pycom12g12280

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002940
Physical Location & Seq
Reverse (-)
14773882 .. 14776695
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g12280.1

Sequence Viewer

Length: 2814 bp
ATGGACCTGAACGCCGACGTCGTTTTGGCTGACCCGAGTGACGGGGTTCACCACTCTGAGCCGGCGCTGGGAGTGGTGGGCGAAGTGAATGAAATGGCTTGTGTGGGAGGTGGAGTGAATGGGGGTGTAGAGGAGAAAGGTGAAGTGGTGGATGGAGGTGATGATTCTGAATTGAAATTAGGACTGTTAGATGGGGATGTTGAGGGGAAAGGGAGCATTGTTTCGTCAGAGGTGGCGGTTAATGGCTGGGAAGGTAGAGAGGGCTTGGAGGGACCGCAATTTGGTGCTGGGGGTGTGGTTGGAGAAAATGTTAATAGGTTGGTTTTGAAATCTGAGGATGGTTTGAGGAACGTTGAAACCAGAAGGGGTAGAGGGGAGGATGCGACTTTAGGGGAATGGACAAAAGATGAAGTTGTGGGTTCTGAGGATGGAGATTTGGTGAGTGTTAAGGCCAAGCAGAATGTGGCAGTTCATGGAAGGGATGACATCATGATAGATGAGAAAGAAGAGGACGTTGTTCGAGTAGCTGATATAGCTCCTGCTGAAATAGAAATGGATCAAAAGGTGGAGCAGGGCCAAGTTGAAGACCAGAGTCTTGATGCTTCCAGTTCTATGCGTGGCAATGGTGACATTGAGTATTTGGAAACTGTTGGACAAATAACAAAAGGCACCCCGGCTATGGAAGTGAAAGTTGTTGATCATAGTGAGTCTTTGGATAAAAAAGACCTGAATGTAAGCACGCAGTCTAAGATGGAAACCTGTGATGGACTAGAAAATCAGCCCATGGAAGTTAATGTGGAAGCACAGACAAATGAGAACAAATTGACCCGTGATGTTTCAGAGAATGTGGTTCGGCAGAATACTGAAAATGGAAAATCTACTTCCTCAGATGGGAATGTGTCAAGTGATAGAAATGGAAAGTCTGCTTCCACAGAACTAGAATTCCGTGATTCTGATCTAGTATGGGGAAAGGTAAGGAGTCATCCCTGGTGGCCTGGGCAGATCTGTGATCCTTCAGCTGCATCAGTGAAGGCAAAGAAGTATTTTAAAAGAGGCACTTATCTAATAGCGTATTATTGGGATAATACATTTGCTTGGAATGAAGCAATGCGGATTAAGCCCTTCTTTGAGAACTTTTCACAAATGGAGAAACAAAGCAACATGGAAGAATTCCACAATGCAATTGGTTGTGCTTTGGATGAAGTCTCAAGGCGGGTAGAATTAGGGCTGAGCTGTTCTTGCATATCCAAAGAAGTATATGAAAAACTCAAAACTCAGATAATTGAAAATGCTGGAATCAGTGAAGAAGCAAGTAGAAGAGATGGTGGCGACAAGTCTTTAAGTGCTGCTTCTTTTGAACCTGTGAAACTCATTAGGTTTGTAAAGAAGTTGGCTCAGTTTCCATATAGAAAAGTTGACAGACTAGAACTTGTGACATCAAGGGCCCAATTATCAGCCTTCAATTGCTGGAAGGGTTGTCCTCCACTGCCAGAGTTCAATATACTTGGTGGGTTGCTGACCGATGCTGAAATATTACAATTGGGAGAGAAGCAGTGTGCTGAAGCAAGTGAAAATGCACTTCCAGTAATTAAGGATGAGGATTTGGGGCCGCAATCAAAAAGTAAAGACAACTTACCGCAGAAGCGCAAGAATATGTCTGGAGATAGTATGCGCCCGAGTAAAAAAGAGAGAAGTATACCAGATGTAGTGGCTGAGAAGTATTTGTCTACTCCAGTGAGTGAGAAAGGATTGGAAGGGAAAGTGGGCAGTAAACTGATTTCACAATCTTCCAGTAAGAAACGAAAGGCAGCTGATGCTACATCTGATGACTCGGCAGTAAAGCAACGGAAAATTGAGTCGTCGACAGGGGCTGATAGTAATTCTATACAAAATAAACAGACTTTTAAAGTTGGAGATAGGATACGCAGGGTTGCAAGCCAACTAAGTGGATTGAGCTCAATACTGAAGAATTACAGTGCAACATCTGGAGCAGTTGAAGTTGAGGAAGGCAAAGGAACTGAGTACCCATCTCCTGATAAGATGCTGTCTCAGGTCTGCTTGGCGGCTATAGATCCCATGAATGGATACAGCTTCTTGAGTTCCATGATTACTTGCTTCCAGGAATTTAGGAATACTATATGCTTGGACCATTCCAATCCAAAGGAGGATCAAATGTCTCTGGAACAAATGTTTGGTGGTAAGCTGGTTAAGAAATCAATCAGGAGTGGGAAGAAATCAATGAGCTCCGGCATTACTGAAAAATCCAAGTCACAAGAAATTCCTCCTGAGCAATCATCACTCAAGAGTCGAAATGAAAATGAAGGATTAGTGCCTGGAGCTCCAAGTGATATAGAGACCTCTACTGATGAACAGCAAGCAAGTCTTGAGTCGGATCCCAATTTGGATTCTGAACAGAAAATTGCTGGTGGAGATATTGAGTCAGAAACAGTGAAGCCAGCTCTTGTCTCAAGTGAGAGTTGTGATGAATATCTCTCTCGGACAGCTCTGATCTTGAAATTTTCAGATTTGGAGTCCGTCCCTTCAGAGAAAAACCTGAAGAAAATATTTAGCCGCTATGGACCCTTGACTGAAGTGATGAGGAATAACCGCCGGGCCACAGTGGTTTTCGAGAAACGTTCTGATGCTGAAACAGCTTTCAGCAGCACAGGGAAATACAGTACTTTTGGACCTTCACTTGTCGGTTACAGCCTCAAGTACCTGCCGTCTAAACCAAGTAAAGCTGCTTCTTCAACCAATGCGAAAAAGCGTGGCAGAAAATGTGCAACAGCACCTGGAGGCAATGCAACTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

938

Amino Acids

101.92

Weight (kDa)

5.09

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 319 - 401 7.4e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 2727
AccB1I GGYRCC 1 cut(s) 668
AccI GTMKAC 3 cut(s) 1696, 1727, 1862
AciI CCGC 9 cut(s) 236, 275, 1111, 1213, 1610, 1637, 2063, 2572, 2608
AclI AACGTT 2 cut(s) 351, 2635
AclWI GGATC 6 cut(s) 564, 1004, 2066, 2175, 2387, 2400
AcsI RAATTY 5 cut(s) 941, 1169, 2123, 2277, 2516
AcuI CTGAAG 6 cut(s) 999, 1581, 1985, 2526, 2576, 2610
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 3 cut(s) 2024, 2680, 2717
AfiI CCNNNNNNNGG 6 cut(s) 41, 68, 281, 679, 891, 2081
AjnI CCWGG 5 cut(s) 986, 994, 2118, 2332, 2791
AjuI GAANNNNNNNTTGG 2 cut(s) 2175, 2207
AloI GAACNNNNNNTCC 2 cut(s) 927, 959
Alw21I GWGCWC 3 cut(s) 1958, 2246, 2341
Alw26I GTCTC 5 cut(s) 1210, 2052, 2181, 2348, 2470
AlwI GGATC 6 cut(s) 564, 1004, 2066, 2175, 2387, 2400
AlwNI CAGNNNCTG 2 cut(s) 1871, 2792
Ama87I CYCGRG 2 cut(s) 34, 1675
AoxI GGCC 6 cut(s) 450, 574, 992, 1443, 1607, 2613
ApaI GGGCCC 1 cut(s) 1447
ApeKI GCWGC 5 cut(s) 1019, 1346, 1808, 2661, 2741
ApoI RAATTY 5 cut(s) 941, 1169, 2123, 2277, 2516
AspLEI GCGC 3 cut(s) 67, 1647, 1674
AsuC2I CCSGG 2 cut(s) 674, 2612
AsuHPI GGTGA 5 cut(s) 41, 152, 170, 451, 638
AvaI CYCGRG 2 cut(s) 34, 1675
AvaII GGWCC 5 cut(s) 4, 272, 2146, 2579, 2687
BaeGI GKGCMC 1 cut(s) 1447
BamHI GGATCC 1 cut(s) 2392
BanI GGYRCC 1 cut(s) 668
BanII GRGCYC 4 cut(s) 1447, 1958, 2246, 2341
BarI GAAGNNNNNNTAC 2 cut(s) 2728, 2760
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 3 cut(s) 1958, 2246, 2341
BbvI GCAGC 5 cut(s) 1006, 1333, 1820, 2673, 2728
BccI CCATC 9 cut(s) 146, 185, 332, 422, 745, 758, 884, 1316, 2035
BceAI ACGGC 1 cut(s) 2707
BciT130I CCWGG 5 cut(s) 988, 996, 2120, 2334, 2793
BciVI GTATCC 2 cut(s) 1914, 2078
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 2 cut(s) 674, 2612
BcoDI GTCTC 5 cut(s) 1210, 2052, 2181, 2348, 2470
BfaI CTAG 4 cut(s) 770, 938, 959, 1424
BfmI CTRYAG 1 cut(s) 2067
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 1 cut(s) 2727
BfuI GTATCC 2 cut(s) 1914, 2078
BglII AGATCT 1 cut(s) 1002
BisI GCNGC 8 cut(s) 1020, 1347, 1610, 1809, 2064, 2572, 2662, 2742
BlpI GCTNAGC 1 cut(s) 1229
BlsI GCNGC 8 cut(s) 1021, 1348, 1611, 1810, 2065, 2573, 2663, 2743
BmcAI AGTACT 1 cut(s) 2680
Bme1390I CCNGG 7 cut(s) 674, 988, 996, 2120, 2334, 2612, 2793
Bme18I GGWCC 5 cut(s) 4, 272, 2146, 2579, 2687
BmeT110I CYCGRG 2 cut(s) 34, 1675
BmiI GGNNCC 6 cut(s) 273, 670, 1445, 1608, 2394, 2581
BmrFI CCNGG 7 cut(s) 674, 988, 996, 2120, 2334, 2612, 2793
BmsI GCATC 7 cut(s) 370, 589, 1031, 1513, 1804, 2031, 2632
BoxI GACNNNNGTC 1 cut(s) 591
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 5 cut(s) 1680, 1716, 2007, 2355, 2814
Bpu10I CCTNAGC 1 cut(s) 2286
Bpu1102I GCTNAGC 1 cut(s) 1229
BpuEI CTTGAG 6 cut(s) 1192, 2116, 2285, 2405, 2452, 2696
BpuMI CCSGG 2 cut(s) 674, 2612
BsaBI GATNNNNATC 2 cut(s) 954, 2487
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 2348
BsaJI CCNNGG 4 cut(s) 672, 783, 986, 995
BsaXI ACNNNNNCTCC 6 cut(s) 99, 129, 294, 324, 423, 453
Bsc4I CCNNNNNNNGG 6 cut(s) 41, 68, 281, 679, 891, 2081
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 4 cut(s) 606, 1583, 1733, 1791
Bse3DI GCAATG 3 cut(s) 628, 1113, 2806
Bse8I GATNNNNATC 2 cut(s) 954, 2487
BseBI CCWGG 5 cut(s) 988, 996, 2120, 2334, 2793
BseDI CCNNGG 4 cut(s) 672, 783, 986, 995
BseGI GGATG 9 cut(s) 157, 202, 343, 385, 433, 487, 982, 1204, 1600
BseJI GATNNNNATC 2 cut(s) 954, 2487
BseLI CCNNNNNNNGG 6 cut(s) 41, 68, 281, 679, 891, 2081
BseMI GCAATG 3 cut(s) 628, 1113, 2806
BseNI ACTGG 4 cut(s) 606, 1583, 1733, 1791
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 1447
BseXI GCAGC 5 cut(s) 1006, 1333, 1820, 2673, 2728
BseYI CCCAGC 3 cut(s) 67, 246, 287
BshFI GGCC 6 cut(s) 452, 576, 994, 1445, 1609, 2615
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 3 cut(s) 1958, 2246, 2341
BsiHKCI CYCGRG 2 cut(s) 34, 1675
BsiSI CCGG 4 cut(s) 62, 674, 2247, 2611
BslFI GGGAC 2 cut(s) 285, 2522
BslI CCNNNNNNNGG 6 cut(s) 41, 68, 281, 679, 891, 2081
BsmAI GTCTC 5 cut(s) 1210, 2052, 2181, 2348, 2470
BsmFI GGGAC 2 cut(s) 285, 2522
BsnI GGCC 6 cut(s) 452, 576, 994, 1445, 1609, 2615
Bso31I GGTCTC 1 cut(s) 2348
BsoBI CYCGRG 2 cut(s) 34, 1675
Bsp120I GGGCCC 1 cut(s) 1443
Bsp1286I GDGCHC 4 cut(s) 1447, 1958, 2246, 2341
Bsp143I GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
Bsp1720I GCTNAGC 1 cut(s) 1229
Bsp19I CCATGG 1 cut(s) 783
BspACI CCGC 9 cut(s) 236, 275, 1111, 1213, 1610, 1637, 2063, 2572, 2608
BspANI GGCC 6 cut(s) 452, 576, 994, 1445, 1609, 2615
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 6 cut(s) 273, 670, 1445, 1608, 2394, 2581
BspMI ACCTGC 1 cut(s) 2727
BspPI GGATC 6 cut(s) 564, 1004, 2066, 2175, 2387, 2400
BspT107I GGYRCC 1 cut(s) 668
BspTNI GGTCTC 1 cut(s) 2348
BsrDI GCAATG 3 cut(s) 628, 1113, 2806
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 4 cut(s) 606, 1583, 1733, 1791
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 4 cut(s) 672, 783, 986, 995
BssMI GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
BssNAI GTATAC 1 cut(s) 1697
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 783
Bst1107I GTATAC 1 cut(s) 1697
Bst2UI CCWGG 5 cut(s) 988, 996, 2120, 2334, 2793
Bst4CI ACNGT 6 cut(s) 186, 649, 1976, 2449, 2620, 2678
Bst6I CTCTTC 2 cut(s) 501, 1312
BstACI GRCGYC 1 cut(s) 18
BstAPI GCANNNNNTGC 1 cut(s) 1814
BstC8I GCNNGC 5 cut(s) 63, 740, 1936, 2376, 2457
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 9 cut(s) 157, 202, 343, 385, 433, 487, 982, 1204, 1600
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 3 cut(s) 67, 1647, 1674
BstKTI GATC 9 cut(s) 559, 700, 958, 1005, 1012, 2074, 2170, 2395, 2511
BstMAI GTCTC 5 cut(s) 1210, 2052, 2181, 2348, 2470
BstMBI GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
BstMWI GCNNNNNNNGC 5 cut(s) 533, 1117, 1239, 1814, 2651
BstNI CCWGG 5 cut(s) 988, 996, 2120, 2334, 2793
BstPAI GACNNNNGTC 1 cut(s) 591
BstSCI CCNGG 7 cut(s) 672, 986, 994, 2118, 2332, 2610, 2791
BstSFI CTRYAG 1 cut(s) 2067
BstSLI GKGCMC 1 cut(s) 1447
BstV1I GCAGC 5 cut(s) 1006, 1333, 1820, 2673, 2728
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 3 cut(s) 1002, 2071, 2392
BstXI CCANNNNNNTGG 1 cut(s) 1946
BstYI RGATCY 3 cut(s) 1002, 2071, 2392
BstZ17I GTATAC 1 cut(s) 1697
BsuI GTATCC 2 cut(s) 1914, 2078
BsuRI GGCC 6 cut(s) 452, 576, 994, 1445, 1609, 2615
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 9 cut(s) 157, 202, 343, 385, 433, 487, 982, 1204, 1600
BtsI GCAGTG 2 cut(s) 1484, 1559
BtsIMutI CAGTG 8 cut(s) 1032, 1306, 1484, 1559, 1740, 1981, 2454, 2625
BveI ACCTGC 1 cut(s) 2727
Cac8I GCNNGC 5 cut(s) 63, 740, 1936, 2376, 2457
CaiI CAGNNNCTG 2 cut(s) 1871, 2792
CciI TCATGA 1 cut(s) 489
CfoI GCGC 3 cut(s) 67, 1647, 1674
Cfr10I RCCGGY 1 cut(s) 61
Csp6I GTAC 3 cut(s) 2023, 2679, 2716
CviAII CATG 6 cut(s) 473, 490, 784, 1162, 2077, 2104
CviQI GTAC 3 cut(s) 2023, 2679, 2716
DpnI GATC 9 cut(s) 558, 699, 957, 1004, 1011, 2073, 2169, 2394, 2510
DpnII GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
DraI TTTAAA 2 cut(s) 1048, 1906
Eam1104I CTCTTC 2 cut(s) 501, 1312
EarI CTCTTC 2 cut(s) 501, 1312
Ecl136II GAGCTC 3 cut(s) 1956, 2244, 2339
Eco130I CCWWGG 1 cut(s) 783
Eco24I GRGCYC 4 cut(s) 1447, 1958, 2246, 2341
Eco31I GGTCTC 1 cut(s) 2348
Eco47I GGWCC 5 cut(s) 4, 272, 2146, 2579, 2687
Eco53kI GAGCTC 3 cut(s) 1956, 2244, 2339
Eco57I CTGAAG 6 cut(s) 999, 1581, 1985, 2526, 2576, 2610
Eco88I CYCGRG 2 cut(s) 34, 1675
EcoICRI GAGCTC 3 cut(s) 1956, 2244, 2339
EcoO109I RGGNCCY 1 cut(s) 1443
EcoRI GAATTC 2 cut(s) 941, 1169
EcoRII CCWGG 5 cut(s) 986, 994, 2118, 2332, 2791
EcoT14I CCWWGG 1 cut(s) 783
EcoT38I GRGCYC 4 cut(s) 1447, 1958, 2246, 2341
ErhI CCWWGG 1 cut(s) 783
FaeI CATG 6 cut(s) 476, 493, 787, 1165, 2080, 2107
FalI AAGNNNNNCTT 8 cut(s) 1042, 1074, 1109, 1141, 1333, 1365, 2367, 2399
FaqI GGGAC 2 cut(s) 285, 2522
FatI CATG 6 cut(s) 472, 489, 783, 1161, 2076, 2103
FauI CCCGC 1 cut(s) 1206
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 3 cut(s) 1696, 1727, 1862
Fnu4HI GCNGC 8 cut(s) 1020, 1347, 1610, 1809, 2064, 2572, 2662, 2742
FokI GGATG 9 cut(s) 164, 209, 350, 392, 440, 494, 969, 1211, 1607
FriOI GRGCYC 4 cut(s) 1447, 1958, 2246, 2341
Fsp4HI GCNGC 8 cut(s) 1020, 1347, 1610, 1809, 2064, 2572, 2662, 2742
FspBI CTAG 4 cut(s) 770, 938, 959, 1424
GlaI GCGC 3 cut(s) 66, 1646, 1673
GluI GCNGC 8 cut(s) 1020, 1347, 1610, 1809, 2064, 2572, 2662, 2742
GsaI CCCAGC 3 cut(s) 71, 250, 291
GsuI CTGGAG 5 cut(s) 1680, 1716, 2007, 2355, 2814
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 6 cut(s) 452, 576, 994, 1445, 1609, 2615
HapII CCGG 4 cut(s) 62, 674, 2247, 2611
HhaI GCGC 3 cut(s) 67, 1647, 1674
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 6 cut(s) 476, 493, 787, 1165, 2080, 2107
Hin6I GCGC 3 cut(s) 65, 1645, 1672
HinP1I GCGC 3 cut(s) 65, 1645, 1672
HincII GTYRAC 2 cut(s) 1417, 1863
HindII GTYRAC 2 cut(s) 1417, 1863
HpaII CCGG 4 cut(s) 62, 674, 2247, 2611
HphI GGTGA 5 cut(s) 41, 152, 170, 451, 638
Hpy166II GTNNAC 6 cut(s) 49, 1417, 1697, 1728, 1772, 1863
Hpy8I GTNNAC 6 cut(s) 49, 1417, 1697, 1728, 1772, 1863
Hpy99I CGWCG 3 cut(s) 20, 23, 1864
HpyCH4III ACNGT 6 cut(s) 186, 649, 1976, 2449, 2620, 2678
HpyCH4IV ACGT 4 cut(s) 18, 351, 513, 2635
HpyCH4V TGCA 8 cut(s) 1022, 1181, 1242, 1577, 1934, 1979, 2783, 2804
HpyF10VI GCNNNNNNNGC 5 cut(s) 533, 1117, 1239, 1814, 2651
HpySE526I ACGT 4 cut(s) 18, 351, 513, 2635
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 6 cut(s) 476, 493, 787, 1165, 2080, 2107
HspAI GCGC 3 cut(s) 65, 1645, 1672
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
LmnI GCTCC 7 cut(s) 213, 541, 568, 1988, 2249, 2336, 2344
Lsp1109I GCAGC 5 cut(s) 1006, 1333, 1820, 2673, 2728
LweI GCATC 7 cut(s) 370, 589, 1031, 1513, 1804, 2031, 2632
MaeI CTAG 4 cut(s) 770, 938, 959, 1424
MaeII ACGT 4 cut(s) 18, 351, 513, 2635
MaeIII GTNAC 5 cut(s) 38, 626, 1432, 2268, 2702
MalI GATC 9 cut(s) 558, 699, 957, 1004, 1011, 2073, 2169, 2394, 2510
MboI GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
MfeI CAATTG 3 cut(s) 1182, 1462, 1538
MflI RGATCY 3 cut(s) 1002, 2071, 2392
MhlI GDGCHC 4 cut(s) 1447, 1958, 2246, 2341
MlyI GAGTC 9 cut(s) 601, 716, 988, 1823, 1865, 2314, 2396, 2447, 2540
MmeI TCCRAC 4 cut(s) 280, 631, 1891, 2370
MroNI GCCGGC 1 cut(s) 61
MspA1I CMGCKG 2 cut(s) 1019, 1811
MspI CCGG 4 cut(s) 62, 674, 2247, 2611
MspR9I CCNGG 7 cut(s) 674, 988, 996, 2120, 2334, 2612, 2793
MunI CAATTG 3 cut(s) 1182, 1462, 1538
MvaI CCWGG 5 cut(s) 988, 996, 2120, 2334, 2793
MwoI GCNNNNNNNGC 5 cut(s) 533, 1117, 1239, 1814, 2651
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 2 cut(s) 674, 2612
NcoI CCATGG 1 cut(s) 783
NdeII GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 6 cut(s) 476, 493, 787, 1165, 2080, 2107
NlaIV GGNNCC 6 cut(s) 273, 670, 1445, 1608, 2394, 2581
NmeAIII GCCGAG 1 cut(s) 1811
NmuCI GTSAC 4 cut(s) 38, 626, 1432, 2268
PagI TCATGA 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 164, 950, 1296, 2405
PfoI TCCNGGA 1 cut(s) 2118
PkrI GCNGC 8 cut(s) 1021, 1348, 1611, 1810, 2065, 2573, 2663, 2743
PleI GAGTC 9 cut(s) 600, 715, 987, 1823, 1864, 2313, 2395, 2446, 2539
PpsI GAGTC 9 cut(s) 600, 715, 987, 1823, 1864, 2313, 2395, 2446, 2539
PshAI GACNNNNGTC 1 cut(s) 591
Psp124BI GAGCTC 3 cut(s) 1958, 2246, 2341
Psp1406I AACGTT 2 cut(s) 351, 2635
Psp6I CCWGG 5 cut(s) 986, 994, 2118, 2332, 2791
PspFI CCCAGC 3 cut(s) 67, 246, 287
PspGI CCWGG 5 cut(s) 986, 994, 2118, 2332, 2791
PspN4I GGNNCC 6 cut(s) 273, 670, 1445, 1608, 2394, 2581
PspOMI GGGCCC 1 cut(s) 1443
PstNI CAGNNNCTG 2 cut(s) 1871, 2792
PsuI RGATCY 3 cut(s) 1002, 2071, 2392
PvuII CAGCTG 2 cut(s) 1019, 1811
RsaI GTAC 3 cut(s) 2024, 2680, 2717
RsaNI GTAC 3 cut(s) 2023, 2679, 2716
SacI GAGCTC 3 cut(s) 1958, 2246, 2341
SalI GTCGAC 1 cut(s) 1861
SatI GCNGC 8 cut(s) 1020, 1347, 1610, 1809, 2064, 2572, 2662, 2742
Sau3AI GATC 9 cut(s) 556, 697, 955, 1002, 1009, 2071, 2167, 2392, 2508
ScaI AGTACT 1 cut(s) 2680
SchI GAGTC 9 cut(s) 601, 716, 988, 1823, 1865, 2314, 2396, 2447, 2540
ScrFI CCNGG 7 cut(s) 674, 988, 996, 2120, 2334, 2612, 2793
SduI GDGCHC 4 cut(s) 1447, 1958, 2246, 2341
SfaNI GCATC 7 cut(s) 370, 589, 1031, 1513, 1804, 2031, 2632
SfcI CTRYAG 1 cut(s) 2067
SinI GGWCC 5 cut(s) 4, 272, 2146, 2579, 2687
SmlI CTYRAG 6 cut(s) 1207, 2095, 2300, 2384, 2467, 2711
SmoI CTYRAG 6 cut(s) 1207, 2095, 2300, 2384, 2467, 2711
SsiI CCGC 9 cut(s) 236, 275, 1111, 1213, 1610, 1637, 2063, 2572, 2608
SspI AATATT 2 cut(s) 1533, 2565
SspMI CTAG 4 cut(s) 770, 938, 959, 1424
SstI GAGCTC 3 cut(s) 1958, 2246, 2341
StyD4I CCNGG 7 cut(s) 672, 986, 994, 2118, 2332, 2610, 2791
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 6 cut(s) 186, 649, 1976, 2449, 2620, 2678
TaiI ACGT 4 cut(s) 21, 354, 516, 2638
TaqI TCGA 4 cut(s) 520, 1862, 2308, 2628
TaqII GACCGA 1 cut(s) 1535
TatI WGTACW 1 cut(s) 2678
TauI GCSGC 3 cut(s) 1612, 2066, 2574
TfiI GAWTC 4 cut(s) 164, 950, 1296, 2405
TscAI CASTG 8 cut(s) 1032, 1306, 1491, 1559, 1740, 1981, 2454, 2625
TseFI GTSAC 4 cut(s) 38, 626, 1432, 2268
TseI GCWGC 5 cut(s) 1019, 1346, 1808, 2661, 2741
Tsp45I GTSAC 4 cut(s) 38, 626, 1432, 2268
TspGWI ACGGA 3 cut(s) 935, 1861, 2524
TspRI CASTG 8 cut(s) 1032, 1306, 1491, 1559, 1740, 1981, 2454, 2625
VpaK11BI GGWCC 5 cut(s) 4, 272, 2146, 2579, 2687
XapI RAATTY 5 cut(s) 941, 1169, 2123, 2277, 2516
XcmI CCANNNNNNNNNTGG 2 cut(s) 460, 1181
XmiI GTMKAC 3 cut(s) 1696, 1727, 1862
XspI CTAG 4 cut(s) 770, 938, 959, 1424
ZraI GACGTC 1 cut(s) 19
ZrmI AGTACT 1 cut(s) 2680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.