MD12G1124400.v1.1

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002940
Physical Location & Seq
Reverse (-)
19931316 .. 19934669
3354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1124400.v1.1.491

Sequence Viewer

Length: 2817 bp
ATGGACCTGAACGCCGACGTCGTTTTGGCTGACCCGAGTGACGGGGTTCACCACTCTGAGCCGGCGCTGGGAGTGGTGGGCGAAGTGAATGAAATGGCTTCTGTGGGAGGTGGAGTGAATGGGGGTGTAGAGGAGAAAGGTGAAGTGGTGGATGGAGGTGATGATTCTGAAGTGAAATTAGGACTTTTAGATGGGGATGTTGAGGGGAAAGGGAGCGTTGTTTTGTCAGAGGTAGCGGTTAATGGCGGGGAAGGTAGAGAGGGCTTGGAGGGTCCGCAATTTGGTGCTGGGGGTGTGGTTGGAGAAAATGTTAATGGGTTGGTTTTGAAATCTGAGGACAGTTTGAGGAACCTTGAAATTGGAAGGGGTAGAGGGGAGGATGCGACTTTAGGGGAATGCACAAAAGATGAAGTTGTGGGTTCTGAGGATGGAGATTTGGTGAGTGTTAAGGCGAAGCGGAATGTGGCAGTTCATGGAAGGGATGACATGACGATAGATGAGAAAGAAGAGAACGTTGTTCGAGTAGCTGATATAGCTCCTGCTGAAATAGAAATGGATCAAAAGGTGGAGCAGGGCCAAGTTGAAGACCAGAGTCTTGATGCTTCCAGTTCTATGCATGGCAATGGTGACGTTGAGTATTTGGAAACTGTTGGACAAATAACAGAAGGCACCCGGGCTGTGGAAGTGAAAGTTGTTGATCATAGTGAGTCTTTGGATAAAAAAGACCTGAATGTAAGCACTCAGTCTAAGATGGAAACCTGTGATGGACTAGAAAATCAGCCCATGGAAGTTAATGTGGAAGCACAGACAAATGAGAACAAATTGACCCATGATGTTTCAGAGAATGGGGTTCGGCAGAATACTGAAAATGGAAAATCTACTTCCTCAGATGGGAATGTGTCAAGTGATGAAAATGGAAAGTCTGCTTCCACAGAACTAGAATTCCGTGATTCTGATCTAGTATGGGGAAAGGTAAGGAGTCATCCCTGGTGGCCCGGGCAGATCTGTGATCCTTCAGCTGCATCAGTGAAGGCAAAGAAGTATTTTAAAAGAGGCACTTATCTAATAATGTATTATTGGGATAATACATTTGCTTGGAATGAAGCAATGCGGATTAAGCCCTTCTTTGAGAACTTTTCACAAATGGAGAAACAAAGCGACATGGAAGAATTCCAAAATGCAATTGGTTGTGCTTTGGATGAAGTCTCAAGGCGGGTAGAATTAGGGCTGAGCTGTTCTTGCATATCCAAAGAAGTATATGAAAAACTCAAAACTCAGATAATTGAAAATGCTGGAATCAGTGAAGAAGCAAGTAGAAGAGATGGTGGTGACAAGTCTTTAAGTGCTGCTTCTTTTGAACCTGTGAAACTCATTAGGTTTGTAAAGAAGTTGGCTCAGTTTCCGTATAGAAAAGTTGACAGACTAGAACTTGTAACATCAAGGGCCCAATTATCAGCCTTCAATCGCTGGAAAGGTTGTCCTCCACTGCCAGAGTTCAATATGCTTGGTGGGTTGCTGACCGATGCTGACATATTACTATTGGGAGAGAAGCAGTGTGCTGAAGCAAGTGAAAATGCACTTCCAGTAATTAAGGATGAGGATTTGGGGCCGAAATCAAAAAGTAAAGATAACTTACCGCAGAAGCGCAAGAACATGTCTGGAGATAGTACGCGCCCGAGTAAAAAAGAGAAAAGCATACCAGATGTAGTGGCTGAGAAGTATTTTTCTACTCCAGTGAGTGAGAAAGGATCGGAAGGGAAAGCTGGCAGTAAACTGATTTCACAATCTTCCAGTAAGAAACGAAAGGCAGGTGATGCTACATCTGATGACTCGGCAGTAAAGCAACGGAAAAGTGAGTTGTCAACAAGGGCTGATAGTAATTCTATACAAAATAAACCGACTTTTAAAGTTGGAGATAGGATACGCAGGGTTGCAAGCCAACTAAGTGGATTGAGCCCAATACTGAAGAGTTACAATGCAACATCTGGAGAAGTTGAAGTTGAGGATAAAGGCAAAAGAAATGAGTACCCATCTCCTGATAAGATGCTGTCTCAGGTCTGCTTGGCTGCTATAGATCCCATGAACGGATACAGCTTCTTGAGTTCCATGATTACTTGCTTCCGGGAATTTAGGAATACTATATGCTTGGACCATTCCAATCCTGCGGAGGACCAAATGTCTCTGGAACAAATGTTTGGTGGTAAGCTGGTTAAGAAATCAATCAGGAGTGGGAAGAAATCAATGAGCTCCGGCATTACTGAAAAATCCAAGTCACAAGAAATTCCTCCTGAGCAAGCATTACCCAAGAGTCGAAATGAAAATGAAGGGTTGGTGCCTGGAGCTCCAAGTGATATGGATACCTCTACTGATGAACAGCAAGCAAGTCTCGAGTCGGATCCCAATTTGGATTCTGAACGAAAAATTGCTGGTGGAGATCTTGAGTCAGAAACAGTGAAGCCAGCTCTTGGCTCGAGTGAGAGTTGTGATGAATATCTCTCTCGGACAGCTCTGATCTTGAAATTTTCAGATTTGGAGTCCGTCCCTTCAGAAAAAAACCTGAAGAAAATATTTAGCCGCTATGGGCCCTTGACTGAAGTGATGAGGAATAACCGCCGGGCCACACTGGTTTTCGAGAAACGTACTGATGCTGAAACAGCTTTCAGCAGCACAGGGAAATACAGTACTTTTGGACCTTCACTTGTCGGTTACAGCCTCAAGTACCTGCCTTCTAAACCAAGTAAAGCTGCTTCTTCAACCAATGCGAAAAAGCGCGGCAGAAAATGTGCAACAGCACCTGAAGGCAATGCAACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

939

Amino Acids

101.89

Weight (kDa)

5.07

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 319 - 401 1.7e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1799
AatII GACGTC 1 cut(s) 21
Acc36I ACCTGC 2 cut(s) 1799, 2730
AccB1I GGYRCC 2 cut(s) 668, 2332
AccB7I CCANNNNNTGG 1 cut(s) 2465
AccII CGCG 2 cut(s) 1672, 2772
AclI AACGTT 1 cut(s) 513
AclWI GGATC 6 cut(s) 564, 1004, 1756, 2069, 2390, 2403
AcsI RAATTY 5 cut(s) 941, 1169, 2126, 2280, 2519
AcuI CTGAAG 8 cut(s) 189, 999, 1581, 1985, 2529, 2579, 2613, 2817
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 5 cut(s) 1669, 2027, 2641, 2683, 2720
AfiI CCNNNNNNNGG 7 cut(s) 41, 68, 281, 679, 891, 2084, 2465
AflIII ACRYGT 1 cut(s) 1653
AhdI GACNNNNNGTC 1 cut(s) 2176
AjnI CCWGG 2 cut(s) 986, 2335
AjuI GAANNNNNNNTTGG 2 cut(s) 2178, 2210
AloI GAACNNNNNNTCC 2 cut(s) 927, 959
Alw21I GWGCWC 2 cut(s) 2249, 2344
Alw26I GTCTC 4 cut(s) 1210, 2055, 2184, 2390
AlwI GGATC 6 cut(s) 564, 1004, 1756, 2069, 2390, 2403
AlwNI CAGNNNCTG 1 cut(s) 2795
Ama87I CYCGRG 6 cut(s) 34, 672, 995, 1675, 2387, 2470
AoxI GGCC 6 cut(s) 574, 992, 1443, 1607, 2582, 2616
ApaI GGGCCC 2 cut(s) 1447, 2586
ApeKI GCWGC 5 cut(s) 1019, 1346, 2066, 2664, 2744
ApoI RAATTY 5 cut(s) 941, 1169, 2126, 2280, 2519
AspLEI GCGC 4 cut(s) 67, 1647, 1674, 2772
AsuC2I CCSGG 6 cut(s) 673, 674, 996, 997, 2123, 2615
AsuHPI GGTGA 7 cut(s) 41, 152, 170, 451, 638, 1340, 1823
AvaI CYCGRG 6 cut(s) 34, 672, 995, 1675, 2387, 2470
AvaII GGWCC 5 cut(s) 4, 272, 2149, 2170, 2690
BaeGI GKGCMC 2 cut(s) 1447, 2586
BamHI GGATCC 1 cut(s) 2395
BanI GGYRCC 2 cut(s) 668, 2332
BanII GRGCYC 5 cut(s) 1447, 1958, 2249, 2344, 2586
BarI GAAGNNNNNNTAC 2 cut(s) 2731, 2763
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 2 cut(s) 2249, 2344
BbvI GCAGC 5 cut(s) 1006, 1333, 2053, 2676, 2731
BccI CCATC 8 cut(s) 146, 185, 422, 745, 758, 884, 1316, 2038
BciT130I CCWGG 2 cut(s) 988, 2337
BciVI GTATCC 3 cut(s) 1914, 2081, 2350
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 6 cut(s) 673, 674, 996, 997, 2123, 2615
BcoDI GTCTC 4 cut(s) 1210, 2055, 2184, 2390
BfaI CTAG 4 cut(s) 770, 938, 959, 1424
BfmI CTRYAG 1 cut(s) 2070
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 2 cut(s) 1799, 2730
BfuI GTATCC 3 cut(s) 1914, 2081, 2350
BglII AGATCT 2 cut(s) 1002, 2434
BisI GCNGC 7 cut(s) 1020, 1347, 2067, 2575, 2665, 2745, 2773
BlpI GCTNAGC 1 cut(s) 1229
BlsI GCNGC 7 cut(s) 1021, 1348, 2068, 2576, 2666, 2746, 2774
BmcAI AGTACT 1 cut(s) 2683
Bme1390I CCNGG 8 cut(s) 673, 674, 988, 996, 997, 2123, 2337, 2615
Bme18I GGWCC 5 cut(s) 4, 272, 2149, 2170, 2690
BmeRI GACNNNNNGTC 1 cut(s) 2176
BmeT110I CYCGRG 6 cut(s) 34, 672, 995, 1675, 2387, 2470
BmiI GGNNCC 8 cut(s) 273, 350, 670, 1445, 1608, 2334, 2397, 2584
BmrFI CCNGG 8 cut(s) 673, 674, 988, 996, 997, 2123, 2337, 2615
BmsI GCATC 7 cut(s) 370, 589, 1031, 1513, 1804, 2034, 2635
BoxI GACNNNNGTC 1 cut(s) 591
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 4 cut(s) 1680, 1716, 2007, 2358
Bpu10I CCTNAGC 1 cut(s) 2289
Bpu1102I GCTNAGC 1 cut(s) 1229
BpuEI CTTGAG 4 cut(s) 1192, 2119, 2459, 2699
BpuMI CCSGG 6 cut(s) 673, 674, 996, 997, 2123, 2615
BsaBI GATNNNNATC 2 cut(s) 954, 2490
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 4 cut(s) 672, 783, 986, 995
BsaXI ACNNNNNCTCC 6 cut(s) 99, 129, 294, 324, 423, 453
Bsc4I CCNNNNNNNGG 7 cut(s) 41, 68, 281, 679, 891, 2084, 2465
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 5 cut(s) 606, 1583, 1733, 1791, 2628
Bse3DI GCAATG 3 cut(s) 628, 1113, 2809
Bse8I GATNNNNATC 2 cut(s) 954, 2490
BseBI CCWGG 2 cut(s) 988, 2337
BseDI CCNNGG 4 cut(s) 672, 783, 986, 995
BseGI GGATG 8 cut(s) 157, 202, 385, 433, 487, 982, 1204, 1600
BseJI GATNNNNATC 2 cut(s) 954, 2490
BseLI CCNNNNNNNGG 7 cut(s) 41, 68, 281, 679, 891, 2084, 2465
BseMI GCAATG 3 cut(s) 628, 1113, 2809
BseNI ACTGG 5 cut(s) 606, 1583, 1733, 1791, 2628
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 2 cut(s) 1447, 2586
BseXI GCAGC 5 cut(s) 1006, 1333, 2053, 2676, 2731
BseYI CCCAGC 2 cut(s) 67, 287
Bsh1236I CGCG 2 cut(s) 1672, 2772
BshFI GGCC 6 cut(s) 576, 994, 1445, 1609, 2584, 2618
BshNI GGYRCC 2 cut(s) 668, 2332
BsiHKAI GWGCWC 2 cut(s) 2249, 2344
BsiHKCI CYCGRG 6 cut(s) 34, 672, 995, 1675, 2387, 2470
BsiSI CCGG 6 cut(s) 62, 673, 996, 2122, 2250, 2614
BslFI GGGAC 1 cut(s) 2525
BslI CCNNNNNNNGG 7 cut(s) 41, 68, 281, 679, 891, 2084, 2465
BsmAI GTCTC 4 cut(s) 1210, 2055, 2184, 2390
BsmFI GGGAC 1 cut(s) 2525
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 6 cut(s) 576, 994, 1445, 1609, 2584, 2618
BsoBI CYCGRG 6 cut(s) 34, 672, 995, 1675, 2387, 2470
Bsp120I GGGCCC 2 cut(s) 1443, 2582
Bsp1286I GDGCHC 5 cut(s) 1447, 1958, 2249, 2344, 2586
Bsp1720I GCTNAGC 1 cut(s) 1229
Bsp19I CCATGG 1 cut(s) 783
BspANI GGCC 6 cut(s) 576, 994, 1445, 1609, 2584, 2618
BspFNI CGCG 2 cut(s) 1672, 2772
BspLI GGNNCC 8 cut(s) 273, 350, 670, 1445, 1608, 2334, 2397, 2584
BspMI ACCTGC 2 cut(s) 1799, 2730
BspPI GGATC 6 cut(s) 564, 1004, 1756, 2069, 2390, 2403
BspT107I GGYRCC 2 cut(s) 668, 2332
BsrDI GCAATG 3 cut(s) 628, 1113, 2809
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 5 cut(s) 606, 1583, 1733, 1791, 2628
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 4 cut(s) 672, 783, 986, 995
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 783
Bst2UI CCWGG 2 cut(s) 988, 2337
Bst4CI ACNGT 4 cut(s) 341, 649, 2452, 2681
Bst6I CTCTTC 3 cut(s) 501, 1312, 1961
BstACI GRCGYC 1 cut(s) 18
BstAPI GCANNNNNTGC 1 cut(s) 1814
BstC8I GCNNGC 6 cut(s) 63, 1765, 1936, 2295, 2379, 2460
BstDSI CCRYGG 1 cut(s) 783
BstF5I GGATG 8 cut(s) 157, 202, 385, 433, 487, 982, 1204, 1600
BstFNI CGCG 2 cut(s) 1672, 2772
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 4 cut(s) 67, 1647, 1674, 2772
BstMAI GTCTC 4 cut(s) 1210, 2055, 2184, 2390
BstMWI GCNNNNNNNGC 5 cut(s) 533, 1117, 1239, 1814, 2654
BstNI CCWGG 2 cut(s) 988, 2337
BstNSI RCATGY 1 cut(s) 1657
BstPAI GACNNNNGTC 1 cut(s) 591
BstSCI CCNGG 8 cut(s) 671, 672, 986, 994, 995, 2121, 2335, 2613
BstSFI CTRYAG 1 cut(s) 2070
BstSLI GKGCMC 2 cut(s) 1447, 2586
BstUI CGCG 2 cut(s) 1672, 2772
BstV1I GCAGC 5 cut(s) 1006, 1333, 2053, 2676, 2731
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 4 cut(s) 1002, 2074, 2395, 2434
BstXI CCANNNNNNTGG 1 cut(s) 1946
BstYI RGATCY 4 cut(s) 1002, 2074, 2395, 2434
BsuI GTATCC 3 cut(s) 1914, 2081, 2350
BsuRI GGCC 6 cut(s) 576, 994, 1445, 1609, 2584, 2618
BtgI CCRYGG 1 cut(s) 783
BtsCI GGATG 8 cut(s) 157, 202, 385, 433, 487, 982, 1204, 1600
BtsI GCAGTG 2 cut(s) 1484, 1559
BtsIMutI CAGTG 7 cut(s) 1032, 1306, 1484, 1559, 1740, 2457, 2621
BveI ACCTGC 2 cut(s) 1799, 2730
Cac8I GCNNGC 6 cut(s) 63, 1765, 1936, 2295, 2379, 2460
CaiI CAGNNNCTG 1 cut(s) 2795
CfoI GCGC 4 cut(s) 67, 1647, 1674, 2772
Cfr10I RCCGGY 1 cut(s) 61
Cfr9I CCCGGG 2 cut(s) 672, 995
Csp6I GTAC 5 cut(s) 1668, 2026, 2640, 2682, 2719
CviAII CATG 9 cut(s) 473, 487, 617, 784, 830, 1162, 1654, 2080, 2107
CviQI GTAC 5 cut(s) 1668, 2026, 2640, 2682, 2719
DraI TTTAAA 2 cut(s) 1048, 1906
DriI GACNNNNNGTC 1 cut(s) 2176
Eam1104I CTCTTC 3 cut(s) 501, 1312, 1961
Eam1105I GACNNNNNGTC 1 cut(s) 2176
EarI CTCTTC 3 cut(s) 501, 1312, 1961
Ecl136II GAGCTC 2 cut(s) 2247, 2342
Eco130I CCWWGG 1 cut(s) 783
Eco24I GRGCYC 5 cut(s) 1447, 1958, 2249, 2344, 2586
Eco47I GGWCC 5 cut(s) 4, 272, 2149, 2170, 2690
Eco53kI GAGCTC 2 cut(s) 2247, 2342
Eco57I CTGAAG 8 cut(s) 189, 999, 1581, 1985, 2529, 2579, 2613, 2817
Eco88I CYCGRG 6 cut(s) 34, 672, 995, 1675, 2387, 2470
EcoICRI GAGCTC 2 cut(s) 2247, 2342
EcoO109I RGGNCCY 2 cut(s) 1443, 2583
EcoRI GAATTC 2 cut(s) 941, 1169
EcoRII CCWGG 2 cut(s) 986, 2335
EcoT14I CCWWGG 1 cut(s) 783
EcoT22I ATGCAT 1 cut(s) 618
EcoT38I GRGCYC 5 cut(s) 1447, 1958, 2249, 2344, 2586
ErhI CCWWGG 1 cut(s) 783
FaeI CATG 9 cut(s) 476, 490, 620, 787, 833, 1165, 1657, 2083, 2110
FalI AAGNNNNNCTT 6 cut(s) 1042, 1074, 1109, 1141, 1333, 1365
FaqI GGGAC 1 cut(s) 2525
FatI CATG 9 cut(s) 472, 486, 616, 783, 829, 1161, 1653, 2079, 2106
FauI CCCGC 2 cut(s) 239, 1206
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 7 cut(s) 1020, 1347, 2067, 2575, 2665, 2745, 2773
FokI GGATG 8 cut(s) 164, 209, 392, 440, 494, 969, 1211, 1607
FriOI GRGCYC 5 cut(s) 1447, 1958, 2249, 2344, 2586
Fsp4HI GCNGC 7 cut(s) 1020, 1347, 2067, 2575, 2665, 2745, 2773
FspBI CTAG 4 cut(s) 770, 938, 959, 1424
GlaI GCGC 4 cut(s) 66, 1646, 1673, 2771
GluI GCNGC 7 cut(s) 1020, 1347, 2067, 2575, 2665, 2745, 2773
GsaI CCCAGC 2 cut(s) 71, 291
GsuI CTGGAG 4 cut(s) 1680, 1716, 2007, 2358
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 6 cut(s) 576, 994, 1445, 1609, 2584, 2618
HapII CCGG 6 cut(s) 62, 673, 996, 2122, 2250, 2614
HhaI GCGC 4 cut(s) 67, 1647, 1674, 2772
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 9 cut(s) 476, 490, 620, 787, 833, 1165, 1657, 2083, 2110
Hin6I GCGC 4 cut(s) 65, 1645, 1672, 2770
HinP1I GCGC 4 cut(s) 65, 1645, 1672, 2770
HincII GTYRAC 2 cut(s) 1417, 1863
HindII GTYRAC 2 cut(s) 1417, 1863
HpaII CCGG 6 cut(s) 62, 673, 996, 2122, 2250, 2614
HphI GGTGA 7 cut(s) 41, 152, 170, 451, 638, 1340, 1823
Hpy166II GTNNAC 4 cut(s) 49, 1417, 1772, 1863
Hpy8I GTNNAC 4 cut(s) 49, 1417, 1772, 1863
Hpy99I CGWCG 2 cut(s) 20, 23
HpyCH4III ACNGT 4 cut(s) 341, 649, 2452, 2681
HpyCH4IV ACGT 4 cut(s) 18, 513, 630, 2638
HpyF10VI GCNNNNNNNGC 5 cut(s) 533, 1117, 1239, 1814, 2654
HpySE526I ACGT 4 cut(s) 18, 513, 630, 2638
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 9 cut(s) 476, 490, 620, 787, 833, 1165, 1657, 2083, 2110
HspAI GCGC 4 cut(s) 65, 1645, 1672, 2770
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 697
LmnI GCTCC 6 cut(s) 213, 541, 568, 2252, 2339, 2347
Lsp1109I GCAGC 5 cut(s) 1006, 1333, 2053, 2676, 2731
LweI GCATC 7 cut(s) 370, 589, 1031, 1513, 1804, 2034, 2635
MaeI CTAG 4 cut(s) 770, 938, 959, 1424
MaeII ACGT 4 cut(s) 18, 513, 630, 2638
MaeIII GTNAC 7 cut(s) 38, 626, 1328, 1432, 1970, 2271, 2705
MfeI CAATTG 1 cut(s) 1182
MflI RGATCY 4 cut(s) 1002, 2074, 2395, 2434
MhlI GDGCHC 5 cut(s) 1447, 1958, 2249, 2344, 2586
MlyI GAGTC 8 cut(s) 601, 716, 988, 1823, 2317, 2399, 2450, 2543
MmeI TCCRAC 4 cut(s) 280, 631, 1891, 2373
Mph1103I ATGCAT 1 cut(s) 618
MroNI GCCGGC 1 cut(s) 61
MslI CAYNNNNRTG 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 1019
MspI CCGG 6 cut(s) 62, 673, 996, 2122, 2250, 2614
MspR9I CCNGG 8 cut(s) 673, 674, 988, 996, 997, 2123, 2337, 2615
MunI CAATTG 1 cut(s) 1182
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 2 cut(s) 988, 2337
MvnI CGCG 2 cut(s) 1672, 2772
MwoI GCNNNNNNNGC 5 cut(s) 533, 1117, 1239, 1814, 2654
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 6 cut(s) 673, 674, 996, 997, 2123, 2615
NcoI CCATGG 1 cut(s) 783
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 9 cut(s) 476, 490, 620, 787, 833, 1165, 1657, 2083, 2110
NlaIV GGNNCC 8 cut(s) 273, 350, 670, 1445, 1608, 2334, 2397, 2584
NmeAIII GCCGAG 1 cut(s) 1811
NmuCI GTSAC 4 cut(s) 38, 626, 1328, 2271
NsiI ATGCAT 1 cut(s) 618
NspI RCATGY 1 cut(s) 1657
PaeR7I CTCGAG 2 cut(s) 2387, 2470
PaqCI CACCTGC 1 cut(s) 1799
PciI ACATGT 1 cut(s) 1653
PcsI WCGNNNNNNNCGW 1 cut(s) 18
PctI GAATGC 1 cut(s) 401
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 164, 950, 1296, 2408
PflMI CCANNNNNTGG 1 cut(s) 2465
PfoI TCCNGGA 1 cut(s) 2121
PkrI GCNGC 7 cut(s) 1021, 1348, 2068, 2576, 2666, 2746, 2774
PleI GAGTC 8 cut(s) 600, 715, 987, 1823, 2316, 2398, 2449, 2542
PpsI GAGTC 8 cut(s) 600, 715, 987, 1823, 2316, 2398, 2449, 2542
PscI ACATGT 1 cut(s) 1653
PshAI GACNNNNGTC 1 cut(s) 591
Psp124BI GAGCTC 2 cut(s) 2249, 2344
Psp1406I AACGTT 1 cut(s) 513
Psp6I CCWGG 2 cut(s) 986, 2335
PspFI CCCAGC 2 cut(s) 67, 287
PspGI CCWGG 2 cut(s) 986, 2335
PspN4I GGNNCC 8 cut(s) 273, 350, 670, 1445, 1608, 2334, 2397, 2584
PspOMI GGGCCC 2 cut(s) 1443, 2582
PspXI VCTCGAGB 1 cut(s) 2470
PstNI CAGNNNCTG 1 cut(s) 2795
PsuI RGATCY 4 cut(s) 1002, 2074, 2395, 2434
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 5 cut(s) 1669, 2027, 2641, 2683, 2720
RsaNI GTAC 5 cut(s) 1668, 2026, 2640, 2682, 2719
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 2 cut(s) 2249, 2344
SatI GCNGC 7 cut(s) 1020, 1347, 2067, 2575, 2665, 2745, 2773
ScaI AGTACT 1 cut(s) 2683
SchI GAGTC 8 cut(s) 601, 716, 988, 1823, 2317, 2399, 2450, 2543
ScrFI CCNGG 8 cut(s) 673, 674, 988, 996, 997, 2123, 2337, 2615
SduI GDGCHC 5 cut(s) 1447, 1958, 2249, 2344, 2586
SfaNI GCATC 7 cut(s) 370, 589, 1031, 1513, 1804, 2034, 2635
SfcI CTRYAG 1 cut(s) 2070
Sfr274I CTCGAG 2 cut(s) 2387, 2470
SinI GGWCC 5 cut(s) 4, 272, 2149, 2170, 2690
SlaI CTCGAG 2 cut(s) 2387, 2470
SmaI CCCGGG 2 cut(s) 674, 997
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 6 cut(s) 1207, 2098, 2387, 2438, 2470, 2714
SmoI CTYRAG 6 cut(s) 1207, 2098, 2387, 2438, 2470, 2714
SrfI GCCCGGGC 1 cut(s) 997
SspI AATATT 1 cut(s) 2568
SspMI CTAG 4 cut(s) 770, 938, 959, 1424
SstI GAGCTC 2 cut(s) 2249, 2344
StyD4I CCNGG 8 cut(s) 671, 672, 986, 994, 995, 2121, 2335, 2613
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 4 cut(s) 341, 649, 2452, 2681
TaiI ACGT 4 cut(s) 21, 516, 633, 2641
TaqI TCGA 5 cut(s) 520, 2311, 2388, 2471, 2631
TaqII GACCGA 1 cut(s) 1535
TatI WGTACW 1 cut(s) 2681
TauI GCSGC 2 cut(s) 2577, 2775
TfiI GAWTC 4 cut(s) 164, 950, 1296, 2408
TscAI CASTG 7 cut(s) 1032, 1306, 1491, 1559, 1740, 2457, 2628
TseFI GTSAC 4 cut(s) 38, 626, 1328, 2271
TseI GCWGC 5 cut(s) 1019, 1346, 2066, 2664, 2744
Tsp45I GTSAC 4 cut(s) 38, 626, 1328, 2271
TspGWI ACGGA 5 cut(s) 935, 1392, 1861, 2100, 2527
TspMI CCCGGG 2 cut(s) 672, 995
TspRI CASTG 7 cut(s) 1032, 1306, 1491, 1559, 1740, 2457, 2628
Van91I CCANNNNNTGG 1 cut(s) 2465
VpaK11BI GGWCC 5 cut(s) 4, 272, 2149, 2170, 2690
XapI RAATTY 5 cut(s) 941, 1169, 2126, 2280, 2519
XceI RCATGY 1 cut(s) 1657
XcmI CCANNNNNNNNNTGG 1 cut(s) 1181
XhoI CTCGAG 2 cut(s) 2387, 2470
XmaI CCCGGG 2 cut(s) 672, 995
XspI CTAG 4 cut(s) 770, 938, 959, 1424
ZraI GACGTC 1 cut(s) 19
ZrmI AGTACT 1 cut(s) 2683
Zsp2I ATGCAT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.