Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0000750

Physical Location & Seq

Genomic Coordinates Forward (+)

43790243 .. 43795418

Gene Structure 5176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00116560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGCCGCCGGCGACGGAGGAACCTAAGACGACGGCGTCCTCGACGACGACGACGATGATGATCGACGAGATTTATGAGTTCTCGGCTCCGCGCTTCTTCGATTTCATCAAGGACGAGACCGACGACGACAAGATGAAGGCCGAGCTCTGGTTCGACTCCGCCACAGCCTACGCCCCTTCCCCTTGCATGCCTAGAATCAAGACTGGTAGATCCTTTACAGTAGAGAGCATGTGCGACTTTACTGAAGCAGAAAATATGCAGAAGGTGAACCTTGAATCATCCTCAGCTGATGATGATTCTACAGAAACCAAGACTGAGTCGAAGATCACTCCTGAATTGAAGGAAGAAACAATTCCCAGGGAGGCTAAAGAAGAAAACAGTGCAAGACAAGCCAATATGATTTCTGCTGATAAAGTAGAGGAAAAATGTACTATAGATGATGAAAGTGTTTTGGCCACTGCAGTATCTTTGAATAAGAGAACTGATATGACAAATGAGAAGGAGAATGGTAATTCCGTAGCTTGTACTCCAAAACCACCCATGAATTATTCCAAGAAGGGAGATCTTCTGAGTGATTCTAAAAAGCACCAGACAGCTAAAAAGATATCAAGTATGGTTAGAAACCCATCAGGGCTAAGGGGAAAAAGTCACTTGCACTCTTCACAAACTAAGAATGTCAAACCGTATACTATGAAAAGAGAAGAAAATGTGAAAAATATAACTGGCACACCCATGTTAGCTCAGGAGAATCAGGCAATTAAGCGGCAGAAGTTGGATGGAGGGAGATCTAGACAGATTCTTAATCCCAAACCCCAAACATTGCCCCACAAGTCAAAATTGGGTTATACCAGTGGCATGCATTCTTCAACTGCTGTTAAAACTCAAAAAGAGGACAGAAAGATGTATGTTCGGGAACCAGCAGCACCCTTTGTTTCAATGGCAGAAATGATGAACAGGTTCCAATCAAGTACCAGAGACTTGTCACTGCCACACAGTTCTCTTTCACATATGAAGCCCAAACTCACCTTAACAAGGCCGAAGGAGCCTGAATTTGAAACATCCCAGCGTGTCCGCTCAGTAAGAGTAAAGAGTACTGCCGAGCTTGAAGAAGAGATGATGGCCAAAATTCCAAAGTTTAAGGCTCGACCTGTAAACAAAAAGATTCTTGAAGCGCCATCATTACCTGCAAAGCCAAGAAGTACACCACAGCCACCTGAGTTTCAGGAATTCCATTTAGAGACTATGGCAAGGGCCAATCAAAATGCAGAAACATCCTCAGTAGCTTCAACTGATGTATCACGACAGAACAAACAATCGAAGCCCCATCATCTTACTGAACCGAAAACTCCAACGTTACATACATTATTGAGGGCCCGTCCACCCAGGGTGAAAACGTCATTGGAAATTGAGCAGGAAGAACTTGAAAAGATCCCAAAATTCAAAGCGAAGCCTCTGAATAAGAAGATATTTGAAAGTAAAGGAGATCTCGGGATGTTCTGTAATGCCAAGAAACATGTGACTAAACCTCAGGAATTTCACTTTGCAACAAATGAAAGAATACCACCACCACCACCACCTTCAGCCATTCATGATCTATTTGATAAGTTGTCTTTGAACTCAGAGCCCCACCAGAATCCAATTCCAAGAAACACAATGCCAAATCCATTCCATCTCCATACAGAGGAAAGAGGAGCTGAGAAAGAAAAGAAATTTTTTACTGAAGTTATGCAGAAGCAATTGGAAGAAGAAAGAGCTAGGGTTCCCAAAGCTAATCCATATCCTTATACCACTGATTACCCTGTGATCCCACCAAAGCCAGAGCCCAAAGAATGCACAAAACCAGAACCTTTCCAACTAGAGAGCTTAGCGAGGCATGAGGAGGAAATGCAAAGAGAAATGGAAGAAAGACGGAGGATAGAGATGGAAGAAGCTCAGATGAGGGTTTTCAAGGCACAACCAATCTTGAAAGAGGACCCAATTCCAGTTCCCGAGAGAGTTCGCAAGCCTCTAACAGAAGTTCAAGAATTCAATCTGAATGTTAATAATCGGGCAGTGCACAGAGCAGAATTTGATCACAAGGTTAAGGAGAAAGAGATAATATATAAGAGATATAGAGAAGAGAGTGAAGCAGCAAGGATGTTAGAGGAAGAGAAAGCATTGAAACAACTGAGAAGGACGCTGGTACCCCATGCAAGGCCAGTGCCTAATTTTGAGCATCCATTCTGCCCCCAGAAGTGTCCAAGGGAAACAACAAAAGCAAAGTCACCAAATCTGCGAGTTCTACAGCGGCAAGAAAGGCGAAGGATGATGGTCAAATCCGCCACTTCCAGTGCTGCTGCCCAGATGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0007346 GO:0008017 GO:0008092 GO:0008150 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0010564 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022607 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032886 GO:0033043 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050789 GO:0050794 GO:0051128 GO:0051225 GO:0051493 GO:0051726 GO:0060236 GO:0065007 GO:0070013 GO:0070507 GO:0070925 GO:0071840 GO:0090224 GO:0090307 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140014 GO:1902850 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPX2 TPX2_importin

Protein Analysis

783

Amino Acids

89.89

Weight (kDa)

9.25

Isoelectric Point (pI)

60.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPX2_importin	PF12214	342 - 438	5.3e-32	Cell cycle regulated microtubule associated protein
TPX2_importin	PF12214	443 - 520	8.7e-18	Cell cycle regulated microtubule associated protein
TPX2	PF06886	676 - 751	8.4e-21	Targeting protein for Xklp2 (TPX2) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000750)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03780 AT1G03780 AT1G03780
fragaria_vesca	FvH4_2g11956 FvH4_5g28370 FvH4_5g28401 FvH4_5g28402 FvH4_5g28440 FvH4_5g28450 FvH4_5g28450 FvH4_5g28471 FvH4_5g28480 FvH4_5g28561 FvH4_6g25610 FvH4_6g25610 FvH4_6g25690 FvH4_6g25731 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_6g26550 FvH4_c15g00040 FvH4_c17g00020 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090 FvH4_c17g00090
malus_domestica	MD09G1243900.v1.1 MD17G1235800.v1.1
prunus_persica	Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1 Prupe.3G140300_v2.0.a1
pyrus_communis	pycom17g23880
rosa_chinensis	RchiOBHm_Chr2g0127601
rosa_laevigata	RLG00000018552 RLG00000018951
rosa_multiflora	Rmu_ssc0000200.1_g000020
rosa_roxburghii	Rroxscaffold_2G00116560 Rroxscaffold_2G00123480
rosa_rugosa	Rorug02G0272600
rosa_samantha	Rh2AG324600 Rh2BG333900 Rh2CG312100 Rh2DG352100
rosa_wichuraiana	Rw2G026360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	34
Acc36I	ACCTGC	1 cut(s)	1192
Acc65I	GGTACC	1 cut(s)	2182
AccB1I	GGYRCC	1 cut(s)	2182
AccBSI	CCGCTC	1 cut(s)	1074
AccI	GTMKAC	1 cut(s)	686
AccII	CGCG	1 cut(s)	91
AciI	CCGC	7 cut(s)	5, 89, 159, 763, 1072, 2287, 2319
AclI	AACGTT	1 cut(s)	1352
AclWI	GGATC	3 cut(s)	204, 1423, 1798
AcoI	YGGCCR	2 cut(s)	453, 1119
AcsI	RAATTY	8 cut(s)	1049, 1125, 1226, 1436, 1532, 1709, 2024, 2066
AcuI	CTGAAG	3 cut(s)	264, 1563, 1740
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	6 cut(s)	430, 526, 970, 1093, 1201, 2184
AfiI	CCNNNNNNNGG	4 cut(s)	147, 1032, 1681, 2193
AflIII	ACRYGT	1 cut(s)	1513
AjnI	CCWGG	2 cut(s)	356, 1382
AjuI	GAANNNNNNNTTGG	2 cut(s)	1382, 1414
Alw21I	GWGCWC	2 cut(s)	147, 2058
Alw26I	GTCTC	3 cut(s)	110, 969, 1232
Alw44I	GTGCAC	1 cut(s)	2054
AlwI	GGATC	3 cut(s)	204, 1423, 1798
Ama87I	CYCGRG	2 cut(s)	1487, 1988
AoxI	GGCC	7 cut(s)	138, 453, 1034, 1119, 1251, 1371, 2195
ApaI	GGGCCC	1 cut(s)	1375
ApaLI	GTGCAC	1 cut(s)	2054
ApeKI	GCWGC	4 cut(s)	920, 2129, 2333, 2336
ApoI	RAATTY	8 cut(s)	1049, 1125, 1226, 1436, 1532, 1709, 2024, 2066
ArsI	GACNNNNNNTTYG	2 cut(s)	2246, 2278
Asp700I	GAANNNNTTC	2 cut(s)	351, 956
Asp718I	GGTACC	1 cut(s)	2182
AspLEI	GCGC	2 cut(s)	93, 1174
AspS9I	GGNCC	4 cut(s)	1251, 1371, 1372, 1972
AsuHPI	GGTGA	4 cut(s)	277, 1015, 1399, 2256
AvaI	CYCGRG	2 cut(s)	1487, 1988
AvaII	GGWCC	1 cut(s)	1972
AxyI	CCTNAGG	1 cut(s)	1527
BaeGI	GKGCMC	2 cut(s)	1375, 2058
BaeI	ACNNNNGTAYC	6 cut(s)	447, 480, 1278, 1311, 2166, 2199
BalI	TGGCCA	2 cut(s)	455, 1121
BanI	GGYRCC	1 cut(s)	2182
BanII	GRGCYC	4 cut(s)	147, 1375, 1626, 1824
Bbv12I	GWGCWC	2 cut(s)	147, 2058
BbvCI	CCTCAGC	1 cut(s)	283
BbvI	GCAGC	4 cut(s)	932, 2141, 2320, 2323
BccI	CCATC	8 cut(s)	634, 770, 1111, 1183, 1332, 1677, 1915, 2302
BceAI	ACGGC	1 cut(s)	48
BciT130I	CCWGG	2 cut(s)	358, 1384
BclI	TGATCA	1 cut(s)	2071
BcoDI	GTCTC	3 cut(s)	110, 969, 1232
BfaI	CTAG	4 cut(s)	192, 789, 1755, 1856
BfmI	CTRYAG	4 cut(s)	300, 432, 459, 2282
BfoI	RGCGCY	1 cut(s)	1175
BfuAI	ACCTGC	1 cut(s)	1192
BglII	AGATCT	3 cut(s)	562, 785, 1483
BisI	GCNGC	7 cut(s)	5, 764, 921, 2130, 2288, 2334, 2337
BlpI	GCTNAGC	1 cut(s)	1864
BlsI	GCNGC	7 cut(s)	6, 765, 922, 2131, 2289, 2335, 2338
BmcAI	AGTACT	1 cut(s)	1093
Bme1390I	CCNGG	2 cut(s)	358, 1384
Bme18I	GGWCC	1 cut(s)	1972
BmeT110I	CYCGRG	2 cut(s)	1487, 1988
BmgT120I	GGNCC	4 cut(s)	1251, 1371, 1372, 1972
BmiI	GGNNCC	9 cut(s)	21, 87, 915, 959, 1044, 1373, 1761, 1974, 2184
BmrFI	CCNGG	2 cut(s)	358, 1384
BmsI	GCATC	1 cut(s)	2224
Bpu10I	CCTNAGC	3 cut(s)	283, 635, 741
Bpu1102I	GCTNAGC	1 cut(s)	1864
BsaBI	GATNNNNATC	1 cut(s)	59
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	1 cut(s)	110
BsaJI	CCNNGG	5 cut(s)	356, 357, 1382, 1383, 2240
Bsc4I	CCNNNNNNNGG	4 cut(s)	147, 1032, 1681, 2193
Bse118I	RCCGGY	1 cut(s)	7
Bse1I	ACTGG	6 cut(s)	208, 727, 849, 1982, 2198, 2328
Bse21I	CCTNAGG	1 cut(s)	1527
Bse3DI	GCAATG	1 cut(s)	818
Bse8I	GATNNNNATC	1 cut(s)	59
BseBI	CCWGG	2 cut(s)	358, 1384
BseDI	CCNNGG	5 cut(s)	356, 357, 1382, 1383, 2240
BseGI	GGATG	8 cut(s)	278, 781, 1058, 1271, 1497, 2142, 2215, 2310
BseJI	GATNNNNATC	1 cut(s)	59
BseLI	CCNNNNNNNGG	4 cut(s)	147, 1032, 1681, 2193
BseMI	GCAATG	1 cut(s)	818
BseNI	ACTGG	6 cut(s)	208, 727, 849, 1982, 2198, 2328
BseRI	GAGGAG	2 cut(s)	1704, 1892
BseSI	GKGCMC	2 cut(s)	1375, 2058
BseXI	GCAGC	4 cut(s)	932, 2141, 2320, 2323
BseYI	CCCAGC	1 cut(s)	1062
Bsh1236I	CGCG	1 cut(s)	91
BshFI	GGCC	7 cut(s)	140, 455, 1036, 1121, 1253, 1373, 2197
BshNI	GGYRCC	1 cut(s)	2182
BsiHKAI	GWGCWC	2 cut(s)	147, 2058
BsiHKCI	CYCGRG	2 cut(s)	1487, 1988
BsiSI	CCGG	1 cut(s)	8
BslI	CCNNNNNNNGG	4 cut(s)	147, 1032, 1681, 2193
BsmAI	GTCTC	3 cut(s)	110, 969, 1232
BsmI	GAATGC	2 cut(s)	859, 1835
BsnI	GGCC	7 cut(s)	140, 455, 1036, 1121, 1253, 1373, 2197
Bso31I	GGTCTC	1 cut(s)	110
BsoBI	CYCGRG	2 cut(s)	1487, 1988
Bsp120I	GGGCCC	1 cut(s)	1371
Bsp1286I	GDGCHC	5 cut(s)	147, 1375, 1626, 1824, 2058
Bsp1720I	GCTNAGC	1 cut(s)	1864
BspACI	CCGC	7 cut(s)	5, 89, 159, 763, 1072, 2287, 2319
BspANI	GGCC	7 cut(s)	140, 455, 1036, 1121, 1253, 1373, 2197
BspFNI	CGCG	1 cut(s)	91
BspHI	TCATGA	1 cut(s)	1588
BspLI	GGNNCC	9 cut(s)	21, 87, 915, 959, 1044, 1373, 1761, 1974, 2184
BspMAI	CTGCAG	1 cut(s)	463
BspMI	ACCTGC	1 cut(s)	1192
BspPI	GGATC	3 cut(s)	204, 1423, 1798
BspT107I	GGYRCC	1 cut(s)	2182
BspTNI	GGTCTC	1 cut(s)	110
BsrBI	CCGCTC	1 cut(s)	1074
BsrDI	GCAATG	1 cut(s)	818
BsrFI	RCCGGY	1 cut(s)	7
BsrI	ACTGG	6 cut(s)	208, 727, 849, 1982, 2198, 2328
BssAI	RCCGGY	1 cut(s)	7
BssECI	CCNNGG	5 cut(s)	356, 357, 1382, 1383, 2240
BssNAI	GTATAC	1 cut(s)	687
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	1 cut(s)	2240
Bst1107I	GTATAC	1 cut(s)	687
Bst2UI	CCWGG	2 cut(s)	358, 1384
Bst4CI	ACNGT	4 cut(s)	220, 380, 684, 995
Bst6I	CTCTTC	4 cut(s)	664, 1104, 2112, 2142
BstACI	GRCGYC	1 cut(s)	35
BstC8I	GCNNGC	4 cut(s)	9, 188, 857, 2003
BstENI	CCTNNNNNAGG	1 cut(s)	1030
BstF5I	GGATG	8 cut(s)	278, 781, 1058, 1271, 1497, 2142, 2215, 2310
BstFNI	CGCG	1 cut(s)	91
BstH2I	RGCGCY	1 cut(s)	1175
BstHHI	GCGC	2 cut(s)	93, 1174
BstMAI	GTCTC	3 cut(s)	110, 969, 1232
BstMWI	GCNNNNNNNGC	3 cut(s)	389, 1042, 2296
BstNI	CCWGG	2 cut(s)	358, 1384
BstNSI	RCATGY	4 cut(s)	190, 232, 859, 1517
BstSCI	CCNGG	2 cut(s)	356, 1382
BstSFI	CTRYAG	4 cut(s)	300, 432, 459, 2282
BstSLI	GKGCMC	2 cut(s)	1375, 2058
BstUI	CGCG	1 cut(s)	91
BstV1I	GCAGC	4 cut(s)	932, 2141, 2320, 2323
BstX2I	RGATCY	5 cut(s)	209, 562, 785, 1428, 1483
BstYI	RGATCY	5 cut(s)	209, 562, 785, 1428, 1483
BstZ17I	GTATAC	1 cut(s)	687
Bsu36I	CCTNAGG	1 cut(s)	1527
BsuRI	GGCC	7 cut(s)	140, 455, 1036, 1121, 1253, 1373, 2197
BtsCI	GGATG	8 cut(s)	278, 781, 1058, 1271, 1497, 2142, 2215, 2310
BtsI	GCAGTG	3 cut(s)	456, 983, 2058
BtsIMutI	CAGTG	8 cut(s)	385, 456, 856, 983, 1788, 2058, 2205, 2335
BveI	ACCTGC	1 cut(s)	1192
Cac8I	GCNNGC	4 cut(s)	9, 188, 857, 2003
CciI	TCATGA	1 cut(s)	1588
CfoI	GCGC	2 cut(s)	93, 1174
Cfr10I	RCCGGY	1 cut(s)	7
Cfr13I	GGNCC	4 cut(s)	1251, 1371, 1372, 1972
CseI	GACGC	2 cut(s)	24, 2185
Csp6I	GTAC	6 cut(s)	429, 525, 969, 1092, 1200, 2183
CviAII	CATG	9 cut(s)	187, 229, 541, 733, 856, 1514, 1589, 1874, 2189
CviQI	GTAC	6 cut(s)	429, 525, 969, 1092, 1200, 2183
DrdI	GACNNNNNNGTC	1 cut(s)	34
DseDI	GACNNNNNNGTC	1 cut(s)	34
EaeI	YGGCCR	2 cut(s)	453, 1119
Eam1104I	CTCTTC	4 cut(s)	664, 1104, 2112, 2142
EarI	CTCTTC	4 cut(s)	664, 1104, 2112, 2142
EciI	GGCGGA	2 cut(s)	148, 2308
Ecl136II	GAGCTC	1 cut(s)	145
Eco130I	CCWWGG	1 cut(s)	2240
Eco24I	GRGCYC	4 cut(s)	147, 1375, 1626, 1824
Eco31I	GGTCTC	1 cut(s)	110
Eco32I	GATATC	1 cut(s)	606
Eco47I	GGWCC	1 cut(s)	1972
Eco53kI	GAGCTC	1 cut(s)	145
Eco57I	CTGAAG	3 cut(s)	264, 1563, 1740
Eco81I	CCTNAGG	1 cut(s)	1527
Eco88I	CYCGRG	2 cut(s)	1487, 1988
EcoICRI	GAGCTC	1 cut(s)	145
EcoNI	CCTNNNNNAGG	1 cut(s)	1030
EcoO109I	RGGNCCY	2 cut(s)	1371, 1972
EcoRI	GAATTC	2 cut(s)	1226, 2024
EcoRII	CCWGG	2 cut(s)	356, 1382
EcoRV	GATATC	1 cut(s)	606
EcoT14I	CCWWGG	1 cut(s)	2240
EcoT22I	ATGCAT	1 cut(s)	861
EcoT38I	GRGCYC	4 cut(s)	147, 1375, 1626, 1824
ErhI	CCWWGG	1 cut(s)	2240
FaeI	CATG	9 cut(s)	190, 232, 544, 736, 859, 1517, 1592, 1877, 2192
FatI	CATG	9 cut(s)	186, 228, 540, 732, 855, 1513, 1588, 1873, 2188
FauNDI	CATATG	1 cut(s)	1008
FbaI	TGATCA	1 cut(s)	2071
FblI	GTMKAC	1 cut(s)	686
Fnu4HI	GCNGC	7 cut(s)	5, 764, 921, 2130, 2288, 2334, 2337
FokI	GGATG	8 cut(s)	265, 788, 1045, 1258, 1504, 2149, 2202, 2317
FriOI	GRGCYC	4 cut(s)	147, 1375, 1626, 1824
Fsp4HI	GCNGC	7 cut(s)	5, 764, 921, 2130, 2288, 2334, 2337
FspBI	CTAG	4 cut(s)	192, 789, 1755, 1856
GlaI	GCGC	2 cut(s)	92, 1173
GluI	GCNGC	7 cut(s)	5, 764, 921, 2130, 2288, 2334, 2337
GsaI	CCCAGC	1 cut(s)	1066
HaeII	RGCGCY	1 cut(s)	1175
HaeIII	GGCC	7 cut(s)	140, 455, 1036, 1121, 1253, 1373, 2197
HapII	CCGG	1 cut(s)	8
HgaI	GACGC	2 cut(s)	24, 2185
HhaI	GCGC	2 cut(s)	93, 1174
Hin1I	GRCGYC	1 cut(s)	35
Hin1II	CATG	9 cut(s)	190, 232, 544, 736, 859, 1517, 1592, 1877, 2192
Hin6I	GCGC	2 cut(s)	91, 1172
HinP1I	GCGC	2 cut(s)	91, 1172
HpaII	CCGG	1 cut(s)	8
HphI	GGTGA	4 cut(s)	277, 1015, 1399, 2256
Hpy166II	GTNNAC	6 cut(s)	268, 687, 1153, 1202, 1379, 2056
Hpy188I	TCNGA	5 cut(s)	570, 1455, 1621, 1935, 2034
Hpy8I	GTNNAC	6 cut(s)	268, 687, 1153, 1202, 1379, 2056
Hpy99I	CGWCG	9 cut(s)	16, 34, 46, 49, 52, 55, 68, 125, 128
HpyCH4III	ACNGT	4 cut(s)	220, 380, 684, 995
HpyCH4IV	ACGT	2 cut(s)	1352, 1394
HpyF10VI	GCNNNNNNNGC	3 cut(s)	389, 1042, 2296
HpySE526I	ACGT	2 cut(s)	1352, 1394
Hsp92I	GRCGYC	1 cut(s)	35
Hsp92II	CATG	9 cut(s)	190, 232, 544, 736, 859, 1517, 1592, 1877, 2192
HspAI	GCGC	2 cut(s)	91, 1172
KpnI	GGTACC	1 cut(s)	2186
KroI	GCCGGC	1 cut(s)	7
KroNI	GCCGGC	1 cut(s)	9
Ksp22I	TGATCA	1 cut(s)	2071
LmnI	GCTCC	3 cut(s)	91, 1042, 1691
Lsp1109I	GCAGC	4 cut(s)	932, 2141, 2320, 2323
LweI	GCATC	1 cut(s)	2224
MaeI	CTAG	4 cut(s)	192, 789, 1755, 1856
MaeII	ACGT	2 cut(s)	1352, 1394
MaeIII	GTNAC	5 cut(s)	647, 981, 1353, 1516, 2262
MbiI	CCGCTC	1 cut(s)	1074
MfeI	CAATTG	1 cut(s)	1736
MflI	RGATCY	5 cut(s)	209, 562, 785, 1428, 1483
MhlI	GDGCHC	5 cut(s)	147, 1375, 1626, 1824, 2058
MlsI	TGGCCA	2 cut(s)	455, 1121
MluNI	TGGCCA	2 cut(s)	455, 1121
MlyI	GAGTC	2 cut(s)	149, 326
MmeI	TCCRAC	3 cut(s)	753, 1373, 1876
Mox20I	TGGCCA	2 cut(s)	455, 1121
Mph1103I	ATGCAT	1 cut(s)	861
MreI	CGCCGGCG	1 cut(s)	7
MroNI	GCCGGC	1 cut(s)	7
MroXI	GAANNNNTTC	2 cut(s)	351, 956
MscI	TGGCCA	2 cut(s)	455, 1121
MseI	TTAA	7 cut(s)	759, 801, 876, 1028, 1137, 2040, 2082
MslI	CAYNNNNRTG	1 cut(s)	731
Msp20I	TGGCCA	2 cut(s)	455, 1121
MspA1I	CMGCKG	2 cut(s)	287, 2287
MspI	CCGG	1 cut(s)	8
MspR9I	CCNGG	2 cut(s)	358, 1384
MunI	CAATTG	1 cut(s)	1736
Mva1269I	GAATGC	2 cut(s)	859, 1835
MvaI	CCWGG	2 cut(s)	358, 1384
MvnI	CGCG	1 cut(s)	91
MwoI	GCNNNNNNNGC	3 cut(s)	389, 1042, 2296
NaeI	GCCGGC	1 cut(s)	9
NdeI	CATATG	1 cut(s)	1008
NgoMIV	GCCGGC	1 cut(s)	7
NlaIII	CATG	9 cut(s)	190, 232, 544, 736, 859, 1517, 1592, 1877, 2192
NlaIV	GGNNCC	9 cut(s)	21, 87, 915, 959, 1044, 1373, 1761, 1974, 2184
NmeAIII	GCCGAG	3 cut(s)	62, 166, 1123
NmuCI	GTSAC	4 cut(s)	647, 981, 1516, 2262
NsiI	ATGCAT	1 cut(s)	861
NspI	RCATGY	4 cut(s)	190, 232, 859, 1517
PaeI	GCATGC	2 cut(s)	190, 859
PagI	TCATGA	1 cut(s)	1588
PasI	CCCWGGG	2 cut(s)	357, 1383
PciI	ACATGT	1 cut(s)	1513
PcsI	WCGNNNNNNNCGW	4 cut(s)	38, 47, 50, 120
PctI	GAATGC	2 cut(s)	859, 1835
PdiI	GCCGGC	1 cut(s)	9
PdmI	GAANNNNTTC	2 cut(s)	351, 956
PfeI	GAWTC	8 cut(s)	195, 275, 296, 575, 748, 796, 1162, 1633
PflFI	GACNNNGTC	2 cut(s)	34, 316
PkrI	GCNGC	7 cut(s)	6, 765, 922, 2131, 2289, 2335, 2338
PleI	GAGTC	2 cut(s)	149, 325
PpsI	GAGTC	2 cut(s)	149, 325
PpuMI	RGGWCCY	1 cut(s)	1972
PscI	ACATGT	1 cut(s)	1513
Psp124BI	GAGCTC	1 cut(s)	147
Psp1406I	AACGTT	1 cut(s)	1352
Psp5II	RGGWCCY	1 cut(s)	1972
Psp6I	CCWGG	2 cut(s)	356, 1382
PspFI	CCCAGC	1 cut(s)	1062
PspGI	CCWGG	2 cut(s)	356, 1382
PspN4I	GGNNCC	9 cut(s)	21, 87, 915, 959, 1044, 1373, 1761, 1974, 2184
PspOMI	GGGCCC	1 cut(s)	1371
PspPI	GGNCC	4 cut(s)	1251, 1371, 1372, 1972
PspPPI	RGGWCCY	1 cut(s)	1972
PstI	CTGCAG	1 cut(s)	463
PsuI	RGATCY	5 cut(s)	209, 562, 785, 1428, 1483
PsyI	GACNNNGTC	2 cut(s)	34, 316
PvuII	CAGCTG	1 cut(s)	287
RsaI	GTAC	6 cut(s)	430, 526, 970, 1093, 1201, 2184
RsaNI	GTAC	6 cut(s)	429, 525, 969, 1092, 1200, 2183
RseI	CAYNNNNRTG	1 cut(s)	731
SacI	GAGCTC	1 cut(s)	147
SaqAI	TTAA	7 cut(s)	759, 801, 876, 1028, 1137, 2040, 2082
SatI	GCNGC	7 cut(s)	5, 764, 921, 2130, 2288, 2334, 2337
Sau96I	GGNCC	4 cut(s)	1251, 1371, 1372, 1972
ScaI	AGTACT	1 cut(s)	1093
SchI	GAGTC	2 cut(s)	149, 326
ScrFI	CCNGG	2 cut(s)	358, 1384
SduI	GDGCHC	5 cut(s)	147, 1375, 1626, 1824, 2058
SfaNI	GCATC	1 cut(s)	2224
SfcI	CTRYAG	4 cut(s)	300, 432, 459, 2282
SgrAI	CRCCGGYG	1 cut(s)	7
SinI	GGWCC	1 cut(s)	1972
SmiMI	CAYNNNNRTG	1 cut(s)	731
SphI	GCATGC	2 cut(s)	190, 859
SsiI	CCGC	7 cut(s)	5, 89, 159, 763, 1072, 2287, 2319
SspMI	CTAG	4 cut(s)	192, 789, 1755, 1856
SstI	GAGCTC	1 cut(s)	147
StyD4I	CCNGG	2 cut(s)	356, 1382
StyI	CCWWGG	1 cut(s)	2240
TaaI	ACNGT	4 cut(s)	220, 380, 684, 995
TaiI	ACGT	2 cut(s)	1355, 1397
TaqI	TCGA	7 cut(s)	41, 63, 99, 153, 320, 1144, 1316
TaqII	GACCGA	1 cut(s)	134
TatI	WGTACW	4 cut(s)	428, 524, 1091, 1199
TauI	GCSGC	3 cut(s)	7, 766, 2290
TfiI	GAWTC	8 cut(s)	195, 275, 296, 575, 748, 796, 1162, 1633
Tru1I	TTAA	7 cut(s)	759, 801, 876, 1028, 1137, 2040, 2082
Tru9I	TTAA	7 cut(s)	759, 801, 876, 1028, 1137, 2040, 2082
TscAI	CASTG	8 cut(s)	385, 463, 856, 990, 1795, 2058, 2205, 2335
TseFI	GTSAC	4 cut(s)	647, 981, 1516, 2262
TseI	GCWGC	4 cut(s)	920, 2129, 2333, 2336
Tsp45I	GTSAC	4 cut(s)	647, 981, 1516, 2262
TspDTI	ATGAA	9 cut(s)	94, 149, 456, 557, 707, 965, 1025, 1566, 1577
TspGWI	ACGGA	3 cut(s)	29, 505, 1924
TspRI	CASTG	8 cut(s)	385, 463, 856, 990, 1795, 2058, 2205, 2335
Tth111I	GACNNNGTC	2 cut(s)	34, 316
VneI	GTGCAC	1 cut(s)	2054
VpaK11BI	GGWCC	1 cut(s)	1972
XagI	CCTNNNNNAGG	1 cut(s)	1030
XapI	RAATTY	8 cut(s)	1049, 1125, 1226, 1436, 1532, 1709, 2024, 2066
XbaI	TCTAGA	1 cut(s)	788
XceI	RCATGY	4 cut(s)	190, 232, 859, 1517
XmiI	GTMKAC	1 cut(s)	686
XmnI	GAANNNNTTC	2 cut(s)	351, 956
XspI	CTAG	4 cut(s)	192, 789, 1755, 1856
ZrmI	AGTACT	1 cut(s)	1093
Zsp2I	ATGCAT	1 cut(s)	861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.