Rh3DG166700

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002940
Physical Location & Seq
Forward (+)
14515502 .. 14517453
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG166700.1

Sequence Viewer

Length: 1596 bp
ATGGATACTGACTGTGGAGCTGAATTTGGTGAATGGTTGGTTTTTGATGCCAAGGATAGTTGTAAGAATGTTGAAAGTTCGTTAATTTCGGATAAAGATAGAGTAAAAGATGAGACTTTGAGGGAAAGGATGGAAGGTAAAGTTGTGAGTGCTCTGGGAGTGGAATTTACTAATAACAGTGAGTCTTTGGATTATCAAGACTTCAATGCTAGCGGCTGTTTTAAGACGGTAGCTTGTGATGGACTCGAAAATGAGGCCTTGGAAGTTAATGTGGAAGAAGAGAGTAATCGAAATGAATTGACGCCGATGAGGGTCCCTGTGAGTACTGAATGCGAGGTTGATTTGCTCAGTGTTGCTGATTTTATCCGGTGTTCGAGAATGGACGGGAATGTGTCAAGTGGTGTGAATGGAAAATCTTGTTCTTCCAAGCTAGAATTTGAAGTTTCTGATCTAGTATGGGGCAAAGTAAGGACTCATCCATGGTGGCCCGGGCAGATTTGTGATCCTTCGGCTGCGTCAGAACTGGCAAAGAAGTGTTTTAAGAGGGGTAGTTATCTGATAGCTTACTTTGGGGATCAGACATTTGCTTGGAATGAAGCACCAAAGATTAAGCCCTTTCTGGAGCACTGCTCAAAAATGGAGAAGCAGAGTGATATGGAAGAATTCCAAAATGCAGTTGGTTGTGCGTTGGACGAAGTGTCGAGAAGGGTAGAGTTTGGGCTGGCCTGTTCTTGCATATCCAAACGAGAATATGCAAAGCTCAAAACTCAGAAGATTATTAATGCTGGAATCAGGAAAGAAGCAGCAAATAGAAGAGATGGCGGTGACAGTTCTCTTAGTGCCGCTTTTTTTGAACCTACGAAAGTCATTAAGTTTATAAAAAAGTTGGCTCAGTTGCCATATGGCAAAGCTGACAGACTAGAACTTGTAACATCTCTAGCCCAGCTGTCAGCTTTCTGGCGTTGGAAGAGTTATGTGCCTCTGCCAGAGGAGAATGATACTGACATTTTCCTATTGGGAGAGAAGAACCACTGCAATGAAGTAAGCGATGAAGCACTCTCAGTACTTGAGAATGAGAAGTTGGTGCAAAAATCAGATAGTAAAGAGAACTCATTACCTGAGTGCAAGGACATATCAGATTCAAAAGCTCAGAGGTGTTTGTTTCCTATGAGTGAGAATGGATCAGAAAGGAAAGCTGGCAGTGAATTGATTTTGCAACCTTGCAACAAAAGAAGGGGTCTTGATGCTATAGCTGATGAAGATGTACTCCCAGTAATCAAAGATAACTCAATGCATAAGTGTTGGCACATATCTGAACATAGTATGAATCTGAGTGAAGAAGAGCAAAGCTTGTCAGATTTGGAGGGTACGAAGTGTTTGTCTACTCCAATGAGTGAGAATGGATTAGAAGAGAAAGGTGACGGTAAATTGATTTCACAACCTTCTGTTAAGAAACGAAAGGAAGTTGATGCTATGGCTGATGATGCTGCTGTGAAACATAGGAAAAGTGATTCATCACAAGGTATTGGTAGCAATGCGAACTGTCAGTTGTTTGCAGTTGAAGACTTTTTTAACCTGTTTTTACAGCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

531

Amino Acids

59.24

Weight (kDa)

4.94

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 150 - 232 1.5e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 878
AccI GTMKAC 1 cut(s) 1382
AciI CCGC 3 cut(s) 213, 822, 843
AclWI GGATC 3 cut(s) 497, 582, 1189
AcsI RAATTY 4 cut(s) 23, 164, 434, 662
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 4 cut(s) 325, 1065, 1266, 1369
AgsI TTSAA 6 cut(s) 74, 205, 440, 854, 1143, 1562
AhdI GACNNNNNGTC 1 cut(s) 697
AjuI GAANNNNNNNTTGG 2 cut(s) 1064, 1096
Alw21I GWGCWC 2 cut(s) 154, 627
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 3 cut(s) 497, 582, 1189
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 3 cut(s) 255, 485, 723
ApeKI GCWGC 3 cut(s) 512, 803, 1487
ApoI RAATTY 4 cut(s) 23, 164, 434, 662
ArsI GACNNNNNNTTYG 2 cut(s) 1364, 1396
AseI ATTAAT 1 cut(s) 780
AspS9I GGNCC 2 cut(s) 313, 486
AsuC2I CCSGG 2 cut(s) 489, 490
AsuHPI GGTGA 3 cut(s) 41, 836, 1430
AsuNHI GCTAGC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 1569
Bbv12I GWGCWC 2 cut(s) 154, 627
BbvI GCAGC 3 cut(s) 499, 815, 1474
BccI CCATC 3 cut(s) 124, 233, 812
BcnI CCSGG 2 cut(s) 489, 490
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 5 cut(s) 210, 431, 452, 920, 938
BfmI CTRYAG 1 cut(s) 1248
BisI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
BlsI GCNGC 5 cut(s) 215, 514, 805, 844, 1489
BmcAI AGTACT 2 cut(s) 325, 1065
Bme1390I CCNGG 2 cut(s) 489, 490
Bme18I GGWCC 1 cut(s) 313
BmeRI GACNNNNNGTC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 2 cut(s) 313, 486
BmiI GGNNCC 2 cut(s) 314, 315
BmrFI CCNGG 2 cut(s) 489, 490
BmrI ACTGGG 1 cut(s) 1265
BmsI GCATC 4 cut(s) 37, 1234, 1459, 1474
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 1265
BpiI GAAGAC 1 cut(s) 1569
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 1 cut(s) 641
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 2 cut(s) 489, 490
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 4 cut(s) 51, 258, 479, 488
BsaWI WCCGGW 1 cut(s) 366
Bse1I ACTGG 2 cut(s) 528, 1271
Bse3DI GCAATG 2 cut(s) 1042, 1540
BseDI CCNNGG 4 cut(s) 51, 258, 479, 488
BseGI GGATG 2 cut(s) 135, 475
BseMI GCAATG 2 cut(s) 1042, 1540
BseMII CTCAG 7 cut(s) 361, 782, 905, 1074, 1110, 1163, 1322
BseNI ACTGG 2 cut(s) 528, 1271
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 3 cut(s) 499, 815, 1474
BseYI CCCAGC 1 cut(s) 942
BshFI GGCC 3 cut(s) 257, 487, 725
BsiHKAI GWGCWC 2 cut(s) 154, 627
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 2 cut(s) 367, 489
BslFI GGGAC 1 cut(s) 299
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 3 cut(s) 257, 487, 725
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 154, 627
Bsp143I GATC 4 cut(s) 448, 502, 574, 1181
Bsp19I CCATGG 1 cut(s) 479
BspACI CCGC 3 cut(s) 213, 822, 843
BspANI GGCC 3 cut(s) 257, 487, 725
BspCNI CTCAG 7 cut(s) 360, 781, 904, 1073, 1111, 1162, 1323
BspLI GGNNCC 2 cut(s) 314, 315
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 497, 582, 1189
BspQI GCTCTTC 1 cut(s) 1335
BsrDI GCAATG 2 cut(s) 1042, 1540
BsrI ACTGG 2 cut(s) 528, 1271
BssECI CCNNGG 4 cut(s) 51, 258, 479, 488
BssMI GATC 4 cut(s) 448, 502, 574, 1181
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 3 cut(s) 51, 258, 479
Bst4CI ACNGT 6 cut(s) 14, 179, 229, 830, 1424, 1544
Bst6I CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
BstACI GRCGYC 1 cut(s) 302
BstC8I GCNNGC 3 cut(s) 211, 723, 1198
BstDEI CTNAG 8 cut(s) 347, 768, 836, 891, 1060, 1119, 1149, 1331
BstDSI CCRYGG 1 cut(s) 479
BstF5I GGATG 2 cut(s) 135, 475
BstKTI GATC 4 cut(s) 451, 505, 577, 1184
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 4 cut(s) 448, 502, 574, 1181
BstMWI GCNNNNNNNGC 1 cut(s) 1484
BstSCI CCNGG 2 cut(s) 487, 488
BstSFI CTRYAG 1 cut(s) 1248
BstV1I GCAGC 3 cut(s) 499, 815, 1474
BstV2I GAAGAC 1 cut(s) 1569
BsuRI GGCC 3 cut(s) 257, 487, 725
BtgI CCRYGG 1 cut(s) 479
BtgZI GCGATG 1 cut(s) 1062
BtsCI GGATG 2 cut(s) 135, 475
BtsI GCAGTG 3 cut(s) 625, 1030, 1207
BtsIMutI CAGTG 5 cut(s) 184, 355, 625, 1030, 1207
Cac8I GCNNGC 3 cut(s) 211, 723, 1198
Cfr13I GGNCC 2 cut(s) 313, 486
Cfr9I CCCGGG 1 cut(s) 488
CseI GACGC 2 cut(s) 310, 504
Csp6I GTAC 4 cut(s) 324, 1064, 1265, 1368
CviAII CATG 1 cut(s) 480
CviQI GTAC 4 cut(s) 324, 1064, 1265, 1368
DdeI CTNAG 8 cut(s) 347, 768, 836, 891, 1060, 1119, 1149, 1331
DpnI GATC 4 cut(s) 450, 504, 576, 1183
DpnII GATC 4 cut(s) 448, 502, 574, 1181
DriI GACNNNNNGTC 1 cut(s) 697
Eam1104I CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
Eam1105I GACNNNNNGTC 1 cut(s) 697
EarI CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
Eco130I CCWWGG 3 cut(s) 51, 258, 479
Eco147I AGGCCT 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 662
EcoT14I CCWWGG 3 cut(s) 51, 258, 479
EcoT22I ATGCAT 1 cut(s) 1296
ErhI CCWWGG 3 cut(s) 51, 258, 479
FaeI CATG 1 cut(s) 483
FaqI GGGAC 1 cut(s) 299
FatI CATG 1 cut(s) 479
FauNDI CATATG 1 cut(s) 901
FblI GTMKAC 1 cut(s) 1382
Fnu4HI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
FokI GGATG 2 cut(s) 142, 462
Fsp4HI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
FspBI CTAG 5 cut(s) 210, 431, 452, 920, 938
GluI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 1 cut(s) 641
HaeIII GGCC 3 cut(s) 257, 487, 725
HapII CCGG 2 cut(s) 367, 489
HgaI GACGC 2 cut(s) 310, 504
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 1 cut(s) 483
HindIII AAGCTT 1 cut(s) 1348
HinfI GANTC 7 cut(s) 182, 243, 472, 789, 1139, 1327, 1511
HpaII CCGG 2 cut(s) 367, 489
HphI GGTGA 3 cut(s) 41, 836, 1430
Hpy166II GTNNAC 1 cut(s) 1383
Hpy188III TCNNGA 6 cut(s) 197, 375, 620, 702, 793, 1241
Hpy8I GTNNAC 1 cut(s) 1383
HpyAV CCTTC 5 cut(s) 128, 516, 699, 1227, 1452
HpyCH4III ACNGT 6 cut(s) 14, 179, 229, 830, 1424, 1544
HpyF10VI GCNNNNNNNGC 1 cut(s) 1484
HpyF3I CTNAG 8 cut(s) 347, 768, 836, 891, 1060, 1119, 1149, 1331
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 1 cut(s) 483
KflI GGGWCCC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 448, 502, 574, 1181
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 2 cut(s) 17, 622
Lsp1109I GCAGC 3 cut(s) 499, 815, 1474
LweI GCATC 4 cut(s) 37, 1234, 1459, 1474
MaeI CTAG 5 cut(s) 210, 431, 452, 920, 938
MaeIII GTNAC 3 cut(s) 824, 928, 1418
MalI GATC 4 cut(s) 450, 504, 576, 1183
MboI GATC 4 cut(s) 448, 502, 574, 1181
MhlI GDGCHC 2 cut(s) 154, 627
MluCI AATT 8 cut(s) 23, 84, 164, 296, 434, 662, 1205, 1427
MlyI GAGTC 3 cut(s) 191, 237, 466
MmeI TCCRAC 2 cut(s) 669, 944
MnlI CCTC 9 cut(s) 114, 247, 303, 328, 537, 982, 990, 1146, 1357
Mph1103I ATGCAT 1 cut(s) 1296
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 1 cut(s) 946
MspI CCGG 2 cut(s) 367, 489
MspR9I CCNGG 2 cut(s) 489, 490
Mva1269I GAATGC 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 1484
NciI CCSGG 2 cut(s) 489, 490
NcoI CCATGG 1 cut(s) 479
NdeI CATATG 1 cut(s) 901
NdeII GATC 4 cut(s) 448, 502, 574, 1181
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 1 cut(s) 483
NlaIV GGNNCC 2 cut(s) 314, 315
NmuCI GTSAC 2 cut(s) 824, 1418
NsiI ATGCAT 1 cut(s) 1296
PceI AGGCCT 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 1335
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 789, 1139, 1327, 1511
PkrI GCNGC 5 cut(s) 215, 514, 805, 844, 1489
PleI GAGTC 3 cut(s) 190, 237, 466
PpsI GAGTC 3 cut(s) 190, 237, 466
PpuMI RGGWCCY 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 780
PsiI TTATAA 1 cut(s) 878
Psp5II RGGWCCY 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 942
PspN4I GGNNCC 2 cut(s) 314, 315
PspPI GGNCC 2 cut(s) 313, 486
PspPPI RGGWCCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 4 cut(s) 325, 1065, 1266, 1369
RsaNI GTAC 4 cut(s) 324, 1064, 1265, 1368
RseI CAYNNNNRTG 1 cut(s) 1035
SapI GCTCTTC 1 cut(s) 1335
SatI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
Sau3AI GATC 4 cut(s) 448, 502, 574, 1181
Sau96I GGNCC 2 cut(s) 313, 486
ScaI AGTACT 2 cut(s) 325, 1065
SchI GAGTC 3 cut(s) 191, 237, 466
ScrFI CCNGG 2 cut(s) 489, 490
SduI GDGCHC 2 cut(s) 154, 627
SfaNI GCATC 4 cut(s) 37, 1234, 1459, 1474
SfcI CTRYAG 1 cut(s) 1248
SinI GGWCC 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 1035
SmlI CTYRAG 1 cut(s) 1067
SmoI CTYRAG 1 cut(s) 1067
SrfI GCCCGGGC 1 cut(s) 490
Sse9I AATT 8 cut(s) 23, 84, 164, 296, 434, 662, 1205, 1427
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 3 cut(s) 213, 822, 843
SspMI CTAG 5 cut(s) 210, 431, 452, 920, 938
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 487, 488
StyI CCWWGG 3 cut(s) 51, 258, 479
TaaI ACNGT 6 cut(s) 14, 179, 229, 830, 1424, 1544
TaqI TCGA 4 cut(s) 246, 289, 374, 701
TasI AATT 8 cut(s) 23, 84, 164, 296, 434, 662, 1205, 1427
TatI WGTACW 3 cut(s) 323, 1063, 1264
TauI GCSGC 2 cut(s) 216, 845
TfiI GAWTC 4 cut(s) 789, 1139, 1327, 1511
TscAI CASTG 5 cut(s) 184, 355, 632, 1037, 1207
TseFI GTSAC 2 cut(s) 824, 1418
TseI GCWGC 3 cut(s) 512, 803, 1487
Tsp45I GTSAC 2 cut(s) 824, 1418
TspDTI ATGAA 7 cut(s) 309, 609, 1053, 1065, 1272, 1340, 1503
TspMI CCCGGG 1 cut(s) 488
TspRI CASTG 5 cut(s) 184, 355, 632, 1037, 1207
VpaK11BI GGWCC 1 cut(s) 313
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 4 cut(s) 23, 164, 434, 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 1382
XspI CTAG 5 cut(s) 210, 431, 452, 920, 938
ZrmI AGTACT 2 cut(s) 325, 1065
Zsp2I ATGCAT 1 cut(s) 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.