MD09G1238400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002484
Physical Location & Seq
Reverse (-)
30332374 .. 30333885
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1238400.v1.1.491

Sequence Viewer

Length: 633 bp
ATGTCGAGTGCGAGTGCGAGTGGGAGAAAAAGGAAGACGACGAGTTTCCAGAGAGAAAAGATGTTTCTAGAAGATTACCTTCTCCTCATTCAGTCCAACTCCCACCTCCATCTCACCGTCGCTCATCTCAATCAGATCATTGGCATGCACGGATACAAGAAGATTTACAAAGTTCCCAAGAAACGTTTTAGCGACGCCGTGAGTTCGCTGTCGCTTGTCGATCCGGCTCGCTCGACGCTCAAAGACTACATCTCGCCGTTTGTGATCACCACCCTGGAGGACGTCGTCGCTGACCTCGCCGACCTCAACTGGAAGGAGTGCTGCGTCACCTCCGTTGAAACCCTAAGCTCGTGGAAACACACAACCTCCGCTCCTGCTGCTCCTGCGAGCCCCCGCCTCGACCTCGTCCAGTACTCCCAGCTTCAACAATCAACGCCGTTGGCCCTTGATTCCAGACTCTACGGCGTCGTTTCGGCTCCTAACTCTGCTTCCGAGACCGCTCCTCCAGCGAAGAAACTGGTGACGAAGAGGAGGAGGAAGCGATCGCCGATAGCCGCCGGTTTCGCTCGTGCTCTGGACTCATTTTCACTTACGTGTTGGCGGGAGTTGGGCACTTGTTTGGTAGAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.24

Weight (kDa)

9.58

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7787 PF25042 18 - 74 1.7e-23 Domain of unknown function (DUF7787)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 285
AccBSI CCGCTC 2 cut(s) 371, 500
AciI CCGC 5 cut(s) 369, 394, 498, 555, 601
AclI AACGTT 1 cut(s) 184
AclWI GGATC 1 cut(s) 215
AcyI GRCGYC 3 cut(s) 195, 282, 465
AfaI GTAC 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 2 cut(s) 338, 425
AjnI CCWGG 1 cut(s) 273
AleI CACNNNNGTG 1 cut(s) 592
AluBI AGCT 3 cut(s) 348, 421, 628
AluI AGCT 3 cut(s) 348, 421, 628
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 1 cut(s) 215
AoxI GGCC 1 cut(s) 441
ApeKI GCWGC 2 cut(s) 321, 377
AsiSI GCGATCGC 1 cut(s) 545
AspS9I GGNCC 1 cut(s) 442
AsuHPI GGTGA 4 cut(s) 106, 259, 319, 532
BaeGI GKGCMC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 392
BauI CACGAG 2 cut(s) 349, 567
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 2 cut(s) 308, 364
BccI CCATC 1 cut(s) 117
BceAI ACGGC 4 cut(s) 182, 241, 421, 478
BciT130I CCWGG 1 cut(s) 275
BciVI GTATCC 1 cut(s) 146
BclI TGATCA 1 cut(s) 264
BcoDI GTCTC 1 cut(s) 488
BfaI CTAG 1 cut(s) 68
BfuI GTATCC 1 cut(s) 146
BisI GCNGC 3 cut(s) 322, 378, 555
BlsI GCNGC 3 cut(s) 323, 379, 556
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 275
BpiI GAAGAC 1 cut(s) 41
BpmI CTGGAG 2 cut(s) 296, 489
Bpu10I CCTNAGC 1 cut(s) 344
BsaAI YACGTR 1 cut(s) 594
BsaHI GRCGYC 3 cut(s) 195, 282, 465
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 1 cut(s) 273
BsaXI ACNNNNNCTCC 6 cut(s) 350, 355, 380, 385, 487, 517
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 314, 409, 522
BseBI CCWGG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 273
BseNI ACTGG 3 cut(s) 314, 409, 522
BseRI GAGGAG 4 cut(s) 74, 492, 544, 547
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 2 cut(s) 308, 364
BseYI CCCAGC 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 545
BshFI GGCC 1 cut(s) 443
BsiEI CGRYCG 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 574
BsiSI CCGG 2 cut(s) 224, 558
BsmAI GTCTC 1 cut(s) 488
BsnI GGCC 1 cut(s) 443
Bso31I GGTCTC 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 392, 574, 614
Bsp143I GATC 4 cut(s) 135, 220, 264, 542
BspACI CCGC 5 cut(s) 369, 394, 498, 555, 601
BspANI GGCC 1 cut(s) 443
BspLI GGNNCC 1 cut(s) 477
BspPI GGATC 1 cut(s) 215
BspTNI GGTCTC 1 cut(s) 488
BsrBI CCGCTC 2 cut(s) 371, 500
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 3 cut(s) 314, 409, 522
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 4 cut(s) 135, 220, 264, 542
BssNI GRCGYC 3 cut(s) 195, 282, 465
BssSI CACGAG 2 cut(s) 349, 567
Bst2BI CACGAG 2 cut(s) 349, 567
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 521
BstACI GRCGYC 3 cut(s) 195, 282, 465
BstBAI YACGTR 1 cut(s) 594
BstC8I GCNNGC 3 cut(s) 146, 229, 388
BstDEI CTNAG 1 cut(s) 344
BstKTI GATC 4 cut(s) 138, 223, 267, 545
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 4 cut(s) 135, 220, 264, 542
BstMCI CGRYCG 1 cut(s) 545
BstMWI GCNNNNNNNGC 5 cut(s) 296, 377, 383, 506, 563
BstNI CCWGG 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 148
BstSCI CCNGG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 614
BstV1I GCAGC 2 cut(s) 308, 364
BstV2I GAAGAC 1 cut(s) 41
BsuI GTATCC 1 cut(s) 146
BsuRI GGCC 1 cut(s) 443
Cac8I GCNNGC 3 cut(s) 146, 229, 388
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 1 cut(s) 442
CseI GACGC 4 cut(s) 203, 244, 313, 454
Csp6I GTAC 1 cut(s) 412
CviAII CATG 1 cut(s) 145
CviJI RGCY 8 cut(s) 227, 348, 390, 421, 443, 476, 554, 628
CviKI_1 RGCY 8 cut(s) 227, 348, 390, 421, 443, 476, 554, 628
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 1 cut(s) 344
DpnI GATC 4 cut(s) 137, 222, 266, 544
DpnII GATC 4 cut(s) 135, 220, 264, 542
Eam1104I CTCTTC 1 cut(s) 521
EarI CTCTTC 1 cut(s) 521
Eco24I GRGCYC 1 cut(s) 392
Eco31I GGTCTC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 392
FaeI CATG 1 cut(s) 148
FaiI YATR 2 cut(s) 146, 631
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FatI CATG 1 cut(s) 144
FauI CCCGC 2 cut(s) 401, 594
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 3 cut(s) 322, 378, 555
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 3 cut(s) 322, 378, 555
FspBI CTAG 1 cut(s) 68
GluI GCNGC 3 cut(s) 322, 378, 555
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 2 cut(s) 296, 489
HaeIII GGCC 1 cut(s) 443
HapII CCGG 2 cut(s) 224, 558
HgaI GACGC 4 cut(s) 203, 244, 313, 454
Hin1I GRCGYC 3 cut(s) 195, 282, 465
Hin1II CATG 1 cut(s) 148
HinfI GANTC 3 cut(s) 449, 456, 578
HpaII CCGG 2 cut(s) 224, 558
HphI GGTGA 4 cut(s) 106, 259, 319, 532
Hpy188I TCNGA 2 cut(s) 135, 493
Hpy188III TCNNGA 4 cut(s) 49, 68, 453, 575
Hpy99I CGWCG 7 cut(s) 43, 122, 197, 238, 287, 290, 470
HpyAV CCTTC 2 cut(s) 89, 307
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 184, 282, 593
HpyCH4V TGCA 1 cut(s) 148
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 377, 383, 506, 563
HpyF3I CTNAG 1 cut(s) 344
HpySE526I ACGT 3 cut(s) 184, 282, 593
Hsp92I GRCGYC 3 cut(s) 195, 282, 465
Hsp92II CATG 1 cut(s) 148
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 4 cut(s) 135, 220, 264, 542
LmnI GCTCC 4 cut(s) 376, 385, 481, 505
Lsp1109I GCAGC 2 cut(s) 308, 364
MaeI CTAG 1 cut(s) 68
MaeII ACGT 3 cut(s) 184, 282, 593
MaeIII GTNAC 2 cut(s) 325, 520
MalI GATC 4 cut(s) 137, 222, 266, 544
MbiI CCGCTC 2 cut(s) 371, 500
MboI GATC 4 cut(s) 135, 220, 264, 542
MboII GAAGA 5 cut(s) 46, 83, 172, 523, 538
MhlI GDGCHC 3 cut(s) 392, 574, 614
MlyI GAGTC 2 cut(s) 450, 572
MmeI TCCRAC 1 cut(s) 120
MslI CAYNNNNRTG 2 cut(s) 143, 592
MspI CCGG 2 cut(s) 224, 558
MspR9I CCNGG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 275
MwoI GCNNNNNNNGC 5 cut(s) 296, 377, 383, 506, 563
NdeII GATC 4 cut(s) 135, 220, 264, 542
NlaIII CATG 1 cut(s) 148
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 2 cut(s) 325, 520
NspI RCATGY 1 cut(s) 148
OliI CACNNNNGTG 1 cut(s) 592
PaeI GCATGC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 449
PflFI GACNNNGTC 2 cut(s) 284, 404
PkrI GCNGC 3 cut(s) 323, 379, 556
Ple19I CGATCG 1 cut(s) 545
PleI GAGTC 2 cut(s) 450, 572
PpsI GAGTC 2 cut(s) 450, 572
Ppu21I YACGTR 1 cut(s) 594
Psp1406I AACGTT 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 417
PspGI CCWGG 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 477
PspPI GGNCC 1 cut(s) 442
PsyI GACNNNGTC 2 cut(s) 284, 404
PvuI CGATCG 1 cut(s) 545
RgaI GCGATCGC 1 cut(s) 545
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 2 cut(s) 143, 592
SatI GCNGC 3 cut(s) 322, 378, 555
Sau3AI GATC 4 cut(s) 135, 220, 264, 542
Sau96I GGNCC 1 cut(s) 442
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 2 cut(s) 450, 572
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 3 cut(s) 392, 574, 614
SfaAI GCGATCGC 1 cut(s) 545
SgfI GCGATCGC 1 cut(s) 545
SmiMI CAYNNNNRTG 2 cut(s) 143, 592
SphI GCATGC 1 cut(s) 148
SsiI CCGC 5 cut(s) 369, 394, 498, 555, 601
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 3 cut(s) 187, 285, 596
TaqI TCGA 4 cut(s) 5, 219, 233, 399
TatI WGTACW 1 cut(s) 411
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 1 cut(s) 449
TseFI GTSAC 2 cut(s) 325, 520
TseI GCWGC 2 cut(s) 321, 377
Tsp45I GTSAC 2 cut(s) 325, 520
TspGWI ACGGA 2 cut(s) 165, 322
Tth111I GACNNNGTC 2 cut(s) 284, 404
XbaI TCTAGA 1 cut(s) 67
XceI RCATGY 1 cut(s) 148
XspI CTAG 1 cut(s) 68
ZraI GACGTC 1 cut(s) 283
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.