Rorug02G0286100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002484
Physical Location & Seq
Forward (+)
31165180 .. 31166361
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0286100.1

Sequence Viewer

Length: 1182 bp
ATGGATTACAAAGATCTCGAAGCTCTCCGAAAACCCATCAGCAACCAAACCGATGTGGCACTGAAGATAACAAAGCAACTCCTTGAATCTGAATTCAAGGACAAGAACCTGATCTTCTCCCCTCTATCCATCCACATCGTCCTGAGCTTAATAGCCGCCCGGACAAACAATCCCCAGTTTCTCTCTTTTCTCAAGTCCAAGTCCATCGACGACCTCAATTCCCTCGCCTTCAATCTCATCACTTCCGTTTTGGCCGACTCACCCAGCAGAGGCGGGCCACGTTTGAACTCTACAAACGGTCTCTGGGTCGACGAGTCTATTCCTCTGGAGGACTCCTACAAACAAGTCGTGTTGGATTCTTACAAGGCGGCTCTAAATCAAGTCGATTTCAAAACTGATCCGGAACAAGTGAGAATCCAAGTGAACTCATGGGTTGATAAAGAGACCAAAGGCCTTATCCCTGAGATTCTTCCTCCTGGCTCAGTCAATAGCGGGGTGTGTCTCATATTTGCAAATGCCTTGTACTTCAAAGCAACTTGGGACGACGATTTCTTCTATGAACCACCGAACTCGAAAGACCATAAGTTTCATCTTCTGAACGGGGACTCGGTTAAGGGAGTGCCTTTCATGACTAGTAGTCACTGCCACTCAATCGCTGCCTTTGACGGCTTTAAAGTCTTGAGTCTTCCTTACAGAGATTGTGCTTTAGAGGATTTTGACAATGGCACATATGCAGACGACCCCCGATCTTTCTCTATGTTGTGGTTACTTCCGGATGCAAGAGATGGGTTGCCGACTCTGACTGAGAGAGTTTGTTCCGAGTCCGGGTTTCTAGACAGCCATCTTAATAGTTTTACGACTGTTAGAGTGAAGAAATTCTTGATTCCAAAGTTTAAGATTTCATCCGGGTTTGAAGCTTCGGGTGTTCTGAAGAAAATAGGGCTGGGGGATGGTTGTGATTTAATGGCGATATTTCACGAATCTGTAATAGACGTTGATGAAAATGGCACAACAGCTGCAGCTGCTACTTGTGCTCAAATGTGGGATTGCGATGGTGACTTCTCCAAACCTAAAGAGATAGAAGAATTTGGGGCTGACCACCCCTTCATGTTTCTCATAAGGGAAAACAGAACCGGAACGGTGCTGTTCATGGGGCAGGTGCTCAATCCTCTTGCAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

43.63

Weight (kDa)

4.94

Isoelectric Point (pI)

26.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serpin PF00079 15 - 390 2.1e-70 Serpin (serine protease inhibitor)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1147
Acc36I ACCTGC 1 cut(s) 1147
AccI GTMKAC 1 cut(s) 309
AccIII TCCGGA 2 cut(s) 400, 772
AciI CCGC 4 cut(s) 156, 273, 368, 492
AclWI GGATC 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 3 cut(s) 92, 875, 1085
AcuI CTGAAG 2 cut(s) 83, 950
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 3 cut(s) 269, 825, 1175
AgsI TTSAA 7 cut(s) 86, 97, 232, 286, 391, 529, 914
AhdI GACNNNNNGTC 1 cut(s) 636
AhlI ACTAGT 1 cut(s) 632
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 5 cut(s) 23, 147, 917, 1016, 1022
AluI AGCT 5 cut(s) 23, 147, 917, 1016, 1022
Alw21I GWGCWC 2 cut(s) 1036, 1164
Alw26I GTCTC 3 cut(s) 305, 437, 506
AlwI GGATC 1 cut(s) 392
Aor13HI TCCGGA 2 cut(s) 400, 772
AoxI GGCC 3 cut(s) 252, 275, 451
ApeKI GCWGC 4 cut(s) 656, 1016, 1019, 1022
ApoI RAATTY 3 cut(s) 92, 875, 1085
Asp700I GAANNNNTTC 1 cut(s) 875
AspS9I GGNCC 1 cut(s) 275
AsuC2I CCSGG 3 cut(s) 160, 826, 907
AsuHPI GGTGA 2 cut(s) 252, 1067
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 2 cut(s) 1036, 1164
BbvI GCAGC 4 cut(s) 643, 1003, 1009, 1031
BccI CCATC 7 cut(s) 44, 137, 212, 779, 849, 944, 1046
BceAI ACGGC 1 cut(s) 682
BciT130I CCWGG 1 cut(s) 477
BcnI CCSGG 3 cut(s) 160, 826, 907
BcoDI GTCTC 3 cut(s) 305, 437, 506
BcuI ACTAGT 1 cut(s) 632
BfaI CTAG 2 cut(s) 633, 833
BfmI CTRYAG 1 cut(s) 1017
BfuAI ACCTGC 1 cut(s) 1147
BglII AGATCT 1 cut(s) 13
BisI GCNGC 6 cut(s) 156, 369, 657, 1017, 1020, 1023
BlsI GCNGC 6 cut(s) 157, 370, 658, 1018, 1021, 1024
Bme1390I CCNGG 4 cut(s) 160, 477, 826, 907
BmeRI GACNNNNNGTC 1 cut(s) 636
BmgT120I GGNCC 1 cut(s) 275
BmrFI CCNGG 4 cut(s) 160, 477, 826, 907
BmrI ACTGGG 1 cut(s) 169
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 169
BpiI GAAGAC 1 cut(s) 677
BpmI CTGGAG 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 143
BpuEI CTTGAG 2 cut(s) 176, 700
BpuMI CCSGG 3 cut(s) 160, 826, 907
BsaI GGTCTC 2 cut(s) 305, 437
BsaWI WCCGGW 3 cut(s) 400, 772, 1133
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 825, 1175
Bse1I ACTGG 1 cut(s) 175
BseAI TCCGGA 2 cut(s) 400, 772
BseBI CCWGG 1 cut(s) 477
BseGI GGATG 4 cut(s) 129, 781, 902, 955
BseLI CCNNNNNNNGG 3 cut(s) 269, 825, 1175
BseMII CTCAG 4 cut(s) 134, 453, 495, 795
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 4 cut(s) 643, 1003, 1009, 1031
BseYI CCCAGC 2 cut(s) 263, 943
BshFI GGCC 3 cut(s) 254, 277, 453
BsiHKAI GWGCWC 2 cut(s) 1036, 1164
BsiSI CCGG 6 cut(s) 160, 401, 773, 825, 906, 1134
BslFI GGGAC 2 cut(s) 554, 617
BslI CCNNNNNNNGG 3 cut(s) 269, 825, 1175
BsmAI GTCTC 3 cut(s) 305, 437, 506
BsmFI GGGAC 2 cut(s) 554, 617
BsnI GGCC 3 cut(s) 254, 277, 453
Bso31I GGTCTC 2 cut(s) 305, 437
Bsp1286I GDGCHC 2 cut(s) 1036, 1164
Bsp13I TCCGGA 2 cut(s) 400, 772
Bsp143I GATC 4 cut(s) 13, 111, 397, 746
BspACI CCGC 4 cut(s) 156, 273, 368, 492
BspANI GGCC 3 cut(s) 254, 277, 453
BspCNI CTCAG 4 cut(s) 135, 454, 494, 796
BspEI TCCGGA 2 cut(s) 400, 772
BspHI TCATGA 1 cut(s) 627
BspMAI CTGCAG 1 cut(s) 1021
BspMI ACCTGC 1 cut(s) 1147
BspPI GGATC 1 cut(s) 392
BspTNI GGTCTC 2 cut(s) 305, 437
BsrI ACTGG 1 cut(s) 175
BssMI GATC 4 cut(s) 13, 111, 397, 746
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 3 cut(s) 299, 862, 1141
BstC8I GCNNGC 2 cut(s) 275, 1177
BstDEI CTNAG 4 cut(s) 143, 462, 481, 804
BstENI CCTNNNNNAGG 1 cut(s) 1173
BstF5I GGATG 4 cut(s) 129, 781, 902, 955
BstKTI GATC 4 cut(s) 16, 114, 400, 749
BstMAI GTCTC 3 cut(s) 305, 437, 506
BstMBI GATC 4 cut(s) 13, 111, 397, 746
BstMWI GCNNNNNNNGC 2 cut(s) 1022, 1031
BstNI CCWGG 1 cut(s) 477
BstSCI CCNGG 4 cut(s) 158, 475, 824, 905
BstSFI CTRYAG 1 cut(s) 1017
BstV1I GCAGC 4 cut(s) 643, 1003, 1009, 1031
BstV2I GAAGAC 1 cut(s) 677
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 3 cut(s) 254, 277, 453
BtgZI GCGATG 1 cut(s) 1065
BtsCI GGATG 4 cut(s) 129, 781, 902, 955
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 2 cut(s) 59, 640
BveI ACCTGC 1 cut(s) 1147
Cac8I GCNNGC 2 cut(s) 275, 1177
CciI TCATGA 1 cut(s) 627
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 523
CviAII CATG 4 cut(s) 429, 628, 1108, 1150
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 4 cut(s) 143, 462, 481, 804
DpnI GATC 4 cut(s) 15, 113, 399, 748
DpnII GATC 4 cut(s) 13, 111, 397, 746
DraI TTTAAA 1 cut(s) 673
DriI GACNNNNNGTC 1 cut(s) 636
EaeI YGGCCR 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 636
Eco147I AGGCCT 1 cut(s) 453
Eco31I GGTCTC 2 cut(s) 305, 437
Eco57I CTGAAG 2 cut(s) 83, 950
EcoNI CCTNNNNNAGG 1 cut(s) 1173
EcoRI GAATTC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 475
FaeI CATG 4 cut(s) 432, 631, 1111, 1153
FalI AAGNNNNNCTT 2 cut(s) 863, 895
FaqI GGGAC 2 cut(s) 554, 617
FatI CATG 4 cut(s) 428, 627, 1107, 1149
FauI CCCGC 2 cut(s) 266, 485
FauNDI CATATG 1 cut(s) 730
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 6 cut(s) 156, 369, 657, 1017, 1020, 1023
FokI GGATG 4 cut(s) 116, 788, 889, 962
Fsp4HI GCNGC 6 cut(s) 156, 369, 657, 1017, 1020, 1023
FspBI CTAG 2 cut(s) 633, 833
GluI GCNGC 6 cut(s) 156, 369, 657, 1017, 1020, 1023
GsaI CCCAGC 2 cut(s) 267, 947
GsuI CTGGAG 1 cut(s) 347
HaeIII GGCC 3 cut(s) 254, 277, 453
HapII CCGG 6 cut(s) 160, 401, 773, 825, 906, 1134
Hin1II CATG 4 cut(s) 432, 631, 1111, 1153
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 1 cut(s) 915
HpaII CCGG 6 cut(s) 160, 401, 773, 825, 906, 1134
HphI GGTGA 2 cut(s) 252, 1067
Hpy166II GTNNAC 2 cut(s) 310, 424
Hpy188I TCNGA 6 cut(s) 29, 91, 597, 801, 820, 930
Hpy8I GTNNAC 2 cut(s) 310, 424
Hpy99I CGWCG 3 cut(s) 212, 314, 548
HpyAV CCTTC 2 cut(s) 238, 1114
HpyCH4III ACNGT 3 cut(s) 299, 862, 1141
HpyCH4IV ACGT 2 cut(s) 280, 993
HpyCH4V TGCA 5 cut(s) 512, 734, 779, 1019, 1175
HpyF10VI GCNNNNNNNGC 2 cut(s) 1022, 1031
HpyF3I CTNAG 4 cut(s) 143, 462, 481, 804
HpySE526I ACGT 2 cut(s) 280, 993
Hsp92II CATG 4 cut(s) 432, 631, 1111, 1153
Kpn2I TCCGGA 2 cut(s) 400, 772
Kzo9I GATC 4 cut(s) 13, 111, 397, 746
Lsp1109I GCAGC 4 cut(s) 643, 1003, 1009, 1031
LweI GCATC 1 cut(s) 766
MaeI CTAG 2 cut(s) 633, 833
MaeII ACGT 2 cut(s) 280, 993
MaeIII GTNAC 3 cut(s) 638, 765, 1055
MalI GATC 4 cut(s) 15, 113, 399, 748
MboI GATC 4 cut(s) 13, 111, 397, 746
MboII GAAGA 9 cut(s) 76, 106, 461, 544, 584, 677, 883, 943, 1094
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 1036, 1164
MluCI AATT 4 cut(s) 92, 217, 875, 1085
MlyI GAGTC 7 cut(s) 251, 323, 326, 599, 691, 790, 830
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 9 cut(s) 132, 224, 233, 263, 322, 333, 483, 703, 1179
MroI TCCGGA 2 cut(s) 400, 772
MroXI GAANNNNTTC 1 cut(s) 875
MseI TTAA 6 cut(s) 149, 612, 672, 846, 894, 962
MspA1I CMGCKG 2 cut(s) 1016, 1022
MspI CCGG 6 cut(s) 160, 401, 773, 825, 906, 1134
MspR9I CCNGG 4 cut(s) 160, 477, 826, 907
MvaI CCWGG 1 cut(s) 477
MwoI GCNNNNNNNGC 2 cut(s) 1022, 1031
NciI CCSGG 3 cut(s) 160, 826, 907
NdeI CATATG 1 cut(s) 730
NdeII GATC 4 cut(s) 13, 111, 397, 746
NlaIII CATG 4 cut(s) 432, 631, 1111, 1153
NmuCI GTSAC 2 cut(s) 638, 1055
PagI TCATGA 1 cut(s) 627
PaqCI CACCTGC 1 cut(s) 1147
PceI AGGCCT 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 875
PfeI GAWTC 6 cut(s) 86, 356, 414, 466, 883, 980
PkrI GCNGC 6 cut(s) 157, 370, 658, 1018, 1021, 1024
PleI GAGTC 7 cut(s) 251, 322, 326, 599, 690, 790, 829
PpsI GAGTC 7 cut(s) 251, 322, 326, 599, 690, 790, 829
Psp6I CCWGG 1 cut(s) 475
PspFI CCCAGC 2 cut(s) 263, 943
PspGI CCWGG 1 cut(s) 475
PspPI GGNCC 1 cut(s) 275
PstI CTGCAG 1 cut(s) 1021
PsuI RGATCY 1 cut(s) 13
PvuII CAGCTG 2 cut(s) 1016, 1022
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 6 cut(s) 149, 612, 672, 846, 894, 962
SatI GCNGC 6 cut(s) 156, 369, 657, 1017, 1020, 1023
Sau3AI GATC 4 cut(s) 13, 111, 397, 746
Sau96I GGNCC 1 cut(s) 275
SchI GAGTC 7 cut(s) 251, 323, 326, 599, 691, 790, 830
ScrFI CCNGG 4 cut(s) 160, 477, 826, 907
SduI GDGCHC 2 cut(s) 1036, 1164
SfaNI GCATC 1 cut(s) 766
SfcI CTRYAG 1 cut(s) 1017
SmlI CTYRAG 2 cut(s) 191, 679
SmoI CTYRAG 2 cut(s) 191, 679
SpeI ACTAGT 1 cut(s) 632
Sse9I AATT 4 cut(s) 92, 217, 875, 1085
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 4 cut(s) 156, 273, 368, 492
SspMI CTAG 2 cut(s) 633, 833
StuI AGGCCT 1 cut(s) 453
StyD4I CCNGG 4 cut(s) 158, 475, 824, 905
TaaI ACNGT 3 cut(s) 299, 862, 1141
TaiI ACGT 2 cut(s) 283, 996
TaqI TCGA 5 cut(s) 18, 207, 309, 384, 572
TasI AATT 4 cut(s) 92, 217, 875, 1085
TatI WGTACW 1 cut(s) 522
TauI GCSGC 2 cut(s) 158, 371
TfiI GAWTC 6 cut(s) 86, 356, 414, 466, 883, 980
Tru1I TTAA 6 cut(s) 149, 612, 672, 846, 894, 962
Tru9I TTAA 6 cut(s) 149, 612, 672, 846, 894, 962
TscAI CASTG 2 cut(s) 66, 647
TseFI GTSAC 2 cut(s) 638, 1055
TseI GCWGC 4 cut(s) 656, 1016, 1019, 1022
Tsp45I GTSAC 2 cut(s) 638, 1055
TspDTI ATGAA 7 cut(s) 573, 578, 616, 891, 1014, 1096, 1138
TspGWI ACGGA 1 cut(s) 235
TspRI CASTG 2 cut(s) 66, 647
XagI CCTNNNNNAGG 1 cut(s) 1173
XapI RAATTY 3 cut(s) 92, 875, 1085
XbaI TCTAGA 1 cut(s) 832
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 2 cut(s) 633, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.