Rh3DG167100

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002940
Physical Location & Seq
Forward (+)
14529013 .. 14533116
4104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG167100.1

Sequence Viewer

Length: 3384 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGGGCGTTGATGATAGTGATGCAGTAGTGGGCGAGGAAACGAGTGAGGGTTTGGAAGAGGGAGATGAATTGGTAGATGGAGGTGATGATTCTGGAGGGAAAGTAGGACCTTTAGATATGGAGATTGATGAGAAAGGTGGAGATGGAGTGACAGAGGGTTTGGAGGGGACTCAATTTGGGGGTACTGATGCGAATGCAGCGAGTGAGAAAGGGTCGGTTTCGGAATCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAGTCTTTAGTGACAAGGATAGGGGTAAAGATGAGATTTTGAGGGAATGCGTGGAAGCTGAAGTTGTGGGTGCTGTGGAAGGGAATCGTTCAGTGAGTTTCGAGACGAAGGAGGATGGAACAGTTGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCGCAAGTAGATGAGGAAGAGAGAGGAAGAGAACAGAATGTGGAGCGTAGCCAGGGTGCAGAGAAGAGTGCCCATGCTACAAGTTCTAACGTGGAGCATGAGGCTACAGTGTCTGTTCGAGAAATACCGCAGGACATCCCAGCTGTGGTGGAAGTGAAAATTGCCGGTATCAATGAGTCTGGGAATGATGGAGACCTGAATGCAATCACTGGTTCCCAGATGGTTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGAAGTAAAGACCGGTGAAGATGTGTTGATGCCTGAATATGTTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGATTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCAGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTGTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTTTGGCATGGCCTGTTCTTGCATTTCCAAAGAAGTTTATGCAAAGCTCAAAACACAGACGATCATTAATGCTGGAATCAAGGAAGAAGCATGTAGAAGAGATGGTGGTGATAGTTCTCTGGGTGCTGCTTCTTTTGAACCCTTGAAACTCATTAACTTTGCAAAAGAAGCTGCCAAGTTGCCATTTGGCAGAGTTGACAGACTAGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTAGGTGGGTTGCTGGAAAATGATGCCGATATTTCACTATTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGATGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCAGAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGACAGTTCCCAACCAAGTAAAAAAGGGAAAAACTCCTCAAATGTAACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTGAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCAAGTGAGAAACGAAAGGCAGTTGATGGAGCTATGTCTGATGACTCTGCGGTGAAACATAGTAAAAGCGCTTCATCAGCAGGGGAAGATGTTTCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTGCTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAGATCTACATCGCAGAAACCACAAACTGAAAAGCTGATTGACATGGAGGATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTACTTGGCTGCCATGAATCCCAATAAGGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCAGTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTGGCAAGCGCAGTAAGAAATCAACCAAGACTGGAAAGAAATCAATCAAACCACGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTCTTATTCTTCTGATAAGATAGTTAAAAGTCAAAGTGAAAATGGACAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTATGGAGTCACAATCAAGTCCTGATTCGGAAGCACTGAAGCCAGTTCTTGACAATAAAAATGGAGAGTTGGTGTGTGGAACTGCTACAAGCAATAAGGATACTTCTACTGTTGAACTGCAAGCAAGTCTTGAGTTAAATCCCACTTTGGATTCTGAACAGAACAATGTTTGCGGAGATATTGAATCAAAAGCAGTGAACACAGCTGCTACAACCGATAAGGATACTTCTACTGTTGAACTGCAAGCAACTCTCGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGTTTGTGGAGATATTGATTCAAAAGCAGTGAACCCAGCTCTGGATGATGAAAATGGAGCATCAGTACGTGAGCCCATCTCTGTTGCTGAGTCATCAGCAGATCCCAAGTTAAATCCCAATTTGGATTTTGAACAGGAAAATACAGTTGGAGATACTAGTGCGGAAGCAGTGAAGCCAGCTCTTGACAATGAAAGTGGGGAGTTGGTGGCTGGAGCTCTAAGTGATAAGGATGCTTCCGCTGTGGATTCGCAAGCAAGTCTTGAGTTTAATCCCAATATGGATTCTGCACTGAAATTTCTTGATGGAGATCATGATTCAGAAGCAGTGAAGCCAGCTTCCCCAAGTGAGAGTTGTGAGGAAGATTCTCCCGCAGCTCTGATCTTGAACTTTTCAGACTTGGAGTCTCTTCCCTCGGAAGCAAATCTAAACAAAATTTTTAGCTGCTTCGGGCCTTTATATGAATCGCAAACCGAATTGTTGAAGAAGACTAACCGGGCCAAAGTGGTTTTTAGGCGGCGTTCTGATGCTCAAAAAGCTTTCAGCAGTGCCGGGAAATACAGTACCTTCGGACCTTCACTTGAAAGTTATCGCCTCAAGTATCCACTATCACCAAGTAAAGCTTCTCCTTCTCCGAAGGTGGCAAAGCAAGGAAAAATGGATGCATCTGCAGAAGGTGATGGAGCTCGGAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1127

Amino Acids

121.62

Weight (kDa)

4.67

Isoelectric Point (pI)

47.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 312 - 394 1.3e-16 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1975
Acc16I TGCGCA 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 1975
AccI GTMKAC 1 cut(s) 1712
AciI CCGC 7 cut(s) 605, 1823, 2571, 2849, 2925, 3057, 3202
AclWI GGATC 6 cut(s) 983, 1741, 2045, 2058, 2202, 2783
AcsI RAATTY 5 cut(s) 920, 1148, 2150, 2981, 3120
AcuI CTGAAG 4 cut(s) 387, 978, 1998, 2456
AfaI GTAC 5 cut(s) 235, 1066, 2114, 2754, 3250
AfeI AGCGCT 1 cut(s) 1843
AfiI CCNNNNNNNGG 6 cut(s) 622, 796, 863, 2108, 2109, 2728
AflIII ACRYGT 1 cut(s) 2393
AgeI ACCGGT 1 cut(s) 755
AhdI GACNNNNNGTC 1 cut(s) 3088
AhlI ACTAGT 1 cut(s) 2843
AjnI CCWGG 3 cut(s) 528, 973, 2169
AjuI GAANNNNNNNTTGG 4 cut(s) 2817, 2849, 3292, 3324
Alw21I GWGCWC 3 cut(s) 1174, 2905, 3373
Alw26I GTCTC 6 cut(s) 404, 663, 1189, 1641, 2079, 3096
AlwI GGATC 6 cut(s) 983, 1741, 2045, 2058, 2202, 2783
AlwNI CAGNNNCTG 3 cut(s) 590, 1967, 1976
Ama87I CYCGRG 1 cut(s) 2651
Aor51HI AGCGCT 1 cut(s) 1843
AoxI GGCC 5 cut(s) 971, 1209, 1422, 3137, 3183
ApaI GGGCCC 1 cut(s) 1426
ApoI RAATTY 5 cut(s) 920, 1148, 2150, 2981, 3120
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AseI ATTAAT 1 cut(s) 1266
AsiGI ACCGGT 1 cut(s) 755
AspLEI GCGC 3 cut(s) 481, 1844, 2232
AspS9I GGNCC 7 cut(s) 158, 905, 1422, 1423, 3137, 3183, 3257
AsuC2I CCSGG 2 cut(s) 3182, 3238
AsuHPI GGTGA 6 cut(s) 146, 770, 1319, 1837, 3288, 3374
AvaI CYCGRG 1 cut(s) 2651
AvaII GGWCC 3 cut(s) 158, 905, 3257
BaeGI GKGCMC 2 cut(s) 550, 1426
BaeI ACNNNNGTAYC 2 cut(s) 2382, 2415
BamHI GGATCC 1 cut(s) 2050
BanII GRGCYC 4 cut(s) 1426, 2763, 2905, 3373
BarI GAAGNNNNNNTAC 2 cut(s) 1828, 1860
BbsI GAAGAC 1 cut(s) 3179
Bbv12I GWGCWC 3 cut(s) 1174, 2905, 3373
BciT130I CCWGG 3 cut(s) 530, 975, 2171
BciVI GTATCC 3 cut(s) 2491, 2614, 3297
BcnI CCSGG 2 cut(s) 3182, 3238
BcoDI GTCTC 6 cut(s) 404, 663, 1189, 1641, 2079, 3096
BcuI ACTAGT 1 cut(s) 2843
BfaI CTAG 5 cut(s) 716, 938, 1403, 1484, 2844
BfmI CTRYAG 4 cut(s) 582, 1909, 1968, 3354
BfoI RGCGCY 1 cut(s) 1845
BfuAI ACCTGC 1 cut(s) 1975
BfuI GTATCC 3 cut(s) 2491, 2614, 3297
BglII AGATCT 1 cut(s) 2003
Bme1390I CCNGG 5 cut(s) 530, 975, 2171, 3182, 3238
Bme18I GGWCC 3 cut(s) 158, 905, 3257
BmeRI GACNNNNNGTC 1 cut(s) 3088
BmeT110I CYCGRG 1 cut(s) 2651
BmgT120I GGNCC 7 cut(s) 158, 905, 1422, 1423, 3137, 3183, 3257
BmiI GGNNCC 7 cut(s) 691, 855, 907, 959, 1424, 2052, 2175
BmrFI CCNGG 5 cut(s) 530, 975, 2171, 3182, 3238
BmrI ACTGGG 1 cut(s) 2175
BmuI ACTGGG 1 cut(s) 2175
BpiI GAAGAC 1 cut(s) 3179
BplI GAGNNNNNCTC 2 cut(s) 2750, 2782
BpmI CTGGAG 3 cut(s) 165, 1665, 2919
BpuEI CTTGAG 5 cut(s) 1171, 1759, 2549, 2969, 3266
BpuMI CCSGG 2 cut(s) 3182, 3238
BsaAI YACGTR 1 cut(s) 2756
BsaBI GATNNNNATC 3 cut(s) 2008, 2193, 2994
BsaI GGTCTC 1 cut(s) 663
BsaJI CCNNGG 4 cut(s) 529, 965, 974, 3099
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 8 cut(s) 138, 168, 183, 213, 2454, 2484, 2736, 2766
Bsc4I CCNNNNNNNGG 6 cut(s) 622, 796, 863, 2108, 2109, 2728
Bse118I RCCGGY 2 cut(s) 641, 755
Bse1I ACTGG 5 cut(s) 691, 1568, 2181, 2257, 2441
Bse3DI GCAATG 1 cut(s) 1619
Bse8I GATNNNNATC 3 cut(s) 2008, 2193, 2994
BseBI CCWGG 3 cut(s) 530, 975, 2171
BseDI CCNNGG 4 cut(s) 529, 965, 974, 3099
BseJI GATNNNNATC 3 cut(s) 2008, 2193, 2994
BseLI CCNNNNNNNGG 6 cut(s) 622, 796, 863, 2108, 2109, 2728
BseMI GCAATG 1 cut(s) 1619
BseMII CTCAG 5 cut(s) 1475, 1689, 2090, 2283, 2766
BseNI ACTGG 5 cut(s) 691, 1568, 2181, 2257, 2441
BseRI GAGGAG 3 cut(s) 60, 1606, 1672
BseSI GKGCMC 2 cut(s) 550, 1426
BseYI CCCAGC 3 cut(s) 616, 790, 2722
BsgI GTGCAG 2 cut(s) 555, 2958
BshFI GGCC 5 cut(s) 973, 1211, 1424, 3139, 3185
BshTI ACCGGT 1 cut(s) 755
BsiHKAI GWGCWC 3 cut(s) 1174, 2905, 3373
BsiHKCI CYCGRG 1 cut(s) 2651
BsiSI CCGG 4 cut(s) 642, 756, 3181, 3237
BslFI GGGAC 3 cut(s) 232, 891, 2223
BslI CCNNNNNNNGG 6 cut(s) 622, 796, 863, 2108, 2109, 2728
BsmAI GTCTC 6 cut(s) 404, 663, 1189, 1641, 2079, 3096
BsmBI CGTCTC 1 cut(s) 404
BsmFI GGGAC 3 cut(s) 232, 891, 2223
BsmI GAATGC 3 cut(s) 250, 359, 682
BsnI GGCC 5 cut(s) 973, 1211, 1424, 3139, 3185
Bso31I GGTCTC 1 cut(s) 663
BsoBI CYCGRG 1 cut(s) 2651
Bsp120I GGGCCC 1 cut(s) 1422
Bsp1286I GDGCHC 6 cut(s) 550, 1174, 1426, 2763, 2905, 3373
Bsp1407I TGTACA 1 cut(s) 1064
Bsp19I CCATGG 1 cut(s) 965
BspACI CCGC 7 cut(s) 605, 1823, 2571, 2849, 2925, 3057, 3202
BspANI GGCC 5 cut(s) 973, 1211, 1424, 3139, 3185
BspCNI CTCAG 5 cut(s) 1474, 1690, 2089, 2284, 2767
BspHI TCATGA 1 cut(s) 2998
BspLI GGNNCC 7 cut(s) 691, 855, 907, 959, 1424, 2052, 2175
BspMAI CTGCAG 2 cut(s) 1972, 3358
BspMI ACCTGC 1 cut(s) 1975
BspPI GGATC 6 cut(s) 983, 1741, 2045, 2058, 2202, 2783
BspTNI GGTCTC 1 cut(s) 663
BsrDI GCAATG 1 cut(s) 1619
BsrFI RCCGGY 2 cut(s) 641, 755
BsrGI TGTACA 1 cut(s) 1064
BsrI ACTGG 5 cut(s) 691, 1568, 2181, 2257, 2441
BssAI RCCGGY 2 cut(s) 641, 755
BssECI CCNNGG 4 cut(s) 529, 965, 974, 3099
BssT1I CCWWGG 1 cut(s) 965
Bst2UI CCWGG 3 cut(s) 530, 975, 2171
Bst6I CTCTTC 7 cut(s) 102, 462, 489, 499, 536, 1291, 3099
BstAPI GCANNNNNTGC 1 cut(s) 1967
BstAUI TGTACA 1 cut(s) 1064
BstBAI YACGTR 1 cut(s) 2756
BstC8I GCNNGC 7 cut(s) 2228, 2380, 2520, 2643, 2865, 2940, 3021
BstDEI CTNAG 7 cut(s) 291, 1461, 1698, 2076, 2292, 2775, 2906
BstDSI CCRYGG 1 cut(s) 965
BstH2I RGCGCY 1 cut(s) 1845
BstHHI GCGC 3 cut(s) 481, 1844, 2232
BstMAI GTCTC 6 cut(s) 404, 663, 1189, 1641, 2079, 3096
BstMWI GCNNNNNNNGC 6 cut(s) 248, 1367, 1850, 1967, 2077, 3221
BstNI CCWGG 3 cut(s) 530, 975, 2171
BstNSI RCATGY 3 cut(s) 1293, 2122, 2397
BstSCI CCNGG 5 cut(s) 528, 973, 2169, 3180, 3236
BstSFI CTRYAG 4 cut(s) 582, 1909, 1968, 3354
BstSLI GKGCMC 2 cut(s) 550, 1426
BstV2I GAAGAC 1 cut(s) 3179
BstX2I RGATCY 3 cut(s) 2003, 2050, 2788
BstYI RGATCY 3 cut(s) 2003, 2050, 2788
BsuI GTATCC 3 cut(s) 2491, 2614, 3297
BsuRI GGCC 5 cut(s) 973, 1211, 1424, 3139, 3185
BtgI CCRYGG 1 cut(s) 965
BtgZI GCGATG 1 cut(s) 1995
BtsI GCAGTG 5 cut(s) 2598, 2721, 2862, 3018, 3238
BveI ACCTGC 1 cut(s) 1975
Cac8I GCNNGC 7 cut(s) 2228, 2380, 2520, 2643, 2865, 2940, 3021
CaiI CAGNNNCTG 3 cut(s) 590, 1967, 1976
CciI TCATGA 1 cut(s) 2998
CfoI GCGC 3 cut(s) 481, 1844, 2232
Cfr10I RCCGGY 2 cut(s) 641, 755
Cfr13I GGNCC 7 cut(s) 158, 905, 1422, 1423, 3137, 3183, 3257
Csp6I GTAC 5 cut(s) 234, 1065, 2113, 2753, 3249
CspAI ACCGGT 1 cut(s) 755
CspCI CAANNNNNGTGG 2 cut(s) 2863, 2898
CviQI GTAC 5 cut(s) 234, 1065, 2113, 2753, 3249
DdeI CTNAG 7 cut(s) 291, 1461, 1698, 2076, 2292, 2775, 2906
DriI GACNNNNNGTC 1 cut(s) 3088
Eam1104I CTCTTC 7 cut(s) 102, 462, 489, 499, 536, 1291, 3099
Eam1105I GACNNNNNGTC 1 cut(s) 3088
EarI CTCTTC 7 cut(s) 102, 462, 489, 499, 536, 1291, 3099
Ecl136II GAGCTC 2 cut(s) 2903, 3371
Eco130I CCWWGG 1 cut(s) 965
Eco24I GRGCYC 4 cut(s) 1426, 2763, 2905, 3373
Eco31I GGTCTC 1 cut(s) 663
Eco47I GGWCC 3 cut(s) 158, 905, 3257
Eco47III AGCGCT 1 cut(s) 1843
Eco53kI GAGCTC 2 cut(s) 2903, 3371
Eco57I CTGAAG 4 cut(s) 387, 978, 1998, 2456
Eco88I CYCGRG 1 cut(s) 2651
EcoICRI GAGCTC 2 cut(s) 2903, 3371
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1148
EcoRII CCWGG 3 cut(s) 528, 973, 2169
EcoT14I CCWWGG 1 cut(s) 965
EcoT22I ATGCAT 1 cut(s) 3352
EcoT38I GRGCYC 4 cut(s) 1426, 2763, 2905, 3373
ErhI CCWWGG 1 cut(s) 965
Esp3I CGTCTC 1 cut(s) 404
FalI AAGNNNNNCTT 8 cut(s) 1548, 1580, 2511, 2543, 2931, 2963, 3292, 3324
FaqI GGGAC 3 cut(s) 232, 891, 2223
FauI CCCGC 1 cut(s) 3064
FblI GTMKAC 1 cut(s) 1712
FriOI GRGCYC 4 cut(s) 1426, 2763, 2905, 3373
FspBI CTAG 5 cut(s) 716, 938, 1403, 1484, 2844
FspI TGCGCA 1 cut(s) 480
GlaI GCGC 3 cut(s) 480, 1843, 2231
GsaI CCCAGC 3 cut(s) 620, 794, 2726
GsuI CTGGAG 3 cut(s) 165, 1665, 2919
HaeII RGCGCY 1 cut(s) 1845
HaeIII GGCC 5 cut(s) 973, 1211, 1424, 3139, 3185
HapII CCGG 4 cut(s) 642, 756, 3181, 3237
HhaI GCGC 3 cut(s) 481, 1844, 2232
Hin6I GCGC 3 cut(s) 479, 1842, 2230
HinP1I GCGC 3 cut(s) 479, 1842, 2230
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HindIII AAGCTT 3 cut(s) 927, 3222, 3306
HpaII CCGG 4 cut(s) 642, 756, 3181, 3237
HphI GGTGA 6 cut(s) 146, 770, 1319, 1837, 3288, 3374
Hpy166II GTNNAC 5 cut(s) 713, 1396, 1713, 2596, 2719
Hpy8I GTNNAC 5 cut(s) 713, 1396, 1713, 2596, 2719
HpyCH4IV ACGT 3 cut(s) 567, 728, 2755
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 1367, 1850, 1967, 2077, 3221
HpyF3I CTNAG 7 cut(s) 291, 1461, 1698, 2076, 2292, 2775, 2906
HpySE526I ACGT 3 cut(s) 567, 728, 2755
HspAI GCGC 3 cut(s) 479, 1842, 2230
MaeI CTAG 5 cut(s) 716, 938, 1403, 1484, 2844
MaeII ACGT 3 cut(s) 567, 728, 2755
MaeIII GTNAC 5 cut(s) 199, 317, 1411, 1690, 2406
MfeI CAATTG 1 cut(s) 2198
MflI RGATCY 3 cut(s) 2003, 2050, 2788
MhlI GDGCHC 6 cut(s) 550, 1174, 1426, 2763, 2905, 3373
MlyI GAGTC 7 cut(s) 214, 662, 1811, 2306, 2414, 2786, 3098
MmeI TCCRAC 5 cut(s) 711, 1427, 1876, 2277, 2815
Mph1103I ATGCAT 1 cut(s) 3352
MspA1I CMGCKG 4 cut(s) 620, 1465, 2603, 2927
MspI CCGG 4 cut(s) 642, 756, 3181, 3237
MspR9I CCNGG 5 cut(s) 530, 975, 2171, 3182, 3238
MunI CAATTG 1 cut(s) 2198
Mva1269I GAATGC 3 cut(s) 250, 359, 682
MvaI CCWGG 3 cut(s) 530, 975, 2171
MwoI GCNNNNNNNGC 6 cut(s) 248, 1367, 1850, 1967, 2077, 3221
NciI CCSGG 2 cut(s) 3182, 3238
NcoI CCATGG 1 cut(s) 965
NlaIV GGNNCC 7 cut(s) 691, 855, 907, 959, 1424, 2052, 2175
NmuCI GTSAC 3 cut(s) 199, 317, 2406
NsbI TGCGCA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 3352
NspI RCATGY 3 cut(s) 1293, 2122, 2397
PaeR7I CTCGAG 1 cut(s) 2651
PagI TCATGA 1 cut(s) 2998
PaqCI CACCTGC 1 cut(s) 1975
PciI ACATGT 1 cut(s) 2393
PctI GAATGC 3 cut(s) 250, 359, 682
PinAI ACCGGT 1 cut(s) 755
PleI GAGTC 7 cut(s) 214, 661, 1811, 2306, 2413, 2785, 3097
PpsI GAGTC 7 cut(s) 214, 661, 1811, 2306, 2413, 2785, 3097
Ppu21I YACGTR 1 cut(s) 2756
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2393
PshBI ATTAAT 1 cut(s) 1266
Psp124BI GAGCTC 2 cut(s) 2905, 3373
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 528, 973, 2169
PspFI CCCAGC 3 cut(s) 616, 790, 2722
PspGI CCWGG 3 cut(s) 528, 973, 2169
PspN4I GGNNCC 7 cut(s) 691, 855, 907, 959, 1424, 2052, 2175
PspOMI GGGCCC 1 cut(s) 1422
PspPI GGNCC 7 cut(s) 158, 905, 1422, 1423, 3137, 3183, 3257
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 1972, 3358
PstNI CAGNNNCTG 3 cut(s) 590, 1967, 1976
PsuI RGATCY 3 cut(s) 2003, 2050, 2788
PvuII CAGCTG 3 cut(s) 620, 1465, 2603
RsaI GTAC 5 cut(s) 235, 1066, 2114, 2754, 3250
RsaNI GTAC 5 cut(s) 234, 1065, 2113, 2753, 3249
SacI GAGCTC 2 cut(s) 2905, 3373
Sau96I GGNCC 7 cut(s) 158, 905, 1422, 1423, 3137, 3183, 3257
SchI GAGTC 7 cut(s) 214, 662, 1811, 2306, 2414, 2786, 3098
ScrFI CCNGG 5 cut(s) 530, 975, 2171, 3182, 3238
SduI GDGCHC 6 cut(s) 550, 1174, 1426, 2763, 2905, 3373
SfcI CTRYAG 4 cut(s) 582, 1909, 1968, 3354
Sfr274I CTCGAG 1 cut(s) 2651
SinI GGWCC 3 cut(s) 158, 905, 3257
SlaI CTCGAG 1 cut(s) 2651
SmlI CTYRAG 6 cut(s) 1186, 1774, 2528, 2651, 2948, 3281
SmoI CTYRAG 6 cut(s) 1186, 1774, 2528, 2651, 2948, 3281
SpeI ACTAGT 1 cut(s) 2843
SsiI CCGC 7 cut(s) 605, 1823, 2571, 2849, 2925, 3057, 3202
SspI AATATT 2 cut(s) 1947, 2217
SspMI CTAG 5 cut(s) 716, 938, 1403, 1484, 2844
SstI GAGCTC 2 cut(s) 2905, 3373
StyD4I CCNGG 5 cut(s) 528, 973, 2169, 3180, 3236
StyI CCWWGG 1 cut(s) 965
TaiI ACGT 3 cut(s) 570, 731, 2758
TaqI TCGA 4 cut(s) 278, 408, 595, 2652
TatI WGTACW 1 cut(s) 1064
TauI GCSGC 1 cut(s) 3205
TseFI GTSAC 3 cut(s) 199, 317, 2406
Tsp45I GTSAC 3 cut(s) 199, 317, 2406
VpaK11BI GGWCC 3 cut(s) 158, 905, 3257
VspI ATTAAT 1 cut(s) 1266
XapI RAATTY 5 cut(s) 920, 1148, 2150, 2981, 3120
XceI RCATGY 3 cut(s) 1293, 2122, 2397
XhoI CTCGAG 1 cut(s) 2651
XmiI GTMKAC 1 cut(s) 1712
XspI CTAG 5 cut(s) 716, 938, 1403, 1484, 2844
Zsp2I ATGCAT 1 cut(s) 3352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.