Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000750

Physical Location & Seq

Genomic Coordinates Forward (+)

31157539 .. 31162347

Gene Structure 4809 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1243900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGGCCGACCAAGAACCCAACGCGCCGGCTCCGACGGCGACGGGGATGATCGTCGACGATACGTACGAGTTCTCGGCTCCGCGCTTCTTCGACTTCATAAGGGACGAGAGCGAAGATGACCGGCGCAAGGCCGAGCTCTGGTTTGACTGCGCTCTCGCTTATGCTCCTTCACCTTTTATGCCTAAGATCAAGGCTGGTAGATCCTTTACGATCGAGAGCATGTGTGACTTTGGTGAAGCAGAGCAAATGCAGAATGTGAACTTGGAATCATCATCTGACTTAAAAGCTGATGATAAGTCTATCGAAACTAAGCCTAAATCAGACATCATGCCTGCTGTAGTTGCTGATAAGTCTATCGAAACTGAGCCTAAATCAGAGATCGTGCCTGCTGAAGTTGTCGAAACAATCCCCAGGGAAGCGAAAGAAGAAGGCAACATAAGCCAATTCATAGTCTCAGCTGATAAGAGTATTAGCCAAGTTGACAAAGACGAAGACAAATGCACTAGTGGAAATGGAAGTGTTTTGGGTACAGTATCTTCAAATAAGAAAGCTGTTTTGATCAGTGGTAAGGAGAATAGTAAACCTATCATTCAAGTTGAAATTGAAGCTTGCACTCCAAAACCACCCATGAGTTATTCCACGAAGCACCAGACTGCTAAAAAAATAGCAAGCATGGTCAGAAACCCATCAGCACTGAAGCAAAAAAGTCACTTACAATCATCCCAAACGAAAAACATTAAACCAAATAGTGTGAAAAGAGAAACACATGTGAAGAATGCAACTGGAACTCCACTTTTAGTTCAGGAAAATCAGGCGATAAAGCGGCAGAAATTGGACGAAGGAAGATCTAGACAGATTCTTAATCCCAAACCCCACGCATTGCCCCACAAGTCAAAACTGGGTCATACATGTTCTTCAGCTGCTTTGAAAAAAGAGGACCGAAAAGTTTATGTTAGGGAACCTGCATCACCATTTGTTTCGATGGCCGAAATGATGAAAAGGTTCCAATCAAGTACCAGAGACTTGTCATTGCCGCATACGAAACCCAAACTCACCTTGACTAGGCCTAAGGAGCCTGAATTTGAAACATCCCAGCGTGTTCGCTCAGTGAAAGTAAAGAGTACTGCTGAGCTTGAAGAAGAGATGATGGCTAAAATTCCAAAGTTTAAGGCTCGACCATTAAACAAAAAGATTCTAGAATCCCCATCATTGCCTGCAATGCCAAGAAGTACGCCCCAGCCACCGGAATTTCACGAATTCCATTTGGAGACAATGGCAAGGGCCAATCAGAATGCAGAAACATCCTCGATAGCTTCAACCGAAATGTCTCGTCAGAATAATCCATGGAAGCCTCATCATCTTACTGAACCTAAAACCCCTATGCTACACACATCATTGAGGGCTCGTCCACCCAGGGTGAAAAGTTCGTTGGAAATTGAACAGGAAGAACTCGAAAGAATCCCAAAATTCAAGGCCAAGCCTTTGAATAAGAAGATATTTGAAAGTAAAGGAGATATGGGGGTGTTCTGTAATGCAAAGAAACATGTGACCAAACCTCAGGAATTCCATTTTGCAACAAATGAAAGGATACCGCTGCAATCTTCGTCTGTTGTTGATCTACTTGATAAACTGTCGCTAAACTCAGAGCTTCACCATAATCCAGTTCCGAGAAACACAATGCCAAACCCATTCCATCTCCACACAGAGGAAAGAGGGGCCGAGAAAGAAAGGAAATTCATTACTGAAGTTTGGCACAAGCAATTGGAAGAAGAAAGAGCAAGGGTTCCAAAAGCTAATCCATATCCTTATACTACAGATTACCCTGTGATCCCACCCAAGCCAGAGCCCAAGCAATGCACAAAACCAGAACCTTTCCAACTAGAGAGTTTAGTGAGGCATGAGGAGGAATTGCAAAGGGAAATGGAAGAAAGACAGCGGAAGGAGAAAGAAGAAGCCCAGATAAGAGTGTTCAAGGCACAACCAATCTTGAAAGAGGACCCAATTCCTGTTCCCGAAAAAGAGCGCAAGCCTCTCACCCAAGTTCAGGAGTTCAATCTCCAAGTTGATCATCGAGCTGTTGATAGAGCCGAATTTGATCAAAAGGTTAGGGAGAAAGAGATGATGTACAAGAGATACAGAGAAGAGAGCGAGGCAGCGAGAATGGTCGAGGAAGAAAAGGCACTGAAACAACTCAGAAGGACATTGGTACCCCATGCAAGGCCGGTGCCCAACTTTGAGCATCCATTTTGTCCCCTAAAGTGCCCAAGGGAAGCAACAAAACCGAAATCACCACATCTACGTGTGCTCCAGCGAAAAGAAAGACGAAAAATGATGATCAACTCTGCAGTTTCCAGCGCTGCAGCACAGATGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032886 GO:0033043 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050789 GO:0050794 GO:0051128 GO:0051225 GO:0051493 GO:0051726 GO:0060236 GO:0065007 GO:0070013 GO:0070507 GO:0070925 GO:0071840 GO:0090224 GO:0090307 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2 TPX2_importin

Protein Analysis

792

Amino Acids

90.49

Weight (kDa)

9.2

Isoelectric Point (pI)

58.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2_importin	PF12214	352 - 442	9.4e-31	Cell cycle regulated microtubule associated protein
TPX2_importin	PF12214	453 - 535	4.8e-19	Cell cycle regulated microtubule associated protein
TPX2	PF06886	684 - 754	1e-21	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03780 AT1G03780 AT1G03780
fragaria_vesca	FvH4_2g11956 FvH4_5g28370 FvH4_5g28401 FvH4_5g28402 FvH4_5g28440 FvH4_5g28450 FvH4_5g28450 FvH4_5g28471 FvH4_5g28480 FvH4_5g28561 FvH4_6g25610 FvH4_6g25610 FvH4_6g25690 FvH4_6g25731 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_c15g00040 FvH4_c17g00020 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090
malus_domestica	MD09G1243900.v1.1 MD17G1235800.v1.1
prunus_persica	Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1
pyrus_communis	pycom17g23880
rosa_chinensis	RchiOBHm_Chr2g0127601
rosa_laevigata	RLG00000018552 RLG00000018951
rosa_multiflora	Rmu_ssc0000200.1_g000020
rosa_roxburghii	Rroxscaffold_2G00116560 Rroxscaffold_2G00123480
rosa_rugosa	Rorug02G0272600
rosa_samantha	Rh2AG324600 Rh2BG333900 Rh2CG312100 Rh2DG352100
rosa_wichuraiana	Rw2G026360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	970
Acc65I	GGTACC	1 cut(s)	2206
AccB1I	GGYRCC	2 cut(s)	2206, 2224
AccI	GTMKAC	1 cut(s)	54
AccII	CGCG	2 cut(s)	23, 82
AciI	CCGC	5 cut(s)	80, 823, 1034, 1592, 1936
AclWI	GGATC	2 cut(s)	195, 1822
AcoI	YGGCCR	2 cut(s)	3, 984
AcsI	RAATTY	8 cut(s)	1079, 1155, 1247, 1256, 1466, 1562, 1733, 2090
AcuI	CTGAAG	4 cut(s)	411, 716, 900, 1764
AfaI	GTAC	7 cut(s)	65, 529, 1015, 1123, 1231, 2126, 2208
AfeI	AGCGCT	1 cut(s)	2356
AfiI	CCNNNNNNNGG	7 cut(s)	127, 138, 1062, 1243, 1705, 2044, 2217
AflIII	ACRYGT	4 cut(s)	766, 908, 1543, 2299
AhlI	ACTAGT	1 cut(s)	503
AjnI	CCWGG	2 cut(s)	410, 1412
AjuI	GAANNNNNNNTTGG	4 cut(s)	245, 277, 1412, 1444
Alw21I	GWGCWC	2 cut(s)	138, 2307
Alw26I	GTCTC	4 cut(s)	457, 1014, 1262, 1332
AlwI	GGATC	2 cut(s)	195, 1822
Aor51HI	AGCGCT	1 cut(s)	2356
AoxI	GGCC	8 cut(s)	3, 129, 984, 1064, 1281, 1473, 1716, 2219
ApeKI	GCWGC	5 cut(s)	920, 1594, 2153, 2357, 2360
ApoI	RAATTY	8 cut(s)	1079, 1155, 1247, 1256, 1466, 1562, 1733, 2090
Asp700I	GAANNNNTTC	1 cut(s)	1001
Asp718I	GGTACC	1 cut(s)	2206
AspLEI	GCGC	6 cut(s)	25, 84, 126, 152, 2025, 2357
AspS9I	GGNCC	4 cut(s)	937, 1281, 1716, 1996
AsuHPI	GGTGA	8 cut(s)	162, 245, 960, 1045, 1429, 1643, 2026, 2280
AvaII	GGWCC	2 cut(s)	937, 1996
AxyI	CCTNAGG	2 cut(s)	1068, 1557
BaeGI	GKGCMC	2 cut(s)	2229, 2264
BaeI	ACNNNNGTAYC	2 cut(s)	2190, 2223
BanI	GGYRCC	2 cut(s)	2206, 2224
BanII	GRGCYC	3 cut(s)	138, 1405, 1848
BarI	GAAGNNNNNNTAC	2 cut(s)	520, 552
BbsI	GAAGAC	1 cut(s)	498
Bbv12I	GWGCWC	2 cut(s)	138, 2307
BbvI	GCAGC	5 cut(s)	907, 1581, 2165, 2344, 2372
BccI	CCATC	5 cut(s)	694, 976, 1141, 1213, 1701
BceAI	ACGGC	1 cut(s)	51
BciT130I	CCWGG	2 cut(s)	412, 1414
BciVI	GTATCC	1 cut(s)	1581
BclI	TGATCA	4 cut(s)	558, 2065, 2095, 2334
BcoDI	GTCTC	4 cut(s)	457, 1014, 1262, 1332
BcuI	ACTAGT	1 cut(s)	503
BfaI	CTAG	5 cut(s)	504, 849, 1062, 1196, 1880
BfmI	CTRYAG	4 cut(s)	336, 1812, 2343, 2358
BfoI	RGCGCY	1 cut(s)	2358
BfuAI	ACCTGC	1 cut(s)	970
BfuI	GTATCC	1 cut(s)	1581
BglII	AGATCT	1 cut(s)	845
BisI	GCNGC	7 cut(s)	824, 921, 1034, 1595, 2154, 2358, 2361
BlpI	GCTNAGC	1 cut(s)	1128
BlsI	GCNGC	7 cut(s)	825, 922, 1035, 1596, 2155, 2359, 2362
BmcAI	AGTACT	1 cut(s)	1123
Bme1390I	CCNGG	2 cut(s)	412, 1414
Bme18I	GGWCC	2 cut(s)	937, 1996
BmgT120I	GGNCC	4 cut(s)	937, 1281, 1716, 1996
BmrFI	CCNGG	2 cut(s)	412, 1414
BmrI	ACTGGG	1 cut(s)	908
BmsI	GCATC	3 cut(s)	974, 2248, 2358
BmuI	ACTGGG	1 cut(s)	908
BpiI	GAAGAC	1 cut(s)	498
BpmI	CTGGAG	1 cut(s)	2291
Bpu1102I	GCTNAGC	1 cut(s)	1128
BsaAI	YACGTR	2 cut(s)	63, 2300
BsaJI	CCNNGG	6 cut(s)	410, 411, 1343, 1412, 1413, 2264
BsaWI	WCCGGW	1 cut(s)	1243
BsaXI	ACNNNNNCTCC	4 cut(s)	772, 802, 2289, 2319
Bsc4I	CCNNNNNNNGG	7 cut(s)	127, 138, 1062, 1243, 1705, 2044, 2217
Bse118I	RCCGGY	3 cut(s)	25, 120, 2221
Bse1I	ACTGG	3 cut(s)	787, 903, 1661
Bse21I	CCTNAGG	2 cut(s)	1068, 1557
Bse3DI	GCAATG	5 cut(s)	878, 1028, 1208, 1224, 1859
BseBI	CCWGG	2 cut(s)	412, 1414
BseDI	CCNNGG	6 cut(s)	410, 411, 1343, 1412, 1413, 2264
BseGI	GGATG	5 cut(s)	51, 719, 1088, 1301, 2239
BseLI	CCNNNNNNNGG	7 cut(s)	127, 138, 1062, 1243, 1705, 2044, 2217
BseMI	GCAATG	5 cut(s)	878, 1028, 1208, 1224, 1859
BseMII	CTCAG	7 cut(s)	354, 468, 1119, 1119, 1571, 1656, 2206
BseNI	ACTGG	3 cut(s)	787, 903, 1661
BseRI	GAGGAG	1 cut(s)	1916
BseSI	GKGCMC	2 cut(s)	2229, 2264
BseXI	GCAGC	5 cut(s)	907, 1581, 2165, 2344, 2372
BseYI	CCCAGC	2 cut(s)	1092, 1236
Bsh1236I	CGCG	2 cut(s)	23, 82
Bsh1285I	CGRYCG	1 cut(s)	213
BshFI	GGCC	8 cut(s)	5, 131, 986, 1066, 1283, 1475, 1718, 2221
BshNI	GGYRCC	2 cut(s)	2206, 2224
BsiEI	CGRYCG	1 cut(s)	213
BsiHKAI	GWGCWC	2 cut(s)	138, 2307
BsiSI	CCGG	4 cut(s)	26, 121, 1244, 2222
BsiWI	CGTACG	1 cut(s)	63
BslFI	GGGAC	2 cut(s)	116, 2235
BslI	CCNNNNNNNGG	7 cut(s)	127, 138, 1062, 1243, 1705, 2044, 2217
BsmAI	GTCTC	4 cut(s)	457, 1014, 1262, 1332
BsmFI	GGGAC	2 cut(s)	116, 2235
BsmI	GAATGC	2 cut(s)	781, 1297
BsnI	GGCC	8 cut(s)	5, 131, 986, 1066, 1283, 1475, 1718, 2221
Bsp1286I	GDGCHC	6 cut(s)	138, 1405, 1848, 2229, 2264, 2307
Bsp1407I	TGTACA	1 cut(s)	2124
Bsp1720I	GCTNAGC	1 cut(s)	1128
Bsp19I	CCATGG	1 cut(s)	1343
BspACI	CCGC	5 cut(s)	80, 823, 1034, 1592, 1936
BspANI	GGCC	8 cut(s)	5, 131, 986, 1066, 1283, 1475, 1718, 2221
BspCNI	CTCAG	7 cut(s)	355, 467, 1118, 1120, 1570, 1655, 2205
BspFNI	CGCG	2 cut(s)	23, 82
BspMAI	CTGCAG	2 cut(s)	2347, 2362
BspMI	ACCTGC	1 cut(s)	970
BspPI	GGATC	2 cut(s)	195, 1822
BspT107I	GGYRCC	2 cut(s)	2206, 2224
BsrDI	GCAATG	5 cut(s)	878, 1028, 1208, 1224, 1859
BsrFI	RCCGGY	3 cut(s)	25, 120, 2221
BsrGI	TGTACA	1 cut(s)	2124
BsrI	ACTGG	3 cut(s)	787, 903, 1661
BssAI	RCCGGY	3 cut(s)	25, 120, 2221
BssECI	CCNNGG	6 cut(s)	410, 411, 1343, 1412, 1413, 2264
BssT1I	CCWWGG	2 cut(s)	1343, 2264
Bst2UI	CCWGG	2 cut(s)	412, 1414
Bst4CI	ACNGT	2 cut(s)	532, 1632
Bst6I	CTCTTC	2 cut(s)	1134, 2136
BstAUI	TGTACA	1 cut(s)	2124
BstBAI	YACGTR	2 cut(s)	63, 2300
BstC8I	GCNNGC	7 cut(s)	27, 333, 387, 610, 670, 1215, 2027
BstDSI	CCRYGG	1 cut(s)	1343
BstENI	CCTNNNNNAGG	1 cut(s)	1060
BstF5I	GGATG	5 cut(s)	51, 719, 1088, 1301, 2239
BstFNI	CGCG	2 cut(s)	23, 82
BstH2I	RGCGCY	1 cut(s)	2358
BstHHI	GCGC	6 cut(s)	25, 84, 126, 152, 2025, 2357
BstMAI	GTCTC	4 cut(s)	457, 1014, 1262, 1332
BstMCI	CGRYCG	1 cut(s)	213
BstMWI	GCNNNNNNNGC	5 cut(s)	35, 341, 438, 1072, 1219
BstNI	CCWGG	2 cut(s)	412, 1414
BstNSI	RCATGY	4 cut(s)	223, 770, 912, 1547
BstSCI	CCNGG	2 cut(s)	410, 1412
BstSFI	CTRYAG	4 cut(s)	336, 1812, 2343, 2358
BstSLI	GKGCMC	2 cut(s)	2229, 2264
BstSNI	TACGTA	1 cut(s)	63
BstUI	CGCG	2 cut(s)	23, 82
BstV1I	GCAGC	5 cut(s)	907, 1581, 2165, 2344, 2372
BstV2I	GAAGAC	1 cut(s)	498
BstX2I	RGATCY	2 cut(s)	200, 845
BstYI	RGATCY	2 cut(s)	200, 845
Bsu36I	CCTNAGG	2 cut(s)	1068, 1557
BsuI	GTATCC	1 cut(s)	1581
BsuRI	GGCC	8 cut(s)	5, 131, 986, 1066, 1283, 1475, 1718, 2221
BtgI	CCRYGG	1 cut(s)	1343
BtsCI	GGATG	5 cut(s)	51, 719, 1088, 1301, 2239
BtsIMutI	CAGTG	4 cut(s)	568, 692, 1113, 2180
BveI	ACCTGC	1 cut(s)	970
Cac8I	GCNNGC	7 cut(s)	27, 333, 387, 610, 670, 1215, 2027
CfoI	GCGC	6 cut(s)	25, 84, 126, 152, 2025, 2357
Cfr10I	RCCGGY	3 cut(s)	25, 120, 2221
Cfr13I	GGNCC	4 cut(s)	937, 1281, 1716, 1996
Csp6I	GTAC	7 cut(s)	64, 528, 1014, 1122, 1230, 2125, 2207
CviQI	GTAC	7 cut(s)	64, 528, 1014, 1122, 1230, 2125, 2207
EaeI	YGGCCR	2 cut(s)	3, 984
Eam1104I	CTCTTC	2 cut(s)	1134, 2136
EarI	CTCTTC	2 cut(s)	1134, 2136
Ecl136II	GAGCTC	1 cut(s)	136
Eco105I	TACGTA	1 cut(s)	63
Eco130I	CCWWGG	2 cut(s)	1343, 2264
Eco147I	AGGCCT	1 cut(s)	1066
Eco24I	GRGCYC	3 cut(s)	138, 1405, 1848
Eco47I	GGWCC	2 cut(s)	937, 1996
Eco47III	AGCGCT	1 cut(s)	2356
Eco53kI	GAGCTC	1 cut(s)	136
Eco57I	CTGAAG	4 cut(s)	411, 716, 900, 1764
Eco81I	CCTNAGG	2 cut(s)	1068, 1557
EcoICRI	GAGCTC	1 cut(s)	136
EcoNI	CCTNNNNNAGG	1 cut(s)	1060
EcoO109I	RGGNCCY	1 cut(s)	1996
EcoRI	GAATTC	2 cut(s)	1256, 1562
EcoRII	CCWGG	2 cut(s)	410, 1412
EcoT14I	CCWWGG	2 cut(s)	1343, 2264
EcoT38I	GRGCYC	3 cut(s)	138, 1405, 1848
ErhI	CCWWGG	2 cut(s)	1343, 2264
FaqI	GGGAC	2 cut(s)	116, 2235
FbaI	TGATCA	4 cut(s)	558, 2065, 2095, 2334
FblI	GTMKAC	1 cut(s)	54
Fnu4HI	GCNGC	7 cut(s)	824, 921, 1034, 1595, 2154, 2358, 2361
FokI	GGATG	5 cut(s)	58, 706, 1075, 1288, 2226
FriOI	GRGCYC	3 cut(s)	138, 1405, 1848
Fsp4HI	GCNGC	7 cut(s)	824, 921, 1034, 1595, 2154, 2358, 2361
FspBI	CTAG	5 cut(s)	504, 849, 1062, 1196, 1880
GlaI	GCGC	6 cut(s)	24, 83, 125, 151, 2024, 2356
GluI	GCNGC	7 cut(s)	824, 921, 1034, 1595, 2154, 2358, 2361
GsaI	CCCAGC	2 cut(s)	1096, 1240
GsuI	CTGGAG	1 cut(s)	2291
HaeII	RGCGCY	1 cut(s)	2358
HaeIII	GGCC	8 cut(s)	5, 131, 986, 1066, 1283, 1475, 1718, 2221
HapII	CCGG	4 cut(s)	26, 121, 1244, 2222
HhaI	GCGC	6 cut(s)	25, 84, 126, 152, 2025, 2357
Hin6I	GCGC	6 cut(s)	23, 82, 124, 150, 2023, 2355
HinP1I	GCGC	6 cut(s)	23, 82, 124, 150, 2023, 2355
HincII	GTYRAC	2 cut(s)	55, 481
HindII	GTYRAC	2 cut(s)	55, 481
HindIII	AAGCTT	1 cut(s)	606
HinfI	GANTC	5 cut(s)	266, 856, 1192, 1199, 1458
HpaII	CCGG	4 cut(s)	26, 121, 1244, 2222
HphI	GGTGA	8 cut(s)	162, 245, 960, 1045, 1429, 1643, 2026, 2280
Hpy166II	GTNNAC	5 cut(s)	55, 259, 481, 581, 1409
Hpy188III	TCNNGA	9 cut(s)	214, 803, 849, 1196, 1253, 1559, 1987, 2012, 2045
Hpy8I	GTNNAC	5 cut(s)	55, 259, 481, 581, 1409
Hpy99I	CGWCG	4 cut(s)	37, 43, 56, 59
HpyAV	CCTTC	5 cut(s)	177, 422, 833, 1933, 2190
HpyCH4III	ACNGT	2 cut(s)	532, 1632
HpyCH4IV	ACGT	2 cut(s)	62, 2299
HpyF10VI	GCNNNNNNNGC	5 cut(s)	35, 341, 438, 1072, 1219
HpySE526I	ACGT	2 cut(s)	62, 2299
HspAI	GCGC	6 cut(s)	23, 82, 124, 150, 2023, 2355
KpnI	GGTACC	1 cut(s)	2210
KroI	GCCGGC	1 cut(s)	25
KroNI	GCCGGC	1 cut(s)	27
Ksp22I	TGATCA	4 cut(s)	558, 2065, 2095, 2334
LmnI	GCTCC	5 cut(s)	34, 82, 169, 1072, 2310
Lsp1109I	GCAGC	5 cut(s)	907, 1581, 2165, 2344, 2372
LweI	GCATC	3 cut(s)	974, 2248, 2358
MaeI	CTAG	5 cut(s)	504, 849, 1062, 1196, 1880
MaeII	ACGT	2 cut(s)	62, 2299
MaeIII	GTNAC	3 cut(s)	224, 707, 1546
MfeI	CAATTG	1 cut(s)	1760
MflI	RGATCY	2 cut(s)	200, 845
MhlI	GDGCHC	6 cut(s)	138, 1405, 1848, 2229, 2264, 2307
MmeI	TCCRAC	3 cut(s)	56, 1410, 1900
MroNI	GCCGGC	1 cut(s)	25
MroXI	GAANNNNTTC	1 cut(s)	1001
MseI	TTAA	5 cut(s)	281, 738, 861, 1167, 1181
MslI	CAYNNNNRTG	1 cut(s)	2298
MspA1I	CMGCKG	4 cut(s)	458, 920, 1594, 1936
MspI	CCGG	4 cut(s)	26, 121, 1244, 2222
MspR9I	CCNGG	2 cut(s)	412, 1414
MunI	CAATTG	1 cut(s)	1760
Mva1269I	GAATGC	2 cut(s)	781, 1297
MvaI	CCWGG	2 cut(s)	412, 1414
MvnI	CGCG	2 cut(s)	23, 82
MwoI	GCNNNNNNNGC	5 cut(s)	35, 341, 438, 1072, 1219
NaeI	GCCGGC	1 cut(s)	27
NcoI	CCATGG	1 cut(s)	1343
NgoMIV	GCCGGC	1 cut(s)	25
NmeAIII	GCCGAG	3 cut(s)	53, 157, 1744
NmuCI	GTSAC	3 cut(s)	224, 707, 1546
NspI	RCATGY	4 cut(s)	223, 770, 912, 1547
PasI	CCCWGGG	2 cut(s)	411, 1413
PceI	AGGCCT	1 cut(s)	1066
PciI	ACATGT	3 cut(s)	766, 908, 1543
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PctI	GAATGC	2 cut(s)	781, 1297
PdiI	GCCGGC	1 cut(s)	27
PdmI	GAANNNNTTC	1 cut(s)	1001
PfeI	GAWTC	5 cut(s)	266, 856, 1192, 1199, 1458
Pfl23II	CGTACG	1 cut(s)	63
PkrI	GCNGC	7 cut(s)	825, 922, 1035, 1596, 2155, 2359, 2362
Ple19I	CGATCG	1 cut(s)	213
Ppu21I	YACGTR	2 cut(s)	63, 2300
PpuMI	RGGWCCY	1 cut(s)	1996
PscI	ACATGT	3 cut(s)	766, 908, 1543
Psp124BI	GAGCTC	1 cut(s)	138
Psp5II	RGGWCCY	1 cut(s)	1996
Psp6I	CCWGG	2 cut(s)	410, 1412
PspFI	CCCAGC	2 cut(s)	1092, 1236
PspGI	CCWGG	2 cut(s)	410, 1412
PspLI	CGTACG	1 cut(s)	63
PspPI	GGNCC	4 cut(s)	937, 1281, 1716, 1996
PspPPI	RGGWCCY	1 cut(s)	1996
PstI	CTGCAG	2 cut(s)	2347, 2362
PsuI	RGATCY	2 cut(s)	200, 845
PvuI	CGATCG	1 cut(s)	213
PvuII	CAGCTG	2 cut(s)	458, 920
RsaI	GTAC	7 cut(s)	65, 529, 1015, 1123, 1231, 2126, 2208
RsaNI	GTAC	7 cut(s)	64, 528, 1014, 1122, 1230, 2125, 2207
RseI	CAYNNNNRTG	1 cut(s)	2298
SacI	GAGCTC	1 cut(s)	138
SalI	GTCGAC	1 cut(s)	53
SaqAI	TTAA	5 cut(s)	281, 738, 861, 1167, 1181
SatI	GCNGC	7 cut(s)	824, 921, 1034, 1595, 2154, 2358, 2361
Sau96I	GGNCC	4 cut(s)	937, 1281, 1716, 1996
ScaI	AGTACT	1 cut(s)	1123
ScrFI	CCNGG	2 cut(s)	412, 1414
SduI	GDGCHC	6 cut(s)	138, 1405, 1848, 2229, 2264, 2307
SfaNI	GCATC	3 cut(s)	974, 2248, 2358
SfcI	CTRYAG	4 cut(s)	336, 1812, 2343, 2358
SgrDI	CGTCGACG	1 cut(s)	53
SinI	GGWCC	2 cut(s)	937, 1996
SmiMI	CAYNNNNRTG	1 cut(s)	2298
SnaBI	TACGTA	1 cut(s)	63
SpeI	ACTAGT	1 cut(s)	503
SseBI	AGGCCT	1 cut(s)	1066
SsiI	CCGC	5 cut(s)	80, 823, 1034, 1592, 1936
SspMI	CTAG	5 cut(s)	504, 849, 1062, 1196, 1880
SstI	GAGCTC	1 cut(s)	138
StuI	AGGCCT	1 cut(s)	1066
StyD4I	CCNGG	2 cut(s)	410, 1412
StyI	CCWWGG	2 cut(s)	1343, 2264
TaaI	ACNGT	2 cut(s)	532, 1632
TaiI	ACGT	2 cut(s)	65, 2302
TaqII	GACCGA	1 cut(s)	954
TatI	WGTACW	2 cut(s)	1121, 2124
TauI	GCSGC	2 cut(s)	826, 1036
TfiI	GAWTC	5 cut(s)	266, 856, 1192, 1199, 1458
Tru1I	TTAA	5 cut(s)	281, 738, 861, 1167, 1181
Tru9I	TTAA	5 cut(s)	281, 738, 861, 1167, 1181
TscAI	CASTG	4 cut(s)	568, 699, 1113, 2187
TseFI	GTSAC	3 cut(s)	224, 707, 1546
TseI	GCWGC	5 cut(s)	920, 1594, 2153, 2357, 2360
Tsp45I	GTSAC	3 cut(s)	224, 707, 1546
TspDTI	ATGAA	5 cut(s)	85, 436, 1010, 1596, 1726
TspRI	CASTG	4 cut(s)	568, 699, 1113, 2187
VpaK11BI	GGWCC	2 cut(s)	937, 1996
XagI	CCTNNNNNAGG	1 cut(s)	1060
XapI	RAATTY	8 cut(s)	1079, 1155, 1247, 1256, 1466, 1562, 1733, 2090
XbaI	TCTAGA	2 cut(s)	848, 1195
XceI	RCATGY	4 cut(s)	223, 770, 912, 1547
XmiI	GTMKAC	1 cut(s)	54
XmnI	GAANNNNTTC	1 cut(s)	1001
XspI	CTAG	5 cut(s)	504, 849, 1062, 1196, 1880
ZrmI	AGTACT	1 cut(s)	1123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.