Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000750

Physical Location & Seq

Genomic Coordinates Reverse (-)

43373053 .. 43377842

Gene Structure 4790 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000018951

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2271 bp

ATGCCGCCGGCGACGGAGGAACCTAACACGACGGCGTCCTCAACGATGATGATCGACGAGATTTATGAGTTCTCGGCCCCGCGCTTCTTCGATTTCATCAAGGACGAGACCGACGATGAAAAGATCAAGACTGGTAGACCCTTTACAGTAGAGAGCATGTGCGACTTTACTGAAGCAGAAAATATGCAGAAGAACCTTGAGTCATCCTCAGCTGATGATGATTCTACAGAAACCAAGACTGAATCGAAGATCACTTCTGAATTGAAGGAAGAAAGAATTCCCAGGGAGGCTAAAGAAGAAAACAGTGCAAGACAAGCCAACATGATTTCTGCTGATAAAGTAGAGGAAAAATGTACTCTAGATGATGGAGGTGTTTTGGCCACTGCAGTATCTTTGAATAAGAGAACTGATATGACAAATGAGAAGGAGAATAGTAATTCCGTAGCTTGTACTCCAAAACCACCCATGAATTATTCCAAGAAGGGAGATCTTTTGAGTGATTCCAAAAAGCACCAGACAGCTAAAAAGATATCAAGTATGGTTAGAAACCCATCAGGGCTAAGAGGAAAAAGTCACTTGCACTCTTCACAAACTAAGAATGTCAAACCGTATACTATGAAAAGAGAAGAAAATGTGAAAAATATAACTGGCACACCCATGTTAGCTCAGGAGAATCAGGCAATTAAGCGGCAGAAATTGGATGGAGGGAGATCTAGACAGATTCTTAATCCCAAACCCCAAACACTGCCCCACAAGTCAAAATTGGGTTATACCAGTGGCATGCATTCTTCAACTGCTGTTAAAACTCAAAAAGAGGACAGAAAGATGTATGTTCGGGAACCAGCAGCACCCTTTGTTTCAATGGCAGAAATGATGAACAGGTTCCAATCAAGTACCAGAGACTTGTCACTGCCACACAGTTCTCTTTCACATATGAAGCCCAAACTCACCTTAACAAGGCCGAAGGAGCCTGAATTTGAAACATCCCAGCGTGTCCGCTCAGTAAGAGTAAAGAGTACTGCCGAGCTTGAAGAAGAGATGATGGCCAAAATTCCGAAGTTTAAGGCTCGACCTGTAAACAAAAAGATTCTTGAAGCGCCATCATTACCTGCAATGCCAAGAAGTACACCACAGCCACCTGAGTTTCAGGAATTCCATTTAGAGACTATGGCGAGGGCCAATCAAAATGCAGAAACATCCTCAGTAGCTTCAACTGATGTATCACGACGGAACAAACAATCGAAGCCCCATCATCTTACTGAACCTAAAACTCCAACGTTACATACATTATTGAGGGCCCGTCCACCCAGGGTGAAAACTTCATTGGAAATTGAGCAGGAAGAACTTGAAAAGATCCCAAAATTCAAAGCGAAGCCTCTGAATAAGAAGATATTTGAAAGTAAAGGAGATCTCGGGATGTTCTGTAATGCCAAGAAACATGTGACTAAACCTCAGGAATTTCACTTTGCAACAAATGAAAGAATACCACCACCACCACCTTCAGCCATTCATGATCTATTTGATAAGTTGTCTTTGAACTCAGAGCCCCACCAGAATCCAATTCCAAGAAACACAATGCCAAATCCATTCCATCTGCATACAGAGGAAAGAGGAGCTGAGAAAGAACAGAAATTTTTTACTGAAGTTATGCAGAAGCAATTGGAAGAAGAAAGAGCTAGGGTTCCCAAAGCTAATCCATATCCTTATACCACTGATTACCCTGTGATCCCACCCAAGCCAGAGCCCAAGGAATGCACAAAACCGGAACCTTTCCAACTAGAGAGCTTAGCGAGGCATGAGGAGGAAATGCAAAGGGAAATGGAAGAAAGACGGAGGATAGAGATGGAAGAAGCTCAGATGAGGGTTTTCAAGGCACAACCAATCTTGAAAGAGGACCCAATTCCAGTTCCCGAGAGAGTTCGCAAGCCTCTAACAGAAGTTCAAGAATTCAATCTGAATGTTAATAATCGGGCAGTGCACAGAGCAGAATTTGATCACAAGGTTAAGGAGAAAGAGATAATATATAAGAGATATAGAGAAGAGAGTGAAGCAGCAAGGATGTTAGAGGAAGAGAAAGCATTGAAACAACTGAGAAGGACGCTGGTACCTCATGCAAGGCCAGTGCCTAATTTTGAGCATCCATTCTGCCCCCAGAAGTGTCCAAGGGAAACAACAAAAGCAAAGTCACCAAATCTGCGAGTTCTACAGCGGCAAGAAAGGCGAAGGATGATGGTGAAATCCGCCACGAGTGCTGCTGCCCAGATGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032886 GO:0033043 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050789 GO:0050794 GO:0051128 GO:0051225 GO:0051493 GO:0051726 GO:0060236 GO:0065007 GO:0070013 GO:0070507 GO:0070925 GO:0071840 GO:0090224 GO:0090307 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2 TPX2_importin

Protein Analysis

757

Amino Acids

86.96

Weight (kDa)

9.3

Isoelectric Point (pI)

59.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2_importin	PF12214	317 - 412	1.3e-31	Cell cycle regulated microtubule associated protein
TPX2_importin	PF12214	418 - 495	9.2e-18	Cell cycle regulated microtubule associated protein
TPX2	PF06886	650 - 725	8e-21	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03780 AT1G03780 AT1G03780
fragaria_vesca	FvH4_2g11956 FvH4_5g28370 FvH4_5g28401 FvH4_5g28402 FvH4_5g28440 FvH4_5g28450 FvH4_5g28450 FvH4_5g28471 FvH4_5g28480 FvH4_5g28561 FvH4_6g25610 FvH4_6g25610 FvH4_6g25690 FvH4_6g25731 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_c15g00040 FvH4_c17g00020 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090
malus_domestica	MD09G1243900.v1.1 MD17G1235800.v1.1
prunus_persica	Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1
pyrus_communis	pycom17g23880
rosa_chinensis	RchiOBHm_Chr2g0127601
rosa_laevigata	RLG00000018552 RLG00000018951
rosa_multiflora	Rmu_ssc0000200.1_g000020
rosa_roxburghii	Rroxscaffold_2G00116560 Rroxscaffold_2G00123480
rosa_rugosa	Rorug02G0272600
rosa_samantha	Rh2AG324600 Rh2BG333900 Rh2CG312100 Rh2DG352100
rosa_wichuraiana	Rw2G026360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1117
Acc65I	GGTACC	1 cut(s)	2104
AccB1I	GGYRCC	1 cut(s)	2104
AccBSI	CCGCTC	1 cut(s)	999
AccI	GTMKAC	2 cut(s)	136, 611
AccII	CGCG	1 cut(s)	82
AciI	CCGC	6 cut(s)	5, 80, 688, 997, 2209, 2241
AclI	AACGTT	1 cut(s)	1277
AclWI	GGATC	2 cut(s)	1348, 1720
AcoI	YGGCCR	2 cut(s)	378, 1044
AcsI	RAATTY	9 cut(s)	276, 974, 1050, 1151, 1361, 1457, 1631, 1946, 1988
AcuI	CTGAAG	3 cut(s)	192, 1485, 1662
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	6 cut(s)	355, 451, 895, 1018, 1126, 2106
AfiI	CCNNNNNNNGG	1 cut(s)	957
AflIII	ACRYGT	1 cut(s)	1438
AjnI	CCWGG	2 cut(s)	281, 1307
AjuI	GAANNNNNNNTTGG	2 cut(s)	1307, 1339
Alw21I	GWGCWC	1 cut(s)	1980
Alw26I	GTCTC	3 cut(s)	101, 894, 1157
Alw44I	GTGCAC	1 cut(s)	1976
AlwI	GGATC	2 cut(s)	1348, 1720
Ama87I	CYCGRG	2 cut(s)	1412, 1910
AoxI	GGCC	7 cut(s)	75, 378, 959, 1044, 1176, 1296, 2117
ApaI	GGGCCC	1 cut(s)	1300
ApaLI	GTGCAC	1 cut(s)	1976
ApeKI	GCWGC	4 cut(s)	845, 2051, 2252, 2255
ApoI	RAATTY	9 cut(s)	276, 974, 1050, 1151, 1361, 1457, 1631, 1946, 1988
ArsI	GACNNNNNNTTYG	2 cut(s)	2168, 2200
Asp700I	GAANNNNTTC	2 cut(s)	276, 881
Asp718I	GGTACC	1 cut(s)	2104
AspLEI	GCGC	2 cut(s)	84, 1099
AspS9I	GGNCC	5 cut(s)	76, 1176, 1296, 1297, 1894
AsuHPI	GGTGA	4 cut(s)	940, 1324, 2178, 2245
AvaI	CYCGRG	2 cut(s)	1412, 1910
AvaII	GGWCC	1 cut(s)	1894
AxyI	CCTNAGG	1 cut(s)	1452
BaeGI	GKGCMC	2 cut(s)	1300, 1980
BaeI	ACNNNNGTAYC	6 cut(s)	372, 405, 1203, 1236, 2088, 2121
BalI	TGGCCA	2 cut(s)	380, 1046
BanI	GGYRCC	1 cut(s)	2104
BanII	GRGCYC	3 cut(s)	1300, 1548, 1746
BauI	CACGAG	1 cut(s)	2245
Bbv12I	GWGCWC	1 cut(s)	1980
BbvCI	CCTCAGC	1 cut(s)	208
BbvI	GCAGC	4 cut(s)	857, 2063, 2239, 2242
BccI	CCATC	9 cut(s)	359, 559, 695, 1036, 1108, 1257, 1599, 1837, 2224
BceAI	ACGGC	1 cut(s)	48
BciT130I	CCWGG	2 cut(s)	283, 1309
BclI	TGATCA	1 cut(s)	1993
BcoDI	GTCTC	3 cut(s)	101, 894, 1157
BfaI	CTAG	4 cut(s)	359, 714, 1677, 1778
BfmI	CTRYAG	3 cut(s)	225, 384, 2204
BfoI	RGCGCY	1 cut(s)	1100
BfuAI	ACCTGC	1 cut(s)	1117
BglII	AGATCT	3 cut(s)	487, 710, 1408
BisI	GCNGC	7 cut(s)	5, 689, 846, 2052, 2210, 2253, 2256
BlpI	GCTNAGC	1 cut(s)	1786
BlsI	GCNGC	7 cut(s)	6, 690, 847, 2053, 2211, 2254, 2257
BmcAI	AGTACT	1 cut(s)	1018
Bme1390I	CCNGG	2 cut(s)	283, 1309
Bme18I	GGWCC	1 cut(s)	1894
BmeT110I	CYCGRG	2 cut(s)	1412, 1910
BmgT120I	GGNCC	5 cut(s)	76, 1176, 1296, 1297, 1894
BmrFI	CCNGG	2 cut(s)	283, 1309
BmsI	GCATC	1 cut(s)	2146
BplI	GAGNNNNNCTC	2 cut(s)	191, 223
Bpu10I	CCTNAGC	2 cut(s)	208, 666
Bpu1102I	GCTNAGC	1 cut(s)	1786
BpuEI	CTTGAG	1 cut(s)	218
BsaBI	GATNNNNATC	1 cut(s)	50
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	1 cut(s)	101
BsaJI	CCNNGG	6 cut(s)	281, 282, 1307, 1308, 1746, 2162
BsaWI	WCCGGW	1 cut(s)	1762
Bsc4I	CCNNNNNNNGG	1 cut(s)	957
Bse118I	RCCGGY	1 cut(s)	7
Bse1I	ACTGG	5 cut(s)	136, 652, 774, 1904, 2120
Bse21I	CCTNAGG	1 cut(s)	1452
Bse3DI	GCAATG	1 cut(s)	1119
Bse8I	GATNNNNATC	1 cut(s)	50
BseBI	CCWGG	2 cut(s)	283, 1309
BseDI	CCNNGG	6 cut(s)	281, 282, 1307, 1308, 1746, 2162
BseGI	GGATG	8 cut(s)	203, 706, 983, 1196, 1422, 2064, 2137, 2232
BseJI	GATNNNNATC	1 cut(s)	50
BseLI	CCNNNNNNNGG	1 cut(s)	957
BseMI	GCAATG	1 cut(s)	1119
BseNI	ACTGG	5 cut(s)	136, 652, 774, 1904, 2120
BseRI	GAGGAG	2 cut(s)	1626, 1814
BseSI	GKGCMC	2 cut(s)	1300, 1980
BseXI	GCAGC	4 cut(s)	857, 2063, 2239, 2242
BseYI	CCCAGC	1 cut(s)	987
Bsh1236I	CGCG	1 cut(s)	82
BshFI	GGCC	7 cut(s)	77, 380, 961, 1046, 1178, 1298, 2119
BshNI	GGYRCC	1 cut(s)	2104
BsiHKAI	GWGCWC	1 cut(s)	1980
BsiHKCI	CYCGRG	2 cut(s)	1412, 1910
BsiSI	CCGG	2 cut(s)	8, 1763
BslI	CCNNNNNNNGG	1 cut(s)	957
BsmAI	GTCTC	3 cut(s)	101, 894, 1157
BsmI	GAATGC	2 cut(s)	784, 1757
BsnI	GGCC	7 cut(s)	77, 380, 961, 1046, 1178, 1298, 2119
Bso31I	GGTCTC	1 cut(s)	101
BsoBI	CYCGRG	2 cut(s)	1412, 1910
Bsp120I	GGGCCC	1 cut(s)	1296
Bsp1286I	GDGCHC	4 cut(s)	1300, 1548, 1746, 1980
Bsp1720I	GCTNAGC	1 cut(s)	1786
BspACI	CCGC	6 cut(s)	5, 80, 688, 997, 2209, 2241
BspANI	GGCC	7 cut(s)	77, 380, 961, 1046, 1178, 1298, 2119
BspFNI	CGCG	1 cut(s)	82
BspHI	TCATGA	1 cut(s)	1510
BspMAI	CTGCAG	1 cut(s)	388
BspMI	ACCTGC	1 cut(s)	1117
BspPI	GGATC	2 cut(s)	1348, 1720
BspT107I	GGYRCC	1 cut(s)	2104
BspTNI	GGTCTC	1 cut(s)	101
BsrBI	CCGCTC	1 cut(s)	999
BsrDI	GCAATG	1 cut(s)	1119
BsrFI	RCCGGY	1 cut(s)	7
BsrI	ACTGG	5 cut(s)	136, 652, 774, 1904, 2120
BssAI	RCCGGY	1 cut(s)	7
BssECI	CCNNGG	6 cut(s)	281, 282, 1307, 1308, 1746, 2162
BssNAI	GTATAC	1 cut(s)	612
BssNI	GRCGYC	1 cut(s)	35
BssSI	CACGAG	1 cut(s)	2245
BssT1I	CCWWGG	2 cut(s)	1746, 2162
Bst1107I	GTATAC	1 cut(s)	612
Bst2BI	CACGAG	1 cut(s)	2245
Bst2UI	CCWGG	2 cut(s)	283, 1309
Bst4CI	ACNGT	4 cut(s)	148, 305, 609, 920
Bst6I	CTCTTC	4 cut(s)	589, 1029, 2034, 2064
BstACI	GRCGYC	1 cut(s)	35
BstC8I	GCNNGC	3 cut(s)	9, 782, 1925
BstENI	CCTNNNNNAGG	1 cut(s)	955
BstF5I	GGATG	8 cut(s)	203, 706, 983, 1196, 1422, 2064, 2137, 2232
BstFNI	CGCG	1 cut(s)	82
BstH2I	RGCGCY	1 cut(s)	1100
BstHHI	GCGC	2 cut(s)	84, 1099
BstMAI	GTCTC	3 cut(s)	101, 894, 1157
BstMWI	GCNNNNNNNGC	4 cut(s)	314, 967, 2218, 2249
BstNI	CCWGG	2 cut(s)	283, 1309
BstNSI	RCATGY	3 cut(s)	160, 784, 1442
BstSCI	CCNGG	2 cut(s)	281, 1307
BstSFI	CTRYAG	3 cut(s)	225, 384, 2204
BstSLI	GKGCMC	2 cut(s)	1300, 1980
BstUI	CGCG	1 cut(s)	82
BstV1I	GCAGC	4 cut(s)	857, 2063, 2239, 2242
BstX2I	RGATCY	4 cut(s)	487, 710, 1353, 1408
BstYI	RGATCY	4 cut(s)	487, 710, 1353, 1408
BstZ17I	GTATAC	1 cut(s)	612
Bsu36I	CCTNAGG	1 cut(s)	1452
BsuRI	GGCC	7 cut(s)	77, 380, 961, 1046, 1178, 1298, 2119
BtsCI	GGATG	8 cut(s)	203, 706, 983, 1196, 1422, 2064, 2137, 2232
BtsI	GCAGTG	4 cut(s)	381, 743, 908, 1980
BtsIMutI	CAGTG	8 cut(s)	310, 381, 743, 781, 908, 1710, 1980, 2127
BveI	ACCTGC	1 cut(s)	1117
Cac8I	GCNNGC	3 cut(s)	9, 782, 1925
CciI	TCATGA	1 cut(s)	1510
CfoI	GCGC	2 cut(s)	84, 1099
Cfr10I	RCCGGY	1 cut(s)	7
Cfr13I	GGNCC	5 cut(s)	76, 1176, 1296, 1297, 1894
CseI	GACGC	2 cut(s)	24, 2107
Csp6I	GTAC	6 cut(s)	354, 450, 894, 1017, 1125, 2105
CviAII	CATG	9 cut(s)	157, 322, 466, 658, 781, 1439, 1511, 1796, 2111
CviQI	GTAC	6 cut(s)	354, 450, 894, 1017, 1125, 2105
EaeI	YGGCCR	2 cut(s)	378, 1044
Eam1104I	CTCTTC	4 cut(s)	589, 1029, 2034, 2064
EarI	CTCTTC	4 cut(s)	589, 1029, 2034, 2064
EciI	GGCGGA	1 cut(s)	2230
Eco130I	CCWWGG	2 cut(s)	1746, 2162
Eco24I	GRGCYC	3 cut(s)	1300, 1548, 1746
Eco31I	GGTCTC	1 cut(s)	101
Eco32I	GATATC	1 cut(s)	531
Eco47I	GGWCC	1 cut(s)	1894
Eco57I	CTGAAG	3 cut(s)	192, 1485, 1662
Eco81I	CCTNAGG	1 cut(s)	1452
Eco88I	CYCGRG	2 cut(s)	1412, 1910
EcoNI	CCTNNNNNAGG	1 cut(s)	955
EcoO109I	RGGNCCY	2 cut(s)	1296, 1894
EcoRI	GAATTC	3 cut(s)	276, 1151, 1946
EcoRII	CCWGG	2 cut(s)	281, 1307
EcoRV	GATATC	1 cut(s)	531
EcoT14I	CCWWGG	2 cut(s)	1746, 2162
EcoT22I	ATGCAT	1 cut(s)	786
EcoT38I	GRGCYC	3 cut(s)	1300, 1548, 1746
ErhI	CCWWGG	2 cut(s)	1746, 2162
FaeI	CATG	9 cut(s)	160, 325, 469, 661, 784, 1442, 1514, 1799, 2114
FatI	CATG	9 cut(s)	156, 321, 465, 657, 780, 1438, 1510, 1795, 2110
FauI	CCCGC	1 cut(s)	87
FauNDI	CATATG	1 cut(s)	933
FbaI	TGATCA	1 cut(s)	1993
FblI	GTMKAC	2 cut(s)	136, 611
Fnu4HI	GCNGC	7 cut(s)	5, 689, 846, 2052, 2210, 2253, 2256
FokI	GGATG	8 cut(s)	190, 713, 970, 1183, 1429, 2071, 2124, 2239
FriOI	GRGCYC	3 cut(s)	1300, 1548, 1746
Fsp4HI	GCNGC	7 cut(s)	5, 689, 846, 2052, 2210, 2253, 2256
FspBI	CTAG	4 cut(s)	359, 714, 1677, 1778
GlaI	GCGC	2 cut(s)	83, 1098
GluI	GCNGC	7 cut(s)	5, 689, 846, 2052, 2210, 2253, 2256
GsaI	CCCAGC	1 cut(s)	991
HaeII	RGCGCY	1 cut(s)	1100
HaeIII	GGCC	7 cut(s)	77, 380, 961, 1046, 1178, 1298, 2119
HapII	CCGG	2 cut(s)	8, 1763
HgaI	GACGC	2 cut(s)	24, 2107
HhaI	GCGC	2 cut(s)	84, 1099
Hin1I	GRCGYC	1 cut(s)	35
Hin1II	CATG	9 cut(s)	160, 325, 469, 661, 784, 1442, 1514, 1799, 2114
Hin6I	GCGC	2 cut(s)	82, 1097
HinP1I	GCGC	2 cut(s)	82, 1097
HinfI	GANTC	8 cut(s)	200, 221, 242, 500, 673, 721, 1087, 1555
HpaII	CCGG	2 cut(s)	8, 1763
HphI	GGTGA	4 cut(s)	940, 1324, 2178, 2245
Hpy166II	GTNNAC	6 cut(s)	137, 612, 1078, 1127, 1304, 1978
Hpy188I	TCNGA	6 cut(s)	259, 1056, 1380, 1543, 1857, 1956
Hpy8I	GTNNAC	6 cut(s)	137, 612, 1078, 1127, 1304, 1978
Hpy99I	CGWCG	5 cut(s)	16, 34, 59, 116, 1230
HpyAV	CCTTC	7 cut(s)	259, 418, 475, 958, 1509, 2088, 2217
HpyCH4III	ACNGT	4 cut(s)	148, 305, 609, 920
HpyCH4IV	ACGT	1 cut(s)	1277
HpyF10VI	GCNNNNNNNGC	4 cut(s)	314, 967, 2218, 2249
HpySE526I	ACGT	1 cut(s)	1277
Hsp92I	GRCGYC	1 cut(s)	35
Hsp92II	CATG	9 cut(s)	160, 325, 469, 661, 784, 1442, 1514, 1799, 2114
HspAI	GCGC	2 cut(s)	82, 1097
KpnI	GGTACC	1 cut(s)	2108
KroI	GCCGGC	1 cut(s)	7
KroNI	GCCGGC	1 cut(s)	9
Ksp22I	TGATCA	1 cut(s)	1993
LmnI	GCTCC	2 cut(s)	967, 1613
Lsp1109I	GCAGC	4 cut(s)	857, 2063, 2239, 2242
LweI	GCATC	1 cut(s)	2146
MaeI	CTAG	4 cut(s)	359, 714, 1677, 1778
MaeII	ACGT	1 cut(s)	1277
MaeIII	GTNAC	5 cut(s)	572, 906, 1278, 1441, 2184
MbiI	CCGCTC	1 cut(s)	999
MfeI	CAATTG	1 cut(s)	1658
MflI	RGATCY	4 cut(s)	487, 710, 1353, 1408
MhlI	GDGCHC	4 cut(s)	1300, 1548, 1746, 1980
MlsI	TGGCCA	2 cut(s)	380, 1046
MluNI	TGGCCA	2 cut(s)	380, 1046
MlyI	GAGTC	1 cut(s)	209
MmeI	TCCRAC	2 cut(s)	1298, 1798
Mox20I	TGGCCA	2 cut(s)	380, 1046
Mph1103I	ATGCAT	1 cut(s)	786
MreI	CGCCGGCG	1 cut(s)	7
MroNI	GCCGGC	1 cut(s)	7
MroXI	GAANNNNTTC	2 cut(s)	276, 881
MscI	TGGCCA	2 cut(s)	380, 1046
MseI	TTAA	7 cut(s)	684, 726, 801, 953, 1062, 1962, 2004
MslI	CAYNNNNRTG	1 cut(s)	656
Msp20I	TGGCCA	2 cut(s)	380, 1046
MspA1I	CMGCKG	2 cut(s)	212, 2209
MspI	CCGG	2 cut(s)	8, 1763
MspR9I	CCNGG	2 cut(s)	283, 1309
MunI	CAATTG	1 cut(s)	1658
Mva1269I	GAATGC	2 cut(s)	784, 1757
MvaI	CCWGG	2 cut(s)	283, 1309
MvnI	CGCG	1 cut(s)	82
MwoI	GCNNNNNNNGC	4 cut(s)	314, 967, 2218, 2249
NaeI	GCCGGC	1 cut(s)	9
NdeI	CATATG	1 cut(s)	933
NgoMIV	GCCGGC	1 cut(s)	7
NlaIII	CATG	9 cut(s)	160, 325, 469, 661, 784, 1442, 1514, 1799, 2114
NmeAIII	GCCGAG	2 cut(s)	53, 1048
NmuCI	GTSAC	4 cut(s)	572, 906, 1441, 2184
NsiI	ATGCAT	1 cut(s)	786
NspI	RCATGY	3 cut(s)	160, 784, 1442
PaeI	GCATGC	1 cut(s)	784
PagI	TCATGA	1 cut(s)	1510
PasI	CCCWGGG	2 cut(s)	282, 1308
PciI	ACATGT	1 cut(s)	1438
PcsI	WCGNNNNNNNCGW	1 cut(s)	111
PctI	GAATGC	2 cut(s)	784, 1757
PdiI	GCCGGC	1 cut(s)	9
PdmI	GAANNNNTTC	2 cut(s)	276, 881
PfeI	GAWTC	7 cut(s)	221, 242, 500, 673, 721, 1087, 1555
PflFI	GACNNNGTC	1 cut(s)	34
PkrI	GCNGC	7 cut(s)	6, 690, 847, 2053, 2211, 2254, 2257
PleI	GAGTC	1 cut(s)	208
PpsI	GAGTC	1 cut(s)	208
PpuMI	RGGWCCY	1 cut(s)	1894
PscI	ACATGT	1 cut(s)	1438
Psp1406I	AACGTT	1 cut(s)	1277
Psp5II	RGGWCCY	1 cut(s)	1894
Psp6I	CCWGG	2 cut(s)	281, 1307
PspFI	CCCAGC	1 cut(s)	987
PspGI	CCWGG	2 cut(s)	281, 1307
PspOMI	GGGCCC	1 cut(s)	1296
PspPI	GGNCC	5 cut(s)	76, 1176, 1296, 1297, 1894
PspPPI	RGGWCCY	1 cut(s)	1894
PstI	CTGCAG	1 cut(s)	388
PsuI	RGATCY	4 cut(s)	487, 710, 1353, 1408
PsyI	GACNNNGTC	1 cut(s)	34
PvuII	CAGCTG	1 cut(s)	212
RsaI	GTAC	6 cut(s)	355, 451, 895, 1018, 1126, 2106
RsaNI	GTAC	6 cut(s)	354, 450, 894, 1017, 1125, 2105
RseI	CAYNNNNRTG	1 cut(s)	656
SaqAI	TTAA	7 cut(s)	684, 726, 801, 953, 1062, 1962, 2004
SatI	GCNGC	7 cut(s)	5, 689, 846, 2052, 2210, 2253, 2256
Sau96I	GGNCC	5 cut(s)	76, 1176, 1296, 1297, 1894
ScaI	AGTACT	1 cut(s)	1018
SchI	GAGTC	1 cut(s)	209
ScrFI	CCNGG	2 cut(s)	283, 1309
SduI	GDGCHC	4 cut(s)	1300, 1548, 1746, 1980
SfaNI	GCATC	1 cut(s)	2146
SfcI	CTRYAG	3 cut(s)	225, 384, 2204
SgrAI	CRCCGGYG	1 cut(s)	7
SinI	GGWCC	1 cut(s)	1894
SmiMI	CAYNNNNRTG	1 cut(s)	656
SmlI	CTYRAG	1 cut(s)	197
SmoI	CTYRAG	1 cut(s)	197
SphI	GCATGC	1 cut(s)	784
SsiI	CCGC	6 cut(s)	5, 80, 688, 997, 2209, 2241
SspMI	CTAG	4 cut(s)	359, 714, 1677, 1778
StyD4I	CCNGG	2 cut(s)	281, 1307
StyI	CCWWGG	2 cut(s)	1746, 2162
TaaI	ACNGT	4 cut(s)	148, 305, 609, 920
TaiI	ACGT	1 cut(s)	1280
TaqI	TCGA	5 cut(s)	54, 90, 245, 1069, 1241
TaqII	GACCGA	1 cut(s)	125
TatI	WGTACW	4 cut(s)	353, 449, 1016, 1124
TauI	GCSGC	3 cut(s)	7, 691, 2212
TfiI	GAWTC	7 cut(s)	221, 242, 500, 673, 721, 1087, 1555
Tru1I	TTAA	7 cut(s)	684, 726, 801, 953, 1062, 1962, 2004
Tru9I	TTAA	7 cut(s)	684, 726, 801, 953, 1062, 1962, 2004
TscAI	CASTG	8 cut(s)	310, 388, 750, 781, 915, 1717, 1980, 2127
TseFI	GTSAC	4 cut(s)	572, 906, 1441, 2184
TseI	GCWGC	4 cut(s)	845, 2051, 2252, 2255
Tsp45I	GTSAC	4 cut(s)	572, 906, 1441, 2184
TspDTI	ATGAA	9 cut(s)	85, 132, 482, 632, 890, 950, 1311, 1491, 1499
TspGWI	ACGGA	4 cut(s)	29, 430, 1243, 1846
TspRI	CASTG	8 cut(s)	310, 388, 750, 781, 915, 1717, 1980, 2127
Tth111I	GACNNNGTC	1 cut(s)	34
VneI	GTGCAC	1 cut(s)	1976
VpaK11BI	GGWCC	1 cut(s)	1894
XagI	CCTNNNNNAGG	1 cut(s)	955
XapI	RAATTY	9 cut(s)	276, 974, 1050, 1151, 1361, 1457, 1631, 1946, 1988
XbaI	TCTAGA	2 cut(s)	358, 713
XceI	RCATGY	3 cut(s)	160, 784, 1442
XmiI	GTMKAC	2 cut(s)	136, 611
XmnI	GAANNNNTTC	2 cut(s)	276, 881
XspI	CTAG	4 cut(s)	359, 714, 1677, 1778
ZrmI	AGTACT	1 cut(s)	1018
Zsp2I	ATGCAT	1 cut(s)	786

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.