MD08G1181200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002484
Physical Location & Seq
Forward (+)
22489170 .. 22489514
345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1181200.v1.1.491

Sequence Viewer

Length: 345 bp
ATGGTGAGTGCGAGTGCGAGCAAAAGGAAGACGACGAGTTTCCACAGTGAGAAGATGACTCTAGAAGATTACCTGCTCCTCATTCAATCCAACTCTCACCTCCATCTCACCGTCGCTCATCTCAATCAGATCATTTCCATGCACAGATACAAGAAGATCTACAAAGTTCCCAAGGCACGTTTGAGCGACGCCGTGGGCTCTCTGTCGCTAGTTGATCCGGCTCGCTCGACGCTCAGAGACTACATCTCTCCGTTTGGGATCACCACCTTAGAGGACGTCGTCACCGACCTCGCCGACCTCAACTGGAAGGAGTGCCGCGTCACATCCATTGAAACCCAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

115

Amino Acids

12.89

Weight (kDa)

8.76

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 16 - 72 3.1e-22 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc36I ACCTGC 1 cut(s) 81
AccII CGCG 1 cut(s) 318
AciI CCGC 1 cut(s) 316
AclWI GGATC 2 cut(s) 209, 266
AcyI GRCGYC 2 cut(s) 189, 276
AgsI TTSAA 2 cut(s) 86, 332
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 2 cut(s) 209, 266
AsuHPI GGTGA 5 cut(s) 16, 89, 100, 253, 274
BanII GRGCYC 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 35
BccI CCATC 1 cut(s) 111
BceAI ACGGC 1 cut(s) 176
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 2 cut(s) 62, 209
BfuAI ACCTGC 1 cut(s) 81
BglII AGATCT 1 cut(s) 156
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 35
BsaHI GRCGYC 2 cut(s) 189, 276
BsaJI CCNNGG 2 cut(s) 171, 192
Bse1I ACTGG 1 cut(s) 308
BseDI CCNNGG 2 cut(s) 171, 192
BseGI GGATG 1 cut(s) 323
BseMII CTCAG 1 cut(s) 247
BseNI ACTGG 1 cut(s) 308
BseRI GAGGAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 318
BsiSI CCGG 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 4 cut(s) 129, 156, 214, 258
BspACI CCGC 1 cut(s) 316
BspCNI CTCAG 1 cut(s) 246
BspFNI CGCG 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 81
BspPI GGATC 2 cut(s) 209, 266
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 2 cut(s) 171, 192
BssMI GATC 4 cut(s) 129, 156, 214, 258
BssNI GRCGYC 2 cut(s) 189, 276
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 2 cut(s) 47, 112
BstACI GRCGYC 2 cut(s) 189, 276
BstC8I GCNNGC 2 cut(s) 19, 223
BstDEI CTNAG 2 cut(s) 233, 268
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 1 cut(s) 323
BstFNI CGCG 1 cut(s) 318
BstKTI GATC 4 cut(s) 132, 159, 217, 261
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 4 cut(s) 129, 156, 214, 258
BstUI CGCG 1 cut(s) 318
BstV2I GAAGAC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 1 cut(s) 323
BtsIMutI CAGTG 1 cut(s) 52
BveI ACCTGC 1 cut(s) 81
Cac8I GCNNGC 2 cut(s) 19, 223
CseI GACGC 3 cut(s) 197, 238, 307
CviAII CATG 1 cut(s) 139
CviJI RGCY 2 cut(s) 198, 221
CviKI_1 RGCY 2 cut(s) 198, 221
DdeI CTNAG 2 cut(s) 233, 268
DpnI GATC 4 cut(s) 131, 158, 216, 260
DpnII GATC 4 cut(s) 129, 156, 214, 258
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 171
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 1 cut(s) 142
FaiI YATR 1 cut(s) 140
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 1 cut(s) 310
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 2 cut(s) 62, 209
GluI GCNGC 1 cut(s) 316
HapII CCGG 1 cut(s) 218
HgaI GACGC 3 cut(s) 197, 238, 307
Hin1I GRCGYC 2 cut(s) 189, 276
Hin1II CATG 1 cut(s) 142
HinfI GANTC 1 cut(s) 58
HpaII CCGG 1 cut(s) 218
HphI GGTGA 5 cut(s) 16, 89, 100, 253, 274
Hpy188I TCNGA 2 cut(s) 129, 236
Hpy188III TCNNGA 1 cut(s) 62
Hpy99I CGWCG 5 cut(s) 37, 116, 191, 232, 281
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 2 cut(s) 47, 112
HpyCH4IV ACGT 2 cut(s) 178, 276
HpyCH4V TGCA 1 cut(s) 142
HpyF3I CTNAG 2 cut(s) 233, 268
HpySE526I ACGT 2 cut(s) 178, 276
Hsp92I GRCGYC 2 cut(s) 189, 276
Hsp92II CATG 1 cut(s) 142
Kzo9I GATC 4 cut(s) 129, 156, 214, 258
LmnI GCTCC 1 cut(s) 81
LpnPI CCDG 3 cut(s) 86, 231, 289
MaeI CTAG 2 cut(s) 62, 209
MaeII ACGT 2 cut(s) 178, 276
MaeIII GTNAC 2 cut(s) 280, 319
MalI GATC 4 cut(s) 131, 158, 216, 260
MboI GATC 4 cut(s) 129, 156, 214, 258
MboII GAAGA 4 cut(s) 40, 64, 77, 166
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 1 cut(s) 340
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 5 cut(s) 89, 110, 265, 299, 308
MseI TTAA 1 cut(s) 343
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 1 cut(s) 218
MvnI CGCG 1 cut(s) 318
NdeII GATC 4 cut(s) 129, 156, 214, 258
NlaIII CATG 1 cut(s) 142
NmuCI GTSAC 2 cut(s) 280, 319
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PflFI GACNNNGTC 1 cut(s) 278
PkrI GCNGC 1 cut(s) 317
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 156
PsyI GACNNNGTC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 137
SaqAI TTAA 1 cut(s) 343
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 4 cut(s) 129, 156, 214, 258
SchI GAGTC 1 cut(s) 52
SduI GDGCHC 1 cut(s) 200
SetI ASST 7 cut(s) 75, 102, 181, 269, 279, 291, 300
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 1 cut(s) 340
SsiI CCGC 1 cut(s) 316
SspMI CTAG 2 cut(s) 62, 209
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 2 cut(s) 47, 112
TaiI ACGT 2 cut(s) 181, 279
TaqI TCGA 1 cut(s) 227
TasI AATT 1 cut(s) 340
TauI GCSGC 1 cut(s) 318
Tru1I TTAA 1 cut(s) 343
Tru9I TTAA 1 cut(s) 343
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 2 cut(s) 280, 319
Tsp45I GTSAC 2 cut(s) 280, 319
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 1 cut(s) 52
Tth111I GACNNNGTC 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 61
XspI CTAG 2 cut(s) 62, 209
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.