Rw3G013720

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002940
Physical Location & Seq
Forward (+)
13551792 .. 13554480
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013720.1

Sequence Viewer

Length: 1692 bp
ATGGATGCGAACATGTTTAGTGATGTGAATGGCCAATATGATCCAAAACCGGAATTCCATGTTTATGATATAGTATGGGGGAAAGTAAGGGGTTATCCCTGGTGGCCTGGACGGATATGTGATCCTTCAACTTCATCGAAAGAGGCAAAGATGCTTTTCAGAAGGGGTAGTTATTTGATAGATTATTTTGGGGATCACTCATTTACTTGGAATGAAGCACCGCATATTAAGCCATTTCTGGATTATTTCTCGCAAATGGAGAAGCAGAGCAACAAGGACGAATTCCAAAATGCCATTGCTTGTGCTCTGGACGAATTCTTAAGACGGGTAGAGTTGGGGGTGGTCTGTTCTTGCATATCTGGAGAGGTATATCCAAAACTCAAAAATCAGATAATTATTAGTGATGGAGTCAGGCAAGAAGAAGAAGAAGAAGAAGACAGTGGAAATGCTGGTGACGGTTCCCTTACCGCCGTCTCTTTTGAACCTCAGAAACTCATTATGCTTCTAAAGGAGTTTGCTCAGTTTTCATATGGAAAAGCTGATCAACTAGAACTTTCAACATCAGTGATCCAATTATCAGCTTTCTATTGTTGGAAGAGTTCTCAGCTGTCGCTGCCAACGGAGAATGATGCCTATATATCAGTATTGGGGGAGAAGACTCAATTCAATGTATTAGATCACAATTCACTTCCAGTAACTACAGATGAGAAGCTGGTGCAGAAATCTAAAAGTAAAGTGTGGCTGATGAAGAAGCAACGCACAATTCACACCCGCAGGAATAAAGTCCTGGTTCCGCCCCTGTGTTTGTCTATTCCAATGACTGATAATGCAGCAGAAGGGGAAGCTAGTGGTTTGGTTTCTTTACCTTCCATGAGTGAGAATACATCAGAAGGGGAAACTAACAGTAAATTGATTTTGCAATCTTCTAATGAGAATCCAGGGGAAGTTGATGGTATAGCGGATGAAGATGTGACTGCAGTCAGTGAAGCTGCACTTCCAGTAACTAAGGATGAGAAATTGGTGCGGAAATCAGAAAATAAAGATAACTCATTGCACAAGTGTAAGCACATGTCTGAGTGCGGTATGAATTCGAGTGAAAAAGAGAAAACATCATCAGATTTAAAGGCTCATAAGTGTTTGTCTACTCCAATTTGTCAGAATGGATCAGAAGGGAAAGCAGGCAGTAAATTGACTTTACAGTCCTGTGTAAAGCGCAAGGCAGTTGATGTTATAGCTGATGACTTTGTGCTGAAACATAGGAAAAGTGATTCATCAACGAGGACTAAAGATGCACTTCCGCTAACTAAGGATGAGATGTTGGTGAGGAAATCAGAAAGTAAAAATAACTCGATGCATAAGTGCAAGAACATATGTGGAGATATGATGAATCCAAGTGAAGAACAGAAAAGTTTATCGGATTTAAATGGTGGGAAGTGTTTGTCTCCAGCAAGTGAGTATGGATTTCCTTCTGATCGTACATTGACTTTGCACCCCTCTAGTAGTAATATACGAAAGGAAGTCGATCCTATTCCTATAGCTGATGACTATGCACTAAAAGATTGGGAAATCAATTTATCAGCAGGGGATGTTGAGCAGTCTTTGGAGTTGCAGATTTTATTAACTTGTTTCTACAGAGGGAGCACATTTTTGGAGCCGGAGAAAGGATCTGTCTGGTTGAATGAAACTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

563

Amino Acids

62.85

Weight (kDa)

5.25

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 23 - 105 1.1e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1198
AccI GTMKAC 1 cut(s) 1142
AciI CCGC 8 cut(s) 221, 468, 772, 794, 959, 1024, 1080, 1298
AclWI GGATC 7 cut(s) 35, 116, 201, 562, 1171, 1517, 1672
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 4 cut(s) 53, 281, 314, 1087
AfaI GTAC 1 cut(s) 1477
AfiI CCNNNNNNNGG 1 cut(s) 1661
AflII CTTAAG 1 cut(s) 319
AflIII ACRYGT 2 cut(s) 12, 1068
AgsI TTSAA 5 cut(s) 129, 482, 558, 667, 1678
AjnI CCWGG 4 cut(s) 98, 106, 786, 937
AluBI AGCT 8 cut(s) 539, 581, 607, 712, 845, 989, 1235, 1538
AluI AGCT 8 cut(s) 539, 581, 607, 712, 845, 989, 1235, 1538
Alw21I GWGCWC 2 cut(s) 307, 1643
Alw26I GTCTC 2 cut(s) 478, 1446
AlwI GGATC 7 cut(s) 35, 116, 201, 562, 1171, 1517, 1672
AoxI GGCC 2 cut(s) 31, 104
ApeKI GCWGC 3 cut(s) 613, 830, 989
ApoI RAATTY 4 cut(s) 53, 281, 314, 1087
AspLEI GCGC 1 cut(s) 1215
AsuHPI GGTGA 2 cut(s) 464, 1333
BalI TGGCCA 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 441, 662
Bbv12I GWGCWC 2 cut(s) 307, 1643
BbvI GCAGC 3 cut(s) 600, 842, 976
BccI CCATC 2 cut(s) 398, 944
BceAI ACGGC 1 cut(s) 455
BciT130I CCWGG 4 cut(s) 100, 108, 788, 939
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 2 cut(s) 478, 1446
BfaI CTAG 3 cut(s) 548, 846, 1497
BfmI CTRYAG 4 cut(s) 699, 975, 1533, 1630
BfrI CTTAAG 1 cut(s) 319
BisI GCNGC 3 cut(s) 614, 831, 990
BlsI GCNGC 3 cut(s) 615, 832, 991
Bme1390I CCNGG 4 cut(s) 100, 108, 788, 939
BmiI GGNNCC 3 cut(s) 460, 792, 1653
BmrFI CCNGG 4 cut(s) 100, 108, 788, 939
BmsI GCATC 4 cut(s) 141, 619, 1279, 1341
BoxI GACNNNNGTC 1 cut(s) 977
BpiI GAAGAC 2 cut(s) 441, 662
BpmI CTGGAG 2 cut(s) 381, 1428
BsaJI CCNNGG 2 cut(s) 98, 938
BsaWI WCCGGW 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 1661
Bse1I ACTGG 2 cut(s) 692, 998
Bse3DI GCAATG 2 cut(s) 294, 1049
BseBI CCWGG 4 cut(s) 100, 108, 788, 939
BseDI CCNNGG 2 cut(s) 98, 938
BseGI GGATG 5 cut(s) 10, 967, 1015, 1315, 1591
BseLI CCNNNNNNNGG 1 cut(s) 1661
BseMI GCAATG 2 cut(s) 294, 1049
BseMII CTCAG 4 cut(s) 500, 533, 617, 1065
BseNI ACTGG 2 cut(s) 692, 998
BseXI GCAGC 3 cut(s) 600, 842, 976
BsgI GTGCAG 2 cut(s) 737, 975
BshFI GGCC 2 cut(s) 33, 106
BsiHKAI GWGCWC 2 cut(s) 307, 1643
BsiSI CCGG 2 cut(s) 50, 1655
BslI CCNNNNNNNGG 1 cut(s) 1661
BsmAI GTCTC 2 cut(s) 478, 1446
BsmBI CGTCTC 1 cut(s) 478
BsnI GGCC 2 cut(s) 33, 106
Bsp1286I GDGCHC 2 cut(s) 307, 1643
BspACI CCGC 8 cut(s) 221, 468, 772, 794, 959, 1024, 1080, 1298
BspANI GGCC 2 cut(s) 33, 106
BspCNI CTCAG 4 cut(s) 499, 532, 616, 1066
BspLI GGNNCC 3 cut(s) 460, 792, 1653
BspMAI CTGCAG 1 cut(s) 979
BspPI GGATC 7 cut(s) 35, 116, 201, 562, 1171, 1517, 1672
BspTI CTTAAG 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 294, 1049
BsrI ACTGG 2 cut(s) 692, 998
BssECI CCNNGG 2 cut(s) 98, 938
Bst2UI CCWGG 4 cut(s) 100, 108, 788, 939
Bst4CI ACNGT 5 cut(s) 440, 458, 905, 1200, 1687
Bst6I CTCTTC 1 cut(s) 590
BstAFI CTTAAG 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 1180
BstDEI CTNAG 6 cut(s) 486, 519, 603, 1005, 1074, 1305
BstF5I GGATG 5 cut(s) 10, 967, 1015, 1315, 1591
BstHHI GCGC 1 cut(s) 1215
BstMAI GTCTC 2 cut(s) 478, 1446
BstMWI GCNNNNNNNGC 2 cut(s) 229, 613
BstNI CCWGG 4 cut(s) 100, 108, 788, 939
BstNSI RCATGY 2 cut(s) 16, 1072
BstPAI GACNNNNGTC 1 cut(s) 977
BstSCI CCNGG 4 cut(s) 98, 106, 786, 937
BstSFI CTRYAG 4 cut(s) 699, 975, 1533, 1630
BstV1I GCAGC 3 cut(s) 600, 842, 976
BstV2I GAAGAC 2 cut(s) 441, 662
BstX2I RGATCY 1 cut(s) 1664
BstYI RGATCY 1 cut(s) 1664
BsuRI GGCC 2 cut(s) 33, 106
BtsCI GGATG 5 cut(s) 10, 967, 1015, 1315, 1591
BtsIMutI CAGTG 3 cut(s) 445, 570, 988
Cac8I GCNNGC 1 cut(s) 1180
CfoI GCGC 1 cut(s) 1215
Csp6I GTAC 1 cut(s) 1476
CviAII CATG 4 cut(s) 13, 59, 871, 1069
CviQI GTAC 1 cut(s) 1476
DdeI CTNAG 6 cut(s) 486, 519, 603, 1005, 1074, 1305
DraI TTTAAA 2 cut(s) 1122, 1422
DrdI GACNNNNNNGTC 1 cut(s) 1198
DseDI GACNNNNNNGTC 1 cut(s) 1198
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
EciI GGCGGA 1 cut(s) 783
EcoRI GAATTC 4 cut(s) 53, 281, 314, 1087
EcoRII CCWGG 4 cut(s) 98, 106, 786, 937
EcoT22I ATGCAT 1 cut(s) 1356
Esp3I CGTCTC 1 cut(s) 478
FaeI CATG 4 cut(s) 16, 62, 874, 1072
FalI AAGNNNNNCTT 4 cut(s) 978, 1010, 1278, 1310
FatI CATG 4 cut(s) 12, 58, 870, 1068
FauI CCCGC 1 cut(s) 779
FauNDI CATATG 2 cut(s) 529, 1370
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 1 cut(s) 1142
Fnu4HI GCNGC 3 cut(s) 614, 831, 990
FokI GGATG 5 cut(s) 17, 974, 1022, 1322, 1598
Fsp4HI GCNGC 3 cut(s) 614, 831, 990
FspBI CTAG 3 cut(s) 548, 846, 1497
GlaI GCGC 1 cut(s) 1214
GluI GCNGC 3 cut(s) 614, 831, 990
GsuI CTGGAG 2 cut(s) 381, 1428
HaeIII GGCC 2 cut(s) 33, 106
HapII CCGG 2 cut(s) 50, 1655
HhaI GCGC 1 cut(s) 1215
Hin1II CATG 4 cut(s) 16, 62, 874, 1072
Hin6I GCGC 1 cut(s) 1213
HinP1I GCGC 1 cut(s) 1213
HinfI GANTC 5 cut(s) 408, 658, 934, 1268, 1387
HpaII CCGG 2 cut(s) 50, 1655
HphI GGTGA 2 cut(s) 464, 1333
Hpy166II GTNNAC 1 cut(s) 1143
Hpy188III TCNNGA 3 cut(s) 239, 308, 360
Hpy8I GTNNAC 1 cut(s) 1143
HpyAV CCTTC 7 cut(s) 135, 156, 830, 876, 884, 1163, 1476
HpyCH4III ACNGT 5 cut(s) 440, 458, 905, 1200, 1687
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 613
HpyF3I CTNAG 6 cut(s) 486, 519, 603, 1005, 1074, 1305
Hsp92II CATG 4 cut(s) 16, 62, 874, 1072
HspAI GCGC 1 cut(s) 1213
Ksp22I TGATCA 1 cut(s) 541
LmnI GCTCC 2 cut(s) 1638, 1651
Lsp1109I GCAGC 3 cut(s) 600, 842, 976
LweI GCATC 4 cut(s) 141, 619, 1279, 1341
MaeI CTAG 3 cut(s) 548, 846, 1497
MaeIII GTNAC 4 cut(s) 452, 694, 970, 1000
MflI RGATCY 1 cut(s) 1664
MhlI GDGCHC 2 cut(s) 307, 1643
MlsI TGGCCA 1 cut(s) 33
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 2 cut(s) 417, 652
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 7 cut(s) 136, 358, 495, 1272, 1317, 1504, 1628
Mox20I TGGCCA 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 1356
MscI TGGCCA 1 cut(s) 33
MseI TTAA 5 cut(s) 228, 320, 1121, 1421, 1619
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 607
MspCI CTTAAG 1 cut(s) 319
MspI CCGG 2 cut(s) 50, 1655
MspR9I CCNGG 4 cut(s) 100, 108, 788, 939
MvaI CCWGG 4 cut(s) 100, 108, 788, 939
MwoI GCNNNNNNNGC 2 cut(s) 229, 613
NdeI CATATG 2 cut(s) 529, 1370
NlaIII CATG 4 cut(s) 16, 62, 874, 1072
NlaIV GGNNCC 3 cut(s) 460, 792, 1653
NmuCI GTSAC 2 cut(s) 452, 970
NsiI ATGCAT 1 cut(s) 1356
NspI RCATGY 2 cut(s) 16, 1072
PciI ACATGT 2 cut(s) 12, 1068
PfeI GAWTC 3 cut(s) 934, 1268, 1387
PkrI GCNGC 3 cut(s) 615, 832, 991
PleI GAGTC 2 cut(s) 416, 652
PpsI GAGTC 2 cut(s) 416, 652
PscI ACATGT 2 cut(s) 12, 1068
PshAI GACNNNNGTC 1 cut(s) 977
Psp6I CCWGG 4 cut(s) 98, 106, 786, 937
PspGI CCWGG 4 cut(s) 98, 106, 786, 937
PspN4I GGNNCC 3 cut(s) 460, 792, 1653
PstI CTGCAG 1 cut(s) 979
PsuI RGATCY 1 cut(s) 1664
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 1 cut(s) 1477
RsaNI GTAC 1 cut(s) 1476
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 5 cut(s) 228, 320, 1121, 1421, 1619
SatI GCNGC 3 cut(s) 614, 831, 990
SchI GAGTC 2 cut(s) 417, 652
ScrFI CCNGG 4 cut(s) 100, 108, 788, 939
SduI GDGCHC 2 cut(s) 307, 1643
SfaNI GCATC 4 cut(s) 141, 619, 1279, 1341
SfcI CTRYAG 4 cut(s) 699, 975, 1533, 1630
SmiI ATTTAAAT 1 cut(s) 1422
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
SsiI CCGC 8 cut(s) 221, 468, 772, 794, 959, 1024, 1080, 1298
SspMI CTAG 3 cut(s) 548, 846, 1497
StyD4I CCNGG 4 cut(s) 98, 106, 786, 937
SwaI ATTTAAAT 1 cut(s) 1422
TaaI ACNGT 5 cut(s) 440, 458, 905, 1200, 1687
TaqI TCGA 4 cut(s) 137, 1091, 1349, 1521
TfiI GAWTC 3 cut(s) 934, 1268, 1387
Tru1I TTAA 5 cut(s) 228, 320, 1121, 1421, 1619
Tru9I TTAA 5 cut(s) 228, 320, 1121, 1421, 1619
TscAI CASTG 3 cut(s) 445, 570, 988
TseFI GTSAC 2 cut(s) 452, 970
TseI GCWGC 3 cut(s) 613, 830, 989
Tsp45I GTSAC 2 cut(s) 452, 970
TspDTI ATGAA 8 cut(s) 123, 228, 516, 761, 978, 1100, 1260, 1400
TspGWI ACGGA 2 cut(s) 127, 635
TspRI CASTG 3 cut(s) 445, 570, 988
Vha464I CTTAAG 1 cut(s) 319
XapI RAATTY 4 cut(s) 53, 281, 314, 1087
XceI RCATGY 2 cut(s) 16, 1072
XmiI GTMKAC 1 cut(s) 1142
XspI CTAG 3 cut(s) 548, 846, 1497
Zsp2I ATGCAT 1 cut(s) 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.