Rroxscaffold_4G00303860

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004106
Physical Location & Seq
Forward (+)
24353705 .. 24361748
8044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303860.1

Sequence Viewer

Length: 345 bp
ATGATCTATATTCCACAGATTGGAGGATCTGTTTGTGCTAATGGAGGGAAACGGACTTTAACTTTTTCTAGAGGCAAATGCGACTATTCCTGGTTGTGTACGTTGCAAGCACTGATCGGAGAACAATTTGATCATGGAGATGAAATATGTGGAGCAGTTGTCAATGTCAGAAATAGGCAAGAAAAAATTGCTCTTTGGACCAAGAATGCAGCAAATGAGGCTGCTCAGGTGAGCATTGGAAAACAGTGGAAGGGCATTCTGGAGAGCAACGAAACTATAGGGTTCATATTTCATGAAGATGCAGGGCAAGATGAGAGCAGTGCTAAGAATAGCTACAGAGTGTAA

Protein Analysis

114

Amino Acids

12.55

Weight (kDa)

6.08

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 12 - 91 4.7e-22 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 20
AclWI GGATC 1 cut(s) 34
AfaI GTAC 1 cut(s) 100
AfiI CCNNNNNNNGG 1 cut(s) 20
AjnI CCWGG 1 cut(s) 89
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
AlwI GGATC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 209, 221
AspS9I GGNCC 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 208, 221
BciT130I CCWGG 1 cut(s) 91
BclI TGATCA 1 cut(s) 130
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 2 cut(s) 276, 334
BisI GCNGC 2 cut(s) 210, 222
BlsI GCNGC 2 cut(s) 211, 223
Bme1390I CCNGG 1 cut(s) 91
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 289
BpmI CTGGAG 1 cut(s) 281
Bpu10I CCTNAGC 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 20
BseBI CCWGG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMII CTCAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 208, 221
BslI CCNNNNNNNGG 1 cut(s) 20
BsmI GAATGC 2 cut(s) 211, 255
Bsp143I GATC 4 cut(s) 3, 26, 114, 130
BspCNI CTCAG 1 cut(s) 238
BspHI TCATGA 1 cut(s) 292
BspPI GGATC 1 cut(s) 34
BssMI GATC 4 cut(s) 3, 26, 114, 130
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 225, 324
BstKTI GATC 4 cut(s) 6, 29, 117, 133
BstMBI GATC 4 cut(s) 3, 26, 114, 130
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 89
BstSFI CTRYAG 2 cut(s) 276, 334
BstV1I GCAGC 2 cut(s) 208, 221
BstX2I RGATCY 1 cut(s) 26
BstYI RGATCY 1 cut(s) 26
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 3 cut(s) 110, 251, 325
Cac8I GCNNGC 1 cut(s) 108
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 198
Csp6I GTAC 1 cut(s) 99
CviAII CATG 2 cut(s) 134, 293
CviJI RGCY 2 cut(s) 221, 333
CviKI_1 RGCY 2 cut(s) 221, 333
CviQI GTAC 1 cut(s) 99
DdeI CTNAG 2 cut(s) 225, 324
DpnI GATC 4 cut(s) 5, 28, 116, 132
DpnII GATC 4 cut(s) 3, 26, 114, 130
Eco47I GGWCC 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 89
FaeI CATG 2 cut(s) 137, 296
FaiI YATR 6 cut(s) 9, 135, 148, 278, 287, 294
FatI CATG 2 cut(s) 133, 292
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 210, 222
Fsp4HI GCNGC 2 cut(s) 210, 222
FspBI CTAG 1 cut(s) 69
GluI GCNGC 2 cut(s) 210, 222
GsuI CTGGAG 1 cut(s) 281
Hin1II CATG 2 cut(s) 137, 296
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 1 cut(s) 99
Hpy188I TCNGA 2 cut(s) 119, 170
Hpy188III TCNNGA 3 cut(s) 69, 260, 293
Hpy8I GTNNAC 1 cut(s) 99
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 246
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 106, 209, 302
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 225, 324
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 2 cut(s) 137, 296
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 4 cut(s) 3, 26, 114, 130
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 5 cut(s) 76, 103, 212, 245, 288
Lsp1109I GCAGC 2 cut(s) 208, 221
LweI GCATC 1 cut(s) 289
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 101
MalI GATC 4 cut(s) 5, 28, 116, 132
MboI GATC 4 cut(s) 3, 26, 114, 130
MboII GAAGA 1 cut(s) 308
MflI RGATCY 1 cut(s) 26
MluCI AATT 2 cut(s) 125, 186
MnlI CCTC 4 cut(s) 17, 38, 65, 211
MseI TTAA 1 cut(s) 59
MslI CAYNNNNRTG 2 cut(s) 138, 297
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 2 cut(s) 211, 255
MvaI CCWGG 1 cut(s) 91
MwoI GCNNNNNNNGC 1 cut(s) 218
NdeII GATC 4 cut(s) 3, 26, 114, 130
NlaIII CATG 2 cut(s) 137, 296
PagI TCATGA 1 cut(s) 292
PctI GAATGC 2 cut(s) 211, 255
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 2 cut(s) 211, 223
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PspPI GGNCC 1 cut(s) 198
PsuI RGATCY 1 cut(s) 26
RsaI GTAC 1 cut(s) 100
RsaNI GTAC 1 cut(s) 99
RseI CAYNNNNRTG 2 cut(s) 138, 297
SaqAI TTAA 1 cut(s) 59
SatI GCNGC 2 cut(s) 210, 222
Sau3AI GATC 4 cut(s) 3, 26, 114, 130
Sau96I GGNCC 1 cut(s) 198
ScrFI CCNGG 1 cut(s) 91
SetI ASST 3 cut(s) 104, 231, 335
SfaNI GCATC 1 cut(s) 289
SfcI CTRYAG 2 cut(s) 276, 334
SinI GGWCC 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 138, 297
Sse9I AATT 2 cut(s) 125, 186
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 89
TaaI ACNGT 1 cut(s) 246
TaiI ACGT 1 cut(s) 104
TasI AATT 2 cut(s) 125, 186
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 3 cut(s) 117, 251, 325
TseI GCWGC 2 cut(s) 209, 221
TspDTI ATGAA 4 cut(s) 156, 274, 281, 309
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 3 cut(s) 117, 251, 325
Van91I CCANNNNNTGG 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 68
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.