RLG00000024412

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002940
Physical Location & Seq
Reverse (-)
35591040 .. 35593432
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024412

Sequence Viewer

Length: 1704 bp
ATGGATGGGAACATGTTTAGTGATGTGAATGGACAGTATGATCCCAAACCGGAATTCCATGTTTATGATATAGTATGGGGGAAAGTAAGGGGTTATCCCTGGTGGCCTGGACGGATATGTGATCCTTCAGCTTCATCGAATGAGGCAAAGATGCTTTTCAGAAAGGGTAGTTATTTGATAGATTATTTTGGGGATCACTCATTTACTTGGAATGAACCACCACATATTAAGCCATTTCTGGATTATTTCTCGCAAATGGAGAAGCAGAGCAACAAGGACGAATTCCAAAATGCCATTGCTTGTGCTCTGGACGAATTCTTAAGACGGGTAGAGTTGGGGGTGGTCTGTTCTTGCATATCTGGAGAGGTATATCCAAAACTCAAAAATCAGATAATTATTAGTGATGGAGTCCGGCAAGAAGAAGAAGAAGAAGACAGTGGAAATGCTGGTGACGGTTCCCTTACCGCCATCTCTTTTGAACCTCAGAAACTCATTATGCTTCTAAAGGAGTTTGCTCAGTTTTCTTATGGAAAAGTTGACCAACTAGAACTTTCAACATCAGTGATCCAATTATCAGCTTTCTATTGTTGGAAGAGTTCTCAGCTGTCGCTGCCAACGGAGAATGATGCCTATATATCAGTATTGGGGGAGAAGACTCAATGCAATGTATTAGGTCACAATTCACTTCCAGTAACTACAGATGAGAAGCTGGTGCAGAAATCTAAAAGTAAAGCTAACAACAATGATCTGGGATGTTGTGCAACATTATGGCTGATGAAGAAGCAACGCACAATTCACAACCGCAGGAATAAAATCCTGGTTCCGCCCCTGTGTTTGTCTATACCAATGACTGATAATGCAGCAGAAGGGGAAGCTAGTGGTTTGGTTGTTTTACCTTCCATGAGTGAGAATACATCAGAAGGGGAAACTAGCAGTAAATTGATTTTGCAATCTTCTGATGAGAATCCAGAGGAAGTTGATGCTATAGCGGATGAAGATGTGACGGCAGTAAGTGAAGCTGCACTTCCAGTAACAAAGGATGTGAAATTGTTGTGGAAATCAGAAGGTAAAGATAACTCATTGCACAAGTGTAATCTCATGTCTGAGTGCAGTATGAATTCGAGTGAAAAGGAGAAAATATTGTCAGATTTAAAGGCTTATAAGTGTTTGTCTACTCCAATATGTCAGAACGGATCAGAAGGGAAAGCAGGCAGTAAATTGACTTTGCAATCCTGTGTAAAGCGCAAGGCAGTTGATGTTATGGCTGATGACTTTGCGCTGAAACATAGGAAAAGTGATTCATCAAAGAGGACTAAAGATGCACTTCTGCTAACTAAGGATGAGATGTTAGTGAGGAAAACCGAAAGTAAAAGTAACTCGATGCATAAGTGCAAGCACCTATATGGAGATAAAATGAATCCAAGTAAAGAACAGAAAAGCTTATCAGATTTAAATGGTGAGAAGTGTTTGTCTCCAACAAGTGAGTATAGATTACCTTCTGATCATACATTGACTTTGCACTCCTCTAGCAGTATACAAAAGGAAGTTGATCCTATAGCTGATGACTCTGCACTGGAAGATTGGGAAAGCTATTTATCAGCAGGGGGTGTTGAGCAGTCTTTTGGAGTCGCAGATTTTATTAACATGTTTCTACAGAGGGAGCACACTTCTGGAGCTGGAGAAAGGATCTGTCTGGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

568

Amino Acids

63.13

Weight (kDa)

5.2

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 23 - 105 3.5e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccI GTMKAC 2 cut(s) 1172, 1534
AciI CCGC 4 cut(s) 465, 802, 824, 989
AclWI GGATC 7 cut(s) 35, 116, 201, 559, 1201, 1544, 1694
AcsI RAATTY 4 cut(s) 53, 281, 314, 1117
AcuI CTGAAG 1 cut(s) 111
AflII CTTAAG 1 cut(s) 319
AflIII ACRYGT 2 cut(s) 12, 1644
AgsI TTSAA 3 cut(s) 479, 555, 1700
AjnI CCWGG 3 cut(s) 98, 106, 816
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
Alw21I GWGCWC 2 cut(s) 307, 1665
Alw26I GTCTC 1 cut(s) 1476
AlwI GGATC 7 cut(s) 35, 116, 201, 559, 1201, 1544, 1694
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 3 cut(s) 610, 860, 1019
ApoI RAATTY 4 cut(s) 53, 281, 314, 1117
AspLEI GCGC 2 cut(s) 1245, 1279
AsuHPI GGTGA 2 cut(s) 461, 1469
BbsI GAAGAC 2 cut(s) 438, 659
Bbv12I GWGCWC 2 cut(s) 307, 1665
BbvI GCAGC 3 cut(s) 597, 872, 1006
BccI CCATC 2 cut(s) 398, 476
BceAI ACGGC 1 cut(s) 1020
BciT130I CCWGG 3 cut(s) 100, 108, 818
BclI TGATCA 1 cut(s) 1501
BcoDI GTCTC 1 cut(s) 1476
BfaI CTAG 4 cut(s) 545, 876, 930, 1527
BfmI CTRYAG 4 cut(s) 696, 984, 1554, 1652
BfrI CTTAAG 1 cut(s) 319
BisI GCNGC 3 cut(s) 611, 861, 1020
BlsI GCNGC 3 cut(s) 612, 862, 1021
Bme1390I CCNGG 3 cut(s) 100, 108, 818
BmiI GGNNCC 2 cut(s) 457, 822
BmrFI CCNGG 3 cut(s) 100, 108, 818
BmsI GCATC 5 cut(s) 141, 616, 970, 1309, 1371
BpiI GAAGAC 2 cut(s) 438, 659
BpmI CTGGAG 3 cut(s) 381, 1692, 1698
BsaBI GATNNNNATC 1 cut(s) 963
BsaJI CCNNGG 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 49
Bse1I ACTGG 3 cut(s) 689, 1028, 1578
Bse3DI GCAATG 3 cut(s) 294, 670, 1079
Bse8I GATNNNNATC 1 cut(s) 963
BseBI CCWGG 3 cut(s) 100, 108, 818
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 5 cut(s) 10, 758, 997, 1045, 1345
BseJI GATNNNNATC 1 cut(s) 963
BseMI GCAATG 3 cut(s) 294, 670, 1079
BseMII CTCAG 4 cut(s) 497, 530, 614, 1095
BseNI ACTGG 3 cut(s) 689, 1028, 1578
BseRI GAGGAG 1 cut(s) 1513
BseXI GCAGC 3 cut(s) 597, 872, 1006
BsgI GTGCAG 4 cut(s) 734, 1005, 1129, 1554
BshFI GGCC 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 307, 1665
BsiSI CCGG 2 cut(s) 50, 412
BsmAI GTCTC 1 cut(s) 1476
BsnI GGCC 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 307, 1665
Bsp143I GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
BspACI CCGC 4 cut(s) 465, 802, 824, 989
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 4 cut(s) 496, 529, 613, 1096
BspLI GGNNCC 2 cut(s) 457, 822
BspPI GGATC 7 cut(s) 35, 116, 201, 559, 1201, 1544, 1694
BspTI CTTAAG 1 cut(s) 319
BsrDI GCAATG 3 cut(s) 294, 670, 1079
BsrI ACTGG 3 cut(s) 689, 1028, 1578
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
BssNAI GTATAC 1 cut(s) 1535
Bst1107I GTATAC 1 cut(s) 1535
Bst2UI CCWGG 3 cut(s) 100, 108, 818
Bst4CI ACNGT 3 cut(s) 36, 437, 455
Bst6I CTCTTC 1 cut(s) 587
BstAFI CTTAAG 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 1210, 1394
BstDEI CTNAG 5 cut(s) 483, 516, 600, 1104, 1335
BstF5I GGATG 5 cut(s) 10, 758, 997, 1045, 1345
BstHHI GCGC 2 cut(s) 1245, 1279
BstKTI GATC 9 cut(s) 43, 124, 196, 567, 748, 1196, 1504, 1552, 1689
BstMAI GTCTC 1 cut(s) 1476
BstMBI GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
BstMWI GCNNNNNNNGC 1 cut(s) 610
BstNI CCWGG 3 cut(s) 100, 108, 818
BstNSI RCATGY 2 cut(s) 16, 1648
BstSCI CCNGG 3 cut(s) 98, 106, 816
BstSFI CTRYAG 4 cut(s) 696, 984, 1554, 1652
BstV1I GCAGC 3 cut(s) 597, 872, 1006
BstV2I GAAGAC 2 cut(s) 438, 659
BstX2I RGATCY 1 cut(s) 1686
BstYI RGATCY 1 cut(s) 1686
BstZ17I GTATAC 1 cut(s) 1535
BsuRI GGCC 1 cut(s) 106
BtsCI GGATG 5 cut(s) 10, 758, 997, 1045, 1345
BtsIMutI CAGTG 3 cut(s) 442, 567, 1571
Cac8I GCNNGC 2 cut(s) 1210, 1394
CfoI GCGC 2 cut(s) 1245, 1279
CviAII CATG 5 cut(s) 13, 59, 901, 1099, 1645
DdeI CTNAG 5 cut(s) 483, 516, 600, 1104, 1335
DpnI GATC 9 cut(s) 42, 123, 195, 566, 747, 1195, 1503, 1551, 1688
DpnII GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
DraI TTTAAA 2 cut(s) 1152, 1452
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
EciI GGCGGA 1 cut(s) 813
Eco57I CTGAAG 1 cut(s) 111
EcoRI GAATTC 4 cut(s) 53, 281, 314, 1117
EcoRII CCWGG 3 cut(s) 98, 106, 816
EcoT22I ATGCAT 1 cut(s) 1386
FaeI CATG 5 cut(s) 16, 62, 904, 1102, 1648
FalI AAGNNNNNCTT 4 cut(s) 1008, 1040, 1308, 1340
FatI CATG 5 cut(s) 12, 58, 900, 1098, 1644
FbaI TGATCA 1 cut(s) 1501
FblI GTMKAC 2 cut(s) 1172, 1534
Fnu4HI GCNGC 3 cut(s) 611, 861, 1020
FokI GGATG 5 cut(s) 17, 765, 1004, 1052, 1352
Fsp4HI GCNGC 3 cut(s) 611, 861, 1020
FspBI CTAG 4 cut(s) 545, 876, 930, 1527
GlaI GCGC 2 cut(s) 1244, 1278
GluI GCNGC 3 cut(s) 611, 861, 1020
GsuI CTGGAG 3 cut(s) 381, 1692, 1698
HaeIII GGCC 1 cut(s) 106
HapII CCGG 2 cut(s) 50, 412
HhaI GCGC 2 cut(s) 1245, 1279
Hin1II CATG 5 cut(s) 16, 62, 904, 1102, 1648
Hin6I GCGC 2 cut(s) 1243, 1277
HinP1I GCGC 2 cut(s) 1243, 1277
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 1438
HinfI GANTC 7 cut(s) 408, 655, 964, 1298, 1417, 1565, 1626
HpaII CCGG 2 cut(s) 50, 412
HphI GGTGA 2 cut(s) 461, 1469
Hpy166II GTNNAC 3 cut(s) 538, 1173, 1535
Hpy188III TCNNGA 5 cut(s) 239, 308, 360, 968, 1671
Hpy8I GTNNAC 3 cut(s) 538, 1173, 1535
HpyAV CCTTC 7 cut(s) 135, 860, 906, 914, 1058, 1193, 1506
HpyCH4III ACNGT 3 cut(s) 36, 437, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 610
HpyF3I CTNAG 5 cut(s) 483, 516, 600, 1104, 1335
Hsp92II CATG 5 cut(s) 16, 62, 904, 1102, 1648
HspAI GCGC 2 cut(s) 1243, 1277
Ksp22I TGATCA 1 cut(s) 1501
Kzo9I GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
LmnI GCTCC 2 cut(s) 1660, 1673
Lsp1109I GCAGC 3 cut(s) 597, 872, 1006
LweI GCATC 5 cut(s) 141, 616, 970, 1309, 1371
MaeI CTAG 4 cut(s) 545, 876, 930, 1527
MaeIII GTNAC 6 cut(s) 449, 674, 691, 1000, 1030, 1373
MalI GATC 9 cut(s) 42, 123, 195, 566, 747, 1195, 1503, 1551, 1688
MboI GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
MflI RGATCY 1 cut(s) 1686
MhlI GDGCHC 2 cut(s) 307, 1665
MlyI GAGTC 4 cut(s) 417, 649, 1559, 1635
MmeI TCCRAC 2 cut(s) 569, 1499
MnlI CCTC 8 cut(s) 136, 358, 492, 964, 1302, 1347, 1534, 1650
Mph1103I ATGCAT 1 cut(s) 1386
MseI TTAA 5 cut(s) 228, 320, 1151, 1451, 1641
MslI CAYNNNNRTG 2 cut(s) 63, 1401
MspA1I CMGCKG 1 cut(s) 604
MspCI CTTAAG 1 cut(s) 319
MspI CCGG 2 cut(s) 50, 412
MspR9I CCNGG 3 cut(s) 100, 108, 818
MvaI CCWGG 3 cut(s) 100, 108, 818
MwoI GCNNNNNNNGC 1 cut(s) 610
NdeII GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
NlaIII CATG 5 cut(s) 16, 62, 904, 1102, 1648
NlaIV GGNNCC 2 cut(s) 457, 822
NmuCI GTSAC 3 cut(s) 449, 674, 1000
NsiI ATGCAT 1 cut(s) 1386
NspI RCATGY 2 cut(s) 16, 1648
PciI ACATGT 2 cut(s) 12, 1644
PfeI GAWTC 3 cut(s) 964, 1298, 1417
PkrI GCNGC 3 cut(s) 612, 862, 1021
PleI GAGTC 4 cut(s) 416, 649, 1559, 1634
PpsI GAGTC 4 cut(s) 416, 649, 1559, 1634
PscI ACATGT 2 cut(s) 12, 1644
PsiI TTATAA 1 cut(s) 1161
Psp6I CCWGG 3 cut(s) 98, 106, 816
PspGI CCWGG 3 cut(s) 98, 106, 816
PspN4I GGNNCC 2 cut(s) 457, 822
PsuI RGATCY 1 cut(s) 1686
PvuII CAGCTG 1 cut(s) 604
RseI CAYNNNNRTG 2 cut(s) 63, 1401
SaqAI TTAA 5 cut(s) 228, 320, 1151, 1451, 1641
SatI GCNGC 3 cut(s) 611, 861, 1020
Sau3AI GATC 9 cut(s) 40, 121, 193, 564, 745, 1193, 1501, 1549, 1686
SchI GAGTC 4 cut(s) 417, 649, 1559, 1635
ScrFI CCNGG 3 cut(s) 100, 108, 818
SduI GDGCHC 2 cut(s) 307, 1665
SfaNI GCATC 5 cut(s) 141, 616, 970, 1309, 1371
SfcI CTRYAG 4 cut(s) 696, 984, 1554, 1652
SmiI ATTTAAAT 1 cut(s) 1452
SmiMI CAYNNNNRTG 2 cut(s) 63, 1401
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
SsiI CCGC 4 cut(s) 465, 802, 824, 989
SspI AATATT 1 cut(s) 1140
SspMI CTAG 4 cut(s) 545, 876, 930, 1527
StyD4I CCNGG 3 cut(s) 98, 106, 816
SwaI ATTTAAAT 1 cut(s) 1452
TaaI ACNGT 3 cut(s) 36, 437, 455
TaqI TCGA 3 cut(s) 137, 1121, 1379
TfiI GAWTC 3 cut(s) 964, 1298, 1417
Tru1I TTAA 5 cut(s) 228, 320, 1151, 1451, 1641
Tru9I TTAA 5 cut(s) 228, 320, 1151, 1451, 1641
TscAI CASTG 3 cut(s) 442, 567, 1578
TseFI GTSAC 3 cut(s) 449, 674, 1000
TseI GCWGC 3 cut(s) 610, 860, 1019
Tsp45I GTSAC 3 cut(s) 449, 674, 1000
TspDTI ATGAA 7 cut(s) 123, 228, 791, 1008, 1130, 1290, 1430
TspGWI ACGGA 3 cut(s) 127, 632, 1206
TspRI CASTG 3 cut(s) 442, 567, 1578
Vha464I CTTAAG 1 cut(s) 319
XapI RAATTY 4 cut(s) 53, 281, 314, 1117
XceI RCATGY 2 cut(s) 16, 1648
XmiI GTMKAC 2 cut(s) 1172, 1534
XspI CTAG 4 cut(s) 545, 876, 930, 1527
Zsp2I ATGCAT 1 cut(s) 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.