Rw2G028000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002484
Physical Location & Seq
Reverse (-)
44554351 .. 44555871
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G028000.1

Sequence Viewer

Length: 378 bp
ATGCACGGCTACAAGAAGATCCACAAAGTTCACAAGAAGCTTCTGAGCGACGCCGTGAACTCACTGCAGCTGGTGAACCCGTGCCGCTCCACGCTTAGGGACTACATCTCGCCGTTTGTGACGACGAAGATGGATGACGTCTTCGCCGACCTCAGCGATCTCAACTGGCAAGACTGTTGCATCACCGCCATCCACACCCTCAGTTCGTGGGAGGACCAGTCCCCTCCTCCTCCAAACGGCGCCGTCCGGTTCCAACACTACTCGCCGTCCACCGCAGCCCACAGCGTGATCGTTTCTGCTCCCGGACCGACCAAGAAGAGATCGGCGAGGAAGAGGAAGCGGACTGCTAAAGTGGCCGATCTGGTAAATTTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.96

Weight (kDa)

9.67

Isoelectric Point (pI)

60.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 1 - 26 1.4e-08 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 239
AccBSI CCGCTC 1 cut(s) 87
AciI CCGC 4 cut(s) 85, 186, 273, 340
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 1 cut(s) 367
AcyI GRCGYC 3 cut(s) 51, 138, 240
AdeI CACNNNGTG 1 cut(s) 286
AfiI CCNNNNNNNGG 2 cut(s) 96, 236
AluBI AGCT 2 cut(s) 40, 70
AluI AGCT 2 cut(s) 40, 70
AlwI GGATC 1 cut(s) 13
AoxI GGCC 1 cut(s) 354
ApeKI GCWGC 2 cut(s) 67, 275
ApoI RAATTY 1 cut(s) 367
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 214, 305
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 85, 175
AvaII GGWCC 2 cut(s) 214, 305
BanI GGYRCC 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 133
BbvCI CCTCAGC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 79, 287
BccI CCATC 2 cut(s) 124, 197
BceAI ACGGC 6 cut(s) 22, 38, 97, 227, 250, 253
BcnI CCSGG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 3 cut(s) 68, 85, 276
BlsI GCNGC 3 cut(s) 69, 86, 277
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 2 cut(s) 214, 305
BmgT120I GGNCC 2 cut(s) 214, 305
BmiI GGNNCC 2 cut(s) 241, 251
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 1 cut(s) 189
BpiI GAAGAC 1 cut(s) 133
Bpu10I CCTNAGC 2 cut(s) 95, 152
BpuMI CCSGG 1 cut(s) 303
BsaHI GRCGYC 3 cut(s) 51, 138, 240
BsaWI WCCGGW 1 cut(s) 246
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 236
Bse1I ACTGG 2 cut(s) 170, 217
BseGI GGATG 2 cut(s) 139, 189
BseLI CCNNNNNNNGG 2 cut(s) 96, 236
BseMII CTCAG 3 cut(s) 35, 166, 214
BseNI ACTGG 2 cut(s) 170, 217
BseRI GAGGAG 2 cut(s) 216, 219
BseXI GCAGC 2 cut(s) 79, 287
BshFI GGCC 1 cut(s) 356
BshNI GGYRCC 1 cut(s) 239
BsiSI CCGG 2 cut(s) 247, 303
BslFI GGGAC 2 cut(s) 113, 205
BslI CCNNNNNNNGG 2 cut(s) 96, 236
BsmFI GGGAC 2 cut(s) 113, 205
BsnI GGCC 1 cut(s) 356
Bsp143I GATC 5 cut(s) 18, 157, 288, 320, 358
BspACI CCGC 4 cut(s) 85, 186, 273, 340
BspANI GGCC 1 cut(s) 356
BspCNI CTCAG 3 cut(s) 36, 165, 213
BspLI GGNNCC 2 cut(s) 241, 251
BspMAI CTGCAG 1 cut(s) 69
BspPI GGATC 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 87
BsrI ACTGG 2 cut(s) 170, 217
BssMI GATC 5 cut(s) 18, 157, 288, 320, 358
BssNI GRCGYC 3 cut(s) 51, 138, 240
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 2 cut(s) 311, 326
BstACI GRCGYC 3 cut(s) 51, 138, 240
BstDEI CTNAG 4 cut(s) 44, 95, 152, 200
BstF5I GGATG 2 cut(s) 139, 189
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 5 cut(s) 21, 160, 291, 323, 361
BstMBI GATC 5 cut(s) 18, 157, 288, 320, 358
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 1 cut(s) 65
BstV1I GCAGC 2 cut(s) 79, 287
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 1 cut(s) 356
BtsCI GGATG 2 cut(s) 139, 189
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 62
CfoI GCGC 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 214, 305
CpoI CGGWCCG 1 cut(s) 305
CseI GACGC 1 cut(s) 59
CspI CGGWCCG 1 cut(s) 305
CviJI RGCY 5 cut(s) 9, 40, 70, 278, 356
CviKI_1 RGCY 5 cut(s) 9, 40, 70, 278, 356
DdeI CTNAG 4 cut(s) 44, 95, 152, 200
DinI GGCGCC 1 cut(s) 241
DpnI GATC 5 cut(s) 20, 159, 290, 322, 360
DpnII GATC 5 cut(s) 18, 157, 288, 320, 358
DraIII CACNNNGTG 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 2 cut(s) 311, 326
EarI CTCTTC 2 cut(s) 311, 326
Eco47I GGWCC 2 cut(s) 214, 305
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
FaqI GGGAC 2 cut(s) 113, 205
Fnu4HI GCNGC 3 cut(s) 68, 85, 276
FokI GGATG 2 cut(s) 146, 176
Fsp4HI GCNGC 3 cut(s) 68, 85, 276
GlaI GCGC 1 cut(s) 241
GluI GCNGC 3 cut(s) 68, 85, 276
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 1 cut(s) 356
HapII CCGG 2 cut(s) 247, 303
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 242
Hin1I GRCGYC 3 cut(s) 51, 138, 240
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HindIII AAGCTT 1 cut(s) 38
HpaII CCGG 2 cut(s) 247, 303
HphI GGTGA 2 cut(s) 85, 175
Hpy166II GTNNAC 4 cut(s) 31, 58, 76, 270
Hpy188I TCNGA 1 cut(s) 45
Hpy8I GTNNAC 4 cut(s) 31, 58, 76, 270
Hpy99I CGWCG 2 cut(s) 53, 127
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 3 cut(s) 4, 67, 180
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 4 cut(s) 44, 95, 152, 200
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 3 cut(s) 51, 138, 240
HspAI GCGC 1 cut(s) 240
KasI GGCGCC 1 cut(s) 239
Kzo9I GATC 5 cut(s) 18, 157, 288, 320, 358
LmnI GCTCC 2 cut(s) 92, 304
LpnPI CCDG 6 cut(s) 56, 151, 230, 260, 316, 347
Lsp1109I GCAGC 2 cut(s) 79, 287
LweI GCATC 1 cut(s) 189
MaeII ACGT 1 cut(s) 138
MaeIII GTNAC 1 cut(s) 118
MalI GATC 5 cut(s) 20, 159, 290, 322, 360
MbiI CCGCTC 1 cut(s) 87
MboI GATC 5 cut(s) 18, 157, 288, 320, 358
MboII GAAGA 5 cut(s) 28, 133, 139, 328, 343
MflI RGATCY 1 cut(s) 18
MluCI AATT 1 cut(s) 367
Mly113I GGCGCC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 8 cut(s) 161, 205, 209, 234, 237, 240, 321, 327
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 2 cut(s) 247, 303
MspR9I CCNGG 1 cut(s) 303
MwoI GCNNNNNNNGC 1 cut(s) 353
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 1 cut(s) 303
NdeII GATC 5 cut(s) 18, 157, 288, 320, 358
NlaIV GGNNCC 2 cut(s) 241, 251
NmuCI GTSAC 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 3 cut(s) 69, 86, 277
PluTI GGCGCC 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 241, 251
PspPI GGNCC 2 cut(s) 214, 305
PstI CTGCAG 1 cut(s) 69
PsuI RGATCY 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 70
Rsr2I CGGWCCG 1 cut(s) 305
RsrII CGGWCCG 1 cut(s) 305
SatI GCNGC 3 cut(s) 68, 85, 276
Sau3AI GATC 5 cut(s) 18, 157, 288, 320, 358
Sau96I GGNCC 2 cut(s) 214, 305
ScrFI CCNGG 1 cut(s) 303
SetI ASST 4 cut(s) 42, 72, 141, 153
SfaNI GCATC 1 cut(s) 189
SfcI CTRYAG 1 cut(s) 65
SfoI GGCGCC 1 cut(s) 241
SinI GGWCC 2 cut(s) 214, 305
Sse9I AATT 1 cut(s) 367
SsiI CCGC 4 cut(s) 85, 186, 273, 340
SspDI GGCGCC 1 cut(s) 239
StyD4I CCNGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 176
TaiI ACGT 1 cut(s) 141
TaqII GACCGA 1 cut(s) 322
TasI AATT 1 cut(s) 367
TauI GCSGC 1 cut(s) 87
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 2 cut(s) 67, 275
Tsp45I GTSAC 1 cut(s) 118
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 2 cut(s) 214, 305
XapI RAATTY 1 cut(s) 367
ZraI GACGTC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.