Rw2G027420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002484
Physical Location & Seq
Forward (+)
42923150 .. 42923849
700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027420.1

Sequence Viewer

Length: 525 bp
ATGGAGATGGCGAGCAAAGCTAAGAATAACGCGAATGCCAAGATGTCTCTGGATGATTACCTCCTCCTAATCCAGTCTCGCTCCCACCTCCACCTCACCGTCAGCCATCTCGATCAGATCATCAGCATTCACGGCTACAAGAAGATCCACAAAGTTCAAAAGAAGCTTCTAAGCGACACCGTGAACTCACTGCAGCTGGTGAACCCGTGCCGCTCCACGCTTAGGGAGTACTATATCTCGCCGTTGGTGACGACAAAGATGGAGGACGTCTTCGCCGACCTCAGCGATCTCAACTGGCAAGACTATTGCATCACGTTCGTCCAAACCCTCAGTTCGTGGGAGGACCGGTCCCCTCCTCACCGAAACGGCGCCGTCCAGTTCCAACACTACTCGCCGTCCACCGCTCCAGACTCGGCAGCCCACATCGTGATGGTTTCTGCTCCCGCACCGGTCAAGAGGAGATCGGTGCGGAAGAGGAAGCGGACCGCTAAAGTGGCAGATCTGGTCAATTTCACCAAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.76

Weight (kDa)

9.66

Isoelectric Point (pI)

62.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 12 - 68 2.3e-22 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 368
AccBSI CCGCTC 2 cut(s) 213, 404
AccII CGCG 1 cut(s) 32
AciI CCGC 6 cut(s) 211, 402, 444, 469, 481, 486
AclWI GGATC 1 cut(s) 139
AcyI GRCGYC 2 cut(s) 267, 369
AdeI CACNNNGTG 1 cut(s) 427
AfaI GTAC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 222
AgeI ACCGGT 2 cut(s) 345, 448
AgsI TTSAA 1 cut(s) 158
AluBI AGCT 3 cut(s) 20, 166, 196
AluI AGCT 3 cut(s) 20, 166, 196
Alw26I GTCTC 2 cut(s) 51, 81
AlwI GGATC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 193, 416
AsiGI ACCGGT 2 cut(s) 345, 448
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 3 cut(s) 343, 348, 483
AsuHPI GGTGA 5 cut(s) 88, 211, 259, 350, 505
AvaII GGWCC 3 cut(s) 343, 348, 483
BanI GGYRCC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 262
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 205, 428
BccI CCATC 3 cut(s) 114, 253, 424
BceAI ACGGC 5 cut(s) 148, 226, 356, 379, 382
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BcoDI GTCTC 2 cut(s) 51, 81
BfaI CTAG 1 cut(s) 523
BfmI CTRYAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 372
BglII AGATCT 1 cut(s) 499
BisI GCNGC 3 cut(s) 194, 211, 417
BlsI GCNGC 3 cut(s) 195, 212, 418
BmcAI AGTACT 1 cut(s) 230
Bme18I GGWCC 3 cut(s) 343, 348, 483
BmgT120I GGNCC 3 cut(s) 343, 348, 483
BmiI GGNNCC 2 cut(s) 350, 370
BmsI GCATC 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 262
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 2 cut(s) 221, 281
BsaHI GRCGYC 2 cut(s) 267, 369
BsaWI WCCGGW 2 cut(s) 345, 448
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse118I RCCGGY 2 cut(s) 345, 448
Bse1I ACTGG 3 cut(s) 73, 299, 376
BseGI GGATG 1 cut(s) 58
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 295, 343
BseNI ACTGG 3 cut(s) 73, 299, 376
BseRI GAGGAG 3 cut(s) 53, 345, 472
BseXI GCAGC 2 cut(s) 205, 428
Bsh1236I CGCG 1 cut(s) 32
BshNI GGYRCC 1 cut(s) 368
BshTI ACCGGT 2 cut(s) 345, 448
BsiSI CCGG 2 cut(s) 346, 449
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 2 cut(s) 51, 81
BsmFI GGGAC 1 cut(s) 334
BsmI GAATGC 2 cut(s) 40, 126
Bsp143I GATC 6 cut(s) 112, 117, 144, 286, 461, 499
BspACI CCGC 6 cut(s) 211, 402, 444, 469, 481, 486
BspCNI CTCAG 2 cut(s) 294, 342
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 350, 370
BspMAI CTGCAG 1 cut(s) 195
BspPI GGATC 1 cut(s) 139
BspT107I GGYRCC 1 cut(s) 368
BsrBI CCGCTC 2 cut(s) 213, 404
BsrFI RCCGGY 2 cut(s) 345, 448
BsrI ACTGG 3 cut(s) 73, 299, 376
BssAI RCCGGY 2 cut(s) 345, 448
BssMI GATC 6 cut(s) 112, 117, 144, 286, 461, 499
BssNI GRCGYC 2 cut(s) 267, 369
Bst4CI ACNGT 2 cut(s) 100, 181
Bst6I CTCTTC 1 cut(s) 467
BstACI GRCGYC 2 cut(s) 267, 369
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 5 cut(s) 21, 170, 221, 281, 329
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 32
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 6 cut(s) 115, 120, 147, 289, 464, 502
BstMAI GTCTC 2 cut(s) 51, 81
BstMBI GATC 6 cut(s) 112, 117, 144, 286, 461, 499
BstMWI GCNNNNNNNGC 3 cut(s) 17, 132, 494
BstSFI CTRYAG 1 cut(s) 191
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 2 cut(s) 205, 428
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 2 cut(s) 144, 499
BstYI RGATCY 2 cut(s) 144, 499
BtsCI GGATG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 1 cut(s) 188
Cac8I GCNNGC 1 cut(s) 13
CfoI GCGC 1 cut(s) 371
Cfr10I RCCGGY 2 cut(s) 345, 448
Cfr13I GGNCC 3 cut(s) 343, 348, 483
CpoI CGGWCCG 1 cut(s) 483
Csp6I GTAC 1 cut(s) 229
CspAI ACCGGT 2 cut(s) 345, 448
CspI CGGWCCG 1 cut(s) 483
CviJI RGCY 7 cut(s) 20, 105, 135, 166, 196, 419, 521
CviKI_1 RGCY 7 cut(s) 20, 105, 135, 166, 196, 419, 521
CviQI GTAC 1 cut(s) 229
DdeI CTNAG 5 cut(s) 21, 170, 221, 281, 329
DinI GGCGCC 1 cut(s) 370
DpnI GATC 6 cut(s) 114, 119, 146, 288, 463, 501
DpnII GATC 6 cut(s) 112, 117, 144, 286, 461, 499
DraIII CACNNNGTG 1 cut(s) 427
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco47I GGWCC 3 cut(s) 343, 348, 483
EgeI GGCGCC 1 cut(s) 370
EheI GGCGCC 1 cut(s) 370
FaiI YATR 1 cut(s) 234
FaqI GGGAC 1 cut(s) 334
FauI CCCGC 1 cut(s) 451
Fnu4HI GCNGC 3 cut(s) 194, 211, 417
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 3 cut(s) 194, 211, 417
FspBI CTAG 1 cut(s) 523
GlaI GCGC 1 cut(s) 370
GluI GCNGC 3 cut(s) 194, 211, 417
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 372
HapII CCGG 2 cut(s) 346, 449
HhaI GCGC 1 cut(s) 371
Hin1I GRCGYC 2 cut(s) 267, 369
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 1 cut(s) 410
HpaII CCGG 2 cut(s) 346, 449
HphI GGTGA 5 cut(s) 88, 211, 259, 350, 505
Hpy166II GTNNAC 3 cut(s) 184, 202, 399
Hpy188I TCNGA 1 cut(s) 117
Hpy188III TCNNGA 5 cut(s) 50, 110, 407, 427, 454
Hpy8I GTNNAC 3 cut(s) 184, 202, 399
HpyCH4III ACNGT 2 cut(s) 100, 181
HpyCH4IV ACGT 2 cut(s) 267, 314
HpyCH4V TGCA 2 cut(s) 193, 309
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 132, 494
HpyF3I CTNAG 5 cut(s) 21, 170, 221, 281, 329
HpySE526I ACGT 2 cut(s) 267, 314
Hsp92I GRCGYC 2 cut(s) 267, 369
HspAI GCGC 1 cut(s) 369
KasI GGCGCC 1 cut(s) 368
Kzo9I GATC 6 cut(s) 112, 117, 144, 286, 461, 499
LmnI GCTCC 4 cut(s) 86, 218, 409, 445
LpnPI CCDG 9 cut(s) 35, 86, 182, 280, 359, 389, 420, 462, 488
Lsp1109I GCAGC 2 cut(s) 205, 428
LweI GCATC 1 cut(s) 318
MaeI CTAG 1 cut(s) 523
MaeII ACGT 2 cut(s) 267, 314
MaeIII GTNAC 1 cut(s) 247
MalI GATC 6 cut(s) 114, 119, 146, 288, 463, 501
MbiI CCGCTC 2 cut(s) 213, 404
MboI GATC 6 cut(s) 112, 117, 144, 286, 461, 499
MboII GAAGA 3 cut(s) 154, 262, 484
MflI RGATCY 2 cut(s) 144, 499
MluCI AATT 1 cut(s) 508
Mly113I GGCGCC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 406
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 2 cut(s) 346, 449
Mva1269I GAATGC 2 cut(s) 40, 126
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 3 cut(s) 17, 132, 494
NarI GGCGCC 1 cut(s) 369
NdeII GATC 6 cut(s) 112, 117, 144, 286, 461, 499
NlaIV GGNNCC 2 cut(s) 350, 370
NmeAIII GCCGAG 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PctI GAATGC 2 cut(s) 40, 126
PinAI ACCGGT 2 cut(s) 345, 448
PkrI GCNGC 3 cut(s) 195, 212, 418
PleI GAGTC 1 cut(s) 404
PluTI GGCGCC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 350, 370
PspPI GGNCC 3 cut(s) 343, 348, 483
PstI CTGCAG 1 cut(s) 195
PsuI RGATCY 2 cut(s) 144, 499
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 230
RsaNI GTAC 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 428
Rsr2I CGGWCCG 1 cut(s) 483
RsrII CGGWCCG 1 cut(s) 483
SatI GCNGC 3 cut(s) 194, 211, 417
Sau3AI GATC 6 cut(s) 112, 117, 144, 286, 461, 499
Sau96I GGNCC 3 cut(s) 343, 348, 483
ScaI AGTACT 1 cut(s) 230
SchI GAGTC 1 cut(s) 404
SetI ASST 9 cut(s) 22, 63, 90, 96, 168, 198, 270, 282, 317
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 1 cut(s) 191
SfoI GGCGCC 1 cut(s) 370
SinI GGWCC 3 cut(s) 343, 348, 483
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 1 cut(s) 508
SsiI CCGC 6 cut(s) 211, 402, 444, 469, 481, 486
SspDI GGCGCC 1 cut(s) 368
SspMI CTAG 1 cut(s) 523
TaaI ACNGT 2 cut(s) 100, 181
TaiI ACGT 2 cut(s) 270, 317
TaqI TCGA 1 cut(s) 111
TasI AATT 1 cut(s) 508
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 213
TscAI CASTG 1 cut(s) 195
TseFI GTSAC 1 cut(s) 247
TseI GCWGC 2 cut(s) 193, 416
Tsp45I GTSAC 1 cut(s) 247
TspRI CASTG 1 cut(s) 195
VpaK11BI GGWCC 3 cut(s) 343, 348, 483
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 1 cut(s) 523
ZraI GACGTC 1 cut(s) 268
ZrmI AGTACT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.