FvH4_6g27220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002484
Physical Location & Seq
Forward (+)
20899023 .. 20900696
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27220.t2

Sequence Viewer

Length: 552 bp
ATGACGAAGACGAAGAGCCAGAGCGACAAGATGTCTCTGGAAGACTACCTCCTTCTCATTCAGTCTCGCTCCACCCTCCACCTCACCGTCACTCAACTCAATCAGATCATCAGCATGCACGGATTCAAAAAGCTTCACAGAATTCCCAAGAAAGATCTGAGCGAAGCCGTGAGCACACTGGACCTGGAGGAACCGAGCCGCTCTACACTCCGGGACTACATCTCGCCGGTGGTCCACACTTGTCCGAACGACGTCGTCGTCGCTGACATGAACGCCCTGAGCTGGCAAGAGTGCTGCATCACCGCCGTCCACACGTTGAGCTCGTGTCATCAAAACGACGCCGTCGAGTGCTCGAACAATCACCGCTCCGGTGCTACTTCATCTCAAGGAAAGACGCCATTGAAGAAATTGGTGCAGAGGAGGAAGAGACGGACTCAGGAGAGCGGCGGTGCGGTGGCGAAGAAGCAGGGGAAGAGATCGGAAAACAACGGTGGCGGCGGCGCTACTCTTGCTTTGGATTGTGTTTCGATGGATTCGTATTCTTTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.13

Weight (kDa)

9.07

Isoelectric Point (pI)

66.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 1 - 24 3.8e-09 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AatII GACGTC 1 cut(s) 255
AccBSI CCGCTC 3 cut(s) 201, 366, 444
AciI CCGC 8 cut(s) 199, 303, 364, 444, 447, 452, 495, 498
AcsI RAATTY 1 cut(s) 141
AcyI GRCGYC 3 cut(s) 252, 339, 395
AfiI CCNNNNNNNGG 1 cut(s) 282
AflIII ACRYGT 1 cut(s) 312
AgsI TTSAA 2 cut(s) 127, 403
AhdI GACNNNNNGTC 1 cut(s) 31
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 3 cut(s) 133, 282, 321
AluI AGCT 3 cut(s) 133, 282, 321
Alw21I GWGCWC 3 cut(s) 176, 323, 353
Alw26I GTCTC 3 cut(s) 39, 69, 421
ApeKI GCWGC 1 cut(s) 294
ApoI RAATTY 1 cut(s) 141
AspLEI GCGC 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 181, 232
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 3 cut(s) 76, 292, 353
AvaII GGWCC 2 cut(s) 181, 232
BanII GRGCYC 1 cut(s) 323
BauI CACGAG 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 14, 48
Bbv12I GWGCWC 3 cut(s) 176, 323, 353
BbvI GCAGC 1 cut(s) 281
BccI CCATC 1 cut(s) 523
BceAI ACGGC 3 cut(s) 152, 290, 326
BciT130I CCWGG 1 cut(s) 185
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 3 cut(s) 39, 69, 421
BfoI RGCGCY 1 cut(s) 504
BglII AGATCT 1 cut(s) 154
BisI GCNGC 5 cut(s) 199, 295, 445, 496, 499
BlsI GCNGC 5 cut(s) 200, 296, 446, 497, 500
Bme1390I CCNGG 2 cut(s) 185, 212
Bme18I GGWCC 2 cut(s) 181, 232
BmeRI GACNNNNNGTC 1 cut(s) 31
BmgT120I GGNCC 2 cut(s) 181, 232
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 2 cut(s) 185, 212
BmsI GCATC 1 cut(s) 306
BpiI GAAGAC 2 cut(s) 14, 48
BplI GAGNNNNNCTC 2 cut(s) 418, 450
BpmI CTGGAG 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 212
BsaHI GRCGYC 3 cut(s) 252, 339, 395
BsaWI WCCGGW 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 183
BseBI CCWGG 1 cut(s) 185
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMII CTCAG 3 cut(s) 149, 269, 449
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 1 cut(s) 433
BseXI GCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 434
BsiHKAI GWGCWC 3 cut(s) 176, 323, 353
BsiSI CCGG 3 cut(s) 211, 227, 369
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 3 cut(s) 39, 69, 421
BsmBI CGTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 227
Bsp1286I GDGCHC 3 cut(s) 176, 323, 353
Bsp143I GATC 3 cut(s) 105, 154, 476
BspACI CCGC 8 cut(s) 199, 303, 364, 444, 447, 452, 495, 498
BspCNI CTCAG 3 cut(s) 150, 270, 448
BspLI GGNNCC 1 cut(s) 192
BspQI GCTCTTC 1 cut(s) 8
BsrBI CCGCTC 3 cut(s) 201, 366, 444
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 183
BssAI RCCGGY 1 cut(s) 226
BssMI GATC 3 cut(s) 105, 154, 476
BssNI GRCGYC 3 cut(s) 252, 339, 395
BssSI CACGAG 1 cut(s) 322
Bst2BI CACGAG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 2 cut(s) 88, 491
Bst6I CTCTTC 3 cut(s) 8, 419, 467
BstACI GRCGYC 3 cut(s) 252, 339, 395
BstC8I GCNNGC 2 cut(s) 116, 284
BstDEI CTNAG 3 cut(s) 158, 278, 435
BstH2I RGCGCY 1 cut(s) 504
BstHHI GCGC 1 cut(s) 503
BstKTI GATC 3 cut(s) 108, 157, 479
BstMAI GTCTC 3 cut(s) 39, 69, 421
BstMBI GATC 3 cut(s) 105, 154, 476
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 2 cut(s) 183, 210
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 2 cut(s) 14, 48
BstX2I RGATCY 1 cut(s) 154
BstYI RGATCY 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 176
Cac8I GCNNGC 2 cut(s) 116, 284
CfoI GCGC 1 cut(s) 503
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 181, 232
CseI GACGC 2 cut(s) 347, 403
CviAII CATG 2 cut(s) 115, 268
CviJI RGCY 6 cut(s) 18, 133, 167, 198, 282, 321
CviKI_1 RGCY 6 cut(s) 18, 133, 167, 198, 282, 321
DdeI CTNAG 3 cut(s) 158, 278, 435
DpnI GATC 3 cut(s) 107, 156, 478
DpnII GATC 3 cut(s) 105, 154, 476
DrdI GACNNNNNNGTC 1 cut(s) 257
DriI GACNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 257
Eam1104I CTCTTC 3 cut(s) 8, 419, 467
Eam1105I GACNNNNNGTC 1 cut(s) 31
EarI CTCTTC 3 cut(s) 8, 419, 467
Ecl136II GAGCTC 1 cut(s) 321
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 2 cut(s) 181, 232
Eco53kI GAGCTC 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 141
EcoRII CCWGG 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 323
Esp3I CGTCTC 1 cut(s) 421
FaeI CATG 2 cut(s) 118, 271
FaiI YATR 2 cut(s) 116, 269
FaqI GGGAC 1 cut(s) 227
FatI CATG 2 cut(s) 114, 267
Fnu4HI GCNGC 5 cut(s) 199, 295, 445, 496, 499
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 5 cut(s) 199, 295, 445, 496, 499
GlaI GCGC 1 cut(s) 502
GluI GCNGC 5 cut(s) 199, 295, 445, 496, 499
GsuI CTGGAG 1 cut(s) 206
HaeII RGCGCY 1 cut(s) 504
HapII CCGG 3 cut(s) 211, 227, 369
HgaI GACGC 2 cut(s) 347, 403
HhaI GCGC 1 cut(s) 503
Hin1I GRCGYC 3 cut(s) 252, 339, 395
Hin1II CATG 2 cut(s) 118, 271
Hin6I GCGC 1 cut(s) 501
HinP1I GCGC 1 cut(s) 501
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 3 cut(s) 123, 433, 533
HpaII CCGG 3 cut(s) 211, 227, 369
HphI GGTGA 3 cut(s) 76, 292, 353
Hpy166II GTNNAC 2 cut(s) 235, 310
Hpy188I TCNGA 4 cut(s) 105, 159, 246, 481
Hpy188III TCNNGA 2 cut(s) 38, 437
Hpy8I GTNNAC 2 cut(s) 235, 310
Hpy99I CGWCG 6 cut(s) 254, 257, 260, 263, 341, 347
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 88, 491
HpyCH4IV ACGT 2 cut(s) 252, 314
HpyCH4V TGCA 3 cut(s) 118, 297, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 3 cut(s) 158, 278, 435
HpySE526I ACGT 2 cut(s) 252, 314
Hsp92I GRCGYC 3 cut(s) 252, 339, 395
Hsp92II CATG 2 cut(s) 118, 271
HspAI GCGC 1 cut(s) 501
Kzo9I GATC 3 cut(s) 105, 154, 476
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 2 cut(s) 74, 371
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 1 cut(s) 306
MaeII ACGT 2 cut(s) 252, 314
MaeIII GTNAC 1 cut(s) 88
MalI GATC 3 cut(s) 107, 156, 478
MbiI CCGCTC 3 cut(s) 201, 366, 444
MboI GATC 3 cut(s) 105, 154, 476
MboII GAAGA 7 cut(s) 19, 25, 53, 415, 436, 472, 484
MflI RGATCY 1 cut(s) 154
MhlI GDGCHC 3 cut(s) 176, 323, 353
MluCI AATT 2 cut(s) 141, 407
MlyI GAGTC 1 cut(s) 427
MnlI CCTC 6 cut(s) 59, 86, 92, 181, 411, 414
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 3 cut(s) 211, 227, 369
MspR9I CCNGG 2 cut(s) 185, 212
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 509
NciI CCSGG 1 cut(s) 212
NdeII GATC 3 cut(s) 105, 154, 476
NlaIII CATG 2 cut(s) 118, 271
NlaIV GGNNCC 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 88
NspI RCATGY 1 cut(s) 118
PaeI GCATGC 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 4 cut(s) 255, 320, 342, 533
PfeI GAWTC 2 cut(s) 123, 533
PflFI GACNNNGTC 2 cut(s) 254, 341
PfoI TCCNGGA 1 cut(s) 210
PkrI GCNGC 5 cut(s) 200, 296, 446, 497, 500
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
Psp124BI GAGCTC 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 2 cut(s) 181, 232
PsuI RGATCY 1 cut(s) 154
PsyI GACNNNGTC 2 cut(s) 254, 341
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 1 cut(s) 323
SapI GCTCTTC 1 cut(s) 8
SatI GCNGC 5 cut(s) 199, 295, 445, 496, 499
Sau3AI GATC 3 cut(s) 105, 154, 476
Sau96I GGNCC 2 cut(s) 181, 232
SchI GAGTC 1 cut(s) 427
ScrFI CCNGG 2 cut(s) 185, 212
SduI GDGCHC 3 cut(s) 176, 323, 353
SetI ASST 8 cut(s) 51, 84, 135, 186, 255, 284, 317, 323
SfaNI GCATC 1 cut(s) 306
SgrAI CRCCGGYG 1 cut(s) 226
SinI GGWCC 2 cut(s) 181, 232
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 384
SmoI CTYRAG 1 cut(s) 384
SphI GCATGC 1 cut(s) 118
Sse9I AATT 2 cut(s) 141, 407
SsiI CCGC 8 cut(s) 199, 303, 364, 444, 447, 452, 495, 498
SstI GAGCTC 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 183, 210
TaaI ACNGT 2 cut(s) 88, 491
TaiI ACGT 2 cut(s) 255, 317
TaqI TCGA 3 cut(s) 345, 353, 527
TasI AATT 2 cut(s) 141, 407
TauI GCSGC 4 cut(s) 201, 447, 498, 501
TfiI GAWTC 2 cut(s) 123, 533
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 1 cut(s) 294
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 2 cut(s) 284, 369
TspGWI ACGGA 2 cut(s) 135, 445
TspRI CASTG 1 cut(s) 183
Tth111I GACNNNGTC 2 cut(s) 254, 341
VpaK11BI GGWCC 2 cut(s) 181, 232
XapI RAATTY 1 cut(s) 141
XceI RCATGY 1 cut(s) 118
ZraI GACGTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.