RchiOBHm_Chr3g0468721

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002940
Physical Location & Seq
Forward (+)
14659369 .. 14661354
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43459

Sequence Viewer

Length: 1596 bp
ATGGATACTGACTGTGGAGCTGAATTTGGTGAATGGTTGGTTTTTGATGCTAAGGATAGTTGTAAGAATGTTGAAAGTTCGTTAATTTCGGATAAAGATGGAGTAAAAGATGAGACTTTGAGGGAAAGGATGGAAGGTAAAGTTGTGAGTGCTCTGGGAGTGGAATTTACTAATAACATTGAGTCTTTGGATTATCAAGACTTCAATGCTAGCGGCTGTTTTAAGACGGTAAATTGTGATGGAGTTGAAAATGAGGCCTTGGAAGTTAATGTGGAAGAAGAGAGTAATCGAAATGAATTGACGCCGATGAGGGTCCCTGTGAGTACTGAATGCGAGGTTGATTTGCTCAGTGTTGCTGATTTTATCCGGTATTCGAGGATGGACGGGAATGTGTCAGGTGGTGTGAATGGAAAATCTTGTTCTTCCAAGCTAGAATTTGAAGTTTCTGATCTAGTATGGGGCAAAGTAAGGACTCATCCATGGTGGCCCGGGCAGATTTGTGATCCTTCGGCTGCGTCAGAACTGGCAAAGAAGTGTTTTAAGAGGGGTAGTTATCTGATAGCTTACTTTGGGGATCAGACATTTGCTTGGAATGAAGCACCAAAGATTAAGCCCTTTCTGGAGCACTGCTCAAAAATGGAGAAGCAGAGTGATATGGAAGAATTCCAAAACGCAGTTGGTTGTGCGTTGGACGAAGTGTCGAGAAGGGTAGAGTTTGGGCTGGCCTGTTCTTGCATATCCAAACGAGAATATGCAAAGCTCAAAACTCAGAAGATTATTAATGCTGGAATCAGGAAAGAAGCAGCAAATAGAAGAGATGCCGGTGACAGTTCTCTTAGCGCCGCTTTTTTTAAACCTGCGAAAGTCATTAAGTTTATAAAAAAGTTGGCTCAGTTGCCATATGGCAAAGCTGACAGACTAGAACTTGTAACATCTCTAGCCCAGCTGTCAGCTTTCTTGCGTTGGAAGAGTTATGTGCCTCTGCCAGAGGAGAATGATACTGACATTTTCCTATTGGGAGAGAAGAACCACTGCAATGAAGTAGGTGATGAAGCACTCTCAGTACTTGAGAATGAGAAGTTGGTGCAAAAATCAGATAGTAAAGAGAACTCATTACGTGAGTGCAAGGACTTATCAGATTCAAAAGCTCAGAGGTGTTTGTTTCCTATGAGTGAGAATGGATCAGAAGGGAAAGCTGGCGGTGAATTGATTTTGCAACATTGCAACAAAAGAAGGGGTCTTGATGCTATAGCTGATGAAGATGTACTCCCAGTAATCAAAGATAACTCCATGCATAAGTGTCGGCACATATCTGAACATAGTATGAATCTGAGTGAAGAAGAGCAAAGCTTGTCAGATTTGGAGGGTACGAAGTGTTTGTCTACTCCAATGAGTGAGAATGGATTAGAAGAGAAAGGTGATGGTAAATTGATTTCACAACCTTCTGTTAAGAAACGAAAGGAAGTTGATGCTATGGCTGATGATGCTGCTGTGAAACATAGGAAAAGTGATTCATCACAAGGTATTGGTAGCAATGCGAACTGTCAGTTGTTTGAAGTTGAAGACTTTTTTAACCTGTTTTTACAGCAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

531

Amino Acids

59.11

Weight (kDa)

5.0

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 150 - 232 1.5e-18 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 878
Acc36I ACCTGC 1 cut(s) 865
AccI GTMKAC 1 cut(s) 1382
AciI CCGC 3 cut(s) 213, 843, 1200
AclWI GGATC 3 cut(s) 497, 582, 1189
AcsI RAATTY 4 cut(s) 23, 164, 434, 662
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 4 cut(s) 325, 1065, 1266, 1369
AgsI TTSAA 7 cut(s) 74, 205, 248, 440, 1143, 1556, 1562
AhdI GACNNNNNGTC 1 cut(s) 697
AjuI GAANNNNNNNTTGG 2 cut(s) 1064, 1096
Alw21I GWGCWC 2 cut(s) 154, 627
Alw26I GTCTC 1 cut(s) 107
AlwI GGATC 3 cut(s) 497, 582, 1189
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 3 cut(s) 255, 485, 723
ApeKI GCWGC 3 cut(s) 512, 803, 1487
ApoI RAATTY 4 cut(s) 23, 164, 434, 662
ArsI GACNNNNNNTTYG 2 cut(s) 1364, 1396
AseI ATTAAT 1 cut(s) 780
AspLEI GCGC 1 cut(s) 842
AspS9I GGNCC 2 cut(s) 313, 486
AsuC2I CCSGG 2 cut(s) 489, 490
AsuHPI GGTGA 5 cut(s) 41, 836, 1058, 1214, 1430
AsuNHI GCTAGC 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 1569
Bbv12I GWGCWC 2 cut(s) 154, 627
BbvI GCAGC 3 cut(s) 499, 815, 1474
BccI CCATC 5 cut(s) 92, 124, 233, 373, 1415
BcgI CGANNNNNNTGC 2 cut(s) 1283, 1317
BcnI CCSGG 2 cut(s) 489, 490
BcoDI GTCTC 1 cut(s) 107
BfaI CTAG 5 cut(s) 210, 431, 452, 920, 938
BfmI CTRYAG 1 cut(s) 1248
BfoI RGCGCY 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 865
BisI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
BlsI GCNGC 5 cut(s) 215, 514, 805, 844, 1489
BmcAI AGTACT 2 cut(s) 325, 1065
Bme1390I CCNGG 2 cut(s) 489, 490
Bme18I GGWCC 1 cut(s) 313
BmeRI GACNNNNNGTC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 2 cut(s) 313, 486
BmiI GGNNCC 2 cut(s) 314, 315
BmrFI CCNGG 2 cut(s) 489, 490
BmrI ACTGGG 1 cut(s) 1265
BmsI GCATC 5 cut(s) 37, 808, 1234, 1459, 1474
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 1265
BpiI GAAGAC 1 cut(s) 1569
BplI GAGNNNNNCTC 2 cut(s) 614, 646
BpmI CTGGAG 1 cut(s) 641
Bpu10I CCTNAGC 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 2 cut(s) 489, 490
BsaAI YACGTR 1 cut(s) 1118
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 258, 479, 488
BsaWI WCCGGW 1 cut(s) 366
Bse118I RCCGGY 1 cut(s) 821
Bse1I ACTGG 2 cut(s) 528, 1271
Bse3DI GCAATG 3 cut(s) 1042, 1219, 1540
BseDI CCNNGG 3 cut(s) 258, 479, 488
BseGI GGATG 3 cut(s) 135, 384, 475
BseMI GCAATG 3 cut(s) 1042, 1219, 1540
BseMII CTCAG 6 cut(s) 361, 782, 905, 1074, 1163, 1322
BseNI ACTGG 2 cut(s) 528, 1271
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 3 cut(s) 499, 815, 1474
BseYI CCCAGC 1 cut(s) 942
BshFI GGCC 3 cut(s) 257, 487, 725
BsiHKAI GWGCWC 2 cut(s) 154, 627
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 367, 489, 822
BslFI GGGAC 1 cut(s) 299
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 299
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 3 cut(s) 257, 487, 725
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 154, 627
Bsp143I GATC 4 cut(s) 448, 502, 574, 1181
Bsp19I CCATGG 1 cut(s) 479
BspACI CCGC 3 cut(s) 213, 843, 1200
BspANI GGCC 3 cut(s) 257, 487, 725
BspCNI CTCAG 6 cut(s) 360, 781, 904, 1073, 1162, 1323
BspLI GGNNCC 2 cut(s) 314, 315
BspMI ACCTGC 1 cut(s) 865
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 497, 582, 1189
BspQI GCTCTTC 1 cut(s) 1335
BsrDI GCAATG 3 cut(s) 1042, 1219, 1540
BsrFI RCCGGY 1 cut(s) 821
BsrI ACTGG 2 cut(s) 528, 1271
BssAI RCCGGY 1 cut(s) 821
BssECI CCNNGG 3 cut(s) 258, 479, 488
BssMI GATC 4 cut(s) 448, 502, 574, 1181
BssNI GRCGYC 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 258, 479
Bst4CI ACNGT 4 cut(s) 14, 229, 830, 1544
Bst6I CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
BstACI GRCGYC 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 1118
BstC8I GCNNGC 3 cut(s) 211, 723, 1198
BstDEI CTNAG 8 cut(s) 51, 347, 768, 836, 891, 1060, 1149, 1331
BstDSI CCRYGG 1 cut(s) 479
BstF5I GGATG 3 cut(s) 135, 384, 475
BstH2I RGCGCY 1 cut(s) 843
BstHHI GCGC 1 cut(s) 842
BstKTI GATC 4 cut(s) 451, 505, 577, 1184
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 4 cut(s) 448, 502, 574, 1181
BstMWI GCNNNNNNNGC 1 cut(s) 1484
BstSCI CCNGG 2 cut(s) 487, 488
BstSFI CTRYAG 1 cut(s) 1248
BstV1I GCAGC 3 cut(s) 499, 815, 1474
BstV2I GAAGAC 1 cut(s) 1569
BsuRI GGCC 3 cut(s) 257, 487, 725
BtgI CCRYGG 1 cut(s) 479
BtsCI GGATG 3 cut(s) 135, 384, 475
BtsI GCAGTG 2 cut(s) 625, 1030
BtsIMutI CAGTG 3 cut(s) 355, 625, 1030
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 3 cut(s) 211, 723, 1198
CfoI GCGC 1 cut(s) 842
Cfr10I RCCGGY 1 cut(s) 821
Cfr13I GGNCC 2 cut(s) 313, 486
Cfr9I CCCGGG 1 cut(s) 488
CseI GACGC 2 cut(s) 310, 504
Csp6I GTAC 4 cut(s) 324, 1064, 1265, 1368
CviAII CATG 2 cut(s) 480, 1291
CviQI GTAC 4 cut(s) 324, 1064, 1265, 1368
DdeI CTNAG 8 cut(s) 51, 347, 768, 836, 891, 1060, 1149, 1331
DpnI GATC 4 cut(s) 450, 504, 576, 1183
DpnII GATC 4 cut(s) 448, 502, 574, 1181
DraI TTTAAA 1 cut(s) 853
DriI GACNNNNNGTC 1 cut(s) 697
Eam1104I CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
Eam1105I GACNNNNNGTC 1 cut(s) 697
EarI CTCTTC 5 cut(s) 273, 808, 962, 1335, 1404
Eco130I CCWWGG 2 cut(s) 258, 479
Eco147I AGGCCT 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 662
EcoT14I CCWWGG 2 cut(s) 258, 479
EcoT22I ATGCAT 1 cut(s) 1296
ErhI CCWWGG 2 cut(s) 258, 479
FaeI CATG 2 cut(s) 483, 1294
FaqI GGGAC 1 cut(s) 299
FatI CATG 2 cut(s) 479, 1290
FauNDI CATATG 1 cut(s) 901
FblI GTMKAC 1 cut(s) 1382
Fnu4HI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
FokI GGATG 3 cut(s) 142, 391, 462
Fsp4HI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
FspBI CTAG 5 cut(s) 210, 431, 452, 920, 938
GlaI GCGC 1 cut(s) 841
GluI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 1 cut(s) 641
HaeII RGCGCY 1 cut(s) 843
HaeIII GGCC 3 cut(s) 257, 487, 725
HapII CCGG 3 cut(s) 367, 489, 822
HgaI GACGC 2 cut(s) 310, 504
HhaI GCGC 1 cut(s) 842
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 2 cut(s) 483, 1294
Hin6I GCGC 1 cut(s) 840
HinP1I GCGC 1 cut(s) 840
HindIII AAGCTT 1 cut(s) 1348
HinfI GANTC 6 cut(s) 182, 472, 789, 1139, 1327, 1511
HpaII CCGG 3 cut(s) 367, 489, 822
HphI GGTGA 5 cut(s) 41, 836, 1058, 1214, 1430
Hpy166II GTNNAC 1 cut(s) 1383
Hpy188III TCNNGA 5 cut(s) 197, 620, 702, 793, 1241
Hpy8I GTNNAC 1 cut(s) 1383
HpyAV CCTTC 6 cut(s) 128, 516, 699, 1181, 1227, 1452
HpyCH4III ACNGT 4 cut(s) 14, 229, 830, 1544
HpyCH4IV ACGT 1 cut(s) 1117
HpyCH4V TGCA 8 cut(s) 735, 755, 1035, 1087, 1125, 1216, 1224, 1294
HpyF10VI GCNNNNNNNGC 1 cut(s) 1484
HpyF3I CTNAG 8 cut(s) 51, 347, 768, 836, 891, 1060, 1149, 1331
HpySE526I ACGT 1 cut(s) 1117
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 2 cut(s) 483, 1294
HspAI GCGC 1 cut(s) 840
KflI GGGWCCC 1 cut(s) 313
Kzo9I GATC 4 cut(s) 448, 502, 574, 1181
LguI GCTCTTC 1 cut(s) 1335
LmnI GCTCC 2 cut(s) 17, 622
Lsp1109I GCAGC 3 cut(s) 499, 815, 1474
LweI GCATC 5 cut(s) 37, 808, 1234, 1459, 1474
MaeI CTAG 5 cut(s) 210, 431, 452, 920, 938
MaeII ACGT 1 cut(s) 1117
MaeIII GTNAC 2 cut(s) 824, 928
MalI GATC 4 cut(s) 450, 504, 576, 1183
MboI GATC 4 cut(s) 448, 502, 574, 1181
MhlI GDGCHC 2 cut(s) 154, 627
MluCI AATT 9 cut(s) 23, 84, 164, 232, 296, 434, 662, 1205, 1427
MlyI GAGTC 2 cut(s) 191, 466
MmeI TCCRAC 2 cut(s) 669, 944
Mph1103I ATGCAT 1 cut(s) 1296
MslI CAYNNNNRTG 1 cut(s) 1035
MspA1I CMGCKG 1 cut(s) 946
MspI CCGG 3 cut(s) 367, 489, 822
MspR9I CCNGG 2 cut(s) 489, 490
Mva1269I GAATGC 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 1484
NciI CCSGG 2 cut(s) 489, 490
NcoI CCATGG 1 cut(s) 479
NdeI CATATG 1 cut(s) 901
NdeII GATC 4 cut(s) 448, 502, 574, 1181
NheI GCTAGC 1 cut(s) 209
NlaIII CATG 2 cut(s) 483, 1294
NlaIV GGNNCC 2 cut(s) 314, 315
NmuCI GTSAC 1 cut(s) 824
NsiI ATGCAT 1 cut(s) 1296
PceI AGGCCT 1 cut(s) 257
PciSI GCTCTTC 1 cut(s) 1335
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 4 cut(s) 789, 1139, 1327, 1511
PkrI GCNGC 5 cut(s) 215, 514, 805, 844, 1489
PleI GAGTC 2 cut(s) 190, 466
PpsI GAGTC 2 cut(s) 190, 466
Ppu21I YACGTR 1 cut(s) 1118
PpuMI RGGWCCY 1 cut(s) 313
PshBI ATTAAT 1 cut(s) 780
PsiI TTATAA 1 cut(s) 878
Psp5II RGGWCCY 1 cut(s) 313
PspFI CCCAGC 1 cut(s) 942
PspN4I GGNNCC 2 cut(s) 314, 315
PspPI GGNCC 2 cut(s) 313, 486
PspPPI RGGWCCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 946
RsaI GTAC 4 cut(s) 325, 1065, 1266, 1369
RsaNI GTAC 4 cut(s) 324, 1064, 1265, 1368
RseI CAYNNNNRTG 1 cut(s) 1035
SapI GCTCTTC 1 cut(s) 1335
SatI GCNGC 5 cut(s) 214, 513, 804, 843, 1488
Sau3AI GATC 4 cut(s) 448, 502, 574, 1181
Sau96I GGNCC 2 cut(s) 313, 486
ScaI AGTACT 2 cut(s) 325, 1065
SchI GAGTC 2 cut(s) 191, 466
ScrFI CCNGG 2 cut(s) 489, 490
SduI GDGCHC 2 cut(s) 154, 627
SfaNI GCATC 5 cut(s) 37, 808, 1234, 1459, 1474
SfcI CTRYAG 1 cut(s) 1248
SinI GGWCC 1 cut(s) 313
SmaI CCCGGG 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 1035
SmlI CTYRAG 1 cut(s) 1067
SmoI CTYRAG 1 cut(s) 1067
SrfI GCCCGGGC 1 cut(s) 490
Sse9I AATT 9 cut(s) 23, 84, 164, 232, 296, 434, 662, 1205, 1427
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 3 cut(s) 213, 843, 1200
SspMI CTAG 5 cut(s) 210, 431, 452, 920, 938
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 2 cut(s) 487, 488
StyI CCWWGG 2 cut(s) 258, 479
TaaI ACNGT 4 cut(s) 14, 229, 830, 1544
TaiI ACGT 1 cut(s) 1120
TaqI TCGA 3 cut(s) 289, 374, 701
TasI AATT 9 cut(s) 23, 84, 164, 232, 296, 434, 662, 1205, 1427
TatI WGTACW 3 cut(s) 323, 1063, 1264
TauI GCSGC 2 cut(s) 216, 845
TfiI GAWTC 4 cut(s) 789, 1139, 1327, 1511
TscAI CASTG 3 cut(s) 355, 632, 1037
TseFI GTSAC 1 cut(s) 824
TseI GCWGC 3 cut(s) 512, 803, 1487
Tsp45I GTSAC 1 cut(s) 824
TspDTI ATGAA 7 cut(s) 309, 609, 1053, 1065, 1272, 1340, 1503
TspMI CCCGGG 1 cut(s) 488
TspRI CASTG 3 cut(s) 355, 632, 1037
VpaK11BI GGWCC 1 cut(s) 313
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 4 cut(s) 23, 164, 434, 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmaI CCCGGG 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 1382
XspI CTAG 5 cut(s) 210, 431, 452, 920, 938
ZrmI AGTACT 2 cut(s) 325, 1065
Zsp2I ATGCAT 1 cut(s) 1296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.