Rh3DG166800

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002940
Physical Location & Seq
Forward (+)
14519670 .. 14521463
1794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG166800.1

Sequence Viewer

Length: 1227 bp
ATGCCCAGTGGAAGCACTCATTCTCGTACTGAAAGTGTGCATACCTTGCTTAATATTGTTGATTTGGAGACGTATACGAGAATGGATGGGAACATGTTTAGTGATGTGAATGGACAGTATGATCCAAAACCGGAATTCCATGTTTATGATATAGTATGGGGGAAAGTAAGGGGTTATCCCTGGTGGCCTGGACGGATATGTGATCCTTCAGCTTCATCGAATGAGGCAAAGATGCTTTTCAGAAAGGGTAGTTATTTGATAGATTATTTTGGGGATCACTCATTTACTTGGAATGAAGCACCACATATTAAGCCATTTCTGGATTATTTCTCGCAAATGGAGAAGCAGAGCAACAAGGACGAATTCCAAAATGCCATTGCTTGTGCTCTGGACGAATTCTTAAGACAGGTAGAGTTGGGGTTGGTCTGTTCTTGCATATCTGGAGAGGTATATCCAAAACTCAAAAATCAGATAATTATTAGTGATGGAGTCAGGCAAGAAGAAGAAGAAGAAGAAGAAGACAGTGGAAATGCTGGTGACGGTTCCCTTACCGCCGTCTCTTTTGAACCTCAGAAACTCATTATGCTTCTAAAGGAGTTTGCTCAGTTTTCATATGGAAAAGCTGACCAACTAGAACTTTCAACATCAGTGATCCAATTATCAGCTTTCTATTGTTGGAAGAGTTCTCAGCTGTCACTGCCAACGGAGAATGATGCCTATATATCAGTATTGGGGGAGAAGACTCAATGCAATGTATTAGGTCACAATTCACTTCCAGTAACTACAGATGAGAAGCTGGTGCAGAAATCTAAAAGTAAAGCTAACAACAATGATCTGGGATGTTGTGCAACATTATGGCTGATGAAGAAGCAACGCACAATTCACACCCGCAGGAATAAAATCCTGGTTCCGCCCCTGTGTTTGTCTATTCCAATGACTGATAATGCAGCAGAAGGGGAAGCTAGTGGTTTGGTTGTTTTACCTTCCATGAGTGAGAATACATCAGAAGGGGAAACTAGCAGTGAATTGATTTTGCAATCTTCTGATGAGAATCCAGAGGAAGTTGATGCTATAGCGGATGAAGAATATTGTCATATGGATTTTGTGACCAATGAACCTGTTTTTAGTTTTACTAATAGCTTTGTTTTTTGGGTAAATTCGGTGGCCATTATCTTAGTCATTACAGAATGTAAGAATATTGTCAGTACACAAAAGAGACAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

408

Amino Acids

45.9

Weight (kDa)

4.73

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 50 - 132 4.3e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 74
AciI CCGC 4 cut(s) 552, 889, 911, 1076
AclWI GGATC 4 cut(s) 116, 197, 282, 646
AcoI YGGCCR 1 cut(s) 1164
AcsI RAATTY 4 cut(s) 134, 362, 395, 1156
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 2 cut(s) 28, 1207
AflII CTTAAG 1 cut(s) 400
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 2 cut(s) 566, 642
AjnI CCWGG 3 cut(s) 179, 187, 903
AluBI AGCT 8 cut(s) 212, 623, 665, 691, 796, 821, 962, 1140
AluI AGCT 8 cut(s) 212, 623, 665, 691, 796, 821, 962, 1140
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 3 cut(s) 62, 562, 1210
AlwI GGATC 4 cut(s) 116, 197, 282, 646
AoxI GGCC 2 cut(s) 185, 1164
ApeKI GCWGC 1 cut(s) 947
ApoI RAATTY 4 cut(s) 134, 362, 395, 1156
AsuHPI GGTGA 1 cut(s) 548
BalI TGGCCA 1 cut(s) 1166
BbsI GAAGAC 2 cut(s) 525, 746
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 959
BccI CCATC 2 cut(s) 80, 479
BceAI ACGGC 1 cut(s) 539
BciT130I CCWGG 3 cut(s) 181, 189, 905
BcoDI GTCTC 3 cut(s) 62, 562, 1210
BfaI CTAG 3 cut(s) 632, 963, 1017
BfmI CTRYAG 2 cut(s) 783, 1071
BfrI CTTAAG 1 cut(s) 400
BisI GCNGC 1 cut(s) 948
BlsI GCNGC 1 cut(s) 949
Bme1390I CCNGG 3 cut(s) 181, 189, 905
BmiI GGNNCC 2 cut(s) 544, 909
BmrFI CCNGG 3 cut(s) 181, 189, 905
BmsI GCATC 3 cut(s) 222, 703, 1057
BpiI GAAGAC 2 cut(s) 525, 746
BpmI CTGGAG 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 1050
BsaJI CCNNGG 1 cut(s) 179
BsaWI WCCGGW 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 6, 776
Bse3DI GCAATG 2 cut(s) 375, 757
Bse8I GATNNNNATC 1 cut(s) 1050
BseBI CCWGG 3 cut(s) 181, 189, 905
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 3 cut(s) 91, 845, 1084
BseJI GATNNNNATC 1 cut(s) 1050
BseMI GCAATG 2 cut(s) 375, 757
BseMII CTCAG 3 cut(s) 584, 617, 701
BseNI ACTGG 2 cut(s) 6, 776
BseXI GCAGC 1 cut(s) 959
BsgI GTGCAG 1 cut(s) 821
BshFI GGCC 2 cut(s) 187, 1166
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 131
BsmAI GTCTC 3 cut(s) 62, 562, 1210
BsmBI CGTCTC 2 cut(s) 62, 562
BsnI GGCC 2 cut(s) 187, 1166
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 5 cut(s) 121, 202, 274, 651, 832
BspACI CCGC 4 cut(s) 552, 889, 911, 1076
BspANI GGCC 2 cut(s) 187, 1166
BspCNI CTCAG 3 cut(s) 583, 616, 700
BspLI GGNNCC 2 cut(s) 544, 909
BspPI GGATC 4 cut(s) 116, 197, 282, 646
BspTI CTTAAG 1 cut(s) 400
BsrDI GCAATG 2 cut(s) 375, 757
BsrI ACTGG 2 cut(s) 6, 776
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 5 cut(s) 121, 202, 274, 651, 832
BssNAI GTATAC 1 cut(s) 75
Bst1107I GTATAC 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 181, 189, 905
Bst4CI ACNGT 3 cut(s) 117, 524, 542
Bst6I CTCTTC 1 cut(s) 674
BstAFI CTTAAG 1 cut(s) 400
BstAPI GCANNNNNTGC 1 cut(s) 46
BstDEI CTNAG 4 cut(s) 570, 603, 687, 1174
BstF5I GGATG 3 cut(s) 91, 845, 1084
BstKTI GATC 5 cut(s) 124, 205, 277, 654, 835
BstMAI GTCTC 3 cut(s) 62, 562, 1210
BstMBI GATC 5 cut(s) 121, 202, 274, 651, 832
BstMWI GCNNNNNNNGC 2 cut(s) 46, 697
BstNI CCWGG 3 cut(s) 181, 189, 905
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 3 cut(s) 179, 187, 903
BstSFI CTRYAG 2 cut(s) 783, 1071
BstV1I GCAGC 1 cut(s) 959
BstV2I GAAGAC 2 cut(s) 525, 746
BstZ17I GTATAC 1 cut(s) 75
BsuRI GGCC 2 cut(s) 187, 1166
BtsCI GGATG 3 cut(s) 91, 845, 1084
BtsI GCAGTG 2 cut(s) 695, 1027
BtsIMutI CAGTG 5 cut(s) 13, 529, 654, 695, 1027
Csp6I GTAC 2 cut(s) 27, 1206
CviAII CATG 3 cut(s) 94, 140, 988
CviQI GTAC 2 cut(s) 27, 1206
DdeI CTNAG 4 cut(s) 570, 603, 687, 1174
DpnI GATC 5 cut(s) 123, 204, 276, 653, 834
DpnII GATC 5 cut(s) 121, 202, 274, 651, 832
EaeI YGGCCR 1 cut(s) 1164
Eam1104I CTCTTC 1 cut(s) 674
EarI CTCTTC 1 cut(s) 674
EciI GGCGGA 1 cut(s) 900
Eco57I CTGAAG 1 cut(s) 192
EcoRI GAATTC 3 cut(s) 134, 362, 395
EcoRII CCWGG 3 cut(s) 179, 187, 903
Esp3I CGTCTC 2 cut(s) 62, 562
FaeI CATG 3 cut(s) 97, 143, 991
FatI CATG 3 cut(s) 93, 139, 987
FauI CCCGC 1 cut(s) 896
FauNDI CATATG 2 cut(s) 613, 1095
FblI GTMKAC 1 cut(s) 74
Fnu4HI GCNGC 1 cut(s) 948
FokI GGATG 3 cut(s) 98, 852, 1091
Fsp4HI GCNGC 1 cut(s) 948
FspBI CTAG 3 cut(s) 632, 963, 1017
GluI GCNGC 1 cut(s) 948
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 2 cut(s) 187, 1166
HapII CCGG 1 cut(s) 131
Hin1II CATG 3 cut(s) 97, 143, 991
HinfI GANTC 3 cut(s) 489, 742, 1051
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 2 cut(s) 75, 1208
Hpy188I TCNGA 5 cut(s) 242, 471, 573, 1006, 1045
Hpy188III TCNNGA 4 cut(s) 320, 389, 441, 1055
Hpy8I GTNNAC 2 cut(s) 75, 1208
HpyAV CCTTC 4 cut(s) 216, 947, 993, 1001
HpyCH4III ACNGT 3 cut(s) 117, 524, 542
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 7 cut(s) 40, 435, 750, 802, 848, 947, 1036
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 697
HpyF3I CTNAG 4 cut(s) 570, 603, 687, 1174
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 3 cut(s) 97, 143, 991
Kzo9I GATC 5 cut(s) 121, 202, 274, 651, 832
Lsp1109I GCAGC 1 cut(s) 959
LweI GCATC 3 cut(s) 222, 703, 1057
MaeI CTAG 3 cut(s) 632, 963, 1017
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 5 cut(s) 536, 693, 761, 778, 1105
MalI GATC 5 cut(s) 123, 204, 276, 653, 834
MboI GATC 5 cut(s) 121, 202, 274, 651, 832
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 1 cut(s) 1166
MluCI AATT 9 cut(s) 134, 362, 395, 474, 656, 766, 879, 1025, 1156
MluNI TGGCCA 1 cut(s) 1166
MlyI GAGTC 2 cut(s) 498, 736
MmeI TCCRAC 1 cut(s) 656
MnlI CCTC 4 cut(s) 217, 439, 579, 1051
Mox20I TGGCCA 1 cut(s) 1166
MscI TGGCCA 1 cut(s) 1166
MseI TTAA 3 cut(s) 51, 309, 401
MslI CAYNNNNRTG 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 1166
MspA1I CMGCKG 1 cut(s) 691
MspCI CTTAAG 1 cut(s) 400
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 3 cut(s) 181, 189, 905
MvaI CCWGG 3 cut(s) 181, 189, 905
MwoI GCNNNNNNNGC 2 cut(s) 46, 697
NdeI CATATG 2 cut(s) 613, 1095
NdeII GATC 5 cut(s) 121, 202, 274, 651, 832
NlaIII CATG 3 cut(s) 97, 143, 991
NlaIV GGNNCC 2 cut(s) 544, 909
NmuCI GTSAC 4 cut(s) 536, 693, 761, 1105
NspI RCATGY 1 cut(s) 97
PciI ACATGT 1 cut(s) 93
PfeI GAWTC 1 cut(s) 1051
PkrI GCNGC 1 cut(s) 949
PleI GAGTC 2 cut(s) 497, 736
PpsI GAGTC 2 cut(s) 497, 736
PscI ACATGT 1 cut(s) 93
Psp6I CCWGG 3 cut(s) 179, 187, 903
PspGI CCWGG 3 cut(s) 179, 187, 903
PspN4I GGNNCC 2 cut(s) 544, 909
PvuII CAGCTG 1 cut(s) 691
RsaI GTAC 2 cut(s) 28, 1207
RsaNI GTAC 2 cut(s) 27, 1206
RseI CAYNNNNRTG 1 cut(s) 144
SaqAI TTAA 3 cut(s) 51, 309, 401
SatI GCNGC 1 cut(s) 948
Sau3AI GATC 5 cut(s) 121, 202, 274, 651, 832
SchI GAGTC 2 cut(s) 498, 736
ScrFI CCNGG 3 cut(s) 181, 189, 905
SduI GDGCHC 1 cut(s) 388
SfaNI GCATC 3 cut(s) 222, 703, 1057
SfcI CTRYAG 2 cut(s) 783, 1071
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 400
SmoI CTYRAG 1 cut(s) 400
Sse9I AATT 9 cut(s) 134, 362, 395, 474, 656, 766, 879, 1025, 1156
SsiI CCGC 4 cut(s) 552, 889, 911, 1076
SspI AATATT 3 cut(s) 55, 1088, 1198
SspMI CTAG 3 cut(s) 632, 963, 1017
StyD4I CCNGG 3 cut(s) 179, 187, 903
TaaI ACNGT 3 cut(s) 117, 524, 542
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 218
TasI AATT 9 cut(s) 134, 362, 395, 474, 656, 766, 879, 1025, 1156
TatI WGTACW 1 cut(s) 1205
TfiI GAWTC 1 cut(s) 1051
Tru1I TTAA 3 cut(s) 51, 309, 401
Tru9I TTAA 3 cut(s) 51, 309, 401
TscAI CASTG 5 cut(s) 13, 529, 654, 702, 1027
TseFI GTSAC 4 cut(s) 536, 693, 761, 1105
TseI GCWGC 1 cut(s) 947
Tsp45I GTSAC 4 cut(s) 536, 693, 761, 1105
TspDTI ATGAA 6 cut(s) 204, 309, 600, 878, 1095, 1128
TspGWI ACGGA 2 cut(s) 208, 719
TspRI CASTG 5 cut(s) 13, 529, 654, 702, 1027
Vha464I CTTAAG 1 cut(s) 400
XapI RAATTY 4 cut(s) 134, 362, 395, 1156
XceI RCATGY 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 74
XspI CTAG 3 cut(s) 632, 963, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.