Rh2DG364000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002484
Physical Location & Seq
Forward (+)
51362074 .. 51379222
17149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG364000.1

Sequence Viewer

Length: 510 bp
ATGCTGATGGAGATGGCGAGGAAAGCTAAGAATAACGCGAATGCGAAGATGTCTCTGGATGATTACCTCCTTCTCCTCCAGTCTGGCTCCCACCTCCACCTCACCGTCAGCCATCTCAATCAGATTATCAGCATGCACGGCTACAAGAAGATCCACAAAGTTCACAAGAAGCTTCTGAGCGACGCCGTGAACTCACTGCAGCTGGTGAACCCGTGCCGCTCCACGCTTAGGGACTACATCTCGCCGTTTGTGACGACGAAGATGGATGACGTCTTCGCCGACCTCAGCGATCTCAACTGGCAAGACTGTTGCATCACCTCCATCCAAACCCTCAGTTCGTGGGAGGACCAGTCCCCTCCTCCCCGAAACGTCGCCGTCCAGTTCCAACACTACTCGCCGTCCACCGCAGCCCATAGCGTGATCGTTTCTGCTCCCGGACCGACCAAGAAGAGATCCGCGCGGAAGAGGAAGCGGACTGCTAAAGTGGCCGATCTGGTAAATTTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.97

Weight (kDa)

9.65

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 14 - 70 3.6e-24 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 219
AccII CGCG 3 cut(s) 38, 458, 460
AciI CCGC 5 cut(s) 217, 405, 456, 460, 472
AclWI GGATC 2 cut(s) 145, 447
AcoI YGGCCR 1 cut(s) 486
AcsI RAATTY 1 cut(s) 499
AcyI GRCGYC 2 cut(s) 183, 270
AfiI CCNNNNNNNGG 1 cut(s) 228
AluBI AGCT 3 cut(s) 26, 172, 202
AluI AGCT 3 cut(s) 26, 172, 202
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 2 cut(s) 145, 447
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 2 cut(s) 199, 407
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 2 cut(s) 346, 437
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 3 cut(s) 94, 217, 307
AvaII GGWCC 2 cut(s) 346, 437
BbsI GAAGAC 1 cut(s) 265
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 211, 419
BccI CCATC 4 cut(s) 7, 120, 256, 329
BceAI ACGGC 5 cut(s) 154, 170, 229, 359, 382
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 197
BisI GCNGC 3 cut(s) 200, 217, 408
BlsI GCNGC 3 cut(s) 201, 218, 409
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 346, 437
BmgT120I GGNCC 2 cut(s) 346, 437
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 435
BmsI GCATC 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 265
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 2 cut(s) 227, 284
BpuMI CCSGG 1 cut(s) 435
BsaHI GRCGYC 2 cut(s) 183, 270
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse1I ACTGG 4 cut(s) 79, 302, 349, 379
BseGI GGATG 3 cut(s) 64, 271, 321
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMII CTCAG 3 cut(s) 167, 298, 346
BseNI ACTGG 4 cut(s) 79, 302, 349, 379
BseRI GAGGAG 2 cut(s) 65, 348
BseXI GCAGC 2 cut(s) 211, 419
Bsh1236I CGCG 3 cut(s) 38, 458, 460
BshFI GGCC 1 cut(s) 488
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 2 cut(s) 245, 337
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 245, 337
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 1 cut(s) 488
Bsp143I GATC 5 cut(s) 150, 289, 420, 452, 490
BspACI CCGC 5 cut(s) 217, 405, 456, 460, 472
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 3 cut(s) 168, 297, 345
BspFNI CGCG 3 cut(s) 38, 458, 460
BspLI GGNNCC 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 2 cut(s) 145, 447
BsrBI CCGCTC 1 cut(s) 219
BsrI ACTGG 4 cut(s) 79, 302, 349, 379
BssMI GATC 5 cut(s) 150, 289, 420, 452, 490
BssNI GRCGYC 2 cut(s) 183, 270
Bst4CI ACNGT 2 cut(s) 106, 308
Bst6I CTCTTC 2 cut(s) 443, 458
BstACI GRCGYC 2 cut(s) 183, 270
BstC8I GCNNGC 1 cut(s) 134
BstDEI CTNAG 5 cut(s) 27, 176, 227, 284, 332
BstF5I GGATG 3 cut(s) 64, 271, 321
BstFNI CGCG 3 cut(s) 38, 458, 460
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 5 cut(s) 153, 292, 423, 455, 493
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 5 cut(s) 150, 289, 420, 452, 490
BstMWI GCNNNNNNNGC 3 cut(s) 23, 138, 485
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 197
BstUI CGCG 3 cut(s) 38, 458, 460
BstV1I GCAGC 2 cut(s) 211, 419
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 2 cut(s) 150, 452
BstYI RGATCY 2 cut(s) 150, 452
BsuRI GGCC 1 cut(s) 488
BtsCI GGATG 3 cut(s) 64, 271, 321
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 134
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 2 cut(s) 346, 437
CpoI CGGWCCG 1 cut(s) 437
CseI GACGC 1 cut(s) 191
CspI CGGWCCG 1 cut(s) 437
CviAII CATG 1 cut(s) 133
CviJI RGCY 8 cut(s) 26, 87, 111, 141, 172, 202, 410, 488
CviKI_1 RGCY 8 cut(s) 26, 87, 111, 141, 172, 202, 410, 488
DdeI CTNAG 5 cut(s) 27, 176, 227, 284, 332
DpnI GATC 5 cut(s) 152, 291, 422, 454, 492
DpnII GATC 5 cut(s) 150, 289, 420, 452, 490
EaeI YGGCCR 1 cut(s) 486
Eam1104I CTCTTC 2 cut(s) 443, 458
EarI CTCTTC 2 cut(s) 443, 458
Eco47I GGWCC 2 cut(s) 346, 437
FaeI CATG 1 cut(s) 136
FaiI YATR 2 cut(s) 134, 414
FaqI GGGAC 2 cut(s) 245, 337
FatI CATG 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 200, 217, 408
FokI GGATG 3 cut(s) 71, 278, 308
Fsp4HI GCNGC 3 cut(s) 200, 217, 408
GlaI GCGC 1 cut(s) 459
GluI GCNGC 3 cut(s) 200, 217, 408
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 1 cut(s) 488
HapII CCGG 1 cut(s) 435
HgaI GACGC 1 cut(s) 191
HhaI GCGC 1 cut(s) 460
Hin1I GRCGYC 2 cut(s) 183, 270
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HindIII AAGCTT 1 cut(s) 170
HpaII CCGG 1 cut(s) 435
HphI GGTGA 3 cut(s) 94, 217, 307
Hpy166II GTNNAC 4 cut(s) 163, 190, 208, 402
Hpy188I TCNGA 2 cut(s) 123, 177
Hpy188III TCNNGA 1 cut(s) 56
Hpy8I GTNNAC 4 cut(s) 163, 190, 208, 402
Hpy99I CGWCG 3 cut(s) 185, 259, 374
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 2 cut(s) 106, 308
HpyCH4IV ACGT 2 cut(s) 270, 369
HpyCH4V TGCA 3 cut(s) 136, 199, 312
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 138, 485
HpyF3I CTNAG 5 cut(s) 27, 176, 227, 284, 332
HpySE526I ACGT 2 cut(s) 270, 369
Hsp92I GRCGYC 2 cut(s) 183, 270
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 1 cut(s) 458
Kzo9I GATC 5 cut(s) 150, 289, 420, 452, 490
LmnI GCTCC 3 cut(s) 92, 224, 436
LpnPI CCDG 9 cut(s) 41, 69, 92, 188, 283, 362, 392, 448, 479
Lsp1109I GCAGC 2 cut(s) 211, 419
LweI GCATC 1 cut(s) 321
MaeII ACGT 2 cut(s) 270, 369
MaeIII GTNAC 1 cut(s) 250
MalI GATC 5 cut(s) 152, 291, 422, 454, 492
MbiI CCGCTC 1 cut(s) 219
MboI GATC 5 cut(s) 150, 289, 420, 452, 490
MboII GAAGA 6 cut(s) 58, 160, 265, 271, 460, 475
MflI RGATCY 2 cut(s) 150, 452
MluCI AATT 1 cut(s) 499
MmeI TCCRAC 1 cut(s) 409
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 1 cut(s) 435
Mva1269I GAATGC 1 cut(s) 46
MvnI CGCG 3 cut(s) 38, 458, 460
MwoI GCNNNNNNNGC 3 cut(s) 23, 138, 485
NciI CCSGG 1 cut(s) 435
NdeII GATC 5 cut(s) 150, 289, 420, 452, 490
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 136
PaeI GCATGC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 433
PkrI GCNGC 3 cut(s) 201, 218, 409
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 2 cut(s) 346, 437
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 2 cut(s) 150, 452
PvuII CAGCTG 1 cut(s) 202
Rsr2I CGGWCCG 1 cut(s) 437
RsrII CGGWCCG 1 cut(s) 437
SatI GCNGC 3 cut(s) 200, 217, 408
Sau3AI GATC 5 cut(s) 150, 289, 420, 452, 490
Sau96I GGNCC 2 cut(s) 346, 437
ScrFI CCNGG 1 cut(s) 435
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 197
SinI GGWCC 2 cut(s) 346, 437
SphI GCATGC 1 cut(s) 136
Sse9I AATT 1 cut(s) 499
SsiI CCGC 5 cut(s) 217, 405, 456, 460, 472
StyD4I CCNGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 106, 308
TaiI ACGT 2 cut(s) 273, 372
TaqII GACCGA 1 cut(s) 454
TasI AATT 1 cut(s) 499
TauI GCSGC 1 cut(s) 219
TscAI CASTG 1 cut(s) 201
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 199, 407
Tsp45I GTSAC 1 cut(s) 250
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 2 cut(s) 346, 437
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 136
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.