FvH4_6g27231

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002484
Physical Location & Seq
Forward (+)
20903539 .. 20905186
1648 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27231.t1

Sequence Viewer

Length: 534 bp
ATGGCGAGCAAAGCTAAGAACAACTCCTCCAACGCCAAGGTGTACCTGGAAGATTACCTCCTTCTCCTTCACTCCCGCTCCCACCTCAACCTCACCGTCACCCATCTCAACCAGATCATCCACATGCACGGCTTCAAGAAGATCCACAAAGTCCATAAGAAGCTTCTGAGCGACGCCGTGAACTCACTGGACCTGGTGAACCCGTGCCGCTCTACTCTCCACGACTACATCTCGCCGCTGGTGACGGTGAAGACGGAGGACGTCATGGCTGACCTCAGCGACCTCAACTGGCAAGAGTGCCACATCACCTCCATCGAAACCCTCAGCCGTTCATGGAACAACGTGAACCAGTACCAGTCTCTTCCTCCCCGAAACGACGCCGTCAAGTTCCAACAGTACTCGCCGCCCCCCGCTCCGGATTCCGTCGCCCACAGCGTGGTCGTTAAAGCTCCCTTTCCGGCCAAGAGGAGATCGGCGCGGAAGAGGAGGCGCACTGCGAAAGTGGCAGATCTCGTAAATATCTCTTTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.08

Weight (kDa)

9.79

Isoelectric Point (pI)

73.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 11 - 67 3.8e-25 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccB7I CCANNNNNTGG 1 cut(s) 436
AccBSI CCGCTC 3 cut(s) 78, 210, 413
AccII CGCG 1 cut(s) 478
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 6 cut(s) 76, 208, 236, 404, 411, 478
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 459
AcyI GRCGYC 3 cut(s) 174, 261, 378
AdeI CACNNNGTG 1 cut(s) 436
AfaI GTAC 3 cut(s) 44, 353, 398
AfiI CCNNNNNNNGG 2 cut(s) 415, 436
AgsI TTSAA 1 cut(s) 136
AjnI CCWGG 2 cut(s) 45, 192
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 3 cut(s) 14, 163, 449
AluI AGCT 3 cut(s) 14, 163, 449
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 136
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 1 cut(s) 459
AspLEI GCGC 2 cut(s) 478, 492
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 6 cut(s) 85, 91, 208, 253, 259, 298
AvaII GGWCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 257
BbvCI CCTCAGC 2 cut(s) 275, 323
BccI CCATC 2 cut(s) 111, 320
BceAI ACGGC 4 cut(s) 145, 161, 312, 365
BciT130I CCWGG 2 cut(s) 47, 194
BcoDI GTCTC 1 cut(s) 363
BglII AGATCT 1 cut(s) 508
BisI GCNGC 3 cut(s) 208, 236, 404
BlsI GCNGC 3 cut(s) 209, 237, 405
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 47, 194
Bme18I GGWCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 190
BmrFI CCNGG 2 cut(s) 47, 194
BpiI GAAGAC 1 cut(s) 257
Bpu10I CCTNAGC 2 cut(s) 275, 323
BsaHI GRCGYC 3 cut(s) 174, 261, 378
BsaJI CCNNGG 1 cut(s) 36
BsaWI WCCGGW 1 cut(s) 415
BsaXI ACNNNNNCTCC 6 cut(s) 11, 41, 62, 92, 293, 323
Bsc4I CCNNNNNNNGG 2 cut(s) 415, 436
Bse1I ACTGG 4 cut(s) 192, 293, 349, 355
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 2 cut(s) 47, 194
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 415, 436
BseMII CTCAG 3 cut(s) 158, 289, 337
BseNI ACTGG 4 cut(s) 192, 293, 349, 355
BseRI GAGGAG 3 cut(s) 16, 481, 499
Bsh1236I CGCG 1 cut(s) 478
BshFI GGCC 1 cut(s) 461
BsiSI CCGG 2 cut(s) 416, 458
BslI CCNNNNNNNGG 2 cut(s) 415, 436
BsmAI GTCTC 1 cut(s) 363
BsnI GGCC 1 cut(s) 461
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 4 cut(s) 114, 141, 470, 508
BspACI CCGC 6 cut(s) 76, 208, 236, 404, 411, 478
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 3 cut(s) 159, 288, 336
BspEI TCCGGA 1 cut(s) 415
BspFNI CGCG 1 cut(s) 478
BspPI GGATC 1 cut(s) 136
BsrBI CCGCTC 3 cut(s) 78, 210, 413
BsrI ACTGG 4 cut(s) 192, 293, 349, 355
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 4 cut(s) 114, 141, 470, 508
BssNI GRCGYC 3 cut(s) 174, 261, 378
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 2 cut(s) 47, 194
Bst4CI ACNGT 3 cut(s) 97, 247, 396
Bst6I CTCTTC 2 cut(s) 366, 476
BstACI GRCGYC 3 cut(s) 174, 261, 378
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 4 cut(s) 15, 167, 275, 323
BstF5I GGATG 1 cut(s) 117
BstFNI CGCG 1 cut(s) 478
BstHHI GCGC 2 cut(s) 478, 492
BstKTI GATC 4 cut(s) 117, 144, 473, 511
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 4 cut(s) 114, 141, 470, 508
BstMWI GCNNNNNNNGC 2 cut(s) 11, 503
BstNI CCWGG 2 cut(s) 47, 194
BstNSI RCATGY 1 cut(s) 127
BstSCI CCNGG 2 cut(s) 45, 192
BstUI CGCG 1 cut(s) 478
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 2 cut(s) 141, 508
BstYI RGATCY 2 cut(s) 141, 508
BsuRI GGCC 1 cut(s) 461
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 2 cut(s) 185, 492
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 2 cut(s) 478, 492
Cfr13I GGNCC 1 cut(s) 190
CseI GACGC 2 cut(s) 182, 386
CsiI ACCWGGT 1 cut(s) 192
Csp6I GTAC 3 cut(s) 43, 352, 397
CviAII CATG 3 cut(s) 124, 265, 333
CviJI RGCY 7 cut(s) 14, 132, 163, 269, 327, 449, 461
CviKI_1 RGCY 7 cut(s) 14, 132, 163, 269, 327, 449, 461
CviQI GTAC 3 cut(s) 43, 352, 397
DdeI CTNAG 4 cut(s) 15, 167, 275, 323
DpnI GATC 4 cut(s) 116, 143, 472, 510
DpnII GATC 4 cut(s) 114, 141, 470, 508
DraIII CACNNNGTG 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 366, 476
EarI CTCTTC 2 cut(s) 366, 476
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 190
EcoRII CCWGG 2 cut(s) 45, 192
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 3 cut(s) 127, 268, 336
FaiI YATR 4 cut(s) 125, 156, 266, 334
FatI CATG 3 cut(s) 123, 264, 332
FauI CCCGC 2 cut(s) 83, 418
Fnu4HI GCNGC 3 cut(s) 208, 236, 404
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 3 cut(s) 208, 236, 404
GlaI GCGC 2 cut(s) 477, 491
GluI GCNGC 3 cut(s) 208, 236, 404
HaeIII GGCC 1 cut(s) 461
HapII CCGG 2 cut(s) 416, 458
HgaI GACGC 2 cut(s) 182, 386
HhaI GCGC 2 cut(s) 478, 492
Hin1I GRCGYC 3 cut(s) 174, 261, 378
Hin1II CATG 3 cut(s) 127, 268, 336
Hin6I GCGC 2 cut(s) 476, 490
HinP1I GCGC 2 cut(s) 476, 490
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 1 cut(s) 419
HpaII CCGG 2 cut(s) 416, 458
HphI GGTGA 6 cut(s) 85, 91, 208, 253, 259, 298
Hpy166II GTNNAC 4 cut(s) 43, 181, 199, 346
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 2 cut(s) 136, 416
Hpy8I GTNNAC 4 cut(s) 43, 181, 199, 346
Hpy99I CGWCG 3 cut(s) 176, 380, 428
HpyAV CCTTC 2 cut(s) 71, 77
HpyCH4III ACNGT 3 cut(s) 97, 247, 396
HpyCH4IV ACGT 2 cut(s) 261, 342
HpyCH4V TGCA 1 cut(s) 127
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 503
HpyF3I CTNAG 4 cut(s) 15, 167, 275, 323
HpySE526I ACGT 2 cut(s) 261, 342
Hsp92I GRCGYC 3 cut(s) 174, 261, 378
Hsp92II CATG 3 cut(s) 127, 268, 336
HspAI GCGC 2 cut(s) 476, 490
Kpn2I TCCGGA 1 cut(s) 415
Kzo9I GATC 4 cut(s) 114, 141, 470, 508
LmnI GCTCC 3 cut(s) 83, 418, 454
MabI ACCWGGT 1 cut(s) 192
MaeII ACGT 2 cut(s) 261, 342
MaeIII GTNAC 2 cut(s) 97, 241
MalI GATC 4 cut(s) 116, 143, 472, 510
MbiI CCGCTC 3 cut(s) 78, 210, 413
MboI GATC 4 cut(s) 114, 141, 470, 508
MboII GAAGA 5 cut(s) 62, 151, 262, 353, 493
MflI RGATCY 2 cut(s) 141, 508
MmeI TCCRAC 2 cut(s) 54, 415
MroI TCCGGA 1 cut(s) 415
MseI TTAA 1 cut(s) 444
MslI CAYNNNNRTG 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 238
MspI CCGG 2 cut(s) 416, 458
MspR9I CCNGG 2 cut(s) 47, 194
MvaI CCWGG 2 cut(s) 47, 194
MvnI CGCG 1 cut(s) 478
MwoI GCNNNNNNNGC 2 cut(s) 11, 503
NdeII GATC 4 cut(s) 114, 141, 470, 508
NlaIII CATG 3 cut(s) 127, 268, 336
NmuCI GTSAC 2 cut(s) 97, 241
NspI RCATGY 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PfeI GAWTC 1 cut(s) 419
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 436
PkrI GCNGC 3 cut(s) 209, 237, 405
Psp6I CCWGG 2 cut(s) 45, 192
PspGI CCWGG 2 cut(s) 45, 192
PspPI GGNCC 1 cut(s) 190
PsuI RGATCY 2 cut(s) 141, 508
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 3 cut(s) 44, 353, 398
RsaNI GTAC 3 cut(s) 43, 352, 397
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 444
SatI GCNGC 3 cut(s) 208, 236, 404
Sau3AI GATC 4 cut(s) 114, 141, 470, 508
Sau96I GGNCC 1 cut(s) 190
ScaI AGTACT 1 cut(s) 398
ScrFI CCNGG 2 cut(s) 47, 194
SexAI ACCWGGT 1 cut(s) 192
SinI GGWCC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 122
SsiI CCGC 6 cut(s) 76, 208, 236, 404, 411, 478
StyD4I CCNGG 2 cut(s) 45, 192
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 3 cut(s) 97, 247, 396
TaiI ACGT 2 cut(s) 264, 345
TaqI TCGA 1 cut(s) 315
TatI WGTACW 1 cut(s) 396
TauI GCSGC 3 cut(s) 210, 238, 406
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 1 cut(s) 444
Tru9I TTAA 1 cut(s) 444
TscAI CASTG 2 cut(s) 192, 499
TseFI GTSAC 2 cut(s) 97, 241
Tsp45I GTSAC 2 cut(s) 97, 241
TspDTI ATGAA 1 cut(s) 321
TspGWI ACGGA 2 cut(s) 269, 412
TspRI CASTG 2 cut(s) 192, 499
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 190
XceI RCATGY 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
ZraI GACGTC 1 cut(s) 262
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.