Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0000750

Physical Location & Seq

Genomic Coordinates Reverse (-)

20281571 .. 20287410

Gene Structure 5840 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g26550.t10

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGATTTCTGAGATGCCGGCGACGACGGAGGAGCCGAACACAAAGGCGATGACGGACACGGAGGCGCAAACCTCCATGACGATGATCGACGAGGTCTACGAATTCTCGGCTCCGCGATTCTTCGATTTTATGAAGGAGGAGAGCGACGCCGACAAGAACAAGGCCGAGCTCTGGTTCGACTCCGCTCGCCCCTACGCCCCTTCCCCTTTCATGCCTAGAATCAAGACTGGCAGATCTTTTACAGTGGAGAGCATGTGCGATTTTACTGAAGCAGAACATATGCAGAAGAACCTTGAATCATCCTCTGATTTAAAAGCTGCTGATGAAACTAAGGCTGACTTGAAGATCACTCCTGAATTGAAGGAAGAAACAATTCCCAGGGAGGCTAAAGATGAAAACAGTGCAAGACAAGCCATTACGATTTCTGCTGATAAAGTAGAGGACAAATGCATGAAAGATGATGGAAGTGTTCTGGCTGCTGCAGTATCTTTCAATAAGAGAACTGATATAACTAATGAGAAGGAGAACAGTAATTCCATAGCTAGTACTCCAAAGCCACCCATGAAATGTTCCAAGAAGGGAGCCATTACGATTTCTGCTGATAAAGTAGAGGACAAATGCATGAAAGATGATGGAAGTGTTCTGGCTGCTGCAGTATCTTTCAATAAGAGAACTGATATAACTAATGAGAAGGAGAACAGTAATTCCATAGCTAGTACTCCAAAGCCACCCATGAAATGTTCCAAGAAGGGAGATCTTCAAAGTGATTCCAAAAACCACCAGACAGCTAAAAAGATATCAAGTATGGTCAGAAACCCATCAGGGCTAAGGGGAAAAAGTCACTTGCACTCTTCACAAACTAAACCGTATACTATGAAAAGAGAAGAGAATGTGAAAAAAGCAACTGGCACATCCTTGTTAGCTCAGGAGAATCAGGCAATTAAGCGGCAGAAGTTGGATGCTGGAAGATCTAGACAGATTCTTAATCCCAAACCCCAAACATTGCTCCACAAGTCAAAACTGGGTCATACCAGTGCCACACATTTTTCAACTGCTATTAAAACTCAAAAAGAGGACAGGATTGTGTACGTTCGGGAACCCGCAGCACCATTTGTTTCAATGGCAGAAATGATGAACAAGTTCCAATCAAGTACCAGAGACTTGTCACTGCCGCATGCTAATGGTTCTCTTTCACATATGAAGCCTAAACTCACCTTAACAAGACCAAAGGAGCCAGAATTTGAGACATCACAGCGTGTGCGCTCAGTTAGAGTGAAGAGTACTGCTGAGCTGGAAGAAGAGATGATGGCCAAAATTCCAAAGTTTAAGGCTCGTCCAGTAAACAAAAAGATTCTTGAAGCACCATCATTACCTGCAATGTCAAGAAGTACCCCACAGCCACCTGCATTTCAGGAATTCCATTTAGAGACTATGGCAAGGGCTAACCACAATGCAGAAACAGCCTCAGTAGCTTCAATGGATGTATCACGACGACAGAATAAACAAACAAAGCCCTATCATCTTACTGAACCTAAAACTCCTTTGCTACATACATCATTGAGGGCCCGTCCACCTAGAGTGAAAACTTCTTCGGAAATTGAGCAGGAAGAAATTGAAAAAATCCCAAAATTCAAGGCAAGGCCTTTGAATAAGAAGATATTTGAAAGTAAAGGAGATCTGGGGATGTTCTGCAATGCCAAGAAACATGTGACTGTTCCTCAGGAATTTCACTTTGCAACAAATGAAAGAATACCACCACCACCACCACCACCACCTTCAGCCATTCATGATCTATTTGATAAGTTGTCTTTGAACTCGGAGCCCCAACAGAATCCAATTCCAAGAAACACAATGCCAAATCCATTTCATCTCCATACAGAGGAAAGAGGGGCTGAGAAGGAAAAGAAATTTATTACCGAAGTTATGCAGAAGCAAATGGAAGAAGAAAGAGCTAGAGTTCCCAAAGCGAATCCATATCCATATACTACTGACTACCCAGTGATCCCACCAAAGCCAGAGCCCAAGGAATGTACAAAGCCGGAACCTTTCCTGCTAGAAAGCTTAGTGAGGCATGAGGAAGAAATACAAAGGGAAATGGAAGAAAGACGGAGGATGGAGATGGAAGAAGCCCAGTCAAGGGTTTTCAAGGCACAACCAATCTTAAAAGAGGACCCAATTCCAGTTCCAGAGAAAGTCCGTAAGCCTCTAACACAAGTTCAGGAGTTCAATCTGAATGTTGATAATCGGGCAGTGCAAAGAGCAGAATTTGATTACAAGGTTAAGGAGAAAGAGATGATATATAAGAGATATAGAGAAGAAAGTGAAGCAGCAAGGATGTTAGAGGAAGAGAAATCATTGAAACATATGAGAAGGACGATGGTACCTCATGCAAGACCGGTGCCTAATTTTGAGCATCCATTTTGCCCCCAAAAGTGTCCAAGGGAAACAACAAAAGCAAAGTCCCCGAATCTGCGAGTCCTACAGCGGCAAGAAAGGCGAAGGATGATGCTCAAATCCGCCACTTCCAGCGCTGCTGCACACATGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032886 GO:0033043 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050789 GO:0050794 GO:0051128 GO:0051225 GO:0051493 GO:0051726 GO:0060236 GO:0065007 GO:0070013 GO:0070507 GO:0070925 GO:0071840 GO:0090224 GO:0090307 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2 TPX2_importin

Protein Analysis

850

Amino Acids

96.86

Weight (kDa)

9.31

Isoelectric Point (pI)

55.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2_importin	PF12214	348 - 443	2.2e-30	Cell cycle regulated microtubule associated protein
TPX2_importin	PF12214	450 - 527	1.1e-18	Cell cycle regulated microtubule associated protein
TPX2	PF06886	685 - 760	4.8e-21	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03780 AT1G03780 AT1G03780
fragaria_vesca	FvH4_2g11956 FvH4_5g28370 FvH4_5g28401 FvH4_5g28402 FvH4_5g28440 FvH4_5g28450 FvH4_5g28450 FvH4_5g28471 FvH4_5g28480 FvH4_5g28561 FvH4_6g25610 FvH4_6g25610 FvH4_6g25690 FvH4_6g25731 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_c15g00040 FvH4_c17g00020 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090
malus_domestica	MD09G1243900.v1.1 MD17G1235800.v1.1
prunus_persica	Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1
pyrus_communis	pycom17g23880
rosa_chinensis	RchiOBHm_Chr2g0127601
rosa_laevigata	RLG00000018552 RLG00000018951
rosa_multiflora	Rmu_ssc0000200.1_g000020
rosa_roxburghii	Rroxscaffold_2G00116560 Rroxscaffold_2G00123480
rosa_rugosa	Rorug02G0272600
rosa_samantha	Rh2AG324600 Rh2BG333900 Rh2CG312100 Rh2DG352100
rosa_wichuraiana	Rw2G026360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1411
Acc36I	ACCTGC	2 cut(s)	1381, 1411
Acc65I	GGTACC	1 cut(s)	2380
AccB1I	GGYRCC	2 cut(s)	2380, 2398
AccBSI	CCGCTC	1 cut(s)	185
AccI	GTMKAC	2 cut(s)	96, 869
AccII	CGCG	1 cut(s)	115
AciI	CCGC	7 cut(s)	113, 183, 946, 1101, 1172, 2485, 2517
AclWI	GGATC	1 cut(s)	1996
AcoI	YGGCCR	1 cut(s)	1308
AcsI	RAATTY	8 cut(s)	101, 1238, 1314, 1415, 1628, 1724, 1907, 2264
AcuI	CTGAAG	2 cut(s)	288, 1761
AcyI	GRCGYC	1 cut(s)	147
AdeI	CACNNNGTG	1 cut(s)	1256
AfaI	GTAC	8 cut(s)	547, 718, 1088, 1153, 1282, 1390, 2032, 2382
AfeI	AGCGCT	1 cut(s)	2530
AfiI	CCNNNNNNNGG	4 cut(s)	171, 1879, 2136, 2137
AflIII	ACRYGT	1 cut(s)	1705
AgeI	ACCGGT	1 cut(s)	2395
AjnI	CCWGG	1 cut(s)	377
AloI	GAACNNNNNNTCC	4 cut(s)	518, 550, 689, 721
Alw21I	GWGCWC	1 cut(s)	171
Alw26I	GTCTC	3 cut(s)	1152, 1238, 1421
AlwI	GGATC	1 cut(s)	1996
Aor51HI	AGCGCT	1 cut(s)	2530
AoxI	GGCC	4 cut(s)	162, 1308, 1563, 1640
ApaI	GGGCCC	1 cut(s)	1567
ApeKI	GCWGC	9 cut(s)	317, 476, 479, 647, 650, 1103, 2327, 2531, 2534
ApoI	RAATTY	8 cut(s)	101, 1238, 1314, 1415, 1628, 1724, 1907, 2264
ArsI	GACNNNNNNTTYG	2 cut(s)	2444, 2476
AsiGI	ACCGGT	1 cut(s)	2395
Asp700I	GAANNNNTTC	2 cut(s)	372, 1139
Asp718I	GGTACC	1 cut(s)	2380
AspLEI	GCGC	3 cut(s)	67, 1263, 2531
AspS9I	GGNCC	3 cut(s)	1563, 1564, 2170
AsuHPI	GGTGA	1 cut(s)	1204
AvaII	GGWCC	1 cut(s)	2170
AxyI	CCTNAGG	1 cut(s)	1719
BaeGI	GKGCMC	1 cut(s)	1567
BaeI	ACNNNNGTAYC	2 cut(s)	2364, 2397
BalI	TGGCCA	1 cut(s)	1310
BanI	GGYRCC	2 cut(s)	2380, 2398
BanII	GRGCYC	4 cut(s)	171, 1567, 1824, 2022
Bbv12I	GWGCWC	1 cut(s)	171
BbvI	GCAGC	9 cut(s)	304, 463, 466, 634, 637, 1115, 2339, 2518, 2521
BccI	CCATC	8 cut(s)	455, 626, 826, 1300, 1372, 2107, 2113, 2371
BciT130I	CCWGG	1 cut(s)	379
BcoDI	GTCTC	3 cut(s)	1152, 1238, 1421
BfaI	CTAG	7 cut(s)	216, 543, 714, 972, 1575, 1953, 2054
BfmI	CTRYAG	3 cut(s)	480, 651, 2480
BfoI	RGCGCY	1 cut(s)	2532
BfuAI	ACCTGC	2 cut(s)	1381, 1411
BglII	AGATCT	4 cut(s)	233, 754, 968, 1675
BlpI	GCTNAGC	1 cut(s)	1287
BmcAI	AGTACT	3 cut(s)	547, 718, 1282
Bme1390I	CCNGG	1 cut(s)	379
Bme18I	GGWCC	1 cut(s)	2170
BmgT120I	GGNCC	3 cut(s)	1563, 1564, 2170
BmrFI	CCNGG	1 cut(s)	379
BmrI	ACTGGG	3 cut(s)	1031, 1991, 2125
BmsI	GCATC	4 cut(s)	3, 949, 2422, 2496
BmuI	ACTGGG	3 cut(s)	1031, 1991, 2125
Bpu10I	CCTNAGC	2 cut(s)	827, 924
Bpu1102I	GCTNAGC	1 cut(s)	1287
BsaHI	GRCGYC	1 cut(s)	147
BsaJI	CCNNGG	4 cut(s)	377, 378, 2022, 2438
BsaWI	WCCGGW	1 cut(s)	2395
BsaXI	ACNNNNNCTCC	2 cut(s)	239, 269
Bsc4I	CCNNNNNNNGG	4 cut(s)	171, 1879, 2136, 2137
Bse118I	RCCGGY	2 cut(s)	16, 2395
Bse1I	ACTGG	8 cut(s)	232, 910, 1026, 1032, 1337, 1997, 2131, 2180
Bse21I	CCTNAGG	1 cut(s)	1719
Bse3DI	GCAATG	3 cut(s)	1001, 1383, 1699
BseBI	CCWGG	1 cut(s)	379
BseDI	CCNNGG	4 cut(s)	377, 378, 2022, 2438
BseGI	GGATG	9 cut(s)	299, 911, 964, 1486, 1689, 2118, 2340, 2413, 2508
BseLI	CCNNNNNNNGG	4 cut(s)	171, 1879, 2136, 2137
BseMI	GCAATG	3 cut(s)	1001, 1383, 1699
BseMII	CTCAG	6 cut(s)	938, 1278, 1278, 1479, 1733, 1884
BseNI	ACTGG	8 cut(s)	232, 910, 1026, 1032, 1337, 1997, 2131, 2180
BseRI	GAGGAG	2 cut(s)	44, 152
BseSI	GKGCMC	1 cut(s)	1567
BseXI	GCAGC	9 cut(s)	304, 463, 466, 634, 637, 1115, 2339, 2518, 2521
BsgI	GTGCAG	1 cut(s)	2520
Bsh1236I	CGCG	1 cut(s)	115
BshFI	GGCC	4 cut(s)	164, 1310, 1565, 1642
BshNI	GGYRCC	2 cut(s)	2380, 2398
BshTI	ACCGGT	1 cut(s)	2395
BsiHKAI	GWGCWC	1 cut(s)	171
BsiSI	CCGG	3 cut(s)	17, 2039, 2396
BslFI	GGGAC	1 cut(s)	2446
BslI	CCNNNNNNNGG	4 cut(s)	171, 1879, 2136, 2137
BsmAI	GTCTC	3 cut(s)	1152, 1238, 1421
BsmFI	GGGAC	1 cut(s)	2446
BsnI	GGCC	4 cut(s)	164, 1310, 1565, 1642
Bsp120I	GGGCCC	1 cut(s)	1563
Bsp1286I	GDGCHC	4 cut(s)	171, 1567, 1824, 2022
Bsp1407I	TGTACA	1 cut(s)	2030
Bsp143I	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
Bsp1720I	GCTNAGC	1 cut(s)	1287
BspACI	CCGC	7 cut(s)	113, 183, 946, 1101, 1172, 2485, 2517
BspANI	GGCC	4 cut(s)	164, 1310, 1565, 1642
BspCNI	CTCAG	6 cut(s)	937, 1277, 1279, 1478, 1732, 1885
BspFNI	CGCG	1 cut(s)	115
BspHI	TCATGA	1 cut(s)	1786
BspMAI	CTGCAG	2 cut(s)	484, 655
BspMI	ACCTGC	2 cut(s)	1381, 1411
BspPI	GGATC	1 cut(s)	1996
BspT107I	GGYRCC	2 cut(s)	2380, 2398
BsrBI	CCGCTC	1 cut(s)	185
BsrDI	GCAATG	3 cut(s)	1001, 1383, 1699
BsrFI	RCCGGY	2 cut(s)	16, 2395
BsrGI	TGTACA	1 cut(s)	2030
BsrI	ACTGG	8 cut(s)	232, 910, 1026, 1032, 1337, 1997, 2131, 2180
BssAI	RCCGGY	2 cut(s)	16, 2395
BssECI	CCNNGG	4 cut(s)	377, 378, 2022, 2438
BssMI	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
BssNAI	GTATAC	1 cut(s)	870
BssNI	GRCGYC	1 cut(s)	147
BssT1I	CCWWGG	2 cut(s)	2022, 2438
Bst1107I	GTATAC	1 cut(s)	870
Bst2UI	CCWGG	1 cut(s)	379
Bst4CI	ACNGT	6 cut(s)	244, 401, 530, 701, 867, 1714
Bst6I	CTCTTC	5 cut(s)	856, 879, 1271, 1293, 2340
BstACI	GRCGYC	1 cut(s)	147
BstAUI	TGTACA	1 cut(s)	2030
BstC8I	GCNNGC	3 cut(s)	18, 187, 1176
BstF5I	GGATG	9 cut(s)	299, 911, 964, 1486, 1689, 2118, 2340, 2413, 2508
BstFNI	CGCG	1 cut(s)	115
BstH2I	RGCGCY	1 cut(s)	2532
BstHHI	GCGC	3 cut(s)	67, 1263, 2531
BstKTI	GATC	8 cut(s)	87, 236, 348, 757, 971, 1678, 1792, 2004
BstMAI	GTCTC	3 cut(s)	1152, 1238, 1421
BstMBI	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
BstMWI	GCNNNNNNNGC	4 cut(s)	410, 1460, 1469, 2494
BstNI	CCWGG	1 cut(s)	379
BstNSI	RCATGY	3 cut(s)	256, 1178, 1709
BstSCI	CCNGG	1 cut(s)	377
BstSFI	CTRYAG	3 cut(s)	480, 651, 2480
BstSLI	GKGCMC	1 cut(s)	1567
BstUI	CGCG	1 cut(s)	115
BstV1I	GCAGC	9 cut(s)	304, 463, 466, 634, 637, 1115, 2339, 2518, 2521
BstX2I	RGATCY	4 cut(s)	233, 754, 968, 1675
BstYI	RGATCY	4 cut(s)	233, 754, 968, 1675
BstZ17I	GTATAC	1 cut(s)	870
Bsu36I	CCTNAGG	1 cut(s)	1719
BsuRI	GGCC	4 cut(s)	164, 1310, 1565, 1642
BtgZI	GCGATG	1 cut(s)	62
BtsCI	GGATG	9 cut(s)	299, 911, 964, 1486, 1689, 2118, 2340, 2413, 2508
BtsI	GCAGTG	2 cut(s)	1166, 2256
BtsIMutI	CAGTG	6 cut(s)	249, 406, 1039, 1166, 2004, 2256
BveI	ACCTGC	2 cut(s)	1381, 1411
Cac8I	GCNNGC	3 cut(s)	18, 187, 1176
CciI	TCATGA	1 cut(s)	1786
CfoI	GCGC	3 cut(s)	67, 1263, 2531
Cfr10I	RCCGGY	2 cut(s)	16, 2395
Cfr13I	GGNCC	3 cut(s)	1563, 1564, 2170
CseI	GACGC	1 cut(s)	155
Csp6I	GTAC	8 cut(s)	546, 717, 1087, 1152, 1281, 1389, 2031, 2381
CspAI	ACCGGT	1 cut(s)	2395
CviQI	GTAC	8 cut(s)	546, 717, 1087, 1152, 1281, 1389, 2031, 2381
DpnI	GATC	8 cut(s)	86, 235, 347, 756, 970, 1677, 1791, 2003
DpnII	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
DraI	TTTAAA	1 cut(s)	312
DraIII	CACNNNGTG	1 cut(s)	1256
EaeI	YGGCCR	1 cut(s)	1308
Eam1104I	CTCTTC	5 cut(s)	856, 879, 1271, 1293, 2340
EarI	CTCTTC	5 cut(s)	856, 879, 1271, 1293, 2340
EciI	GGCGGA	1 cut(s)	2506
Ecl136II	GAGCTC	1 cut(s)	169
Eco130I	CCWWGG	2 cut(s)	2022, 2438
Eco147I	AGGCCT	1 cut(s)	1642
Eco24I	GRGCYC	4 cut(s)	171, 1567, 1824, 2022
Eco32I	GATATC	1 cut(s)	798
Eco47I	GGWCC	1 cut(s)	2170
Eco47III	AGCGCT	1 cut(s)	2530
Eco53kI	GAGCTC	1 cut(s)	169
Eco57I	CTGAAG	2 cut(s)	288, 1761
Eco81I	CCTNAGG	1 cut(s)	1719
EcoICRI	GAGCTC	1 cut(s)	169
EcoO109I	RGGNCCY	2 cut(s)	1563, 2170
EcoRI	GAATTC	2 cut(s)	101, 1415
EcoRII	CCWGG	1 cut(s)	377
EcoRV	GATATC	1 cut(s)	798
EcoT14I	CCWWGG	2 cut(s)	2022, 2438
EcoT22I	ATGCAT	2 cut(s)	452, 623
EcoT38I	GRGCYC	4 cut(s)	171, 1567, 1824, 2022
ErhI	CCWWGG	2 cut(s)	2022, 2438
FalI	AAGNNNNNCTT	2 cut(s)	323, 355
FaqI	GGGAC	1 cut(s)	2446
FauI	CCCGC	1 cut(s)	1108
FauNDI	CATATG	3 cut(s)	279, 1197, 2364
FblI	GTMKAC	2 cut(s)	96, 869
FokI	GGATG	9 cut(s)	286, 898, 971, 1493, 1696, 2125, 2347, 2400, 2515
FriOI	GRGCYC	4 cut(s)	171, 1567, 1824, 2022
FspBI	CTAG	7 cut(s)	216, 543, 714, 972, 1575, 1953, 2054
GlaI	GCGC	3 cut(s)	66, 1262, 2530
HaeII	RGCGCY	1 cut(s)	2532
HaeIII	GGCC	4 cut(s)	164, 1310, 1565, 1642
HapII	CCGG	3 cut(s)	17, 2039, 2396
HgaI	GACGC	1 cut(s)	155
HhaI	GCGC	3 cut(s)	67, 1263, 2531
Hin1I	GRCGYC	1 cut(s)	147
Hin6I	GCGC	3 cut(s)	65, 1261, 2529
HinP1I	GCGC	3 cut(s)	65, 1261, 2529
HindIII	AAGCTT	1 cut(s)	2059
HpaII	CCGG	3 cut(s)	17, 2039, 2396
HphI	GGTGA	1 cut(s)	1204
Hpy166II	GTNNAC	5 cut(s)	97, 870, 1087, 1342, 1571
Hpy188I	TCNGA	6 cut(s)	10, 307, 812, 1594, 1819, 2232
Hpy8I	GTNNAC	5 cut(s)	97, 870, 1087, 1342, 1571
Hpy99I	CGWCG	5 cut(s)	25, 28, 92, 149, 1494
HpyCH4III	ACNGT	6 cut(s)	244, 401, 530, 701, 867, 1714
HpyCH4IV	ACGT	1 cut(s)	1089
HpyF10VI	GCNNNNNNNGC	4 cut(s)	410, 1460, 1469, 2494
HpySE526I	ACGT	1 cut(s)	1089
Hsp92I	GRCGYC	1 cut(s)	147
HspAI	GCGC	3 cut(s)	65, 1261, 2529
KpnI	GGTACC	1 cut(s)	2384
KroI	GCCGGC	1 cut(s)	16
KroNI	GCCGGC	1 cut(s)	18
Kzo9I	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
LmnI	GCTCC	6 cut(s)	31, 115, 581, 1011, 1231, 1819
Lsp1109I	GCAGC	9 cut(s)	304, 463, 466, 634, 637, 1115, 2339, 2518, 2521
LweI	GCATC	4 cut(s)	3, 949, 2422, 2496
MaeI	CTAG	7 cut(s)	216, 543, 714, 972, 1575, 1953, 2054
MaeII	ACGT	1 cut(s)	1089
MaeIII	GTNAC	3 cut(s)	839, 1164, 1708
MalI	GATC	8 cut(s)	86, 235, 347, 756, 970, 1677, 1791, 2003
MbiI	CCGCTC	1 cut(s)	185
MboI	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
MflI	RGATCY	4 cut(s)	233, 754, 968, 1675
MhlI	GDGCHC	4 cut(s)	171, 1567, 1824, 2022
MlsI	TGGCCA	1 cut(s)	1310
MluNI	TGGCCA	1 cut(s)	1310
MlyI	GAGTC	2 cut(s)	173, 2484
MmeI	TCCRAC	1 cut(s)	936
Mox20I	TGGCCA	1 cut(s)	1310
Mph1103I	ATGCAT	2 cut(s)	452, 623
MroNI	GCCGGC	1 cut(s)	16
MroXI	GAANNNNTTC	2 cut(s)	372, 1139
MscI	TGGCCA	1 cut(s)	1310
MseI	TTAA	8 cut(s)	311, 942, 984, 1059, 1217, 1326, 2162, 2280
MslI	CAYNNNNRTG	3 cut(s)	80, 1032, 1179
Msp20I	TGGCCA	1 cut(s)	1310
MspA1I	CMGCKG	1 cut(s)	2485
MspI	CCGG	3 cut(s)	17, 2039, 2396
MspR9I	CCNGG	1 cut(s)	379
MvaI	CCWGG	1 cut(s)	379
MvnI	CGCG	1 cut(s)	115
MwoI	GCNNNNNNNGC	4 cut(s)	410, 1460, 1469, 2494
NaeI	GCCGGC	1 cut(s)	18
NdeI	CATATG	3 cut(s)	279, 1197, 2364
NdeII	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
NgoMIV	GCCGGC	1 cut(s)	16
NmeAIII	GCCGAG	2 cut(s)	86, 190
NmuCI	GTSAC	3 cut(s)	839, 1164, 1708
NsiI	ATGCAT	2 cut(s)	452, 623
NspI	RCATGY	3 cut(s)	256, 1178, 1709
PaeI	GCATGC	1 cut(s)	1178
PagI	TCATGA	1 cut(s)	1786
PaqCI	CACCTGC	1 cut(s)	1411
PasI	CCCWGGG	1 cut(s)	378
PceI	AGGCCT	1 cut(s)	1642
PciI	ACATGT	1 cut(s)	1705
PcsI	WCGNNNNNNNCGW	2 cut(s)	32, 96
PdiI	GCCGGC	1 cut(s)	18
PdmI	GAANNNNTTC	2 cut(s)	372, 1139
PflFI	GACNNNGTC	1 cut(s)	92
PinAI	ACCGGT	1 cut(s)	2395
PleI	GAGTC	2 cut(s)	173, 2483
PpsI	GAGTC	2 cut(s)	173, 2483
PpuMI	RGGWCCY	1 cut(s)	2170
PscI	ACATGT	1 cut(s)	1705
Psp124BI	GAGCTC	1 cut(s)	171
Psp5II	RGGWCCY	1 cut(s)	2170
Psp6I	CCWGG	1 cut(s)	377
PspGI	CCWGG	1 cut(s)	377
PspOMI	GGGCCC	1 cut(s)	1563
PspPI	GGNCC	3 cut(s)	1563, 1564, 2170
PspPPI	RGGWCCY	1 cut(s)	2170
PstI	CTGCAG	2 cut(s)	484, 655
PsuI	RGATCY	4 cut(s)	233, 754, 968, 1675
PsyI	GACNNNGTC	1 cut(s)	92
RsaI	GTAC	8 cut(s)	547, 718, 1088, 1153, 1282, 1390, 2032, 2382
RsaNI	GTAC	8 cut(s)	546, 717, 1087, 1152, 1281, 1389, 2031, 2381
RseI	CAYNNNNRTG	3 cut(s)	80, 1032, 1179
SacI	GAGCTC	1 cut(s)	171
SaqAI	TTAA	8 cut(s)	311, 942, 984, 1059, 1217, 1326, 2162, 2280
Sau3AI	GATC	8 cut(s)	84, 233, 345, 754, 968, 1675, 1789, 2001
Sau96I	GGNCC	3 cut(s)	1563, 1564, 2170
ScaI	AGTACT	3 cut(s)	547, 718, 1282
SchI	GAGTC	2 cut(s)	173, 2484
ScrFI	CCNGG	1 cut(s)	379
SduI	GDGCHC	4 cut(s)	171, 1567, 1824, 2022
SfaNI	GCATC	4 cut(s)	3, 949, 2422, 2496
SfcI	CTRYAG	3 cut(s)	480, 651, 2480
SinI	GGWCC	1 cut(s)	2170
SmiMI	CAYNNNNRTG	3 cut(s)	80, 1032, 1179
SphI	GCATGC	1 cut(s)	1178
SseBI	AGGCCT	1 cut(s)	1642
SsiI	CCGC	7 cut(s)	113, 183, 946, 1101, 1172, 2485, 2517
SspMI	CTAG	7 cut(s)	216, 543, 714, 972, 1575, 1953, 2054
SstI	GAGCTC	1 cut(s)	171
StuI	AGGCCT	1 cut(s)	1642
StyD4I	CCNGG	1 cut(s)	377
StyI	CCWWGG	2 cut(s)	2022, 2438
TaaI	ACNGT	6 cut(s)	244, 401, 530, 701, 867, 1714
TaiI	ACGT	1 cut(s)	1092
TaqI	TCGA	3 cut(s)	87, 123, 177
TatI	WGTACW	4 cut(s)	545, 716, 1280, 2030
TauI	GCSGC	3 cut(s)	949, 1174, 2488
Tru1I	TTAA	8 cut(s)	311, 942, 984, 1059, 1217, 1326, 2162, 2280
Tru9I	TTAA	8 cut(s)	311, 942, 984, 1059, 1217, 1326, 2162, 2280
TscAI	CASTG	6 cut(s)	249, 406, 1039, 1173, 2004, 2256
TseFI	GTSAC	3 cut(s)	839, 1164, 1708
TseI	GCWGC	9 cut(s)	317, 476, 479, 647, 650, 1103, 2327, 2531, 2534
Tsp45I	GTSAC	3 cut(s)	839, 1164, 1708
TspGWI	ACGGA	5 cut(s)	41, 68, 74, 2122, 2186
TspRI	CASTG	6 cut(s)	249, 406, 1039, 1173, 2004, 2256
Tth111I	GACNNNGTC	1 cut(s)	92
VpaK11BI	GGWCC	1 cut(s)	2170
XapI	RAATTY	8 cut(s)	101, 1238, 1314, 1415, 1628, 1724, 1907, 2264
XbaI	TCTAGA	1 cut(s)	971
XceI	RCATGY	3 cut(s)	256, 1178, 1709
XmiI	GTMKAC	2 cut(s)	96, 869
XmnI	GAANNNNTTC	2 cut(s)	372, 1139
XspI	CTAG	7 cut(s)	216, 543, 714, 972, 1575, 1953, 2054
ZrmI	AGTACT	3 cut(s)	547, 718, 1282
Zsp2I	ATGCAT	2 cut(s)	452, 623

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.