Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0000750

Physical Location & Seq

Genomic Coordinates Forward (+)

28470661 .. 28475516

Gene Structure 4856 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1235800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGACCGATCAAGAACCCAACGCGCCGGCGGCGACGGCGATCATCGTCGATGAGACGTACGAGTTCTCGGCTCCACGCTTCTTCGACTTCACAAAGGACGAGAGCGACGACGATAGGCTCAAGGCCGAGCTCTGGTTCGACTGCGCTCTAGCCTATGCTCCTTCACCCTTTATGCCTAAGATCAAGACTGGTAGATCCCTTACAATCGAGAGCATGTGTGACTTTAGTGAAGCAGAGAAAATGGAGAAGAACTTGGAATCATCATCTGACTTAAAAGCTGATGATAAGTTGTCTATGGAAACTAAGCCTCAATCAGAGATCATGCCTGCTGAAGCCTTAAAAGATGATGACAAATGTACCAGCGGAAATGGAAGTGTTTTGGATACAAAATCTTCTATTAAGAAAACTGATTTAACCAACGGTAAGGAGGCTAGCAAACCTATCATTCAAGTTGAAGCTGTAGCTTGCACTCCAAGACATGATGACAAATGTACTAGTGGAAACGGAAGTGTTTTGGGTGCAGTGTCTTCTATTAAGAAAACTGATCTGACCAAGGGTAAGGAGAATAGTAAACCTATCATTCAAGATGAAAATGAAGCTTGCACTCCAAAACCACCCATGAGTTATTCCAAGAAGGGGGGGGGAGGCCTTGCGAGTCATTCCAAGAAGCACCAAACAGCTAAAAAGATAGCGAGCATGGTTAGAAACCCCTCAGCACTGAGGCAAAAAAGTCACTTACAGTCATCCCAAACTAAAAGCATTAAACCAAATAGCGTGAAAAGAGAAACACATGTGAAGAATGCAACTGGAACTCCCAATTTAGGACAGGAAAATCAGGCGATAAAGCGGCAGAAATTGGACGAAGGAAGATCTAGACAGATTCTAAATCCCAAACCCCAAGCATATCCCCACAAGTCAAAACTGGGTCATGTAACTTCTTCAGCTGCTGTGCAAAAAGAGGACAGAAAACTTTATGTTAGGGAACCTGCAGCACCATTTGTTTCGACGGCCGAAATGATGAAAAGGTTCCAATCAAGTACCAGAGACTTGTCATTGCCGCATATTTCACATATGAAACCCAAACTCACCTTGACGAGGCCTAAGGAGCCTGAATTTGAAACATCCCAGCGTGTTCGTTCAGTGAGAGTAAAGAGTACTGCTGAGCTTGAAGAAGAGATGATGGCTAAAATTCCCAAGTTTAAGGCTCGACCATTAAACAGAAAGATTCTAGAAGCCTCATCATTGCCTGCAATACCAAGAAGTACACCCCAGCCACCGGAATTTCACGAATTCCATTTGGAGACAATGGCAAGGGCCAATCAGAGTGCAGAAACATCCTCAATAGCTTCAACCGAAGTGTCCCATCAGAATAATCCATGGAAGCCTCGTTTAGGGTTTTTTCTTACGGAACCTAAAACCCCTGTGCTACAAACATCATTGAGGGCTCGTCCACCCAGGGTGAAAAGTTCGTTTGAAATTGAACAGGAAGAACTCGAAAGAATCCCAAAATTCAAGGCCAAGCCTCTGAATAAGAAGATATTTGAAAGTAAAGGAGATATGGGGGTGTTCTGTAATGCCAAGAAACATGTGACCAAACCTCGGGAATTCCATTTTGCAACAAATGAAAGGATACCACCACAATCTTCGTCTATTGTTGATCTATTTGATAAACTGTCGTTAAACTCAGAGCCTCACCAAAAACCAGTTCCAAGAAACACAACGCCAAACCCATTCCATCTCCACACAGAGGAAAGAGGGGCCGAGAAAGAAAGGAAATTCATTACTGAAGTCTGGCACAAGCAATTGGAAGAAGAAAGAGCGAGGGTTCCCAAAGCTAACCCATATCCTTATACCACTGATTACCCTGTGATCCCACCCAAGCCAGAGCCCAAGCAATGTACAAAACCGGAACCTTTCCAGCTGGAGAGTTTAGTGAGGCATGAGGAGGAATTGCAAAGGGAAATGGAAGAAAGACAGCGGAAGGAGAAAGAAGAAGCTCAGATGAGAGTGTTCAAGGCACAGCCAATCTTGAAAGAGGACCCAATTCCAGTTCCCGAAAAAGAACGCAAGCCTCTCACCCAAGTTCAAGAGTTCAATCTCCATGCTGATCATCGAGCTGTTGAAAGAGCAGAATTTGATCACAAAGTTAGGGAGAAAGAGATGATGTATAAGAGATATAGAGAAGAGAGCGAGGCAGCAAGAATGGTGGAAGAAGAGAAGGCACTGAAACAACTCAGAAGGACATTGGTACCCCATGCAAGGCCAGTGCCCAATTTTGACCATCCTTTCCTTCCCCCAAAGTGTCCTAAGGAAGCAACAAAACCGAAATCACCATATCTACGTGTGCTCCAACGGAAAGAAAGGCGAAAAATGGTGATCAACTCTGCAGTTTCCAGCGCAGCGGCACAGATGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032886 GO:0033043 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050789 GO:0050794 GO:0051128 GO:0051225 GO:0051493 GO:0051726 GO:0060236 GO:0065007 GO:0070013 GO:0070507 GO:0070925 GO:0071840 GO:0090224 GO:0090307 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2 TPX2_importin

Protein Analysis

806

Amino Acids

91.7

Weight (kDa)

9.39

Isoelectric Point (pI)

53.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2_importin	PF12214	363 - 456	1.1e-30	Cell cycle regulated microtubule associated protein
TPX2_importin	PF12214	468 - 548	1.8e-18	Cell cycle regulated microtubule associated protein
TPX2	PF06886	698 - 773	9.9e-24	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03780 AT1G03780 AT1G03780
fragaria_vesca	FvH4_2g11956 FvH4_5g28370 FvH4_5g28401 FvH4_5g28402 FvH4_5g28440 FvH4_5g28450 FvH4_5g28450 FvH4_5g28471 FvH4_5g28480 FvH4_5g28561 FvH4_6g25610 FvH4_6g25610 FvH4_6g25690 FvH4_6g25731 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_c15g00040 FvH4_c17g00020 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090
malus_domestica	MD09G1243900.v1.1 MD17G1235800.v1.1
prunus_persica	Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1
pyrus_communis	pycom17g23880
rosa_chinensis	RchiOBHm_Chr2g0127601
rosa_laevigata	RLG00000018552 RLG00000018951
rosa_multiflora	Rmu_ssc0000200.1_g000020
rosa_roxburghii	Rroxscaffold_2G00116560 Rroxscaffold_2G00123480
rosa_rugosa	Rorug02G0272600
rosa_samantha	Rh2AG324600 Rh2BG333900 Rh2CG312100 Rh2DG352100
rosa_wichuraiana	Rw2G026360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	994
Acc65I	GGTACC	1 cut(s)	2248
AccB1I	GGYRCC	1 cut(s)	2248
AccII	CGCG	1 cut(s)	23
AciI	CCGC	6 cut(s)	29, 363, 847, 1058, 1978, 2402
AclWI	GGATC	2 cut(s)	189, 1864
AcoI	YGGCCR	1 cut(s)	1008
AcsI	RAATTY	8 cut(s)	1112, 1188, 1280, 1289, 1508, 1604, 1775, 2132
AcuI	CTGAAG	3 cut(s)	351, 924, 1806
AfaI	GTAC	8 cut(s)	59, 358, 493, 1039, 1156, 1264, 1900, 2250
AfiI	CCNNNNNNNGG	9 cut(s)	132, 636, 821, 1095, 1276, 1391, 1599, 1747, 2259
AflIII	ACRYGT	3 cut(s)	790, 1585, 2341
AhlI	ACTAGT	1 cut(s)	494
AjnI	CCWGG	1 cut(s)	1454
AjuI	GAANNNNNNNTTGG	2 cut(s)	1689, 1721
Alw21I	GWGCWC	2 cut(s)	132, 2349
Alw26I	GTCTC	3 cut(s)	47, 1038, 1295
AlwI	GGATC	2 cut(s)	189, 1864
AlwNI	CAGNNNCTG	1 cut(s)	947
Ama87I	CYCGRG	1 cut(s)	1599
AoxI	GGCC	8 cut(s)	123, 646, 1008, 1097, 1314, 1515, 1758, 2261
ApeKI	GCWGC	4 cut(s)	944, 989, 2195, 2399
ApoI	RAATTY	8 cut(s)	1112, 1188, 1280, 1289, 1508, 1604, 1775, 2132
Asp700I	GAANNNNTTC	1 cut(s)	1025
Asp718I	GGTACC	1 cut(s)	2248
AspLEI	GCGC	3 cut(s)	25, 146, 2399
AspS9I	GGNCC	3 cut(s)	1314, 1758, 2038
AsuHPI	GGTGA	7 cut(s)	156, 1078, 1471, 1685, 2068, 2322, 2386
AsuNHI	GCTAGC	1 cut(s)	431
AvaI	CYCGRG	1 cut(s)	1599
AvaII	GGWCC	1 cut(s)	2038
AxyI	CCTNAGG	2 cut(s)	1101, 2307
BaeGI	GKGCMC	1 cut(s)	2271
BaeI	ACNNNNGTAYC	4 cut(s)	340, 373, 2232, 2265
BanI	GGYRCC	1 cut(s)	2248
BanII	GRGCYC	3 cut(s)	132, 1447, 1890
BbsI	GAAGAC	1 cut(s)	519
Bbv12I	GWGCWC	2 cut(s)	132, 2349
BbvCI	CCTCAGC	1 cut(s)	712
BbvI	GCAGC	4 cut(s)	931, 1001, 2207, 2411
BccI	CCATC	4 cut(s)	1174, 1371, 1743, 2289
BceAI	ACGGC	2 cut(s)	51, 1023
BciT130I	CCWGG	1 cut(s)	1456
BciVI	GTATCC	2 cut(s)	376, 1623
BclI	TGATCA	3 cut(s)	2107, 2137, 2376
BcoDI	GTCTC	3 cut(s)	47, 1038, 1295
BcuI	ACTAGT	1 cut(s)	494
BfaI	CTAG	5 cut(s)	149, 432, 495, 873, 1229
BfmI	CTRYAG	3 cut(s)	459, 987, 2385
BfuAI	ACCTGC	1 cut(s)	994
BfuI	GTATCC	2 cut(s)	376, 1623
BglII	AGATCT	1 cut(s)	869
BisI	GCNGC	8 cut(s)	30, 848, 945, 990, 1058, 2196, 2400, 2403
BlpI	GCTNAGC	1 cut(s)	1161
BlsI	GCNGC	8 cut(s)	31, 849, 946, 991, 1059, 2197, 2401, 2404
BmcAI	AGTACT	1 cut(s)	1156
Bme1390I	CCNGG	1 cut(s)	1456
Bme18I	GGWCC	1 cut(s)	2038
BmeT110I	CYCGRG	1 cut(s)	1599
BmgT120I	GGNCC	3 cut(s)	1314, 1758, 2038
BmrFI	CCNGG	1 cut(s)	1456
BmrI	ACTGGG	1 cut(s)	932
BmsI	GCATC	1 cut(s)	2400
BmtI	GCTAGC	1 cut(s)	435
BmuI	ACTGGG	1 cut(s)	932
BpiI	GAAGAC	1 cut(s)	519
BpmI	CTGGAG	1 cut(s)	1943
Bpu10I	CCTNAGC	1 cut(s)	712
Bpu1102I	GCTNAGC	1 cut(s)	1161
BpuEI	CTTGAG	1 cut(s)	104
BsaAI	YACGTR	1 cut(s)	2342
BsaJI	CCNNGG	5 cut(s)	552, 1376, 1454, 1455, 1598
BsaWI	WCCGGW	2 cut(s)	1276, 1906
BsaXI	ACNNNNNCTCC	4 cut(s)	796, 826, 2331, 2361
Bsc4I	CCNNNNNNNGG	9 cut(s)	132, 636, 821, 1095, 1276, 1391, 1599, 1747, 2259
Bse118I	RCCGGY	1 cut(s)	25
Bse1I	ACTGG	6 cut(s)	193, 811, 927, 1703, 2048, 2264
Bse21I	CCTNAGG	2 cut(s)	1101, 2307
Bse3DI	GCAATG	3 cut(s)	1052, 1241, 1901
BseBI	CCWGG	1 cut(s)	1456
BseDI	CCNNGG	5 cut(s)	552, 1376, 1454, 1455, 1598
BseGI	GGATG	4 cut(s)	743, 1121, 1334, 2281
BseLI	CCNNNNNNNGG	9 cut(s)	132, 636, 821, 1095, 1276, 1391, 1599, 1747, 2259
BseMI	GCAATG	3 cut(s)	1052, 1241, 1901
BseMII	CTCAG	6 cut(s)	710, 726, 1152, 1698, 2012, 2248
BseNI	ACTGG	6 cut(s)	193, 811, 927, 1703, 2048, 2264
BseRI	GAGGAG	1 cut(s)	1958
BseSI	GKGCMC	1 cut(s)	2271
BseX3I	CGGCCG	1 cut(s)	1008
BseXI	GCAGC	4 cut(s)	931, 1001, 2207, 2411
BseYI	CCCAGC	2 cut(s)	1125, 1269
BsgI	GTGCAG	2 cut(s)	540, 1347
Bsh1236I	CGCG	1 cut(s)	23
Bsh1285I	CGRYCG	1 cut(s)	1011
BshFI	GGCC	8 cut(s)	125, 648, 1010, 1099, 1316, 1517, 1760, 2263
BshNI	GGYRCC	1 cut(s)	2248
BsiEI	CGRYCG	1 cut(s)	1011
BsiHKAI	GWGCWC	2 cut(s)	132, 2349
BsiHKCI	CYCGRG	1 cut(s)	1599
BsiSI	CCGG	3 cut(s)	26, 1277, 1907
BsiWI	CGTACG	1 cut(s)	57
BslFI	GGGAC	1 cut(s)	1345
BslI	CCNNNNNNNGG	9 cut(s)	132, 636, 821, 1095, 1276, 1391, 1599, 1747, 2259
BsmAI	GTCTC	3 cut(s)	47, 1038, 1295
BsmBI	CGTCTC	1 cut(s)	47
BsmFI	GGGAC	1 cut(s)	1345
BsmI	GAATGC	1 cut(s)	805
BsnI	GGCC	8 cut(s)	125, 648, 1010, 1099, 1316, 1517, 1760, 2263
BsoBI	CYCGRG	1 cut(s)	1599
Bsp1286I	GDGCHC	5 cut(s)	132, 1447, 1890, 2271, 2349
Bsp1407I	TGTACA	1 cut(s)	1898
Bsp1720I	GCTNAGC	1 cut(s)	1161
Bsp19I	CCATGG	1 cut(s)	1376
BspACI	CCGC	6 cut(s)	29, 363, 847, 1058, 1978, 2402
BspANI	GGCC	8 cut(s)	125, 648, 1010, 1099, 1316, 1517, 1760, 2263
BspCNI	CTCAG	6 cut(s)	711, 725, 1153, 1697, 2011, 2247
BspFNI	CGCG	1 cut(s)	23
BspMAI	CTGCAG	2 cut(s)	991, 2389
BspMI	ACCTGC	1 cut(s)	994
BspOI	GCTAGC	1 cut(s)	435
BspPI	GGATC	2 cut(s)	189, 1864
BspT107I	GGYRCC	1 cut(s)	2248
BsrDI	GCAATG	3 cut(s)	1052, 1241, 1901
BsrFI	RCCGGY	1 cut(s)	25
BsrGI	TGTACA	1 cut(s)	1898
BsrI	ACTGG	6 cut(s)	193, 811, 927, 1703, 2048, 2264
BssAI	RCCGGY	1 cut(s)	25
BssECI	CCNNGG	5 cut(s)	552, 1376, 1454, 1455, 1598
BssT1I	CCWWGG	2 cut(s)	552, 1376
Bst2UI	CCWGG	1 cut(s)	1456
Bst4CI	ACNGT	3 cut(s)	422, 741, 1674
Bst6I	CTCTTC	3 cut(s)	1167, 2178, 2208
BstAUI	TGTACA	1 cut(s)	1898
BstBAI	YACGTR	1 cut(s)	2342
BstC8I	GCNNGC	8 cut(s)	27, 327, 433, 466, 601, 694, 1248, 2069
BstDSI	CCRYGG	1 cut(s)	1376
BstENI	CCTNNNNNAGG	2 cut(s)	1093, 1389
BstF5I	GGATG	4 cut(s)	743, 1121, 1334, 2281
BstFNI	CGCG	1 cut(s)	23
BstHHI	GCGC	3 cut(s)	25, 146, 2399
BstMAI	GTCTC	3 cut(s)	47, 1038, 1295
BstMCI	CGRYCG	1 cut(s)	1011
BstMWI	GCNNNNNNNGC	3 cut(s)	29, 35, 1105
BstNI	CCWGG	1 cut(s)	1456
BstNSI	RCATGY	3 cut(s)	217, 794, 1589
BstSCI	CCNGG	1 cut(s)	1454
BstSFI	CTRYAG	3 cut(s)	459, 987, 2385
BstSLI	GKGCMC	1 cut(s)	2271
BstUI	CGCG	1 cut(s)	23
BstV1I	GCAGC	4 cut(s)	931, 1001, 2207, 2411
BstV2I	GAAGAC	1 cut(s)	519
BstX2I	RGATCY	2 cut(s)	194, 869
BstYI	RGATCY	2 cut(s)	194, 869
BstZI	CGGCCG	1 cut(s)	1008
Bsu36I	CCTNAGG	2 cut(s)	1101, 2307
BsuI	GTATCC	2 cut(s)	376, 1623
BsuRI	GGCC	8 cut(s)	125, 648, 1010, 1099, 1316, 1517, 1760, 2263
BtgI	CCRYGG	1 cut(s)	1376
BtsCI	GGATG	4 cut(s)	743, 1121, 1334, 2281
BtsI	GCAGTG	1 cut(s)	528
BtsIMutI	CAGTG	6 cut(s)	528, 716, 1146, 1854, 2222, 2271
BveI	ACCTGC	1 cut(s)	994
Cac8I	GCNNGC	8 cut(s)	27, 327, 433, 466, 601, 694, 1248, 2069
CaiI	CAGNNNCTG	1 cut(s)	947
CfoI	GCGC	3 cut(s)	25, 146, 2399
Cfr10I	RCCGGY	1 cut(s)	25
Cfr13I	GGNCC	3 cut(s)	1314, 1758, 2038
Csp6I	GTAC	8 cut(s)	58, 357, 492, 1038, 1155, 1263, 1899, 2249
CspCI	CAANNNNNGTGG	2 cut(s)	2187, 2222
CviQI	GTAC	8 cut(s)	58, 357, 492, 1038, 1155, 1263, 1899, 2249
EaeI	YGGCCR	1 cut(s)	1008
EagI	CGGCCG	1 cut(s)	1008
Eam1104I	CTCTTC	3 cut(s)	1167, 2178, 2208
EarI	CTCTTC	3 cut(s)	1167, 2178, 2208
Ecl136II	GAGCTC	1 cut(s)	130
EclXI	CGGCCG	1 cut(s)	1008
Eco130I	CCWWGG	2 cut(s)	552, 1376
Eco147I	AGGCCT	2 cut(s)	648, 1099
Eco24I	GRGCYC	3 cut(s)	132, 1447, 1890
Eco47I	GGWCC	1 cut(s)	2038
Eco52I	CGGCCG	1 cut(s)	1008
Eco53kI	GAGCTC	1 cut(s)	130
Eco57I	CTGAAG	3 cut(s)	351, 924, 1806
Eco81I	CCTNAGG	2 cut(s)	1101, 2307
Eco88I	CYCGRG	1 cut(s)	1599
EcoICRI	GAGCTC	1 cut(s)	130
EcoNI	CCTNNNNNAGG	2 cut(s)	1093, 1389
EcoO109I	RGGNCCY	1 cut(s)	2038
EcoRI	GAATTC	2 cut(s)	1289, 1604
EcoRII	CCWGG	1 cut(s)	1454
EcoT14I	CCWWGG	2 cut(s)	552, 1376
EcoT38I	GRGCYC	3 cut(s)	132, 1447, 1890
ErhI	CCWWGG	2 cut(s)	552, 1376
Esp3I	CGTCTC	1 cut(s)	47
FaqI	GGGAC	1 cut(s)	1345
FauNDI	CATATG	1 cut(s)	1071
FbaI	TGATCA	3 cut(s)	2107, 2137, 2376
Fnu4HI	GCNGC	8 cut(s)	30, 848, 945, 990, 1058, 2196, 2400, 2403
FokI	GGATG	4 cut(s)	730, 1108, 1321, 2268
FriOI	GRGCYC	3 cut(s)	132, 1447, 1890
Fsp4HI	GCNGC	8 cut(s)	30, 848, 945, 990, 1058, 2196, 2400, 2403
FspBI	CTAG	5 cut(s)	149, 432, 495, 873, 1229
GlaI	GCGC	3 cut(s)	24, 145, 2398
GluI	GCNGC	8 cut(s)	30, 848, 945, 990, 1058, 2196, 2400, 2403
GsaI	CCCAGC	2 cut(s)	1129, 1273
GsuI	CTGGAG	1 cut(s)	1943
HaeIII	GGCC	8 cut(s)	125, 648, 1010, 1099, 1316, 1517, 1760, 2263
HapII	CCGG	3 cut(s)	26, 1277, 1907
HhaI	GCGC	3 cut(s)	25, 146, 2399
Hin6I	GCGC	3 cut(s)	23, 144, 2397
HinP1I	GCGC	3 cut(s)	23, 144, 2397
HindIII	AAGCTT	1 cut(s)	597
HinfI	GANTC	5 cut(s)	257, 655, 880, 1225, 1500
HpaII	CCGG	3 cut(s)	26, 1277, 1907
HphI	GGTGA	7 cut(s)	156, 1078, 1471, 1685, 2068, 2322, 2386
Hpy166II	GTNNAC	3 cut(s)	572, 1265, 1451
Hpy188I	TCNGA	9 cut(s)	268, 316, 549, 1323, 1368, 1527, 1687, 2001, 2237
Hpy8I	GTNNAC	3 cut(s)	572, 1265, 1451
Hpy99I	CGWCG	5 cut(s)	37, 50, 110, 113, 1009
HpyAV	CCTTC	7 cut(s)	171, 628, 857, 1975, 2212, 2232, 2300
HpyCH4III	ACNGT	3 cut(s)	422, 741, 1674
HpyCH4IV	ACGT	2 cut(s)	56, 2341
HpyF10VI	GCNNNNNNNGC	3 cut(s)	29, 35, 1105
HpySE526I	ACGT	2 cut(s)	56, 2341
HspAI	GCGC	3 cut(s)	23, 144, 2397
KpnI	GGTACC	1 cut(s)	2252
KroI	GCCGGC	1 cut(s)	25
KroNI	GCCGGC	1 cut(s)	27
Ksp22I	TGATCA	3 cut(s)	2107, 2137, 2376
LmnI	GCTCC	4 cut(s)	76, 163, 1105, 2352
Lsp1109I	GCAGC	4 cut(s)	931, 1001, 2207, 2411
LweI	GCATC	1 cut(s)	2400
MaeI	CTAG	5 cut(s)	149, 432, 495, 873, 1229
MaeII	ACGT	2 cut(s)	56, 2341
MaeIII	GTNAC	4 cut(s)	218, 731, 931, 1588
MfeI	CAATTG	1 cut(s)	1802
MflI	RGATCY	2 cut(s)	194, 869
MhlI	GDGCHC	5 cut(s)	132, 1447, 1890, 2271, 2349
MlyI	GAGTC	1 cut(s)	664
MmeI	TCCRAC	1 cut(s)	2374
MreI	CGCCGGCG	1 cut(s)	25
MroNI	GCCGGC	1 cut(s)	25
MroXI	GAANNNNTTC	1 cut(s)	1025
MseI	TTAA	9 cut(s)	272, 338, 399, 413, 534, 762, 1200, 1214, 1679
MspA1I	CMGCKG	5 cut(s)	363, 944, 1921, 1978, 2402
MspI	CCGG	3 cut(s)	26, 1277, 1907
MspR9I	CCNGG	1 cut(s)	1456
MunI	CAATTG	1 cut(s)	1802
Mva1269I	GAATGC	1 cut(s)	805
MvaI	CCWGG	1 cut(s)	1456
MvnI	CGCG	1 cut(s)	23
MwoI	GCNNNNNNNGC	3 cut(s)	29, 35, 1105
NaeI	GCCGGC	1 cut(s)	27
NcoI	CCATGG	1 cut(s)	1376
NdeI	CATATG	1 cut(s)	1071
NgoMIV	GCCGGC	1 cut(s)	25
NheI	GCTAGC	1 cut(s)	431
NmeAIII	GCCGAG	3 cut(s)	47, 151, 1786
NmuCI	GTSAC	3 cut(s)	218, 731, 1588
NspI	RCATGY	3 cut(s)	217, 794, 1589
PasI	CCCWGGG	1 cut(s)	1455
PceI	AGGCCT	2 cut(s)	648, 1099
PciI	ACATGT	2 cut(s)	790, 1585
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	1 cut(s)	805
PdiI	GCCGGC	1 cut(s)	27
PdmI	GAANNNNTTC	1 cut(s)	1025
PfeI	GAWTC	4 cut(s)	257, 880, 1225, 1500
Pfl23II	CGTACG	1 cut(s)	57
PkrI	GCNGC	8 cut(s)	31, 849, 946, 991, 1059, 2197, 2401, 2404
PleI	GAGTC	1 cut(s)	663
PpsI	GAGTC	1 cut(s)	663
Ppu21I	YACGTR	1 cut(s)	2342
PpuMI	RGGWCCY	1 cut(s)	2038
PscI	ACATGT	2 cut(s)	790, 1585
Psp124BI	GAGCTC	1 cut(s)	132
Psp5II	RGGWCCY	1 cut(s)	2038
Psp6I	CCWGG	1 cut(s)	1454
PspFI	CCCAGC	2 cut(s)	1125, 1269
PspGI	CCWGG	1 cut(s)	1454
PspLI	CGTACG	1 cut(s)	57
PspPI	GGNCC	3 cut(s)	1314, 1758, 2038
PspPPI	RGGWCCY	1 cut(s)	2038
PstI	CTGCAG	2 cut(s)	991, 2389
PstNI	CAGNNNCTG	1 cut(s)	947
PsuI	RGATCY	2 cut(s)	194, 869
PvuII	CAGCTG	2 cut(s)	944, 1921
RsaI	GTAC	8 cut(s)	59, 358, 493, 1039, 1156, 1264, 1900, 2250
RsaNI	GTAC	8 cut(s)	58, 357, 492, 1038, 1155, 1263, 1899, 2249
SacI	GAGCTC	1 cut(s)	132
SaqAI	TTAA	9 cut(s)	272, 338, 399, 413, 534, 762, 1200, 1214, 1679
SatI	GCNGC	8 cut(s)	30, 848, 945, 990, 1058, 2196, 2400, 2403
Sau96I	GGNCC	3 cut(s)	1314, 1758, 2038
ScaI	AGTACT	1 cut(s)	1156
SchI	GAGTC	1 cut(s)	664
ScrFI	CCNGG	1 cut(s)	1456
SduI	GDGCHC	5 cut(s)	132, 1447, 1890, 2271, 2349
SfaNI	GCATC	1 cut(s)	2400
SfcI	CTRYAG	3 cut(s)	459, 987, 2385
SgrAI	CRCCGGYG	1 cut(s)	25
SinI	GGWCC	1 cut(s)	2038
SmlI	CTYRAG	1 cut(s)	119
SmoI	CTYRAG	1 cut(s)	119
SpeI	ACTAGT	1 cut(s)	494
SseBI	AGGCCT	2 cut(s)	648, 1099
SsiI	CCGC	6 cut(s)	29, 363, 847, 1058, 1978, 2402
SspMI	CTAG	5 cut(s)	149, 432, 495, 873, 1229
SstI	GAGCTC	1 cut(s)	132
StuI	AGGCCT	2 cut(s)	648, 1099
StyD4I	CCNGG	1 cut(s)	1454
StyI	CCWWGG	2 cut(s)	552, 1376
TaaI	ACNGT	3 cut(s)	422, 741, 1674
TaiI	ACGT	2 cut(s)	59, 2344
TaqI	TCGA	8 cut(s)	48, 84, 138, 207, 1004, 1207, 1494, 2113
TaqII	GACCGA	1 cut(s)	20
TatI	WGTACW	4 cut(s)	491, 1154, 1262, 1898
TauI	GCSGC	4 cut(s)	32, 850, 1060, 2405
TfiI	GAWTC	4 cut(s)	257, 880, 1225, 1500
Tru1I	TTAA	9 cut(s)	272, 338, 399, 413, 534, 762, 1200, 1214, 1679
Tru9I	TTAA	9 cut(s)	272, 338, 399, 413, 534, 762, 1200, 1214, 1679
TscAI	CASTG	6 cut(s)	528, 723, 1146, 1861, 2229, 2271
TseFI	GTSAC	3 cut(s)	218, 731, 1588
TseI	GCWGC	4 cut(s)	944, 989, 2195, 2399
Tsp45I	GTSAC	3 cut(s)	218, 731, 1588
TspDTI	ATGAA	6 cut(s)	603, 609, 1034, 1088, 1638, 1768
TspGWI	ACGGA	3 cut(s)	519, 1421, 2368
TspRI	CASTG	6 cut(s)	528, 723, 1146, 1861, 2229, 2271
VpaK11BI	GGWCC	1 cut(s)	2038
XagI	CCTNNNNNAGG	2 cut(s)	1093, 1389
XapI	RAATTY	8 cut(s)	1112, 1188, 1280, 1289, 1508, 1604, 1775, 2132
XbaI	TCTAGA	2 cut(s)	872, 1228
XceI	RCATGY	3 cut(s)	217, 794, 1589
XmnI	GAANNNNTTC	1 cut(s)	1025
XspI	CTAG	5 cut(s)	149, 432, 495, 873, 1229
ZrmI	AGTACT	1 cut(s)	1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.