Rh5BG094900

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004106
Physical Location & Seq
Forward (+)
8616581 .. 8618783
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG094900.1

Sequence Viewer

Length: 699 bp
ATGGCGGTGGAAGACGCAGGCAAGACTTCAGCTTCGGAAGATCAGAGCAAGACGGAGTCGAACCCTAAATCAATGCAAGACGAGAACGAGCTGGAAGAAGGAGAGATCGTCGGCGGCGGCGGCGACGAGGTGTCTTCGAAGATCGCAAAGCCGCTGCACCACCCTCTGGAGCACTCATGGACCTTCTGGTTCGATAGCCCCTCGGCCAAGAACTCCAAGCCCAAGCAAGAGGACTGGGGCAGCTCTATTCGCCCGATCTACACCTTCTCTACTGTTGAGGAATTCTGGGGTATATACAATAATATACGCCATCCAAGCAAGTTGTCTATGGGGACGGACCTCCATTGTTTCAAAAACAAAATTGAGCCTAAGTGGGAGGATCCTGTTTGTGCTAATGGAGGGAAATGGACTCTAACTTTTACTAAAGGGAAATCTGATCATCCCTGGCTGCATACGTTGCTAGCACTGATTGGGGAACAGTTTGATCATGGAGATGAAATATGTGGAGCAGTTGTCAATGTCAGAACTAGGCAAGAAAAAATTGCTATTTGGACCAAGAATGCAGCAAATGAGCCTGCTCAGATGAGCATTGGAAAACAATGGAAGGGCTTTCTGGATAACAACGAAACTATAGGCTTCATATTTCATGACGATGCAAGGCGACAGGAGAGAGGTGCCAAGAATAGATACACAGTGTGA

Protein Analysis

232

Amino Acids

26.22

Weight (kDa)

5.74

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 55 - 208 2.3e-57 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 1 cut(s) 166
AciI CCGC 5 cut(s) 5, 114, 117, 120, 152
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 1 cut(s) 12
AdeI CACNNNGTG 1 cut(s) 696
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 1 cut(s) 352
AhdI GACNNNNNGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 3 cut(s) 32, 91, 243
AluI AGCT 3 cut(s) 32, 91, 243
Alw21I GWGCWC 1 cut(s) 174
AlwI GGATC 2 cut(s) 374, 387
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 4 cut(s) 154, 240, 448, 563
ApoI RAATTY 1 cut(s) 281
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AspS9I GGNCC 3 cut(s) 180, 337, 552
AsuII TTCGAA 1 cut(s) 137
AsuNHI GCTAGC 1 cut(s) 460
AvaII GGWCC 3 cut(s) 180, 337, 552
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 674
BbsI GAAGAC 2 cut(s) 18, 126
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 4 cut(s) 141, 252, 435, 575
BccI CCATC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 445
BclI TGATCA 2 cut(s) 436, 484
BfaI CTAG 2 cut(s) 461, 528
BfmI CTRYAG 1 cut(s) 630
BisI GCNGC 8 cut(s) 115, 118, 121, 152, 155, 241, 449, 564
BlsI GCNGC 8 cut(s) 116, 119, 122, 153, 156, 242, 450, 565
Bme1390I CCNGG 1 cut(s) 445
Bme18I GGWCC 3 cut(s) 180, 337, 552
BmeRI GACNNNNNGTC 1 cut(s) 130
BmgT120I GGNCC 3 cut(s) 180, 337, 552
BmiI GGNNCC 2 cut(s) 381, 676
BmrFI CCNGG 1 cut(s) 445
BmrI ACTGGG 1 cut(s) 244
BmsI GCATC 1 cut(s) 643
BmtI GCTAGC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 244
BpiI GAAGAC 2 cut(s) 18, 126
BpmI CTGGAG 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 137
BsaJI CCNNGG 2 cut(s) 201, 443
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 498, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 1 cut(s) 239
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 201, 443
BseGI GGATG 2 cut(s) 310, 439
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 1 cut(s) 239
BseXI GCAGC 4 cut(s) 141, 252, 435, 575
BsgI GTGCAG 1 cut(s) 140
BshFI GGCC 1 cut(s) 206
BshNI GGYRCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 174
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 1 cut(s) 206
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
BspACI CCGC 5 cut(s) 5, 114, 117, 120, 152
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 592
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 381, 676
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 374, 387
BspT104I TTCGAA 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 674
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 201, 443
BssMI GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 3 cut(s) 274, 480, 694
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 3 cut(s) 19, 462, 576
BstDEI CTNAG 2 cut(s) 369, 579
BstF5I GGATG 2 cut(s) 310, 439
BstKTI GATC 7 cut(s) 43, 108, 144, 258, 382, 439, 487
BstMBI GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
BstMWI GCNNNNNNNGC 4 cut(s) 120, 249, 315, 457
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 630
BstV1I GCAGC 4 cut(s) 141, 252, 435, 575
BstV2I GAAGAC 2 cut(s) 18, 126
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 2 cut(s) 310, 439
BtsIMutI CAGTG 2 cut(s) 464, 699
Cac8I GCNNGC 3 cut(s) 19, 462, 576
CciI TCATGA 1 cut(s) 646
Cfr13I GGNCC 3 cut(s) 180, 337, 552
CseI GACGC 1 cut(s) 23
CviAII CATG 3 cut(s) 177, 488, 647
DdeI CTNAG 2 cut(s) 369, 579
DpnI GATC 7 cut(s) 42, 107, 143, 257, 381, 438, 486
DpnII GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
DraIII CACNNNGTG 1 cut(s) 696
DriI GACNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 1 cut(s) 204
Eam1105I GACNNNNNGTC 1 cut(s) 130
Eco47I GGWCC 3 cut(s) 180, 337, 552
Eco57I CTGAAG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 443
FaeI CATG 3 cut(s) 180, 491, 650
FaqI GGGAC 1 cut(s) 346
FatI CATG 3 cut(s) 176, 487, 646
FbaI TGATCA 2 cut(s) 436, 484
Fnu4HI GCNGC 8 cut(s) 115, 118, 121, 152, 155, 241, 449, 564
FokI GGATG 2 cut(s) 297, 426
Fsp4HI GCNGC 8 cut(s) 115, 118, 121, 152, 155, 241, 449, 564
FspBI CTAG 2 cut(s) 461, 528
GluI GCNGC 8 cut(s) 115, 118, 121, 152, 155, 241, 449, 564
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 1 cut(s) 206
HgaI GACGC 1 cut(s) 23
Hin1II CATG 3 cut(s) 180, 491, 650
HinfI GANTC 2 cut(s) 56, 409
Hpy188I TCNGA 5 cut(s) 37, 45, 436, 524, 582
Hpy188III TCNNGA 3 cut(s) 167, 614, 647
Hpy99I CGWCG 2 cut(s) 113, 128
HpyAV CCTTC 4 cut(s) 92, 193, 274, 598
HpyCH4III ACNGT 3 cut(s) 274, 480, 694
HpyCH4IV ACGT 1 cut(s) 455
HpyCH4V TGCA 5 cut(s) 76, 157, 451, 563, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 249, 315, 457
HpyF3I CTNAG 2 cut(s) 369, 579
HpySE526I ACGT 1 cut(s) 455
Hsp92II CATG 3 cut(s) 180, 491, 650
Ksp22I TGATCA 2 cut(s) 436, 484
Kzo9I GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
LmnI GCTCC 2 cut(s) 169, 506
Lsp1109I GCAGC 4 cut(s) 141, 252, 435, 575
LweI GCATC 1 cut(s) 643
MaeI CTAG 2 cut(s) 461, 528
MaeII ACGT 1 cut(s) 455
MalI GATC 7 cut(s) 42, 107, 143, 257, 381, 438, 486
MboI GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
MboII GAAGA 5 cut(s) 23, 50, 107, 126, 151
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 3 cut(s) 281, 360, 540
MlyI GAGTC 2 cut(s) 65, 403
MnlI CCTC 9 cut(s) 121, 174, 211, 223, 271, 350, 370, 392, 665
MslI CAYNNNNRTG 2 cut(s) 492, 651
MspA1I CMGCKG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 4 cut(s) 120, 249, 315, 457
NdeII GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 3 cut(s) 180, 491, 650
NlaIV GGNNCC 2 cut(s) 381, 676
NmeAIII GCCGAG 1 cut(s) 182
NspV TTCGAA 1 cut(s) 137
PagI TCATGA 1 cut(s) 646
PctI GAATGC 1 cut(s) 565
PflFI GACNNNGTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 8 cut(s) 116, 119, 122, 153, 156, 242, 450, 565
PleI GAGTC 2 cut(s) 64, 403
PpsI GAGTC 2 cut(s) 64, 403
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 381, 676
PspPI GGNCC 3 cut(s) 180, 337, 552
PsuI RGATCY 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 55
RseI CAYNNNNRTG 2 cut(s) 492, 651
SatI GCNGC 8 cut(s) 115, 118, 121, 152, 155, 241, 449, 564
Sau3AI GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
Sau96I GGNCC 3 cut(s) 180, 337, 552
SchI GAGTC 2 cut(s) 65, 403
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 1 cut(s) 174
SetI ASST 9 cut(s) 34, 93, 132, 185, 245, 266, 342, 458, 676
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 630
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 3 cut(s) 180, 337, 552
SmiMI CAYNNNNRTG 2 cut(s) 492, 651
Sse9I AATT 3 cut(s) 281, 360, 540
SsiI CCGC 5 cut(s) 5, 114, 117, 120, 152
SspMI CTAG 2 cut(s) 461, 528
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 274, 480, 694
TaiI ACGT 1 cut(s) 458
TaqI TCGA 3 cut(s) 59, 137, 192
TasI AATT 3 cut(s) 281, 360, 540
TauI GCSGC 4 cut(s) 117, 120, 123, 154
TscAI CASTG 2 cut(s) 471, 699
TseI GCWGC 4 cut(s) 154, 240, 448, 563
TspDTI ATGAA 3 cut(s) 510, 628, 635
TspGWI ACGGA 2 cut(s) 68, 350
TspRI CASTG 2 cut(s) 471, 699
Tth111I GACNNNGTC 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 166
VpaK11BI GGWCC 3 cut(s) 180, 337, 552
XapI RAATTY 1 cut(s) 281
XspI CTAG 2 cut(s) 461, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.