Rh2AG338000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002484
Physical Location & Seq
Forward (+)
49000313 .. 49001019
707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG338000.1

Sequence Viewer

Length: 522 bp
ATGCTGATGGAGATGGCGAGCAAAGCTAAGAATAACGCGAATGCGAAGATGTCTCTGGATGATTACGTCCTTCTCCTCCAGTCTGGCTCCCACCTCCACCTCACCGTCAGCCATCTCAATCAGATCATCAGCATGCACGGCTACAAGAAGATCCACAAAGTTCACAAGAAGCTTCTGAGCGACGCCGTGAACTCACTGCAGCTGGTGAACCCGTGCCGCTCCACGCTTAGGGACTACATCTCGCCGTTTGTGACGACGAAGATGGATGACGTCATCGCCGACCTCAGCAATCTCAACTGGCAAGACTGTTGCATCACGTCCATCCAAACCCTTAGTTCGTGGGAGGACCAGTCCCCTCCTCCCCGAAACGGCGCCGTCCGGTTCCAACACTACTCGCCGTCCACCGCTCCGGACTCCGCAGCCCACAGCGTGATCGTTTCTGCTCCCGGACCGACCAAGAAGAGATCGGCGCGGAAGAGGAAGCGGACTGTTAAAGTGGCCGATCTGGTAAATTTCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.24

Weight (kDa)

9.65

Isoelectric Point (pI)

56.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 14 - 70 3.7e-24 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AccB1I GGYRCC 1 cut(s) 371
AccBSI CCGCTC 2 cut(s) 219, 407
AccII CGCG 2 cut(s) 38, 472
AccIII TCCGGA 1 cut(s) 409
AciI CCGC 5 cut(s) 217, 405, 417, 472, 484
AclWI GGATC 1 cut(s) 145
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 1 cut(s) 511
AcyI GRCGYC 3 cut(s) 183, 270, 372
AdeI CACNNNGTG 1 cut(s) 430
AfiI CCNNNNNNNGG 2 cut(s) 228, 368
AjiI CACGTC 1 cut(s) 318
AluBI AGCT 3 cut(s) 26, 172, 202
AluI AGCT 3 cut(s) 26, 172, 202
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 409
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 2 cut(s) 199, 419
ApoI RAATTY 1 cut(s) 511
AspLEI GCGC 2 cut(s) 374, 472
AspS9I GGNCC 2 cut(s) 346, 449
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 2 cut(s) 94, 217
AvaII GGWCC 2 cut(s) 346, 449
BanI GGYRCC 1 cut(s) 371
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 211, 431
BccI CCATC 4 cut(s) 7, 120, 256, 329
BceAI ACGGC 6 cut(s) 154, 170, 229, 359, 382, 385
BcnI CCSGG 1 cut(s) 447
BcoDI GTCTC 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 197
BfoI RGCGCY 1 cut(s) 375
BisI GCNGC 3 cut(s) 200, 217, 420
BlsI GCNGC 3 cut(s) 201, 218, 421
Bme1390I CCNGG 1 cut(s) 447
Bme18I GGWCC 2 cut(s) 346, 449
BmgBI CACGTC 1 cut(s) 318
BmgT120I GGNCC 2 cut(s) 346, 449
BmiI GGNNCC 3 cut(s) 88, 373, 383
BmrFI CCNGG 1 cut(s) 447
BmsI GCATC 1 cut(s) 321
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 2 cut(s) 227, 284
BpuMI CCSGG 1 cut(s) 447
BsaHI GRCGYC 3 cut(s) 183, 270, 372
BsaWI WCCGGW 2 cut(s) 378, 409
BsaXI ACNNNNNCTCC 2 cut(s) 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 368
Bse1I ACTGG 3 cut(s) 79, 302, 349
BseAI TCCGGA 1 cut(s) 409
BseGI GGATG 3 cut(s) 64, 271, 321
BseLI CCNNNNNNNGG 2 cut(s) 228, 368
BseMII CTCAG 2 cut(s) 167, 298
BseNI ACTGG 3 cut(s) 79, 302, 349
BseRI GAGGAG 2 cut(s) 65, 348
BseXI GCAGC 2 cut(s) 211, 431
Bsh1236I CGCG 2 cut(s) 38, 472
BshFI GGCC 1 cut(s) 500
BshNI GGYRCC 1 cut(s) 371
BsiSI CCGG 3 cut(s) 379, 410, 447
BslFI GGGAC 2 cut(s) 245, 337
BslI CCNNNNNNNGG 2 cut(s) 228, 368
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 245, 337
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 1 cut(s) 500
Bsp13I TCCGGA 1 cut(s) 409
Bsp143I GATC 5 cut(s) 123, 150, 432, 464, 502
BspACI CCGC 5 cut(s) 217, 405, 417, 472, 484
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 2 cut(s) 168, 297
BspEI TCCGGA 1 cut(s) 409
BspFNI CGCG 2 cut(s) 38, 472
BspLI GGNNCC 3 cut(s) 88, 373, 383
BspMAI CTGCAG 1 cut(s) 201
BspPI GGATC 1 cut(s) 145
BspT107I GGYRCC 1 cut(s) 371
BsrBI CCGCTC 2 cut(s) 219, 407
BsrI ACTGG 3 cut(s) 79, 302, 349
BssMI GATC 5 cut(s) 123, 150, 432, 464, 502
BssNI GRCGYC 3 cut(s) 183, 270, 372
Bst4CI ACNGT 3 cut(s) 106, 308, 490
Bst6I CTCTTC 2 cut(s) 455, 470
BstACI GRCGYC 3 cut(s) 183, 270, 372
BstC8I GCNNGC 2 cut(s) 19, 134
BstDEI CTNAG 5 cut(s) 27, 176, 227, 284, 332
BstF5I GGATG 3 cut(s) 64, 271, 321
BstFNI CGCG 2 cut(s) 38, 472
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 2 cut(s) 374, 472
BstKTI GATC 5 cut(s) 126, 153, 435, 467, 505
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 5 cut(s) 123, 150, 432, 464, 502
BstMWI GCNNNNNNNGC 2 cut(s) 23, 138
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 1 cut(s) 445
BstSFI CTRYAG 1 cut(s) 197
BstUI CGCG 2 cut(s) 38, 472
BstV1I GCAGC 2 cut(s) 211, 431
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 1 cut(s) 500
BtgZI GCGATG 1 cut(s) 259
BtrI CACGTC 1 cut(s) 318
BtsCI GGATG 3 cut(s) 64, 271, 321
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 19, 134
CfoI GCGC 2 cut(s) 374, 472
Cfr13I GGNCC 2 cut(s) 346, 449
CpoI CGGWCCG 1 cut(s) 449
CseI GACGC 1 cut(s) 191
CspI CGGWCCG 1 cut(s) 449
CviAII CATG 1 cut(s) 133
CviJI RGCY 8 cut(s) 26, 87, 111, 141, 172, 202, 422, 500
CviKI_1 RGCY 8 cut(s) 26, 87, 111, 141, 172, 202, 422, 500
DdeI CTNAG 5 cut(s) 27, 176, 227, 284, 332
DinI GGCGCC 1 cut(s) 373
DpnI GATC 5 cut(s) 125, 152, 434, 466, 504
DpnII GATC 5 cut(s) 123, 150, 432, 464, 502
DraIII CACNNNGTG 1 cut(s) 430
EaeI YGGCCR 1 cut(s) 498
Eam1104I CTCTTC 2 cut(s) 455, 470
EarI CTCTTC 2 cut(s) 455, 470
Eco47I GGWCC 2 cut(s) 346, 449
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
FaeI CATG 1 cut(s) 136
FaiI YATR 1 cut(s) 134
FaqI GGGAC 2 cut(s) 245, 337
FatI CATG 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 200, 217, 420
FokI GGATG 3 cut(s) 71, 278, 308
Fsp4HI GCNGC 3 cut(s) 200, 217, 420
GlaI GCGC 2 cut(s) 373, 471
GluI GCNGC 3 cut(s) 200, 217, 420
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 1 cut(s) 500
HapII CCGG 3 cut(s) 379, 410, 447
HgaI GACGC 1 cut(s) 191
HhaI GCGC 2 cut(s) 374, 472
Hin1I GRCGYC 3 cut(s) 183, 270, 372
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 2 cut(s) 372, 470
HinP1I GCGC 2 cut(s) 372, 470
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 1 cut(s) 413
HpaII CCGG 3 cut(s) 379, 410, 447
HphI GGTGA 2 cut(s) 94, 217
Hpy166II GTNNAC 4 cut(s) 163, 190, 208, 402
Hpy188I TCNGA 2 cut(s) 123, 177
Hpy188III TCNNGA 2 cut(s) 56, 410
Hpy8I GTNNAC 4 cut(s) 163, 190, 208, 402
Hpy99I CGWCG 2 cut(s) 185, 259
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 3 cut(s) 106, 308, 490
HpyCH4IV ACGT 3 cut(s) 66, 270, 317
HpyCH4V TGCA 3 cut(s) 136, 199, 312
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 138
HpyF3I CTNAG 5 cut(s) 27, 176, 227, 284, 332
HpySE526I ACGT 3 cut(s) 66, 270, 317
Hsp92I GRCGYC 3 cut(s) 183, 270, 372
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 2 cut(s) 372, 470
KasI GGCGCC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 409
Kzo9I GATC 5 cut(s) 123, 150, 432, 464, 502
LmnI GCTCC 4 cut(s) 92, 224, 412, 448
Lsp1109I GCAGC 2 cut(s) 211, 431
LweI GCATC 1 cut(s) 321
MaeII ACGT 3 cut(s) 66, 270, 317
MaeIII GTNAC 1 cut(s) 250
MalI GATC 5 cut(s) 125, 152, 434, 466, 504
MbiI CCGCTC 2 cut(s) 219, 407
MboI GATC 5 cut(s) 123, 150, 432, 464, 502
MboII GAAGA 5 cut(s) 58, 160, 271, 472, 487
MflI RGATCY 1 cut(s) 150
MluCI AATT 1 cut(s) 511
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 8 cut(s) 86, 104, 110, 293, 337, 366, 369, 471
MroI TCCGGA 1 cut(s) 409
MseI TTAA 1 cut(s) 492
MslI CAYNNNNRTG 1 cut(s) 131
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 3 cut(s) 379, 410, 447
MspR9I CCNGG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 46
MvnI CGCG 2 cut(s) 38, 472
MwoI GCNNNNNNNGC 2 cut(s) 23, 138
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 1 cut(s) 447
NdeII GATC 5 cut(s) 123, 150, 432, 464, 502
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 3 cut(s) 88, 373, 383
NmuCI GTSAC 1 cut(s) 250
NspI RCATGY 1 cut(s) 136
PaeI GCATGC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 3 cut(s) 201, 218, 421
PleI GAGTC 1 cut(s) 407
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 407
PspN4I GGNNCC 3 cut(s) 88, 373, 383
PspPI GGNCC 2 cut(s) 346, 449
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 131
Rsr2I CGGWCCG 1 cut(s) 449
RsrII CGGWCCG 1 cut(s) 449
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 3 cut(s) 200, 217, 420
Sau3AI GATC 5 cut(s) 123, 150, 432, 464, 502
Sau96I GGNCC 2 cut(s) 346, 449
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 1 cut(s) 447
SetI ASST 9 cut(s) 28, 69, 96, 102, 174, 204, 273, 285, 320
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 197
SfoI GGCGCC 1 cut(s) 373
SinI GGWCC 2 cut(s) 346, 449
SmiMI CAYNNNNRTG 1 cut(s) 131
SphI GCATGC 1 cut(s) 136
Sse9I AATT 1 cut(s) 511
SsiI CCGC 5 cut(s) 217, 405, 417, 472, 484
SspDI GGCGCC 1 cut(s) 371
StyD4I CCNGG 1 cut(s) 445
TaaI ACNGT 3 cut(s) 106, 308, 490
TaiI ACGT 3 cut(s) 69, 273, 320
TaqII GACCGA 1 cut(s) 466
TasI AATT 1 cut(s) 511
TauI GCSGC 1 cut(s) 219
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 201
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 199, 419
Tsp45I GTSAC 1 cut(s) 250
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 2 cut(s) 346, 449
XapI RAATTY 1 cut(s) 511
XceI RCATGY 1 cut(s) 136
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.