGT 1 cut(s) 2519
PctI GAATGC 3 cut(s) 250, 359, 679
PleI GAGTC 6 cut(s) 658, 1934, 2432, 2539, 2911, 3223
PpsI GAGTC 6 cut(s) 658, 1934, 2432, 2539, 2911, 3223
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2519
PshBI ATTAAT 1 cut(s) 1263
Psp124BI GAGCTC 1 cut(s) 3031
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 528, 970, 2295
PspFI CCCAGC 4 cut(s) 616, 787, 2848, 3362
PspGI CCWGG 3 cut(s) 528, 970, 2295
PspN4I GGNNCC 6 cut(s) 852, 904, 956, 1421, 2178, 2301
PspOMI GGGCCC 1 cut(s) 1419
PspPI GGNCC 7 cut(s) 158, 902, 1419, 1420, 3263, 3309, 3383
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 3 cut(s) 625, 2098, 3487
PstNI CAGNNNCTG 3 cut(s) 590, 2093, 2102
PsuI RGATCY 2 cut(s) 2176, 2914
PvuII CAGCTG 3 cut(s) 620, 1462, 2729
RsaI GTAC 4 cut(s) 235, 1063, 2240, 3376
RsaNI GTAC 4 cut(s) 234, 1062, 2239, 3375
RseI CAYNNNNRTG 1 cut(s) 2878
SacI GAGCTC 1 cut(s) 3031
Sau3AI GATC 9 cut(s) 931, 985, 1257, 1856, 2176, 2320, 2914, 3121, 3192
Sau96I GGNCC 7 cut(s) 158, 902, 1419, 1420, 3263, 3309, 3383
SchI GAGTC 6 cut(s) 659, 1934, 2432, 2540, 2912, 3224
ScrFI CCNGG 4 cut(s) 530, 972, 2297, 3308
SduI GDGCHC 7 cut(s) 550, 1171, 1338, 1423, 2726, 2889, 3031
SfcI CTRYAG 6 cut(s) 162, 582, 621, 2035, 2094, 3483
Sfr274I CTCGAG 1 cut(s) 2777
SinI GGWCC 3 cut(s) 158, 902, 3383
SlaI CTCGAG 1 cut(s) 2777
SmiMI CAYNNNNRTG 1 cut(s) 2878
SmlI CTYRAG 6 cut(s) 1183, 1897, 2654, 2777, 3074, 3407
SmoI CTYRAG 6 cut(s) 1183, 1897, 2654, 2777, 3074, 3407
SsiI CCGC 8 cut(s) 605, 1368, 1916, 2217, 2975, 3051, 3183, 3328
SspI AATATT 2 cut(s) 2073, 2343
SspMI CTAG 4 cut(s) 713, 935, 1400, 3501
SstI GAGCTC 1 cut(s) 3031
StyD4I CCNGG 4 cut(s) 528, 970, 2295, 3306
StyI CCWWGG 1 cut(s) 962
TaaI ACNGT 9 cut(s) 430, 855, 1034, 1649, 1877, 2635, 2758, 2959, 3374
TaiI ACGT 1 cut(s) 728
TaqI TCGA 5 cut(s) 278, 408, 1598, 2433, 2778
TatI WGTACW 1 cut(s) 1061
TauI GCSGC 3 cut(s) 1370, 2219, 3331
TseFI GTSAC 3 cut(s) 199, 317, 2532
Tsp45I GTSAC 3 cut(s) 199, 317, 2532
VpaK11BI GGWCC 3 cut(s) 158, 902, 3383
VspI ATTAAT 1 cut(s) 1263
XapI RAATTY 6 cut(s) 224, 917, 1145, 2276, 3107, 3246
XceI RCATGY 3 cut(s) 1290, 2248, 2523
XhoI CTCGAG 1 cut(s) 2777
XmiI GTMKAC 1 cut(s) 1835
XspI CTAG 4 cut(s) 713, 935, 1400, 3501
Zsp2I ATGCAT 1 cut(s) 3481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.