Rh3BG168300

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002940
Physical Location & Seq
Forward (+)
14471726 .. 14472640
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG168300.1

Sequence Viewer

Length: 915 bp
ATGGATGGGAACATGTTTAGTGATGTGAATGGCCAATATGATCCAAAACCGGAATTCCATGTTTATGATATAGTATGGGGGAAAGTAAGGGGTTATCCGTGGTGGCCTGGACGGATATGTGATCCTTCAGCTTCATCGAATGAGGCAAAGATGCTTTTCAGAAAGGGTAGTTATTTGATAGATTATTTTGGGGATCACTCATTTACTTGGAATGAAGCACCACATATTAAGCCCTTTCTGGATTATTTCTCGCAAATGGAGAAGCAGAGTAACAAGGACGAATTCCAAAATGCCATTGCTTGTGCTCTGGACGAATTCTTAAGACGGGTAGAGTTGGGGGTGGTCTGTTCTTGCATATCTGGAGAGGTATATCCAAAACTCAAAAATCAGATAATTATTAGTGATGGAGTCAGGCAAGAAGAAGAAGAAGAAGAAGAAGAAGACAGTGGAAATGCTGGTGACGGTTCCCTTACCGCCATCTCTTTTGAACCTCAGAAACTCATTATGCTTCTAAAGGAGTTTGCTCAGTTTTCATATGGAAAAGCTGATCAACTAGAACTTTCAACATCAGTGATCCAATTATCAGCTTTCTATTGTTGGAAGAGTTCTCAGCTGTCGCTGCCAACAGAGAATGATGCCTATATATCAGTATTGGGGGAGAAGACTCAATTCAATGTATTAGGTCACAATTCACTTCCAGTAACTACAGATGAGAAGCTGGTGCAGAAATCTAAAAGTAAAGGTAACTATAATAACATATCTGAGGATAATAGTCTGGGTAAAGAACAGAAAAGTTTGTCAGATTTAACGGCTCAGAAGTGTTTCTCTGGGGGGAGGGAGGCACAATGGCACAAGGATATGCCACTGCACCCCCTGACACTTACTACTGTTCTATTTTGGTTACTTATACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

304

Amino Acids

34.48

Weight (kDa)

4.87

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 23 - 105 2.6e-19 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 474
AclWI GGATC 4 cut(s) 35, 116, 201, 568
AcoI YGGCCR 1 cut(s) 31
AcsI RAATTY 3 cut(s) 53, 281, 314
AcuI CTGAAG 1 cut(s) 111
AflII CTTAAG 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 12
AgsI TTSAA 3 cut(s) 488, 564, 673
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 5 cut(s) 131, 545, 587, 613, 718
AluI AGCT 5 cut(s) 131, 545, 587, 613, 718
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 4 cut(s) 35, 116, 201, 568
AoxI GGCC 2 cut(s) 31, 104
ApeKI GCWGC 1 cut(s) 619
ApoI RAATTY 3 cut(s) 53, 281, 314
Asp700I GAANNNNTTC 1 cut(s) 821
AsuHPI GGTGA 1 cut(s) 470
BalI TGGCCA 1 cut(s) 33
BbsI GAAGAC 2 cut(s) 447, 668
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 606
BccI CCATC 2 cut(s) 398, 485
BceAI ACGGC 1 cut(s) 825
BciT130I CCWGG 1 cut(s) 108
BclI TGATCA 1 cut(s) 547
BfaI CTAG 1 cut(s) 554
BfmI CTRYAG 1 cut(s) 705
BfrI CTTAAG 1 cut(s) 319
BisI GCNGC 1 cut(s) 620
BlsI GCNGC 1 cut(s) 621
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 141, 625
BpiI GAAGAC 2 cut(s) 447, 668
BpmI CTGGAG 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 698
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 98
BseGI GGATG 1 cut(s) 10
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 5 cut(s) 506, 539, 623, 753, 827
BseNI ACTGG 1 cut(s) 698
BseXI GCAGC 1 cut(s) 606
BsgI GTGCAG 2 cut(s) 743, 851
BshFI GGCC 2 cut(s) 33, 106
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 50
BsnI GGCC 2 cut(s) 33, 106
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 5 cut(s) 40, 121, 193, 547, 573
BspACI CCGC 1 cut(s) 474
BspANI GGCC 2 cut(s) 33, 106
BspCNI CTCAG 5 cut(s) 505, 538, 622, 754, 826
BspLI GGNNCC 1 cut(s) 466
BspPI GGATC 4 cut(s) 35, 116, 201, 568
BspTI CTTAAG 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 1 cut(s) 698
BssECI CCNNGG 1 cut(s) 98
BssMI GATC 5 cut(s) 40, 121, 193, 547, 573
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 446, 464, 889
Bst6I CTCTTC 1 cut(s) 596
BstAFI CTTAAG 1 cut(s) 319
BstDEI CTNAG 5 cut(s) 492, 525, 609, 762, 813
BstDSI CCRYGG 1 cut(s) 98
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 5 cut(s) 43, 124, 196, 550, 576
BstMBI GATC 5 cut(s) 40, 121, 193, 547, 573
BstMWI GCNNNNNNNGC 1 cut(s) 619
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 705
BstV1I GCAGC 1 cut(s) 606
BstV2I GAAGAC 2 cut(s) 447, 668
BsuRI GGCC 2 cut(s) 33, 106
BtgI CCRYGG 1 cut(s) 98
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 863
BtsIMutI CAGTG 3 cut(s) 451, 576, 863
CviAII CATG 2 cut(s) 13, 59
CviJI RGCY 9 cut(s) 33, 106, 131, 232, 545, 587, 613, 718, 812
CviKI_1 RGCY 9 cut(s) 33, 106, 131, 232, 545, 587, 613, 718, 812
DdeI CTNAG 5 cut(s) 492, 525, 609, 762, 813
DpnI GATC 5 cut(s) 42, 123, 195, 549, 575
DpnII GATC 5 cut(s) 40, 121, 193, 547, 573
EaeI YGGCCR 1 cut(s) 31
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 111
EcoRI GAATTC 3 cut(s) 53, 281, 314
EcoRII CCWGG 1 cut(s) 106
FaeI CATG 2 cut(s) 16, 62
FatI CATG 2 cut(s) 12, 58
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 620
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 620
FspBI CTAG 1 cut(s) 554
GluI GCNGC 1 cut(s) 620
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 2 cut(s) 33, 106
HapII CCGG 1 cut(s) 50
Hin1II CATG 2 cut(s) 16, 62
HinfI GANTC 2 cut(s) 408, 664
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 470
Hpy188I TCNGA 6 cut(s) 161, 390, 495, 763, 802, 816
Hpy188III TCNNGA 3 cut(s) 239, 308, 360
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 3 cut(s) 446, 464, 889
HpyCH4V TGCA 3 cut(s) 354, 724, 868
HpyF10VI GCNNNNNNNGC 1 cut(s) 619
HpyF3I CTNAG 5 cut(s) 492, 525, 609, 762, 813
Hsp92II CATG 2 cut(s) 16, 62
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 5 cut(s) 40, 121, 193, 547, 573
Lsp1109I GCAGC 1 cut(s) 606
LweI GCATC 2 cut(s) 141, 625
MaeI CTAG 1 cut(s) 554
MaeIII GTNAC 6 cut(s) 269, 458, 683, 700, 743, 900
MalI GATC 5 cut(s) 42, 123, 195, 549, 575
MboI GATC 5 cut(s) 40, 121, 193, 547, 573
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 33
MluCI AATT 7 cut(s) 53, 281, 314, 393, 578, 668, 688
MluNI TGGCCA 1 cut(s) 33
MlyI GAGTC 2 cut(s) 417, 658
MmeI TCCRAC 1 cut(s) 578
MnlI CCTC 6 cut(s) 136, 358, 501, 757, 828, 832
Mox20I TGGCCA 1 cut(s) 33
MroXI GAANNNNTTC 1 cut(s) 821
MscI TGGCCA 1 cut(s) 33
MseI TTAA 3 cut(s) 228, 320, 806
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 613
MspCI CTTAAG 1 cut(s) 319
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 619
NdeI CATATG 1 cut(s) 535
NdeII GATC 5 cut(s) 40, 121, 193, 547, 573
NlaIII CATG 2 cut(s) 16, 62
NlaIV GGNNCC 1 cut(s) 466
NmuCI GTSAC 2 cut(s) 458, 683
NspI RCATGY 1 cut(s) 16
PciI ACATGT 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 821
PkrI GCNGC 1 cut(s) 621
PleI GAGTC 2 cut(s) 416, 658
PpsI GAGTC 2 cut(s) 416, 658
PscI ACATGT 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 613
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 3 cut(s) 228, 320, 806
SatI GCNGC 1 cut(s) 620
Sau3AI GATC 5 cut(s) 40, 121, 193, 547, 573
SchI GAGTC 2 cut(s) 417, 658
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 2 cut(s) 141, 625
SfcI CTRYAG 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 7 cut(s) 53, 281, 314, 393, 578, 668, 688
SsiI CCGC 1 cut(s) 474
SspMI CTAG 1 cut(s) 554
StyD4I CCNGG 1 cut(s) 106
TaaI ACNGT 3 cut(s) 446, 464, 889
TaqI TCGA 1 cut(s) 137
TasI AATT 7 cut(s) 53, 281, 314, 393, 578, 668, 688
Tru1I TTAA 3 cut(s) 228, 320, 806
Tru9I TTAA 3 cut(s) 228, 320, 806
TscAI CASTG 3 cut(s) 451, 576, 870
TseFI GTSAC 2 cut(s) 458, 683
TseI GCWGC 1 cut(s) 619
Tsp45I GTSAC 2 cut(s) 458, 683
TspDTI ATGAA 3 cut(s) 123, 228, 522
TspGWI ACGGA 2 cut(s) 87, 127
TspRI CASTG 3 cut(s) 451, 576, 870
Vha464I CTTAAG 1 cut(s) 319
XapI RAATTY 3 cut(s) 53, 281, 314
XceI RCATGY 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 821
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.