FvH4_6g40690

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004106
Physical Location & Seq
Forward (+)
32148137 .. 32150843
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40690.t1

Sequence Viewer

Length: 462 bp
ATGCCAACATGGACGGAGGTCGGCTCCTCGTTGGGCCGGTGCAGCATGTACAACAACATGCACCATCCAAGCAAGCTGACTCCTGGTTACGACTTTTACCTCTTCAAAGAAAATATTGAGCCAAAGTGGGAGGATCCTGTTTGTGCTACTGGAGGGAAATGGACTATGACATTTTCTAATGGGGAATCGGATCGTTCTTGGATGGATATGTTGCAAGCACTAGTCAAGGAAGAGTTTAAACATCGAGATGAAATTTGTGGAGCAGTTGTGAATGTCAGGAATGGGCAAGAAAGCATTGCTCTTTGGACCAAAAATGCTACAAATGAGGCTGCTCAGGTGAGTATTGGCAAACAATGGAAGGGCTTTCTGGATAGCAAGGAAACTATAGTCTTCACAATTCATGCTGGGCAGGATGATGCTAGCCAAGTGGGGAGCACTGCCACGAATTTGTACACAGTTTGA

Protein Analysis

154

Amino Acids

17.18

Weight (kDa)

5.32

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 14 - 127 1.3e-37 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 128, 141, 198
AcsI RAATTY 2 cut(s) 252, 445
AfaI GTAC 2 cut(s) 50, 452
AgsI TTSAA 1 cut(s) 106
AhlI ACTAGT 1 cut(s) 220
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw21I GWGCWC 1 cut(s) 437
AlwI GGATC 3 cut(s) 128, 141, 198
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 42, 329
ApoI RAATTY 2 cut(s) 252, 445
AspS9I GGNCC 2 cut(s) 34, 306
AsuHPI GGTGA 1 cut(s) 349
AsuNHI GCTAGC 1 cut(s) 419
AvaII GGWCC 1 cut(s) 306
BamHI GGATCC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 2 cut(s) 54, 316
BccI CCATC 2 cut(s) 72, 196
BciT130I CCWGG 1 cut(s) 84
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 2 cut(s) 221, 420
BfmI CTRYAG 1 cut(s) 384
BisI GCNGC 2 cut(s) 43, 330
BlsI GCNGC 2 cut(s) 44, 331
Bme1390I CCNGG 1 cut(s) 84
Bme18I GGWCC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 34, 306
BmiI GGNNCC 2 cut(s) 25, 135
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 1 cut(s) 406
BmtI GCTAGC 1 cut(s) 423
BoxI GACNNNNGTC 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 382
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 333
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 84
BseGI GGATG 3 cut(s) 64, 207, 418
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 1 cut(s) 16
BseXI GCAGC 2 cut(s) 54, 316
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 61
BshFI GGCC 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 37
BsnI GGCC 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 437
Bsp1407I TGTACA 2 cut(s) 48, 450
Bsp143I GATC 2 cut(s) 133, 190
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 1 cut(s) 346
BspLI GGNNCC 2 cut(s) 25, 135
BspOI GCTAGC 1 cut(s) 423
BspPI GGATC 3 cut(s) 128, 141, 198
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 36
BsrGI TGTACA 2 cut(s) 48, 450
BsrI ACTGG 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 36
BssMI GATC 2 cut(s) 133, 190
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 2 cut(s) 107, 225
BstAUI TGTACA 2 cut(s) 48, 450
BstC8I GCNNGC 3 cut(s) 74, 216, 421
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 64, 207, 418
BstKTI GATC 2 cut(s) 136, 193
BstMBI GATC 2 cut(s) 133, 190
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 2 cut(s) 49, 61
BstPAI GACNNNNGTC 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 82
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 54, 316
BstV2I GAAGAC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 36
BtsCI GGATG 3 cut(s) 64, 207, 418
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 3 cut(s) 74, 216, 421
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 2 cut(s) 34, 306
Csp6I GTAC 2 cut(s) 49, 451
CviAII CATG 4 cut(s) 9, 46, 58, 401
CviJI RGCY 7 cut(s) 24, 36, 76, 121, 329, 363, 423
CviKI_1 RGCY 7 cut(s) 24, 36, 76, 121, 329, 363, 423
CviQI GTAC 2 cut(s) 49, 451
DdeI CTNAG 1 cut(s) 333
DpnI GATC 2 cut(s) 135, 192
DpnII GATC 2 cut(s) 133, 190
DraI TTTAAA 1 cut(s) 238
Eam1104I CTCTTC 2 cut(s) 107, 225
EarI CTCTTC 2 cut(s) 107, 225
Eco47I GGWCC 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 82
FaeI CATG 4 cut(s) 12, 49, 61, 404
FaiI YATR 7 cut(s) 10, 47, 59, 167, 209, 386, 402
FatI CATG 4 cut(s) 8, 45, 57, 400
Fnu4HI GCNGC 2 cut(s) 43, 330
FokI GGATG 3 cut(s) 51, 214, 425
Fsp4HI GCNGC 2 cut(s) 43, 330
FspBI CTAG 2 cut(s) 221, 420
GluI GCNGC 2 cut(s) 43, 330
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 37
Hin1II CATG 4 cut(s) 12, 49, 61, 404
HinfI GANTC 2 cut(s) 79, 185
HpaII CCGG 1 cut(s) 37
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 3 cut(s) 245, 277, 368
Hpy8I GTNNAC 1 cut(s) 453
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4V TGCA 3 cut(s) 42, 61, 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 1 cut(s) 333
Hsp92II CATG 4 cut(s) 12, 49, 61, 404
Kzo9I GATC 2 cut(s) 133, 190
LmnI GCTCC 3 cut(s) 29, 260, 432
Lsp1109I GCAGC 2 cut(s) 54, 316
LweI GCATC 1 cut(s) 406
MaeI CTAG 2 cut(s) 221, 420
MaeIII GTNAC 1 cut(s) 86
MalI GATC 2 cut(s) 135, 192
MboI GATC 2 cut(s) 133, 190
MboII GAAGA 3 cut(s) 94, 242, 382
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 3 cut(s) 252, 396, 445
MlyI GAGTC 1 cut(s) 73
MnlI CCTC 6 cut(s) 10, 37, 110, 124, 146, 319
MseI TTAA 1 cut(s) 237
MslI CAYNNNNRTG 1 cut(s) 246
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 84
MssI GTTTAAAC 1 cut(s) 238
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 42
NdeII GATC 2 cut(s) 133, 190
NheI GCTAGC 1 cut(s) 419
NlaIII CATG 4 cut(s) 12, 49, 61, 404
NlaIV GGNNCC 2 cut(s) 25, 135
NspI RCATGY 2 cut(s) 49, 61
PfeI GAWTC 1 cut(s) 185
PkrI GCNGC 2 cut(s) 44, 331
PleI GAGTC 1 cut(s) 73
PmeI GTTTAAAC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 73
PshAI GACNNNNGTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 2 cut(s) 25, 135
PspPI GGNCC 2 cut(s) 34, 306
PsuI RGATCY 1 cut(s) 133
RsaI GTAC 2 cut(s) 50, 452
RsaNI GTAC 2 cut(s) 49, 451
RseI CAYNNNNRTG 1 cut(s) 246
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 2 cut(s) 43, 330
Sau3AI GATC 2 cut(s) 133, 190
Sau96I GGNCC 2 cut(s) 34, 306
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 1 cut(s) 437
SetI ASST 4 cut(s) 21, 78, 102, 339
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 384
SinI GGWCC 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 246
SpeI ACTAGT 1 cut(s) 220
Sse9I AATT 3 cut(s) 252, 396, 445
SspI AATATT 1 cut(s) 115
SspMI CTAG 2 cut(s) 221, 420
StyD4I CCNGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 457
TaqI TCGA 1 cut(s) 244
TasI AATT 3 cut(s) 252, 396, 445
TatI WGTACW 2 cut(s) 48, 450
TfiI GAWTC 1 cut(s) 185
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 442
TseI GCWGC 2 cut(s) 42, 329
TspDTI ATGAA 2 cut(s) 264, 389
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 306
XapI RAATTY 2 cut(s) 252, 445
XceI RCATGY 2 cut(s) 49, 61
XspI CTAG 2 cut(s) 221, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.