Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0000750

Physical Location & Seq

Genomic Coordinates Reverse (-)

40616586 .. 40621554

Gene Structure 4969 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G026360.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGCCGCCGGCGACTGAGGAACCTAACACGACGGCGTCCTCGACGACGATGATGATCGACGAGATTTATGAGTTCTCGGCCCCGCGCTTCTTCGATTTCATCAAGGACGAGACCGACGATGACAAGATCAACGCCGAGCTCTGGTTCGACTCCGCCACAGCCTACGCCCCTTCCCCCTTCATGCCTAGAATCAAGACTGGTAGATCCTTTACAGTAGAGAGCATGTGCGACTTTACTGAAGCGGAAAATATGCAGAAGGTGAACCTTGAATCATCCTCAGCTGATGATGACTCTACGGAAACCAAGACTGAGTCGAAGATCACTCCTGAATTGAAGGAAGAAACAATTCCCAGGGAGGCTAAAGAAGAAAACAGTACAAGACAAGCCAATATGATTTCTGCTGATAAAGTCGAGGAAAAATGTACTATAGATGATGGAAGTGTTTTGGCCACTGCGGTATCTTTGAATAAGAGAACTGATATGACAAATGAGAAGAATAGTAATTCCGTAGCTTGTACTCCAAAACCACCCATGAATTATTCCAAGAAGGGAGATCTTCTGAGTGATTCCAAAAAAAACCCATCAGGGCTAAGGGGAAAAAGTCACTTGCACTCTTCTCAAACTAAGAATGTCAAACCATATACTACGAAAAGAGAAGAAAATGTGAAAAATATAACTGGCACACCCATGTTAGCTCAGGAGAATCAGGCAATTAAGCGGCAGAAGTTGGATGGAGGGAGATCTAGACAGATTCTTAATCCCAAACCCCAAACATTGCCCCACAAGTCAAAATTGGGTTATACCAGTGGCATGCATTCTTCATCGACTGCTGTTAAAACTCAAAAAGAGGACAGAAAGATGTATGTTCGGGAACCAGCAGCACCCTTTGTTTCAATGGCAGAAATGATGAACAGGTTCCAATCAAGTACCAGAGACTTGTCACTGCCACACAGTTCTCTTTCACATATGAAGCCCAAACTCACCTTAACAAGGCCGAAGGAGCCTGAATTTGAAACATCCCAGCGTGTCCGCTCAGTAAGAGTAAAGAGTACTGCCGAGCTTGAAGAAGAGATGATGGCCAAAATTCCAAAGTTTAAGGCTCGACCTGTAAACAAAAAGGTACTTGCCCCATCATTACCTACAAAGCCAAGAAGTACACCACAGCCACCTGAGTTTCAGGAATTCCATTTAGAGACTATGGCAAGGGCCAATCAAAATGCAGAAACATCCTCAGTAGCTTCAACTGATGTATCACGACAGAACAAACAATCGAAGCCCCATCATCTTACTGAACCGAAAACTCCAACGTTACATACATTATTGAGGGCCCGTCCACCCAGGGTGAAAACTTCATTGGAAATTGAGCAGGAAGAACTTGAAAAGATCCCAAAATTCAAAGCGAAGCCTCTGAATAAGAAGATATTTGAAAGTAAAGGAGATCTCGGGATGTTCTGTAATGCCAAGAAACAAGTGACTAAACCTCAGGAATTTCACTTTGCAACAAATGAAAGAATACCACCACCACCACCTTCAGCCATTCATGATCTATTTGATAAGTTGTCTTTGAACTCAGAGCCCCACCAGAATCCAATTCCAAGAAACACAATGCCAAATCCATTCCATCTCCATACAGAGGAAAGAGGAGCTGAGAAAGAAAGGAAATTTTTTACTGAAGTTATGCAGAAGCAATTGGAAGAAGAAAGAGCTAGGGTTCCCAAAGCTAATCCATATCCTTATACCACTGATTACCCTGTGATCCCACCAAAGCCAGAGCCCAAGGAATGCACAAAACCGGAACCTTTCCAACTAGAGAGCTTAGCGAGGCATGAGGAGGAAATGCAAAGGGAAATGGAAGAAAGACGGAGGATAGAGATGGAAGAAGCTCAGATGAGGGTTTTCAAGGCACAACCAATCTTGAAAGAGGACCCAATTCCAGTTCCCGAGAGAGTTCGCAAGCCTCTAACAGAAGTTCAAGAATTCAATCTGAATGTTAATAATCGGGCAGTGCACAGAGCAGAATTTGATCACAAGGTTAAGGAGAAAGAGATAATATATAAGAGATATAGAGAAGAGAGTGAAGCAGCAAGGATGTTAGAGGAAGAGAAAGCATTGAAACAACTGAGAAGGACGCTGGTACCCCATGCAAGGCCAGTGCCTAATTTTGAGCATCCATTCTGCCCCCAGAAGGAAACAACAAAAGCAAAGTCACCAAATCTGCGAGTTCTACAGCGGCAAGAAAGGCGAAGGATGATGATCAAATCCGCCACTTCCAGCGCTGCTTTGTACCCGTCTTCATCACTGCCAACTGAACAAAATTACTGTAAAGTTTCAGTTGTAATGAACCTGAATGTAATTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032886 GO:0033043 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050789 GO:0050794 GO:0051128 GO:0051225 GO:0051493 GO:0051726 GO:0060236 GO:0065007 GO:0070013 GO:0070507 GO:0070925 GO:0071840 GO:0090224 GO:0090307 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2 TPX2_importin

Protein Analysis

786

Amino Acids

90.1

Weight (kDa)

9.14

Isoelectric Point (pI)

60.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2_importin	PF12214	328 - 423	2.8e-29	Cell cycle regulated microtubule associated protein
TPX2_importin	PF12214	428 - 505	7.4e-18	Cell cycle regulated microtubule associated protein
TPX2	PF06886	660 - 732	9.2e-21	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03780 AT1G03780 AT1G03780
fragaria_vesca	FvH4_2g11956 FvH4_5g28370 FvH4_5g28401 FvH4_5g28402 FvH4_5g28440 FvH4_5g28450 FvH4_5g28450 FvH4_5g28471 FvH4_5g28480 FvH4_5g28561 FvH4_6g25610 FvH4_6g25610 FvH4_6g25690 FvH4_6g25731 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_c15g00040 FvH4_c17g00020 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090
malus_domestica	MD09G1243900.v1.1 MD17G1235800.v1.1
prunus_persica	Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1
pyrus_communis	pycom17g23880
rosa_chinensis	RchiOBHm_Chr2g0127601
rosa_laevigata	RLG00000018552 RLG00000018951
rosa_multiflora	Rmu_ssc0000200.1_g000020
rosa_roxburghii	Rroxscaffold_2G00116560 Rroxscaffold_2G00123480
rosa_rugosa	Rorug02G0272600
rosa_samantha	Rh2AG324600 Rh2BG333900 Rh2CG312100 Rh2DG352100
rosa_wichuraiana	Rw2G026360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2134
AccB1I	GGYRCC	1 cut(s)	2134
AccBSI	CCGCTC	1 cut(s)	1032
AccII	CGCG	1 cut(s)	85
AciI	CCGC	9 cut(s)	5, 83, 153, 242, 455, 718, 1030, 2230, 2262
AclI	AACGTT	1 cut(s)	1307
AclWI	GGATC	3 cut(s)	198, 1378, 1750
AcoI	YGGCCR	2 cut(s)	447, 1077
AcsI	RAATTY	8 cut(s)	1007, 1083, 1181, 1391, 1487, 1661, 1976, 2018
AcuI	CTGAAG	3 cut(s)	258, 1515, 1692
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	9 cut(s)	376, 424, 517, 928, 1051, 1122, 1156, 2136, 2285
AfeI	AGCGCT	1 cut(s)	2275
AfiI	CCNNNNNNNGG	5 cut(s)	141, 990, 1633, 2145, 2185
AjnI	CCWGG	2 cut(s)	350, 1337
AjuI	GAANNNNNNNTTGG	2 cut(s)	1337, 1369
Alw21I	GWGCWC	2 cut(s)	141, 2010
Alw26I	GTCTC	3 cut(s)	104, 927, 1187
Alw44I	GTGCAC	1 cut(s)	2006
AlwI	GGATC	3 cut(s)	198, 1378, 1750
Ama87I	CYCGRG	2 cut(s)	1442, 1940
Aor51HI	AGCGCT	1 cut(s)	2275
AoxI	GGCC	7 cut(s)	78, 447, 992, 1077, 1206, 1326, 2147
ApaI	GGGCCC	1 cut(s)	1330
ApaLI	GTGCAC	1 cut(s)	2006
ApeKI	GCWGC	3 cut(s)	878, 2081, 2276
ApoI	RAATTY	8 cut(s)	1007, 1083, 1181, 1391, 1487, 1661, 1976, 2018
ArsI	GACNNNNNNTTYG	2 cut(s)	2189, 2221
Asp700I	GAANNNNTTC	2 cut(s)	345, 914
Asp718I	GGTACC	1 cut(s)	2134
AspLEI	GCGC	2 cut(s)	87, 2276
AspS9I	GGNCC	5 cut(s)	79, 1206, 1326, 1327, 1924
AsuHPI	GGTGA	4 cut(s)	271, 973, 1354, 2199
AvaI	CYCGRG	2 cut(s)	1442, 1940
AvaII	GGWCC	1 cut(s)	1924
AxyI	CCTNAGG	1 cut(s)	1482
BaeGI	GKGCMC	2 cut(s)	1330, 2010
BaeI	ACNNNNGTAYC	6 cut(s)	441, 474, 1233, 1266, 2118, 2151
BalI	TGGCCA	2 cut(s)	449, 1079
BanI	GGYRCC	1 cut(s)	2134
BanII	GRGCYC	4 cut(s)	141, 1330, 1578, 1776
BbsI	GAAGAC	1 cut(s)	2283
Bbv12I	GWGCWC	2 cut(s)	141, 2010
BbvCI	CCTCAGC	1 cut(s)	277
BbvI	GCAGC	3 cut(s)	890, 2093, 2263
BccI	CCATC	8 cut(s)	428, 589, 725, 1069, 1138, 1287, 1629, 1867
BceAI	ACGGC	1 cut(s)	48
BciT130I	CCWGG	2 cut(s)	352, 1339
BclI	TGATCA	2 cut(s)	2023, 2253
BcoDI	GTCTC	3 cut(s)	104, 927, 1187
BfaI	CTAG	4 cut(s)	186, 744, 1707, 1808
BfmI	CTRYAG	2 cut(s)	426, 2225
BfoI	RGCGCY	1 cut(s)	2277
BglII	AGATCT	3 cut(s)	553, 740, 1438
BisI	GCNGC	6 cut(s)	5, 719, 879, 2082, 2231, 2277
BlpI	GCTNAGC	1 cut(s)	1816
BlsI	GCNGC	6 cut(s)	6, 720, 880, 2083, 2232, 2278
BmcAI	AGTACT	1 cut(s)	1051
Bme1390I	CCNGG	2 cut(s)	352, 1339
Bme18I	GGWCC	1 cut(s)	1924
BmeT110I	CYCGRG	2 cut(s)	1442, 1940
BmgT120I	GGNCC	5 cut(s)	79, 1206, 1326, 1327, 1924
BmrFI	CCNGG	2 cut(s)	352, 1339
BmsI	GCATC	1 cut(s)	2176
BpiI	GAAGAC	1 cut(s)	2283
Bpu10I	CCTNAGC	3 cut(s)	277, 590, 696
Bpu1102I	GCTNAGC	1 cut(s)	1816
BsaBI	GATNNNNATC	2 cut(s)	53, 2252
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	1 cut(s)	104
BsaJI	CCNNGG	5 cut(s)	350, 351, 1337, 1338, 1776
BsaWI	WCCGGW	1 cut(s)	1792
Bsc4I	CCNNNNNNNGG	5 cut(s)	141, 990, 1633, 2145, 2185
Bse118I	RCCGGY	1 cut(s)	7
Bse1I	ACTGG	5 cut(s)	202, 682, 804, 1934, 2150
Bse21I	CCTNAGG	1 cut(s)	1482
Bse3DI	GCAATG	1 cut(s)	773
Bse8I	GATNNNNATC	2 cut(s)	53, 2252
BseBI	CCWGG	2 cut(s)	352, 1339
BseDI	CCNNGG	5 cut(s)	350, 351, 1337, 1338, 1776
BseGI	GGATG	8 cut(s)	272, 736, 1016, 1226, 1452, 2094, 2167, 2253
BseJI	GATNNNNATC	2 cut(s)	53, 2252
BseLI	CCNNNNNNNGG	5 cut(s)	141, 990, 1633, 2145, 2185
BseMI	GCAATG	1 cut(s)	773
BseNI	ACTGG	5 cut(s)	202, 682, 804, 1934, 2150
BseRI	GAGGAG	2 cut(s)	1656, 1844
BseSI	GKGCMC	2 cut(s)	1330, 2010
BseXI	GCAGC	3 cut(s)	890, 2093, 2263
BseYI	CCCAGC	1 cut(s)	1020
Bsh1236I	CGCG	1 cut(s)	85
BshFI	GGCC	7 cut(s)	80, 449, 994, 1079, 1208, 1328, 2149
BshNI	GGYRCC	1 cut(s)	2134
BsiHKAI	GWGCWC	2 cut(s)	141, 2010
BsiHKCI	CYCGRG	2 cut(s)	1442, 1940
BsiSI	CCGG	2 cut(s)	8, 1793
BslI	CCNNNNNNNGG	5 cut(s)	141, 990, 1633, 2145, 2185
BsmAI	GTCTC	3 cut(s)	104, 927, 1187
BsmI	GAATGC	2 cut(s)	814, 1787
BsnI	GGCC	7 cut(s)	80, 449, 994, 1079, 1208, 1328, 2149
Bso31I	GGTCTC	1 cut(s)	104
BsoBI	CYCGRG	2 cut(s)	1442, 1940
Bsp120I	GGGCCC	1 cut(s)	1326
Bsp1286I	GDGCHC	5 cut(s)	141, 1330, 1578, 1776, 2010
Bsp1720I	GCTNAGC	1 cut(s)	1816
BspACI	CCGC	9 cut(s)	5, 83, 153, 242, 455, 718, 1030, 2230, 2262
BspANI	GGCC	7 cut(s)	80, 449, 994, 1079, 1208, 1328, 2149
BspFNI	CGCG	1 cut(s)	85
BspHI	TCATGA	1 cut(s)	1540
BspPI	GGATC	3 cut(s)	198, 1378, 1750
BspT107I	GGYRCC	1 cut(s)	2134
BspTNI	GGTCTC	1 cut(s)	104
BsrBI	CCGCTC	1 cut(s)	1032
BsrDI	GCAATG	1 cut(s)	773
BsrFI	RCCGGY	1 cut(s)	7
BsrI	ACTGG	5 cut(s)	202, 682, 804, 1934, 2150
BssAI	RCCGGY	1 cut(s)	7
BssECI	CCNNGG	5 cut(s)	350, 351, 1337, 1338, 1776
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	1 cut(s)	1776
Bst2UI	CCWGG	2 cut(s)	352, 1339
Bst4CI	ACNGT	4 cut(s)	214, 374, 953, 2321
Bst6I	CTCTTC	4 cut(s)	619, 1062, 2064, 2094
BstACI	GRCGYC	1 cut(s)	35
BstC8I	GCNNGC	3 cut(s)	9, 812, 1955
BstENI	CCTNNNNNAGG	1 cut(s)	988
BstF5I	GGATG	8 cut(s)	272, 736, 1016, 1226, 1452, 2094, 2167, 2253
BstFNI	CGCG	1 cut(s)	85
BstH2I	RGCGCY	1 cut(s)	2277
BstHHI	GCGC	2 cut(s)	87, 2276
BstMAI	GTCTC	3 cut(s)	104, 927, 1187
BstMWI	GCNNNNNNNGC	2 cut(s)	1000, 2239
BstNI	CCWGG	2 cut(s)	352, 1339
BstNSI	RCATGY	2 cut(s)	226, 814
BstSCI	CCNGG	2 cut(s)	350, 1337
BstSFI	CTRYAG	2 cut(s)	426, 2225
BstSLI	GKGCMC	2 cut(s)	1330, 2010
BstUI	CGCG	1 cut(s)	85
BstV1I	GCAGC	3 cut(s)	890, 2093, 2263
BstV2I	GAAGAC	1 cut(s)	2283
BstX2I	RGATCY	5 cut(s)	203, 553, 740, 1383, 1438
BstYI	RGATCY	5 cut(s)	203, 553, 740, 1383, 1438
Bsu36I	CCTNAGG	1 cut(s)	1482
BsuRI	GGCC	7 cut(s)	80, 449, 994, 1079, 1208, 1328, 2149
BtsCI	GGATG	8 cut(s)	272, 736, 1016, 1226, 1452, 2094, 2167, 2253
BtsI	GCAGTG	4 cut(s)	450, 941, 2010, 2297
BtsIMutI	CAGTG	7 cut(s)	450, 811, 941, 1740, 2010, 2157, 2297
Cac8I	GCNNGC	3 cut(s)	9, 812, 1955
CciI	TCATGA	1 cut(s)	1540
CfoI	GCGC	2 cut(s)	87, 2276
Cfr10I	RCCGGY	1 cut(s)	7
Cfr13I	GGNCC	5 cut(s)	79, 1206, 1326, 1327, 1924
CseI	GACGC	2 cut(s)	24, 2137
Csp6I	GTAC	9 cut(s)	375, 423, 516, 927, 1050, 1121, 1155, 2135, 2284
CviAII	CATG	8 cut(s)	181, 223, 532, 688, 811, 1541, 1826, 2141
CviQI	GTAC	9 cut(s)	375, 423, 516, 927, 1050, 1121, 1155, 2135, 2284
EaeI	YGGCCR	2 cut(s)	447, 1077
Eam1104I	CTCTTC	4 cut(s)	619, 1062, 2064, 2094
EarI	CTCTTC	4 cut(s)	619, 1062, 2064, 2094
EciI	GGCGGA	2 cut(s)	142, 2251
Ecl136II	GAGCTC	1 cut(s)	139
Eco130I	CCWWGG	1 cut(s)	1776
Eco24I	GRGCYC	4 cut(s)	141, 1330, 1578, 1776
Eco31I	GGTCTC	1 cut(s)	104
Eco47I	GGWCC	1 cut(s)	1924
Eco47III	AGCGCT	1 cut(s)	2275
Eco53kI	GAGCTC	1 cut(s)	139
Eco57I	CTGAAG	3 cut(s)	258, 1515, 1692
Eco81I	CCTNAGG	1 cut(s)	1482
Eco88I	CYCGRG	2 cut(s)	1442, 1940
EcoICRI	GAGCTC	1 cut(s)	139
EcoNI	CCTNNNNNAGG	1 cut(s)	988
EcoO109I	RGGNCCY	2 cut(s)	1326, 1924
EcoRI	GAATTC	2 cut(s)	1181, 1976
EcoRII	CCWGG	2 cut(s)	350, 1337
EcoT14I	CCWWGG	1 cut(s)	1776
EcoT22I	ATGCAT	1 cut(s)	816
EcoT38I	GRGCYC	4 cut(s)	141, 1330, 1578, 1776
ErhI	CCWWGG	1 cut(s)	1776
FaeI	CATG	8 cut(s)	184, 226, 535, 691, 814, 1544, 1829, 2144
FatI	CATG	8 cut(s)	180, 222, 531, 687, 810, 1540, 1825, 2140
FauI	CCCGC	1 cut(s)	90
FauNDI	CATATG	1 cut(s)	966
FbaI	TGATCA	2 cut(s)	2023, 2253
Fnu4HI	GCNGC	6 cut(s)	5, 719, 879, 2082, 2231, 2277
FokI	GGATG	8 cut(s)	259, 743, 1003, 1213, 1459, 2101, 2154, 2260
FriOI	GRGCYC	4 cut(s)	141, 1330, 1578, 1776
Fsp4HI	GCNGC	6 cut(s)	5, 719, 879, 2082, 2231, 2277
FspBI	CTAG	4 cut(s)	186, 744, 1707, 1808
GlaI	GCGC	2 cut(s)	86, 2275
GluI	GCNGC	6 cut(s)	5, 719, 879, 2082, 2231, 2277
GsaI	CCCAGC	1 cut(s)	1024
HaeII	RGCGCY	1 cut(s)	2277
HaeIII	GGCC	7 cut(s)	80, 449, 994, 1079, 1208, 1328, 2149
HapII	CCGG	2 cut(s)	8, 1793
HgaI	GACGC	2 cut(s)	24, 2137
HhaI	GCGC	2 cut(s)	87, 2276
Hin1I	GRCGYC	1 cut(s)	35
Hin1II	CATG	8 cut(s)	184, 226, 535, 691, 814, 1544, 1829, 2144
Hin6I	GCGC	2 cut(s)	85, 2274
HinP1I	GCGC	2 cut(s)	85, 2274
HinfI	GANTC	9 cut(s)	149, 189, 269, 290, 311, 566, 703, 751, 1585
HpaII	CCGG	2 cut(s)	8, 1793
HphI	GGTGA	4 cut(s)	271, 973, 1354, 2199
Hpy166II	GTNNAC	5 cut(s)	262, 1111, 1157, 1334, 2008
Hpy188I	TCNGA	5 cut(s)	561, 1410, 1573, 1887, 1986
Hpy8I	GTNNAC	5 cut(s)	262, 1111, 1157, 1334, 2008
Hpy99I	CGWCG	5 cut(s)	34, 46, 49, 62, 119
HpyCH4III	ACNGT	4 cut(s)	214, 374, 953, 2321
HpyCH4IV	ACGT	1 cut(s)	1307
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1000, 2239
HpySE526I	ACGT	1 cut(s)	1307
Hsp92I	GRCGYC	1 cut(s)	35
Hsp92II	CATG	8 cut(s)	184, 226, 535, 691, 814, 1544, 1829, 2144
HspAI	GCGC	2 cut(s)	85, 2274
KpnI	GGTACC	1 cut(s)	2138
KroI	GCCGGC	1 cut(s)	7
KroNI	GCCGGC	1 cut(s)	9
Ksp22I	TGATCA	2 cut(s)	2023, 2253
LmnI	GCTCC	2 cut(s)	1000, 1643
Lsp1109I	GCAGC	3 cut(s)	890, 2093, 2263
LweI	GCATC	1 cut(s)	2176
MaeI	CTAG	4 cut(s)	186, 744, 1707, 1808
MaeII	ACGT	1 cut(s)	1307
MaeIII	GTNAC	5 cut(s)	602, 939, 1308, 1471, 2205
MbiI	CCGCTC	1 cut(s)	1032
MfeI	CAATTG	1 cut(s)	1688
MflI	RGATCY	5 cut(s)	203, 553, 740, 1383, 1438
MhlI	GDGCHC	5 cut(s)	141, 1330, 1578, 1776, 2010
MlsI	TGGCCA	2 cut(s)	449, 1079
MluNI	TGGCCA	2 cut(s)	449, 1079
MlyI	GAGTC	3 cut(s)	143, 284, 320
MmeI	TCCRAC	3 cut(s)	708, 1328, 1828
Mox20I	TGGCCA	2 cut(s)	449, 1079
Mph1103I	ATGCAT	1 cut(s)	816
MreI	CGCCGGCG	1 cut(s)	7
MroNI	GCCGGC	1 cut(s)	7
MroXI	GAANNNNTTC	2 cut(s)	345, 914
MscI	TGGCCA	2 cut(s)	449, 1079
MseI	TTAA	7 cut(s)	714, 756, 834, 986, 1095, 1992, 2034
MslI	CAYNNNNRTG	1 cut(s)	686
Msp20I	TGGCCA	2 cut(s)	449, 1079
MspA1I	CMGCKG	2 cut(s)	281, 2230
MspI	CCGG	2 cut(s)	8, 1793
MspR9I	CCNGG	2 cut(s)	352, 1339
MunI	CAATTG	1 cut(s)	1688
Mva1269I	GAATGC	2 cut(s)	814, 1787
MvaI	CCWGG	2 cut(s)	352, 1339
MvnI	CGCG	1 cut(s)	85
MwoI	GCNNNNNNNGC	2 cut(s)	1000, 2239
NaeI	GCCGGC	1 cut(s)	9
NdeI	CATATG	1 cut(s)	966
NgoMIV	GCCGGC	1 cut(s)	7
NlaIII	CATG	8 cut(s)	184, 226, 535, 691, 814, 1544, 1829, 2144
NmeAIII	GCCGAG	3 cut(s)	56, 160, 1081
NmuCI	GTSAC	4 cut(s)	602, 939, 1471, 2205
NsiI	ATGCAT	1 cut(s)	816
NspI	RCATGY	2 cut(s)	226, 814
PaeI	GCATGC	1 cut(s)	814
PagI	TCATGA	1 cut(s)	1540
PasI	CCCWGGG	2 cut(s)	351, 1338
PcsI	WCGNNNNNNNCGW	2 cut(s)	38, 114
PctI	GAATGC	2 cut(s)	814, 1787
PdiI	GCCGGC	1 cut(s)	9
PdmI	GAANNNNTTC	2 cut(s)	345, 914
PfeI	GAWTC	6 cut(s)	189, 269, 566, 703, 751, 1585
PflFI	GACNNNGTC	2 cut(s)	34, 310
PkrI	GCNGC	6 cut(s)	6, 720, 880, 2083, 2232, 2278
PleI	GAGTC	3 cut(s)	143, 284, 319
PpsI	GAGTC	3 cut(s)	143, 284, 319
PpuMI	RGGWCCY	1 cut(s)	1924
Psp124BI	GAGCTC	1 cut(s)	141
Psp1406I	AACGTT	1 cut(s)	1307
Psp5II	RGGWCCY	1 cut(s)	1924
Psp6I	CCWGG	2 cut(s)	350, 1337
PspFI	CCCAGC	1 cut(s)	1020
PspGI	CCWGG	2 cut(s)	350, 1337
PspOMI	GGGCCC	1 cut(s)	1326
PspPI	GGNCC	5 cut(s)	79, 1206, 1326, 1327, 1924
PspPPI	RGGWCCY	1 cut(s)	1924
PsuI	RGATCY	5 cut(s)	203, 553, 740, 1383, 1438
PsyI	GACNNNGTC	2 cut(s)	34, 310
PvuII	CAGCTG	1 cut(s)	281
RsaI	GTAC	9 cut(s)	376, 424, 517, 928, 1051, 1122, 1156, 2136, 2285
RsaNI	GTAC	9 cut(s)	375, 423, 516, 927, 1050, 1121, 1155, 2135, 2284
RseI	CAYNNNNRTG	1 cut(s)	686
SacI	GAGCTC	1 cut(s)	141
SaqAI	TTAA	7 cut(s)	714, 756, 834, 986, 1095, 1992, 2034
SatI	GCNGC	6 cut(s)	5, 719, 879, 2082, 2231, 2277
Sau96I	GGNCC	5 cut(s)	79, 1206, 1326, 1327, 1924
ScaI	AGTACT	1 cut(s)	1051
SchI	GAGTC	3 cut(s)	143, 284, 320
ScrFI	CCNGG	2 cut(s)	352, 1339
SduI	GDGCHC	5 cut(s)	141, 1330, 1578, 1776, 2010
SfaNI	GCATC	1 cut(s)	2176
SfcI	CTRYAG	2 cut(s)	426, 2225
SgrAI	CRCCGGYG	1 cut(s)	7
SinI	GGWCC	1 cut(s)	1924
SmiMI	CAYNNNNRTG	1 cut(s)	686
SphI	GCATGC	1 cut(s)	814
SsiI	CCGC	9 cut(s)	5, 83, 153, 242, 455, 718, 1030, 2230, 2262
SspMI	CTAG	4 cut(s)	186, 744, 1707, 1808
SstI	GAGCTC	1 cut(s)	141
StyD4I	CCNGG	2 cut(s)	350, 1337
StyI	CCWWGG	1 cut(s)	1776
TaaI	ACNGT	4 cut(s)	214, 374, 953, 2321
TaiI	ACGT	1 cut(s)	1310
TaqI	TCGA	9 cut(s)	41, 57, 93, 147, 314, 411, 824, 1102, 1271
TaqII	GACCGA	1 cut(s)	128
TatI	WGTACW	5 cut(s)	374, 422, 515, 1049, 1154
TauI	GCSGC	3 cut(s)	7, 721, 2233
TfiI	GAWTC	6 cut(s)	189, 269, 566, 703, 751, 1585
Tru1I	TTAA	7 cut(s)	714, 756, 834, 986, 1095, 1992, 2034
Tru9I	TTAA	7 cut(s)	714, 756, 834, 986, 1095, 1992, 2034
TscAI	CASTG	7 cut(s)	457, 811, 948, 1747, 2010, 2157, 2304
TseFI	GTSAC	4 cut(s)	602, 939, 1471, 2205
TseI	GCWGC	3 cut(s)	878, 2081, 2276
Tsp45I	GTSAC	4 cut(s)	602, 939, 1471, 2205
TspGWI	ACGGA	3 cut(s)	311, 496, 1876
TspRI	CASTG	7 cut(s)	457, 811, 948, 1747, 2010, 2157, 2304
Tth111I	GACNNNGTC	2 cut(s)	34, 310
VneI	GTGCAC	1 cut(s)	2006
VpaK11BI	GGWCC	1 cut(s)	1924
XagI	CCTNNNNNAGG	1 cut(s)	988
XapI	RAATTY	8 cut(s)	1007, 1083, 1181, 1391, 1487, 1661, 1976, 2018
XbaI	TCTAGA	1 cut(s)	743
XceI	RCATGY	2 cut(s)	226, 814
XmnI	GAANNNNTTC	2 cut(s)	345, 914
XspI	CTAG	4 cut(s)	186, 744, 1707, 1808
ZrmI	AGTACT	1 cut(s)	1051
Zsp2I	ATGCAT	1 cut(s)	816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.