Rw3G013750

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002940
Physical Location & Seq
Forward (+)
13566319 .. 13569689
3371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G013750.1

Sequence Viewer

Length: 3339 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGAGCGTTGATGATAGTGATGCAGTAGTGGGTGAGGACATGAGTGAGGGTTTGGAAGAGGGAGATGAATTGGTAGATGGAGGTGATGATTCTGGAGGGAAAGTAGGACCTGTAGATATGGAGATTGATGAGAAAGGTGGAGATGGAGTGACAGAGGGTTTGGAGGGGACTCAATTTGGGGGTACTGATGTGAATGCAGCGAGTGAGAAAGGGTCGGATTCGGAATCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAATTGTAAGGGAATGCGTGGAACCTGTAGCTGTGGGTACTGTGGAAGGGAACCTCTCGATTAGTTTTGAGGCGAAGGAGGATGGAACAGTTGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCACAAGTAGATGAGGAAGAGAGAGAAAGAGAACAGAATGTCGAGTGTAGCCAGAGTGCCCATGCTACAAGTTCTAAAGTGGAGCATGAGGCTACAGGGTCTGTTGGAGAAATACCGCGGGACATCCCAGCTGTGGAAGTGAAAATTGCCGGTATCGATGAGTCTGGGCATGATGGAGACCTGAATGCAAGCACTGGTTCCCAGATGGTTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGGAGTAAAGACCGGTGAAGATGTCTTGATGCCTGAATATGTTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGATTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCAGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTTTGGATTGGCCTGTTCTTGCATTTCCAAAGAAGTTTATGCAAAGCTCAAAACACAGACGATCATTAATGCTGGAATCAAGGAAGAAGCATGTAGAAGAGATGGTGGTGATAGTTCACTGGGTGCTGCTTCTTTTGAACCCTTGAAACTCATTAACTTTGCAAAACAAGCCGCCAAGCTGCCATTTGGCAGAGCTGACAGACTCGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGGTTGCTGGAAAATGATGCCGACATTTCACTATTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGTTGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCAGAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGATAGTTCCCAACCAGGTAAAAAAGGGAAAAACTCCTCAAATGTAACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTGAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCAAGTAAGAAACGAAAGGCAGTTGATGGAGCTATGCCTGATGACTCTGCGGTGAAACATAGTAAAAGCGCTTCATCAGCAGTGGAAGATATTGTTTCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTACTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAAATCTTCATCGCAGAAACCACGAACTGAAAAGCTGATTGACATGGAGGATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTGCTTGGCTGCCATGAATCCCAATAAAGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCAGTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTGGCAAGCGCAGTAAGAAATCAACCAAGACTGGAAAGAAATCAATCAAACCATGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTCTTATTCTTCTGATAAGATAGTTAAGAGTCAAAATGAAAATGGAGAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTGTGGAGTCACAGTCAAGTCCTGATTCGGAATCACTGAAGCCAGTTCTTGACAATAAAAATGGAGAGTTGGTGTGTGGAACTGCAACAAGCGATAAGGATACTTCTACTGTTGAACTGCAAGCAAGTCTTGTGTTAAATCCCACTTTGGATTCTGAACAGAACATTGTTTGTGGAGATATTGAATCAAAAGCAGTGAACACAGCTGCTACAAGCGATAAGGATACTTATACTGTTGAACTGCAAGCAACTCTCGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGTTTATGGAGATATTGATTCAAAAGCAGTGAACCCAGCTCTGGATGATGAAAATGGAGCATTAGTACGTGAGCCCATCTCTGTTGTTGAGTCACCAGCAGATCCCAAGTTAAATCCCAATTTGGATTTTGAACAGGAAAATACAGTAAGAGATACTGGTGCGGAAGCAGTGAAGCCAGCTCTTGACGATGAAAGTGGAGAGTTGGTGGCTGGAGCTCTAAGTAATAAGGATGCTTCCGCTGTGGATTCGCAAGCAAGTCTTGAGTTCAATCCCAATATGGATTCTGCACTGAAATTTCCTGATGGAGATCATGATTCAGAAGCAGTGAAGCCAGCTTCCCCAAGTGAGAGTTGTGAGGAAGATTCTCCCGCAGCTCTGATCTTGAACTTTTCAGACTTGGAGTCTCTTCCCTCGGAAGCAAGTCTAAACAATATTTTTAGCATCTTCGGGCCTCTGAATGAATCGCAAACCGAATTGTTGAAGAAGACAAACCGGGCCAAAGTGGTTTTCAGGCGGCGTTCTGATGCTCAAAAAGCTTTCAGCAGTGCTGGGAAATACAGTACCTTTGGACCTTCACTTGAAAGTTATCGCCTCAAGTATCCGCTATCACCAAGTAAAGCTTCTCCTTCTCCAAAGGTGGGAAAGCAAGGAAGAATGGATGCATCTGCAGAAGGTGATGGAGCTAGAAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1112

Amino Acids

119.84

Weight (kDa)

4.68

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 296 - 378 2e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1930
Acc36I ACCTGC 1 cut(s) 1930
AccI GTMKAC 1 cut(s) 1664
AccII CGCG 1 cut(s) 562
AciI CCGC 9 cut(s) 560, 562, 1323, 1775, 2804, 2880, 3012, 3157, 3245
AclWI GGATC 6 cut(s) 935, 1693, 2000, 2013, 2157, 2738
AcsI RAATTY 4 cut(s) 872, 1100, 2105, 2936
AcuI CTGAAG 3 cut(s) 930, 1953, 2411
AdeI CACNNNGTG 1 cut(s) 1274
AfaI GTAC 6 cut(s) 235, 346, 1018, 2069, 2709, 3205
AfeI AGCGCT 1 cut(s) 1795
AfiI CCNNNNNNNGG 7 cut(s) 577, 748, 815, 2063, 2064, 2683, 3281
AflIII ACRYGT 1 cut(s) 2348
AgeI ACCGGT 1 cut(s) 707
AhdI GACNNNNNGTC 1 cut(s) 3043
AjnI CCWGG 3 cut(s) 925, 1612, 2124
AjuI GAANNNNNNNTTGG 4 cut(s) 3134, 3166, 3247, 3279
Alw21I GWGCWC 2 cut(s) 1126, 2860
Alw26I GTCTC 4 cut(s) 615, 1141, 2034, 3051
AlwI GGATC 6 cut(s) 935, 1693, 2000, 2013, 2157, 2738
AlwNI CAGNNNCTG 3 cut(s) 545, 1922, 1931
Ama87I CYCGRG 1 cut(s) 2606
Aor51HI AGCGCT 1 cut(s) 1795
AoxI GGCC 5 cut(s) 923, 1161, 1374, 3092, 3138
ApaI GGGCCC 1 cut(s) 1378
ApeKI GCWGC 9 cut(s) 248, 1277, 1330, 1417, 1916, 2045, 2330, 2558, 3014
ApoI RAATTY 4 cut(s) 872, 1100, 2105, 2936
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AseI ATTAAT 1 cut(s) 1218
AsiGI ACCGGT 1 cut(s) 707
AspLEI GCGC 2 cut(s) 1796, 2187
AspS9I GGNCC 7 cut(s) 158, 857, 1374, 1375, 3092, 3138, 3212
AsuC2I CCSGG 1 cut(s) 3137
AsuHPI GGTGA 8 cut(s) 95, 146, 722, 1271, 1789, 2727, 3243, 3329
AvaI CYCGRG 1 cut(s) 2606
AvaII GGWCC 3 cut(s) 158, 857, 3212
BaeGI GKGCMC 2 cut(s) 505, 1378
BaeI ACNNNNGTAYC 4 cut(s) 225, 258, 2337, 2370
BamHI GGATCC 1 cut(s) 2005
BanII GRGCYC 3 cut(s) 1378, 2718, 2860
BarI GAAGNNNNNNTAC 2 cut(s) 1780, 1812
BbsI GAAGAC 1 cut(s) 3134
Bbv12I GWGCWC 2 cut(s) 1126, 2860
BbvI GCAGC 9 cut(s) 260, 1264, 1317, 1404, 1928, 2032, 2317, 2545, 3026
BciT130I CCWGG 3 cut(s) 927, 1614, 2126
BciVI GTATCC 3 cut(s) 2446, 2569, 3252
BcnI CCSGG 1 cut(s) 3137
BcoDI GTCTC 4 cut(s) 615, 1141, 2034, 3051
BfaI CTAG 3 cut(s) 668, 890, 3327
BfmI CTRYAG 6 cut(s) 162, 333, 537, 1864, 1923, 3309
BfoI RGCGCY 1 cut(s) 1797
BfuAI ACCTGC 1 cut(s) 1930
BfuI GTATCC 3 cut(s) 2446, 2569, 3252
Bme1390I CCNGG 4 cut(s) 927, 1614, 2126, 3137
Bme18I GGWCC 3 cut(s) 158, 857, 3212
BmeRI GACNNNNNGTC 1 cut(s) 3043
BmeT110I CYCGRG 1 cut(s) 2606
BmgT120I GGNCC 7 cut(s) 158, 857, 1374, 1375, 3092, 3138, 3212
BmiI GGNNCC 9 cut(s) 330, 359, 643, 807, 859, 911, 1376, 2007, 2130
BmrFI CCNGG 4 cut(s) 927, 1614, 2126, 3137
BmrI ACTGGG 2 cut(s) 1280, 2130
BmuI ACTGGG 2 cut(s) 1280, 2130
BpiI GAAGAC 1 cut(s) 3134
BplI GAGNNNNNCTC 2 cut(s) 2705, 2737
BpmI CTGGAG 3 cut(s) 165, 1617, 2874
BpuEI CTTGAG 4 cut(s) 1123, 1711, 2924, 3221
BpuMI CCSGG 1 cut(s) 3137
Bsa29I ATCGAT 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 2711
BsaBI GATNNNNATC 2 cut(s) 2148, 2949
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 4 cut(s) 560, 917, 926, 3054
BsaWI WCCGGW 1 cut(s) 707
Bsc4I CCNNNNNNNGG 7 cut(s) 577, 748, 815, 2063, 2064, 2683, 3281
Bse118I RCCGGY 2 cut(s) 593, 707
Bse1I ACTGG 7 cut(s) 643, 1275, 1520, 2136, 2212, 2396, 2803
Bse3DI GCAATG 1 cut(s) 1571
Bse8I GATNNNNATC 2 cut(s) 2148, 2949
BseBI CCWGG 3 cut(s) 927, 1614, 2126
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 4 cut(s) 560, 917, 926, 3054
BseJI GATNNNNATC 2 cut(s) 2148, 2949
BseLI CCNNNNNNNGG 7 cut(s) 577, 748, 815, 2063, 2064, 2683, 3281
BseMI GCAATG 1 cut(s) 1571
BseMII CTCAG 4 cut(s) 1427, 1641, 2045, 2238
BseNI ACTGG 7 cut(s) 643, 1275, 1520, 2136, 2212, 2396, 2803
BseRI GAGGAG 3 cut(s) 60, 1558, 1624
BseSI GKGCMC 2 cut(s) 505, 1378
BseXI GCAGC 9 cut(s) 260, 1264, 1317, 1404, 1928, 2032, 2317, 2545, 3026
BseYI CCCAGC 4 cut(s) 571, 742, 2677, 3191
BsgI GTGCAG 1 cut(s) 2913
Bsh1236I CGCG 1 cut(s) 562
BshFI GGCC 5 cut(s) 925, 1163, 1376, 3094, 3140
BshTI ACCGGT 1 cut(s) 707
BshVI ATCGAT 1 cut(s) 600
BsiHKAI GWGCWC 2 cut(s) 1126, 2860
BsiHKCI CYCGRG 1 cut(s) 2606
BsiSI CCGG 3 cut(s) 594, 708, 3136
BslFI GGGAC 4 cut(s) 232, 578, 843, 2178
BslI CCNNNNNNNGG 7 cut(s) 577, 748, 815, 2063, 2064, 2683, 3281
BsmAI GTCTC 4 cut(s) 615, 1141, 2034, 3051
BsmFI GGGAC 4 cut(s) 232, 578, 843, 2178
BsmI GAATGC 3 cut(s) 250, 326, 634
BsnI GGCC 5 cut(s) 925, 1163, 1376, 3094, 3140
Bso31I GGTCTC 1 cut(s) 615
BsoBI CYCGRG 1 cut(s) 2606
Bsp120I GGGCCC 1 cut(s) 1374
Bsp1286I GDGCHC 5 cut(s) 505, 1126, 1378, 2718, 2860
Bsp1407I TGTACA 1 cut(s) 1016
Bsp143I GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
Bsp19I CCATGG 1 cut(s) 917
BspACI CCGC 9 cut(s) 560, 562, 1323, 1775, 2804, 2880, 3012, 3157, 3245
BspANI GGCC 5 cut(s) 925, 1163, 1376, 3094, 3140
BspCNI CTCAG 4 cut(s) 1426, 1642, 2044, 2239
BspDI ATCGAT 1 cut(s) 600
BspFNI CGCG 1 cut(s) 562
BspHI TCATGA 1 cut(s) 2953
BspLI GGNNCC 9 cut(s) 330, 359, 643, 807, 859, 911, 1376, 2007, 2130
BspMAI CTGCAG 2 cut(s) 1927, 3313
BspMI ACCTGC 1 cut(s) 1930
BspPI GGATC 6 cut(s) 935, 1693, 2000, 2013, 2157, 2738
BspTNI GGTCTC 1 cut(s) 615
BsrDI GCAATG 1 cut(s) 1571
BsrFI RCCGGY 2 cut(s) 593, 707
BsrGI TGTACA 1 cut(s) 1016
BsrI ACTGG 7 cut(s) 643, 1275, 1520, 2136, 2212, 2396, 2803
BssAI RCCGGY 2 cut(s) 593, 707
BssECI CCNNGG 4 cut(s) 560, 917, 926, 3054
BssMI GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
BssT1I CCWWGG 1 cut(s) 917
Bst2UI CCWGG 3 cut(s) 927, 1614, 2126
Bst6I CTCTTC 5 cut(s) 102, 429, 456, 1243, 3054
BstAPI GCANNNNNTGC 1 cut(s) 1922
BstAUI TGTACA 1 cut(s) 1016
BstBAI YACGTR 1 cut(s) 2711
BstC8I GCNNGC 8 cut(s) 634, 2183, 2335, 2475, 2598, 2820, 2895, 2976
BstDEI CTNAG 6 cut(s) 291, 1413, 1650, 2031, 2247, 2861
BstDSI CCRYGG 2 cut(s) 560, 917
BstFNI CGCG 1 cut(s) 562
BstH2I RGCGCY 1 cut(s) 1797
BstHHI GCGC 2 cut(s) 1796, 2187
BstKTI GATC 9 cut(s) 889, 943, 1215, 1688, 2008, 2152, 2746, 2953, 3024
BstMAI GTCTC 4 cut(s) 615, 1141, 2034, 3051
BstMBI GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
BstMWI GCNNNNNNNGC 5 cut(s) 1319, 1802, 1922, 2032, 3176
BstNI CCWGG 3 cut(s) 927, 1614, 2126
BstNSI RCATGY 3 cut(s) 1245, 2077, 2352
BstSCI CCNGG 4 cut(s) 925, 1612, 2124, 3135
BstSFI CTRYAG 6 cut(s) 162, 333, 537, 1864, 1923, 3309
BstSLI GKGCMC 2 cut(s) 505, 1378
BstUI CGCG 1 cut(s) 562
BstV1I GCAGC 9 cut(s) 260, 1264, 1317, 1404, 1928, 2032, 2317, 2545, 3026
BstV2I GAAGAC 1 cut(s) 3134
BstX2I RGATCY 2 cut(s) 2005, 2743
BstYI RGATCY 2 cut(s) 2005, 2743
Bsu15I ATCGAT 1 cut(s) 600
BsuI GTATCC 3 cut(s) 2446, 2569, 3252
BsuRI GGCC 5 cut(s) 925, 1163, 1376, 3094, 3140
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 2 cut(s) 560, 917
BtgZI GCGATG 1 cut(s) 1950
BtsI GCAGTG 6 cut(s) 1812, 2553, 2676, 2817, 2973, 3193
BveI ACCTGC 1 cut(s) 1930
Cac8I GCNNGC 8 cut(s) 634, 2183, 2335, 2475, 2598, 2820, 2895, 2976
CaiI CAGNNNCTG 3 cut(s) 545, 1922, 1931
CciI TCATGA 1 cut(s) 2953
CfoI GCGC 2 cut(s) 1796, 2187
Cfr10I RCCGGY 2 cut(s) 593, 707
Cfr13I GGNCC 7 cut(s) 158, 857, 1374, 1375, 3092, 3138, 3212
Cfr42I CCGCGG 1 cut(s) 563
ClaI ATCGAT 1 cut(s) 600
CsiI ACCWGGT 1 cut(s) 1612
Csp6I GTAC 6 cut(s) 234, 345, 1017, 2068, 2708, 3204
CspAI ACCGGT 1 cut(s) 707
CviQI GTAC 6 cut(s) 234, 345, 1017, 2068, 2708, 3204
DdeI CTNAG 6 cut(s) 291, 1413, 1650, 2031, 2247, 2861
DpnI GATC 9 cut(s) 888, 942, 1214, 1687, 2007, 2151, 2745, 2952, 3023
DpnII GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
DraIII CACNNNGTG 1 cut(s) 1274
DriI GACNNNNNGTC 1 cut(s) 3043
Eam1104I CTCTTC 5 cut(s) 102, 429, 456, 1243, 3054
Eam1105I GACNNNNNGTC 1 cut(s) 3043
EarI CTCTTC 5 cut(s) 102, 429, 456, 1243, 3054
Ecl136II GAGCTC 1 cut(s) 2858
Eco130I CCWWGG 1 cut(s) 917
Eco24I GRGCYC 3 cut(s) 1378, 2718, 2860
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 3 cut(s) 158, 857, 3212
Eco47III AGCGCT 1 cut(s) 1795
Eco53kI GAGCTC 1 cut(s) 2858
Eco57I CTGAAG 3 cut(s) 930, 1953, 2411
Eco88I CYCGRG 1 cut(s) 2606
EcoICRI GAGCTC 1 cut(s) 2858
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1100
EcoRII CCWGG 3 cut(s) 925, 1612, 2124
EcoT14I CCWWGG 1 cut(s) 917
EcoT22I ATGCAT 2 cut(s) 2234, 3307
EcoT38I GRGCYC 3 cut(s) 1378, 2718, 2860
ErhI CCWWGG 1 cut(s) 917
FalI AAGNNNNNCTT 8 cut(s) 1500, 1532, 2466, 2498, 2886, 2918, 3247, 3279
FaqI GGGAC 4 cut(s) 232, 578, 843, 2178
FauI CCCGC 2 cut(s) 555, 3019
FblI GTMKAC 1 cut(s) 1664
FriOI GRGCYC 3 cut(s) 1378, 2718, 2860
FspBI CTAG 3 cut(s) 668, 890, 3327
GlaI GCGC 2 cut(s) 1795, 2186
GsaI CCCAGC 4 cut(s) 575, 746, 2681, 3195
GsuI CTGGAG 3 cut(s) 165, 1617, 2874
HaeII RGCGCY 1 cut(s) 1797
HaeIII GGCC 5 cut(s) 925, 1163, 1376, 3094, 3140
HapII CCGG 3 cut(s) 594, 708, 3136
HhaI GCGC 2 cut(s) 1796, 2187
Hin6I GCGC 2 cut(s) 1794, 2185
HinP1I GCGC 2 cut(s) 1794, 2185
HindIII AAGCTT 3 cut(s) 879, 3177, 3261
HpaII CCGG 3 cut(s) 594, 708, 3136
HphI GGTGA 8 cut(s) 95, 146, 722, 1271, 1789, 2727, 3243, 3329
Hpy166II GTNNAC 5 cut(s) 665, 1268, 1665, 2551, 2674
Hpy8I GTNNAC 5 cut(s) 665, 1268, 1665, 2551, 2674
HpyCH4IV ACGT 2 cut(s) 680, 2710
HpyF10VI GCNNNNNNNGC 5 cut(s) 1319, 1802, 1922, 2032, 3176
HpyF3I CTNAG 6 cut(s) 291, 1413, 1650, 2031, 2247, 2861
HpySE526I ACGT 2 cut(s) 680, 2710
HspAI GCGC 2 cut(s) 1794, 2185
KspI CCGCGG 1 cut(s) 563
Kzo9I GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
Lsp1109I GCAGC 9 cut(s) 260, 1264, 1317, 1404, 1928, 2032, 2317, 2545, 3026
MabI ACCWGGT 1 cut(s) 1612
MaeI CTAG 3 cut(s) 668, 890, 3327
MaeII ACGT 2 cut(s) 680, 2710
MaeIII GTNAC 5 cut(s) 199, 1363, 1642, 2361, 2733
MalI GATC 9 cut(s) 888, 942, 1214, 1687, 2007, 2151, 2745, 2952, 3023
MboI GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
MfeI CAATTG 2 cut(s) 309, 2153
MflI RGATCY 2 cut(s) 2005, 2743
MhlI GDGCHC 5 cut(s) 505, 1126, 1378, 2718, 2860
MlyI GAGTC 9 cut(s) 214, 614, 1347, 1763, 2261, 2305, 2369, 2741, 3053
MmeI TCCRAC 6 cut(s) 246, 529, 663, 1379, 1831, 2232
Mph1103I ATGCAT 2 cut(s) 2234, 3307
MspA1I CMGCKG 5 cut(s) 562, 575, 1417, 2558, 2882
MspI CCGG 3 cut(s) 594, 708, 3136
MspR9I CCNGG 4 cut(s) 927, 1614, 2126, 3137
MunI CAATTG 2 cut(s) 309, 2153
Mva1269I GAATGC 3 cut(s) 250, 326, 634
MvaI CCWGG 3 cut(s) 927, 1614, 2126
MvnI CGCG 1 cut(s) 562
MwoI GCNNNNNNNGC 5 cut(s) 1319, 1802, 1922, 2032, 3176
NciI CCSGG 1 cut(s) 3137
NcoI CCATGG 1 cut(s) 917
NdeII GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
NlaIV GGNNCC 9 cut(s) 330, 359, 643, 807, 859, 911, 1376, 2007, 2130
NmuCI GTSAC 3 cut(s) 199, 2361, 2733
NsiI ATGCAT 2 cut(s) 2234, 3307
NspI RCATGY 3 cut(s) 1245, 2077, 2352
PaeR7I CTCGAG 1 cut(s) 2606
PagI TCATGA 1 cut(s) 2953
PaqCI CACCTGC 1 cut(s) 1930
PciI ACATGT 1 cut(s) 2348
PctI GAATGC 3 cut(s) 250, 326, 634
PinAI ACCGGT 1 cut(s) 707
PleI GAGTC 9 cut(s) 214, 613, 1347, 1763, 2261, 2304, 2368, 2740, 3052
PpsI GAGTC 9 cut(s) 214, 613, 1347, 1763, 2261, 2304, 2368, 2740, 3052
Ppu21I YACGTR 1 cut(s) 2711
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2348
PshBI ATTAAT 1 cut(s) 1218
Psp124BI GAGCTC 1 cut(s) 2860
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 925, 1612, 2124
PspFI CCCAGC 4 cut(s) 571, 742, 2677, 3191
PspGI CCWGG 3 cut(s) 925, 1612, 2124
PspN4I GGNNCC 9 cut(s) 330, 359, 643, 807, 859, 911, 1376, 2007, 2130
PspOMI GGGCCC 1 cut(s) 1374
PspPI GGNCC 7 cut(s) 158, 857, 1374, 1375, 3092, 3138, 3212
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 1927, 3313
PstNI CAGNNNCTG 3 cut(s) 545, 1922, 1931
PsuI RGATCY 2 cut(s) 2005, 2743
PvuII CAGCTG 3 cut(s) 575, 1417, 2558
RsaI GTAC 6 cut(s) 235, 346, 1018, 2069, 2709, 3205
RsaNI GTAC 6 cut(s) 234, 345, 1017, 2068, 2708, 3204
SacI GAGCTC 1 cut(s) 2860
SacII CCGCGG 1 cut(s) 563
Sau3AI GATC 9 cut(s) 886, 940, 1212, 1685, 2005, 2149, 2743, 2950, 3021
Sau96I GGNCC 7 cut(s) 158, 857, 1374, 1375, 3092, 3138, 3212
SchI GAGTC 9 cut(s) 214, 614, 1347, 1763, 2261, 2305, 2369, 2741, 3053
ScrFI CCNGG 4 cut(s) 927, 1614, 2126, 3137
SduI GDGCHC 5 cut(s) 505, 1126, 1378, 2718, 2860
SexAI ACCWGGT 1 cut(s) 1612
SfcI CTRYAG 6 cut(s) 162, 333, 537, 1864, 1923, 3309
Sfr274I CTCGAG 1 cut(s) 2606
Sfr303I CCGCGG 1 cut(s) 563
SgrBI CCGCGG 1 cut(s) 563
SinI GGWCC 3 cut(s) 158, 857, 3212
SlaI CTCGAG 1 cut(s) 2606
SmlI CTYRAG 5 cut(s) 1138, 1726, 2606, 2903, 3236
SmoI CTYRAG 5 cut(s) 1138, 1726, 2606, 2903, 3236
SsiI CCGC 9 cut(s) 560, 562, 1323, 1775, 2804, 2880, 3012, 3157, 3245
SspI AATATT 3 cut(s) 1902, 2172, 3076
SspMI CTAG 3 cut(s) 668, 890, 3327
SstI GAGCTC 1 cut(s) 2860
StyD4I CCNGG 4 cut(s) 925, 1612, 2124, 3135
StyI CCWWGG 1 cut(s) 917
TaiI ACGT 2 cut(s) 683, 2713
TaqI TCGA 6 cut(s) 278, 365, 486, 600, 1356, 2607
TatI WGTACW 1 cut(s) 1016
TauI GCSGC 2 cut(s) 1325, 3160
TseFI GTSAC 3 cut(s) 199, 2361, 2733
TseI GCWGC 9 cut(s) 248, 1277, 1330, 1417, 1916, 2045, 2330, 2558, 3014
Tsp45I GTSAC 3 cut(s) 199, 2361, 2733
VpaK11BI GGWCC 3 cut(s) 158, 857, 3212
VspI ATTAAT 1 cut(s) 1218
XapI RAATTY 4 cut(s) 872, 1100, 2105, 2936
XceI RCATGY 3 cut(s) 1245, 2077, 2352
XhoI CTCGAG 1 cut(s) 2606
XmiI GTMKAC 1 cut(s) 1664
XspI CTAG 3 cut(s) 668, 890, 3327
Zsp2I ATGCAT 2 cut(s) 2234, 3307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.