Rroxscaffold_2G00114680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002484
Physical Location & Seq
Reverse (-)
40839645 .. 40841555
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00114680.1

Sequence Viewer

Length: 477 bp
ATGCTGATGGAGATGGCGAGCAAAGCTAAGAACAACGCGAATGCGAAGATGTCTCTGGATGATTACCTCCTTCTCCTCCAGTCTGGCTCCCACCTCCACCTCACCGTCACCCATCTCAATCAGAAGCTTCTGAGCGACGCCGTGAACTCACTGCAGCTGGTGAACCCGTGCCGCTCCACGCTGAGGGACTACATCTCGCCGTTGGTGACGACGAAGATGGATGACGTCTTCGCCGACCTCAGCGATCTCAACTGGCAAGACTGTTGCATCACGTCCATCCAAACCCTCAGTTCGTGGGAGGACCGGCCCCCTCCTCACCGAAACGGCGCCGTCCAGTTCCAACACCACTCGCCGTCCACCGCTCCGGACTCCGCAGCCCACAGCGTGATCGTTTCTGCTCCCGGACCGACCAAGAAGAGATCGGCGCGGAAGAGGAAGCGGACTGCTAAAGTGGCCGATCTGGTAAATTTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.46

Weight (kDa)

9.1

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 14 - 41 1e-05 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 326
AccBSI CCGCTC 2 cut(s) 174, 362
AccII CGCG 2 cut(s) 38, 427
AccIII TCCGGA 1 cut(s) 364
AciI CCGC 5 cut(s) 172, 360, 372, 427, 439
AcoI YGGCCR 1 cut(s) 453
AcsI RAATTY 1 cut(s) 466
AcyI GRCGYC 3 cut(s) 138, 225, 327
AdeI CACNNNGTG 1 cut(s) 385
AfiI CCNNNNNNNGG 1 cut(s) 183
AjiI CACGTC 1 cut(s) 273
AluBI AGCT 3 cut(s) 26, 127, 157
AluI AGCT 3 cut(s) 26, 127, 157
Alw26I GTCTC 1 cut(s) 57
Aor13HI TCCGGA 1 cut(s) 364
AoxI GGCC 2 cut(s) 305, 453
ApeKI GCWGC 2 cut(s) 154, 374
ApoI RAATTY 1 cut(s) 466
AspLEI GCGC 2 cut(s) 329, 427
AspS9I GGNCC 3 cut(s) 301, 306, 404
AsuC2I CCSGG 1 cut(s) 402
AsuHPI GGTGA 5 cut(s) 94, 100, 172, 217, 308
AvaII GGWCC 2 cut(s) 301, 404
BanI GGYRCC 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 220
BbvCI CCTCAGC 2 cut(s) 182, 239
BbvI GCAGC 2 cut(s) 166, 386
BccI CCATC 4 cut(s) 7, 120, 211, 284
BceAI ACGGC 5 cut(s) 125, 184, 314, 337, 340
BcnI CCSGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 152
BfoI RGCGCY 1 cut(s) 330
BisI GCNGC 3 cut(s) 155, 172, 375
BlsI GCNGC 3 cut(s) 156, 173, 376
Bme1390I CCNGG 1 cut(s) 402
Bme18I GGWCC 2 cut(s) 301, 404
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 3 cut(s) 301, 306, 404
BmiI GGNNCC 3 cut(s) 88, 308, 328
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 2 cut(s) 182, 239
BpuMI CCSGG 1 cut(s) 402
BsaHI GRCGYC 3 cut(s) 138, 225, 327
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 1 cut(s) 183
Bse118I RCCGGY 1 cut(s) 303
Bse1I ACTGG 3 cut(s) 79, 257, 334
BseAI TCCGGA 1 cut(s) 364
BseGI GGATG 3 cut(s) 64, 226, 276
BseLI CCNNNNNNNGG 1 cut(s) 183
BseMII CTCAG 4 cut(s) 122, 173, 253, 301
BseNI ACTGG 3 cut(s) 79, 257, 334
BseRI GAGGAG 2 cut(s) 65, 303
BseXI GCAGC 2 cut(s) 166, 386
Bsh1236I CGCG 2 cut(s) 38, 427
BshFI GGCC 2 cut(s) 307, 455
BshNI GGYRCC 1 cut(s) 326
BsiSI CCGG 3 cut(s) 304, 365, 402
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 2 cut(s) 307, 455
Bsp13I TCCGGA 1 cut(s) 364
Bsp143I GATC 4 cut(s) 244, 387, 419, 457
BspACI CCGC 5 cut(s) 172, 360, 372, 427, 439
BspANI GGCC 2 cut(s) 307, 455
BspCNI CTCAG 4 cut(s) 123, 174, 252, 300
BspEI TCCGGA 1 cut(s) 364
BspFNI CGCG 2 cut(s) 38, 427
BspLI GGNNCC 3 cut(s) 88, 308, 328
BspMAI CTGCAG 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 326
BsrBI CCGCTC 2 cut(s) 174, 362
BsrFI RCCGGY 1 cut(s) 303
BsrI ACTGG 3 cut(s) 79, 257, 334
BssAI RCCGGY 1 cut(s) 303
BssMI GATC 4 cut(s) 244, 387, 419, 457
BssNI GRCGYC 3 cut(s) 138, 225, 327
Bst4CI ACNGT 2 cut(s) 106, 263
Bst6I CTCTTC 2 cut(s) 410, 425
BstACI GRCGYC 3 cut(s) 138, 225, 327
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 5 cut(s) 27, 131, 182, 239, 287
BstF5I GGATG 3 cut(s) 64, 226, 276
BstFNI CGCG 2 cut(s) 38, 427
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 2 cut(s) 329, 427
BstKTI GATC 4 cut(s) 247, 390, 422, 460
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 4 cut(s) 244, 387, 419, 457
BstMWI GCNNNNNNNGC 2 cut(s) 23, 452
BstSCI CCNGG 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 152
BstUI CGCG 2 cut(s) 38, 427
BstV1I GCAGC 2 cut(s) 166, 386
BstV2I GAAGAC 1 cut(s) 220
BsuRI GGCC 2 cut(s) 307, 455
BtrI CACGTC 1 cut(s) 273
BtsCI GGATG 3 cut(s) 64, 226, 276
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 2 cut(s) 329, 427
Cfr10I RCCGGY 1 cut(s) 303
Cfr13I GGNCC 3 cut(s) 301, 306, 404
CpoI CGGWCCG 1 cut(s) 404
CseI GACGC 1 cut(s) 146
CspI CGGWCCG 1 cut(s) 404
CviJI RGCY 7 cut(s) 26, 87, 127, 157, 307, 377, 455
CviKI_1 RGCY 7 cut(s) 26, 87, 127, 157, 307, 377, 455
DdeI CTNAG 5 cut(s) 27, 131, 182, 239, 287
DinI GGCGCC 1 cut(s) 328
DpnI GATC 4 cut(s) 246, 389, 421, 459
DpnII GATC 4 cut(s) 244, 387, 419, 457
DraIII CACNNNGTG 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 2 cut(s) 410, 425
EarI CTCTTC 2 cut(s) 410, 425
Eco47I GGWCC 2 cut(s) 301, 404
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
FaqI GGGAC 1 cut(s) 200
Fnu4HI GCNGC 3 cut(s) 155, 172, 375
FokI GGATG 3 cut(s) 71, 233, 263
Fsp4HI GCNGC 3 cut(s) 155, 172, 375
GlaI GCGC 2 cut(s) 328, 426
GluI GCNGC 3 cut(s) 155, 172, 375
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 2 cut(s) 307, 455
HapII CCGG 3 cut(s) 304, 365, 402
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 329, 427
Hin1I GRCGYC 3 cut(s) 138, 225, 327
Hin6I GCGC 2 cut(s) 327, 425
HinP1I GCGC 2 cut(s) 327, 425
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 1 cut(s) 368
HpaII CCGG 3 cut(s) 304, 365, 402
HphI GGTGA 5 cut(s) 94, 100, 172, 217, 308
Hpy166II GTNNAC 3 cut(s) 145, 163, 357
Hpy188I TCNGA 2 cut(s) 123, 132
Hpy188III TCNNGA 2 cut(s) 56, 365
Hpy8I GTNNAC 3 cut(s) 145, 163, 357
Hpy99I CGWCG 2 cut(s) 140, 214
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 106, 263
HpyCH4IV ACGT 2 cut(s) 225, 272
HpyCH4V TGCA 2 cut(s) 154, 267
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 452
HpyF3I CTNAG 5 cut(s) 27, 131, 182, 239, 287
HpySE526I ACGT 2 cut(s) 225, 272
Hsp92I GRCGYC 3 cut(s) 138, 225, 327
HspAI GCGC 2 cut(s) 327, 425
KasI GGCGCC 1 cut(s) 326
Kpn2I TCCGGA 1 cut(s) 364
Kzo9I GATC 4 cut(s) 244, 387, 419, 457
LmnI GCTCC 4 cut(s) 92, 179, 367, 403
Lsp1109I GCAGC 2 cut(s) 166, 386
LweI GCATC 1 cut(s) 276
MaeII ACGT 2 cut(s) 225, 272
MaeIII GTNAC 2 cut(s) 106, 205
MalI GATC 4 cut(s) 246, 389, 421, 459
MbiI CCGCTC 2 cut(s) 174, 362
MboI GATC 4 cut(s) 244, 387, 419, 457
MboII GAAGA 5 cut(s) 58, 220, 226, 427, 442
MluCI AATT 1 cut(s) 466
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 1 cut(s) 364
MroI TCCGGA 1 cut(s) 364
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 3 cut(s) 304, 365, 402
MspR9I CCNGG 1 cut(s) 402
Mva1269I GAATGC 1 cut(s) 46
MvnI CGCG 2 cut(s) 38, 427
MwoI GCNNNNNNNGC 2 cut(s) 23, 452
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 1 cut(s) 402
NdeII GATC 4 cut(s) 244, 387, 419, 457
NlaIV GGNNCC 3 cut(s) 88, 308, 328
NmuCI GTSAC 2 cut(s) 106, 205
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 400
PkrI GCNGC 3 cut(s) 156, 173, 376
PleI GAGTC 1 cut(s) 362
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 362
PspN4I GGNNCC 3 cut(s) 88, 308, 328
PspPI GGNCC 3 cut(s) 301, 306, 404
PstI CTGCAG 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 157
Rsr2I CGGWCCG 1 cut(s) 404
RsrII CGGWCCG 1 cut(s) 404
SatI GCNGC 3 cut(s) 155, 172, 375
Sau3AI GATC 4 cut(s) 244, 387, 419, 457
Sau96I GGNCC 3 cut(s) 301, 306, 404
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 1 cut(s) 402
SetI ASST 9 cut(s) 28, 69, 96, 102, 129, 159, 228, 240, 275
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 152
SfoI GGCGCC 1 cut(s) 328
SinI GGWCC 2 cut(s) 301, 404
Sse9I AATT 1 cut(s) 466
SsiI CCGC 5 cut(s) 172, 360, 372, 427, 439
SspDI GGCGCC 1 cut(s) 326
StyD4I CCNGG 1 cut(s) 400
TaaI ACNGT 2 cut(s) 106, 263
TaiI ACGT 2 cut(s) 228, 275
TaqII GACCGA 1 cut(s) 421
TasI AATT 1 cut(s) 466
TauI GCSGC 1 cut(s) 174
TscAI CASTG 1 cut(s) 156
TseFI GTSAC 2 cut(s) 106, 205
TseI GCWGC 2 cut(s) 154, 374
Tsp45I GTSAC 2 cut(s) 106, 205
TspRI CASTG 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 301, 404
XapI RAATTY 1 cut(s) 466
ZraI GACGTC 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.