Rh3BG168800

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002940
Physical Location & Seq
Forward (+)
14496406 .. 14500548
4143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG168800.1

Sequence Viewer

Length: 3372 bp
ATGGCTCGGATAAGAAAGCACGGGGCTAATTTCTCTGGGGAGGGAGGAGTTGAGAGCGTTGATGATAGTGATGCAGTAGTGGGTGAGGACATGAGTGAGGGTTTGGAAGAGGGAGATGAATTGGTAGATGGAGGTGGTGATTCTGGAGGGAAAGTAGGACCTGTAGATATGGAGATTGATGAGAAAGGTGGAGATGGAGTGACAGAGGGTTTGGAGGGGACTCAATTTGGGGGTACTGATGCGAATGCAGCGAGTGAGAAAGGGTCGGTTTCGGAATCGAAGGATGGTTCTAAGGGAGTTGAAAGTTCAGTCCTTAGTGACAAGGATAGGGGTAAAGATGAGATTGTAAGGGAATGCGTGGAACCTGTAGCTGTGGGTACTGTGGAAGGGAACCTCTCGATTAGTTTTGAGGCGAAGGAGGATGGAACAGTTGAGGGAAAGGATGAATTGATGAGAGATGACAAAGAAGAGGAAGTTGCACAAGTAGATGAGGAAGAGAGAGAAAGAGAACAGAATGTGGAGTGTAGCCAGAGTAGCCATGCTACAAGTTCTAACGTGGAGCATGAGGCTACAGGGTCTGTTGGAGAAATACCGCAGGACATCCCAGCTGTGGAAGTGAAAATTGCCGGTATCGATGAGTCTGGGCATGATGGAGACCTGAATGCAAGCACTGGTTCCCAGATGGTTATGTCTGGTGGACTAGAAAATCAGACGTTGGAAGTTAATGTGGGAGTAAAGACCGGTGAAGATGTCTTGATGCCTGAATATGTTTCCCCAGCAGAGGTTTCTGTGAAATCTGAAAATGGGCATAATTTGATTGACATGGCTATTGATTTGGAACCGTATGCTGGAAAGGATGGGAATGTTTCAGATGATAGTAATGTTTCTGGTCCCTCACAACCAGAATTTCAAGCTTTTGATCTAGTATGGGGCAAAATTAGGAGCCATCCATGGTGGCCTGGGCAGATATGTGATCCTTCAGCTTCATCAAAACTTGCAAAGAAGTATTTTAAGAAGCACAGTTATCTGATAGCTTATTTTTGGGATTGTACATTTGCTTGGAATGAAGCACCACAAATTAAGCCCTTTAGGGAGAACTTTTCACAAATGGAAAAGCAGAGCAATATGGAGGAATTCCAAAATGCAGTTGCTTGTGCTCTGGATGAAGTCTCAAGGCGAGTAGAGTTTGGCTTGGCCTGTTCTTGCATTTCCAAAGAAGTTTATGCAAAGCTCAAAACACAGACGATCATTAATGCTGGAATCAAGGAAGAAGCATGTAGAAGAGATGGTGGTGATAGTTCACTGGGTGCTTCTTCTTTTGAACCCTTGAAACTCATTAACTTTGCAAAACAAGCCGCCAAGCTGCCATTTGGCAGAGCTGACAGACTCGAACTTGTTACATCACGGGCCCAATTATCATCTTTCTATCGTTGGAAGGGTTATTCTCAGCTGCCAGAGTTCAATTTTCTTGGTGGGTTGCTGGAAAATGATGCCGACATTTCACTATTGAGAGAAAAGGAGCAATTTAATGAACTACGGGAAGTTGCACTTCCAGTAATTAAGGATGAGGAATTGGAGCAGAAATCAAAAGGGAAAAGCTCCTCATTGCATAAGCGTAAGCACATATCTGGAGATAGTTCCCAACCAGGTAAAAAAGGGAAAAACTCCTCAAATGTAACTGCTGAGAAGGGTTTGTCTACTCCCATGAGTGAGAATGGATCAGAAAGGAAAGCTGACTGTAAGTTGGTTTCACAATCCTCAAGTAAGAAACGAAAGGCAGTTGATGGAGCTATGCCTGATGACTCTGCGGTGAAACATAGTAAAAGCACTTCATCAGCAGTGGAAGATACTGTTCCTTTACAGAATAAGCAGACTTTTAGAGTTGGAGATAGAATCTGTAGGGTTGCAACCCTACTAAATGGGTCAAGTCCAATATTCAGGCACAATGCAGCACCTGCAGATGCTGAAGTTCAGGATGAAGGCAAAGAGAAATCTTCATTGCAGAAACCACAAACTGAAAAGCTGATTGACATGGAGAATCCTTCTCCTGATGAGATGCTGTCTCAGCTTTGCTTGGCTGCCATGAATCCCAATAAAGGGTACAGCATGTTGATTTCCTTGATGGGTTGCTTCTCAAAATTTAGGAATACTATATGCCTGGAACCTCCCAGTCCAGATGAGGATCAATTGCCTTTGGGACAAATATTTGGTAGCAAGCGCAGTAAGAAATCAACCAAGACTGGAAAGAAATCAATCAAACCACGCATCTCTGAAAAATCTGAGTTGGAGCTGGTCAAAGACTCTTATTCTTCTGATAAGATAGTTAAGAGTCAAAATGAAAATGGAGAGTTATTGCTTGGAGCTGCAAGCGATAAAGATACATGTGTTGTGGAGTCACAGTCAAGTCCTGATTCGGAAGCACTGAAGCCAGTTCTTGACAATAAAAATGGAGAGTTGGTGTGTGGAACTGCAACAAGCGATAAGGATACTTCTACTGTTGAACTGCAAGCAAGTCTTGAGTTAAATCCCACTTTGGATTCTGAACAGAACATTGTTTGTGGAGATATTGAATCAAAAGCAGTGAACACAGCTGCTACAAGCGATAAGGATACTTATACTGTTGAACTGCAAGCAACTCTCGAGTTAAATCCCAATTTGGATTCTGAACAGAACATTGTTTATGGAGATATTGATTCAAAAGCAGTGAACCCAGCTCTGGATGATGAAAATGGAGCATTAGTACGTGAGCCCATCTCTGTTGTTGAGTCACCAGCAGATCCCAAGTTAAATCCCAATTTGGATTTTGAACAGGAAAATACAGTAAGAGATACTGGTGCGGAAGCAGTGAAGCCAGCTCTTGACGATGAAAGTGGAGAGTTGGTGGCTGGAGCTCTAAGTAATAAGGATGCTTCCGCTGTGGATTCGCAAGCAAGTCTTGAGTTCAATCCCAATATGGATTCTGCACTGAAATTTCCTGATGGAGATTATGATTCAGAAGCAGTGAAGCCAGCTTCCCCAAGTGAGAGTTGTGAGGAAGATTCTCCCGCAGCTCTGATCTTGAACTTTTCAGACTTGGAGTCTCTTCCCTCGGAAGCAAGTCTAAACAATATTTTTAGCATCTTCGGGCCTCTGAATGAATCGCAAACCGAATTGTTGAAGAAGACAAACCGGGCCAAAGTGGTTTTCAGGCGGCGTTCTGATGCTCAAAAAGCTTTCAGCAGTGCCGGGAAATACAGTACCTTTGGACCTTCACTTGAAAGTTATCGCCTCAAGTATCCGCTATCACCAAGTAAAGCTTCTCCTTCTCCGAAGGTGGCAAAGCAAGGAAAAATGGATGCATCTGCAGAAGGTGATGGAGCTAGAAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

1123

Amino Acids

121.11

Weight (kDa)

4.67

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 307 - 389 2.1e-17 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1963
Acc36I ACCTGC 1 cut(s) 1963
AccI GTMKAC 1 cut(s) 1697
AciI CCGC 8 cut(s) 593, 1356, 1808, 2837, 2913, 3045, 3190, 3278
AclWI GGATC 4 cut(s) 968, 1726, 2190, 2771
AcsI RAATTY 4 cut(s) 905, 1133, 2138, 2969
AcuI CTGAAG 3 cut(s) 963, 1986, 2444
AdeI CACNNNGTG 1 cut(s) 1307
AfaI GTAC 6 cut(s) 235, 379, 1051, 2102, 2742, 3238
AfiI CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2716
AflIII ACRYGT 1 cut(s) 2381
AgeI ACCGGT 1 cut(s) 740
AhdI GACNNNNNGTC 1 cut(s) 3076
AjnI CCWGG 3 cut(s) 958, 1645, 2157
AjuI GAANNNNNNNTTGG 4 cut(s) 3167, 3199, 3280, 3312
Alw21I GWGCWC 2 cut(s) 1159, 2893
Alw26I GTCTC 4 cut(s) 648, 1174, 2067, 3084
AlwI GGATC 4 cut(s) 968, 1726, 2190, 2771
AlwNI CAGNNNCTG 3 cut(s) 578, 1955, 1964
Ama87I CYCGRG 1 cut(s) 2639
AoxI GGCC 5 cut(s) 956, 1194, 1407, 3125, 3171
ApaI GGGCCC 1 cut(s) 1411
ApeKI GCWGC 8 cut(s) 248, 1363, 1450, 1949, 2078, 2363, 2591, 3047
ApoI RAATTY 4 cut(s) 905, 1133, 2138, 2969
ArsI GACNNNNNNTTYG 2 cut(s) 193, 225
AseI ATTAAT 1 cut(s) 1251
AsiGI ACCGGT 1 cut(s) 740
AspLEI GCGC 1 cut(s) 2220
AspS9I GGNCC 7 cut(s) 158, 890, 1407, 1408, 3125, 3171, 3245
AsuC2I CCSGG 2 cut(s) 3170, 3226
AsuHPI GGTGA 8 cut(s) 95, 149, 755, 1304, 1822, 2760, 3276, 3362
AvaI CYCGRG 1 cut(s) 2639
AvaII GGWCC 3 cut(s) 158, 890, 3245
BaeGI GKGCMC 1 cut(s) 1411
BaeI ACNNNNGTAYC 2 cut(s) 2370, 2403
BanII GRGCYC 3 cut(s) 1411, 2751, 2893
BarI GAAGNNNNNNTAC 2 cut(s) 1813, 1845
BbsI GAAGAC 1 cut(s) 3167
Bbv12I GWGCWC 2 cut(s) 1159, 2893
BbvI GCAGC 8 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2578, 3059
BciT130I CCWGG 3 cut(s) 960, 1647, 2159
BciVI GTATCC 3 cut(s) 2479, 2602, 3285
BcnI CCSGG 2 cut(s) 3170, 3226
BcoDI GTCTC 4 cut(s) 648, 1174, 2067, 3084
BfaI CTAG 3 cut(s) 701, 923, 3360
BfmI CTRYAG 6 cut(s) 162, 366, 570, 1897, 1956, 3342
BfuAI ACCTGC 1 cut(s) 1963
BfuI GTATCC 3 cut(s) 2479, 2602, 3285
Bme1390I CCNGG 5 cut(s) 960, 1647, 2159, 3170, 3226
Bme18I GGWCC 3 cut(s) 158, 890, 3245
BmeRI GACNNNNNGTC 1 cut(s) 3076
BmeT110I CYCGRG 1 cut(s) 2639
BmgT120I GGNCC 7 cut(s) 158, 890, 1407, 1408, 3125, 3171, 3245
BmiI GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
BmrFI CCNGG 5 cut(s) 960, 1647, 2159, 3170, 3226
BmrI ACTGGG 2 cut(s) 1313, 2163
BmuI ACTGGG 2 cut(s) 1313, 2163
BpiI GAAGAC 1 cut(s) 3167
BplI GAGNNNNNCTC 2 cut(s) 2738, 2770
BpmI CTGGAG 3 cut(s) 165, 1650, 2907
BpuEI CTTGAG 5 cut(s) 1156, 1744, 2537, 2957, 3254
BpuMI CCSGG 2 cut(s) 3170, 3226
Bsa29I ATCGAT 1 cut(s) 633
BsaAI YACGTR 1 cut(s) 2744
BsaBI GATNNNNATC 1 cut(s) 2181
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 950, 959, 3087
BsaWI WCCGGW 1 cut(s) 740
Bsc4I CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2716
Bse118I RCCGGY 2 cut(s) 626, 740
Bse1I ACTGG 7 cut(s) 676, 1308, 1553, 2169, 2245, 2429, 2836
Bse3DI GCAATG 2 cut(s) 1604, 1997
Bse8I GATNNNNATC 1 cut(s) 2181
BseBI CCWGG 3 cut(s) 960, 1647, 2159
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 3 cut(s) 950, 959, 3087
BseJI GATNNNNATC 1 cut(s) 2181
BseLI CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2716
BseMI GCAATG 2 cut(s) 1604, 1997
BseMII CTCAG 4 cut(s) 1460, 1674, 2078, 2271
BseNI ACTGG 7 cut(s) 676, 1308, 1553, 2169, 2245, 2429, 2836
BseRI GAGGAG 3 cut(s) 60, 1591, 1657
BseSI GKGCMC 1 cut(s) 1411
BseXI GCAGC 8 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2578, 3059
BseYI CCCAGC 3 cut(s) 604, 775, 2710
BsgI GTGCAG 1 cut(s) 2946
BshFI GGCC 5 cut(s) 958, 1196, 1409, 3127, 3173
BshTI ACCGGT 1 cut(s) 740
BshVI ATCGAT 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 1159, 2893
BsiHKCI CYCGRG 1 cut(s) 2639
BsiSI CCGG 4 cut(s) 627, 741, 3169, 3225
BslFI GGGAC 3 cut(s) 232, 876, 2211
BslI CCNNNNNNNGG 6 cut(s) 610, 781, 848, 2096, 2097, 2716
BsmAI GTCTC 4 cut(s) 648, 1174, 2067, 3084
BsmFI GGGAC 3 cut(s) 232, 876, 2211
BsmI GAATGC 3 cut(s) 250, 359, 667
BsnI GGCC 5 cut(s) 958, 1196, 1409, 3127, 3173
Bso31I GGTCTC 1 cut(s) 648
BsoBI CYCGRG 1 cut(s) 2639
Bsp120I GGGCCC 1 cut(s) 1407
Bsp1286I GDGCHC 4 cut(s) 1159, 1411, 2751, 2893
Bsp1407I TGTACA 1 cut(s) 1049
Bsp143I GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 8 cut(s) 593, 1356, 1808, 2837, 2913, 3045, 3190, 3278
BspANI GGCC 5 cut(s) 958, 1196, 1409, 3127, 3173
BspCNI CTCAG 4 cut(s) 1459, 1675, 2077, 2272
BspDI ATCGAT 1 cut(s) 633
BspLI GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
BspMAI CTGCAG 2 cut(s) 1960, 3346
BspMI ACCTGC 1 cut(s) 1963
BspPI GGATC 4 cut(s) 968, 1726, 2190, 2771
BspTNI GGTCTC 1 cut(s) 648
BsrDI GCAATG 2 cut(s) 1604, 1997
BsrFI RCCGGY 2 cut(s) 626, 740
BsrGI TGTACA 1 cut(s) 1049
BsrI ACTGG 7 cut(s) 676, 1308, 1553, 2169, 2245, 2429, 2836
BssAI RCCGGY 2 cut(s) 626, 740
BssECI CCNNGG 3 cut(s) 950, 959, 3087
BssMI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
BssT1I CCWWGG 1 cut(s) 950
Bst2UI CCWGG 3 cut(s) 960, 1647, 2159
Bst6I CTCTTC 5 cut(s) 102, 462, 489, 1276, 3087
BstAPI GCANNNNNTGC 1 cut(s) 1955
BstAUI TGTACA 1 cut(s) 1049
BstBAI YACGTR 1 cut(s) 2744
BstC8I GCNNGC 8 cut(s) 667, 2216, 2368, 2508, 2631, 2853, 2928, 3009
BstDEI CTNAG 7 cut(s) 291, 314, 1446, 1683, 2064, 2280, 2894
BstDSI CCRYGG 1 cut(s) 950
BstHHI GCGC 1 cut(s) 2220
BstKTI GATC 7 cut(s) 922, 976, 1248, 1721, 2185, 2779, 3057
BstMAI GTCTC 4 cut(s) 648, 1174, 2067, 3084
BstMBI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
BstMWI GCNNNNNNNGC 6 cut(s) 248, 534, 1352, 1955, 2065, 3209
BstNI CCWGG 3 cut(s) 960, 1647, 2159
BstNSI RCATGY 3 cut(s) 1278, 2110, 2385
BstSCI CCNGG 5 cut(s) 958, 1645, 2157, 3168, 3224
BstSFI CTRYAG 6 cut(s) 162, 366, 570, 1897, 1956, 3342
BstSLI GKGCMC 1 cut(s) 1411
BstV1I GCAGC 8 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2578, 3059
BstV2I GAAGAC 1 cut(s) 3167
BstX2I RGATCY 1 cut(s) 2776
BstYI RGATCY 1 cut(s) 2776
Bsu15I ATCGAT 1 cut(s) 633
BsuI GTATCC 3 cut(s) 2479, 2602, 3285
BsuRI GGCC 5 cut(s) 958, 1196, 1409, 3127, 3173
BsuTUI ATCGAT 1 cut(s) 633
BtgI CCRYGG 1 cut(s) 950
BtsI GCAGTG 6 cut(s) 1845, 2586, 2709, 2850, 3006, 3226
BveI ACCTGC 1 cut(s) 1963
Cac8I GCNNGC 8 cut(s) 667, 2216, 2368, 2508, 2631, 2853, 2928, 3009
CaiI CAGNNNCTG 3 cut(s) 578, 1955, 1964
CfoI GCGC 1 cut(s) 2220
Cfr10I RCCGGY 2 cut(s) 626, 740
Cfr13I GGNCC 7 cut(s) 158, 890, 1407, 1408, 3125, 3171, 3245
ClaI ATCGAT 1 cut(s) 633
CsiI ACCWGGT 1 cut(s) 1645
Csp6I GTAC 6 cut(s) 234, 378, 1050, 2101, 2741, 3237
CspAI ACCGGT 1 cut(s) 740
CviQI GTAC 6 cut(s) 234, 378, 1050, 2101, 2741, 3237
DdeI CTNAG 7 cut(s) 291, 314, 1446, 1683, 2064, 2280, 2894
DpnI GATC 7 cut(s) 921, 975, 1247, 1720, 2184, 2778, 3056
DpnII GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
DraIII CACNNNGTG 1 cut(s) 1307
DriI GACNNNNNGTC 1 cut(s) 3076
Eam1104I CTCTTC 5 cut(s) 102, 462, 489, 1276, 3087
Eam1105I GACNNNNNGTC 1 cut(s) 3076
EarI CTCTTC 5 cut(s) 102, 462, 489, 1276, 3087
Ecl136II GAGCTC 1 cut(s) 2891
Eco130I CCWWGG 1 cut(s) 950
Eco24I GRGCYC 3 cut(s) 1411, 2751, 2893
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 3 cut(s) 158, 890, 3245
Eco53kI GAGCTC 1 cut(s) 2891
Eco57I CTGAAG 3 cut(s) 963, 1986, 2444
Eco88I CYCGRG 1 cut(s) 2639
EcoICRI GAGCTC 1 cut(s) 2891
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 1133
EcoRII CCWGG 3 cut(s) 958, 1645, 2157
EcoT14I CCWWGG 1 cut(s) 950
EcoT22I ATGCAT 1 cut(s) 3340
EcoT38I GRGCYC 3 cut(s) 1411, 2751, 2893
ErhI CCWWGG 1 cut(s) 950
FalI AAGNNNNNCTT 8 cut(s) 1533, 1565, 2499, 2531, 2919, 2951, 3280, 3312
FaqI GGGAC 3 cut(s) 232, 876, 2211
FauI CCCGC 1 cut(s) 3052
FblI GTMKAC 1 cut(s) 1697
FriOI GRGCYC 3 cut(s) 1411, 2751, 2893
FspBI CTAG 3 cut(s) 701, 923, 3360
GlaI GCGC 1 cut(s) 2219
GsaI CCCAGC 3 cut(s) 608, 779, 2714
GsuI CTGGAG 3 cut(s) 165, 1650, 2907
HaeIII GGCC 5 cut(s) 958, 1196, 1409, 3127, 3173
HapII CCGG 4 cut(s) 627, 741, 3169, 3225
HhaI GCGC 1 cut(s) 2220
Hin6I GCGC 1 cut(s) 2218
HinP1I GCGC 1 cut(s) 2218
HindIII AAGCTT 3 cut(s) 912, 3210, 3294
HpaII CCGG 4 cut(s) 627, 741, 3169, 3225
HphI GGTGA 8 cut(s) 95, 149, 755, 1304, 1822, 2760, 3276, 3362
Hpy166II GTNNAC 5 cut(s) 698, 1301, 1698, 2584, 2707
Hpy8I GTNNAC 5 cut(s) 698, 1301, 1698, 2584, 2707
HpyCH4IV ACGT 3 cut(s) 555, 713, 2743
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 534, 1352, 1955, 2065, 3209
HpyF3I CTNAG 7 cut(s) 291, 314, 1446, 1683, 2064, 2280, 2894
HpySE526I ACGT 3 cut(s) 555, 713, 2743
HspAI GCGC 1 cut(s) 2218
Kzo9I GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
Lsp1109I GCAGC 8 cut(s) 260, 1350, 1437, 1961, 2065, 2350, 2578, 3059
MabI ACCWGGT 1 cut(s) 1645
MaeI CTAG 3 cut(s) 701, 923, 3360
MaeII ACGT 3 cut(s) 555, 713, 2743
MaeIII GTNAC 6 cut(s) 199, 317, 1396, 1675, 2394, 2766
MalI GATC 7 cut(s) 921, 975, 1247, 1720, 2184, 2778, 3056
MboI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
MfeI CAATTG 1 cut(s) 2186
MflI RGATCY 1 cut(s) 2776
MhlI GDGCHC 4 cut(s) 1159, 1411, 2751, 2893
MlyI GAGTC 9 cut(s) 214, 647, 1380, 1796, 2294, 2338, 2402, 2774, 3086
MmeI TCCRAC 5 cut(s) 562, 696, 1412, 1864, 2265
Mph1103I ATGCAT 1 cut(s) 3340
MspA1I CMGCKG 4 cut(s) 608, 1450, 2591, 2915
MspI CCGG 4 cut(s) 627, 741, 3169, 3225
MspR9I CCNGG 5 cut(s) 960, 1647, 2159, 3170, 3226
MunI CAATTG 1 cut(s) 2186
Mva1269I GAATGC 3 cut(s) 250, 359, 667
MvaI CCWGG 3 cut(s) 960, 1647, 2159
MwoI GCNNNNNNNGC 6 cut(s) 248, 534, 1352, 1955, 2065, 3209
NciI CCSGG 2 cut(s) 3170, 3226
NcoI CCATGG 1 cut(s) 950
NdeII GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
NlaIV GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
NmuCI GTSAC 4 cut(s) 199, 317, 2394, 2766
NsiI ATGCAT 1 cut(s) 3340
NspI RCATGY 3 cut(s) 1278, 2110, 2385
PaeR7I CTCGAG 1 cut(s) 2639
PaqCI CACCTGC 1 cut(s) 1963
PciI ACATGT 1 cut(s) 2381
PctI GAATGC 3 cut(s) 250, 359, 667
PinAI ACCGGT 1 cut(s) 740
PleI GAGTC 9 cut(s) 214, 646, 1380, 1796, 2294, 2337, 2401, 2773, 3085
PpsI GAGTC 9 cut(s) 214, 646, 1380, 1796, 2294, 2337, 2401, 2773, 3085
Ppu21I YACGTR 1 cut(s) 2744
PpuMI RGGWCCY 1 cut(s) 158
PscI ACATGT 1 cut(s) 2381
PshBI ATTAAT 1 cut(s) 1251
Psp124BI GAGCTC 1 cut(s) 2893
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 3 cut(s) 958, 1645, 2157
PspFI CCCAGC 3 cut(s) 604, 775, 2710
PspGI CCWGG 3 cut(s) 958, 1645, 2157
PspN4I GGNNCC 8 cut(s) 363, 392, 676, 840, 892, 944, 1409, 2163
PspOMI GGGCCC 1 cut(s) 1407
PspPI GGNCC 7 cut(s) 158, 890, 1407, 1408, 3125, 3171, 3245
PspPPI RGGWCCY 1 cut(s) 158
PstI CTGCAG 2 cut(s) 1960, 3346
PstNI CAGNNNCTG 3 cut(s) 578, 1955, 1964
PsuI RGATCY 1 cut(s) 2776
PvuII CAGCTG 3 cut(s) 608, 1450, 2591
RsaI GTAC 6 cut(s) 235, 379, 1051, 2102, 2742, 3238
RsaNI GTAC 6 cut(s) 234, 378, 1050, 2101, 2741, 3237
SacI GAGCTC 1 cut(s) 2893
Sau3AI GATC 7 cut(s) 919, 973, 1245, 1718, 2182, 2776, 3054
Sau96I GGNCC 7 cut(s) 158, 890, 1407, 1408, 3125, 3171, 3245
SchI GAGTC 9 cut(s) 214, 647, 1380, 1796, 2294, 2338, 2402, 2774, 3086
ScrFI CCNGG 5 cut(s) 960, 1647, 2159, 3170, 3226
SduI GDGCHC 4 cut(s) 1159, 1411, 2751, 2893
SexAI ACCWGGT 1 cut(s) 1645
SfcI CTRYAG 6 cut(s) 162, 366, 570, 1897, 1956, 3342
Sfr274I CTCGAG 1 cut(s) 2639
SinI GGWCC 3 cut(s) 158, 890, 3245
SlaI CTCGAG 1 cut(s) 2639
SmlI CTYRAG 6 cut(s) 1171, 1759, 2516, 2639, 2936, 3269
SmoI CTYRAG 6 cut(s) 1171, 1759, 2516, 2639, 2936, 3269
SsiI CCGC 8 cut(s) 593, 1356, 1808, 2837, 2913, 3045, 3190, 3278
SspI AATATT 3 cut(s) 1935, 2205, 3109
SspMI CTAG 3 cut(s) 701, 923, 3360
SstI GAGCTC 1 cut(s) 2893
StyD4I CCNGG 5 cut(s) 958, 1645, 2157, 3168, 3224
StyI CCWWGG 1 cut(s) 950
TaiI ACGT 3 cut(s) 558, 716, 2746
TaqI TCGA 5 cut(s) 278, 398, 633, 1389, 2640
TatI WGTACW 1 cut(s) 1049
TauI GCSGC 2 cut(s) 1358, 3193
TseFI GTSAC 4 cut(s) 199, 317, 2394, 2766
TseI GCWGC 8 cut(s) 248, 1363, 1450, 1949, 2078, 2363, 2591, 3047
Tsp45I GTSAC 4 cut(s) 199, 317, 2394, 2766
VpaK11BI GGWCC 3 cut(s) 158, 890, 3245
VspI ATTAAT 1 cut(s) 1251
XapI RAATTY 4 cut(s) 905, 1133, 2138, 2969
XceI RCATGY 3 cut(s) 1278, 2110, 2385
XhoI CTCGAG 1 cut(s) 2639
XmiI GTMKAC 1 cut(s) 1697
XspI CTAG 3 cut(s) 701, 923, 3360
Zsp2I ATGCAT 1 cut(s) 3340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.