RLG00000011566

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004106
Physical Location & Seq
Forward (+)
10568138 .. 10568560
423 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011566

Sequence Viewer

Length: 423 bp
ATGGTGGTGGAAGGCGCAGGCAAGTCTTCAGCTTCGGAAGATCAGAGCAAGACGGAGTCGAACCCTAAATCAATGCAAGACGAGAACGAGCTCGAGGAAGGAGAGATTGTCAGCGGCGGCGAGGAGGAGTCTTTGAAGATCGCAAAGCCACTGCGCCACCCTTTGGAGCACTCATGGACCTTCTGGTTCGATAGCCCCTTGGCCAAGAACTCCAAGCACAAGCAAGAGGATTGGGGCAGCTCTATTCGCCTGATCTACACCTTCTCTACTGTTGAGGAATTCTGGGGTATATACAATAATATACGACATCCAAGCAAGTTGTCTGTGGGGACATACCTCAATTGTTTCAAAAACAAAATTGAGCCTAAGTGGGAGGATCCTATTTGTGCTAATGGAGGGAAATGGACTCTTAACTTTTACTAG

Protein Analysis

141

Amino Acids

16.03

Weight (kDa)

5.25

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 54 - 139 1.5e-28 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 163
AciI CCGC 2 cut(s) 114, 117
AclWI GGATC 2 cut(s) 371, 384
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 2 cut(s) 136, 349
AluBI AGCT 3 cut(s) 32, 91, 240
AluI AGCT 3 cut(s) 32, 91, 240
Alw21I GWGCWC 2 cut(s) 93, 171
AlwI GGATC 2 cut(s) 371, 384
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 1 cut(s) 237
ApoI RAATTY 1 cut(s) 278
AspLEI GCGC 2 cut(s) 17, 156
AspS9I GGNCC 1 cut(s) 177
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 1 cut(s) 177
BalI TGGCCA 1 cut(s) 203
BamHI GGATCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 93, 171
BbvI GCAGC 1 cut(s) 249
BfaI CTAG 1 cut(s) 421
BisI GCNGC 3 cut(s) 115, 118, 238
BlsI GCNGC 3 cut(s) 116, 119, 239
Bme18I GGWCC 1 cut(s) 177
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 177
BmiI GGNNCC 1 cut(s) 378
BpiI GAAGAC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 163
BseRI GAGGAG 2 cut(s) 137, 140
BseXI GCAGC 1 cut(s) 249
BshFI GGCC 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 93, 171
BsiHKCI CYCGRG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 93, 171
Bsp143I GATC 4 cut(s) 40, 138, 252, 376
BspACI CCGC 2 cut(s) 114, 117
BspANI GGCC 1 cut(s) 203
BspLI GGNNCC 1 cut(s) 378
BspPI GGATC 2 cut(s) 371, 384
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 4 cut(s) 40, 138, 252, 376
BssT1I CCWWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 307
BstHHI GCGC 2 cut(s) 17, 156
BstKTI GATC 4 cut(s) 43, 141, 255, 379
BstMBI GATC 4 cut(s) 40, 138, 252, 376
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstV1I GCAGC 1 cut(s) 249
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 2 cut(s) 17, 156
Cfr13I GGNCC 1 cut(s) 177
CviAII CATG 1 cut(s) 174
CviJI RGCY 7 cut(s) 32, 91, 148, 195, 203, 240, 364
CviKI_1 RGCY 7 cut(s) 32, 91, 148, 195, 203, 240, 364
DdeI CTNAG 1 cut(s) 366
DpnI GATC 4 cut(s) 42, 140, 254, 378
DpnII GATC 4 cut(s) 40, 138, 252, 376
EaeI YGGCCR 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 1 cut(s) 177
FaiI YATR 5 cut(s) 175, 290, 292, 302, 334
FaqI GGGAC 1 cut(s) 343
FatI CATG 1 cut(s) 173
Fnu4HI GCNGC 3 cut(s) 115, 118, 238
FokI GGATG 1 cut(s) 294
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 3 cut(s) 115, 118, 238
FspBI CTAG 1 cut(s) 421
GlaI GCGC 2 cut(s) 16, 155
GluI GCNGC 3 cut(s) 115, 118, 238
HaeIII GGCC 1 cut(s) 203
HhaI GCGC 2 cut(s) 17, 156
Hin1II CATG 1 cut(s) 177
Hin6I GCGC 2 cut(s) 15, 154
HinP1I GCGC 2 cut(s) 15, 154
HinfI GANTC 3 cut(s) 56, 128, 406
Hpy188I TCNGA 2 cut(s) 37, 45
HpyAV CCTTC 4 cut(s) 5, 92, 190, 271
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 366
Hsp92II CATG 1 cut(s) 177
HspAI GCGC 2 cut(s) 15, 154
Kzo9I GATC 4 cut(s) 40, 138, 252, 376
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 4 cut(s) 3, 169, 263, 268
Lsp1109I GCAGC 1 cut(s) 249
MaeI CTAG 1 cut(s) 421
MalI GATC 4 cut(s) 42, 140, 254, 378
MboI GATC 4 cut(s) 40, 138, 252, 376
MboII GAAGA 3 cut(s) 18, 50, 148
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 2 cut(s) 93, 171
MlsI TGGCCA 1 cut(s) 203
MluCI AATT 3 cut(s) 278, 340, 357
MluNI TGGCCA 1 cut(s) 203
MlyI GAGTC 3 cut(s) 65, 137, 400
MnlI CCTC 8 cut(s) 88, 115, 118, 220, 268, 347, 367, 389
Mox20I TGGCCA 1 cut(s) 203
MscI TGGCCA 1 cut(s) 203
MseI TTAA 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 114
MunI CAATTG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 4 cut(s) 40, 138, 252, 376
NlaIII CATG 1 cut(s) 177
NlaIV GGNNCC 1 cut(s) 378
PaeR7I CTCGAG 1 cut(s) 92
PflFI GACNNNGTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 3 cut(s) 116, 119, 239
PleI GAGTC 3 cut(s) 64, 136, 400
PpsI GAGTC 3 cut(s) 64, 136, 400
Psp124BI GAGCTC 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 177
PspXI VCTCGAGB 1 cut(s) 92
PsuI RGATCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 3 cut(s) 115, 118, 238
Sau3AI GATC 4 cut(s) 40, 138, 252, 376
Sau96I GGNCC 1 cut(s) 177
SchI GAGTC 3 cut(s) 65, 137, 400
SduI GDGCHC 2 cut(s) 93, 171
SetI ASST 6 cut(s) 34, 93, 182, 242, 263, 339
Sfr274I CTCGAG 1 cut(s) 92
SinI GGWCC 1 cut(s) 177
SlaI CTCGAG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 3 cut(s) 278, 340, 357
SsiI CCGC 2 cut(s) 114, 117
SspMI CTAG 1 cut(s) 421
SstI GAGCTC 1 cut(s) 93
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 3 cut(s) 59, 93, 189
TasI AATT 3 cut(s) 278, 340, 357
TauI GCSGC 2 cut(s) 117, 120
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 1 cut(s) 156
TseI GCWGC 1 cut(s) 237
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 1 cut(s) 156
Tth111I GACNNNGTC 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 177
XapI RAATTY 1 cut(s) 278
XhoI CTCGAG 1 cut(s) 92
XspI CTAG 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.