pycom05g26930

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004106
Physical Location & Seq
Reverse (-)
28052105 .. 28054610
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g26930.1

Sequence Viewer

Length: 723 bp
ATGCAAACCGTAACAATGGTGGTCGAAGACGCACCCAAGACTTCAGCTTCCGAAGATCAGGCCAAGACCGAGGCGCACCCTATGCCGAGGGGGGAGGACGACGACGAGACCGAAGAAGGAGAGATCGTTGGCGATCAAGATTCGGCTTCTTCGAAGCCGTCGAAAGGAATAGCGCCGCAGTCACACCCTCTGGAGCACTCCTGGACCTTCTGGTTCGATAACCCCGCCGCCAAGTCCGCCAAGCTCAAGCAGGCGGATTGGGGCAGCACCATCCACCCGATCTACACCTTCTCTACCGTCGAGGAGTTCTGGAGCATTTACAACAATATACGTCGTCCGAGCAATTTGAATATGGGGACCGATTTCCATTGTTTCAAATACAAAATTGAGCCAAAGTGGGAGGATCCTGTTTGTGCTAATGGAGGAAAGTGGACTGTCACTTATCCTAAAGGGAAATCTGATACCCCTTGGTTGTATACGTTGCTAGCAATGATAGGAGAACAGTTTGATCATGGAGATGAAATCTGTGGAGCAGTTGTCAATGTCAGAACTAGGCAAGACAAAATAGCTCTTTGGACCAAGAATGCAGCAAATGAAGCTGCTCAGCTGAGCATTGGGAAACAATGGAAGGGGTTTCTGGATTGCAATGAAACTATTGGATTCATATTTCATGAGGATGCGATGAGACAGGAGAGAGCTGCCAAGAATAAATACGTAGCCTGA

Protein Analysis

241

Amino Acids

27.07

Weight (kDa)

5.2

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 63 - 218 6.5e-57 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 476
AciI CCGC 5 cut(s) 176, 225, 228, 237, 254
AclWI GGATC 2 cut(s) 398, 411
AcuI CTGAAG 1 cut(s) 27
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 2 cut(s) 349, 376
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 6 cut(s) 47, 244, 569, 599, 607, 698
AluI AGCT 6 cut(s) 47, 244, 569, 599, 607, 698
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 101, 679
AlwI GGATC 2 cut(s) 398, 411
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 4 cut(s) 264, 587, 599, 698
AspLEI GCGC 2 cut(s) 76, 175
AspS9I GGNCC 3 cut(s) 204, 357, 576
AsuII TTCGAA 1 cut(s) 152
AsuNHI GCTAGC 1 cut(s) 484
AvaII GGWCC 3 cut(s) 204, 357, 576
BamHI GGATCC 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 4 cut(s) 276, 586, 599, 685
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 1 cut(s) 202
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 2 cut(s) 101, 679
BfaI CTAG 2 cut(s) 485, 552
BfoI RGCGCY 1 cut(s) 176
BisI GCNGC 6 cut(s) 176, 228, 265, 588, 600, 699
BlpI GCTNAGC 2 cut(s) 603, 608
BlsI GCNGC 6 cut(s) 177, 229, 266, 589, 601, 700
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 3 cut(s) 204, 357, 576
BmgT120I GGNCC 3 cut(s) 204, 357, 576
BmiI GGNNCC 2 cut(s) 358, 405
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 1 cut(s) 667
BmtI GCTAGC 1 cut(s) 488
BpiI GAAGAC 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 212, 331
Bpu1102I GCTNAGC 2 cut(s) 603, 608
Bpu14I TTCGAA 1 cut(s) 152
BpuEI CTTGAG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 715
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 3 cut(s) 69, 86, 467
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 522, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse3DI GCAATG 2 cut(s) 495, 652
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 3 cut(s) 69, 86, 467
BseGI GGATG 2 cut(s) 270, 682
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 2 cut(s) 495, 652
BseMII CTCAG 2 cut(s) 599, 617
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 4 cut(s) 276, 586, 599, 685
BshFI GGCC 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 198
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 2 cut(s) 101, 679
BsmFI GGGAC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 1 cut(s) 62
Bso31I GGTCTC 1 cut(s) 101
Bsp119I TTCGAA 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 6 cut(s) 55, 123, 133, 279, 403, 508
Bsp1720I GCTNAGC 2 cut(s) 603, 608
BspACI CCGC 5 cut(s) 176, 225, 228, 237, 254
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 2 cut(s) 600, 616
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 2 cut(s) 358, 405
BspOI GCTAGC 1 cut(s) 488
BspPI GGATC 2 cut(s) 398, 411
BspT104I TTCGAA 1 cut(s) 152
BspTNI GGTCTC 1 cut(s) 101
BsrDI GCAATG 2 cut(s) 495, 652
BssECI CCNNGG 3 cut(s) 69, 86, 467
BssMI GATC 6 cut(s) 55, 123, 133, 279, 403, 508
BssNAI GTATAC 1 cut(s) 477
BssT1I CCWWGG 1 cut(s) 467
Bst1107I GTATAC 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 4 cut(s) 10, 298, 436, 504
BstAPI GCANNNNNTGC 1 cut(s) 82
BstBAI YACGTR 1 cut(s) 715
BstBI TTCGAA 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 252, 486
BstDEI CTNAG 2 cut(s) 603, 608
BstF5I GGATG 2 cut(s) 270, 682
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 76, 175
BstKTI GATC 6 cut(s) 58, 126, 136, 282, 406, 511
BstMAI GTCTC 2 cut(s) 101, 679
BstMBI GATC 6 cut(s) 55, 123, 133, 279, 403, 508
BstMWI GCNNNNNNNGC 3 cut(s) 82, 236, 596
BstNI CCWGG 1 cut(s) 202
BstSCI CCNGG 1 cut(s) 200
BstSNI TACGTA 1 cut(s) 715
BstV1I GCAGC 4 cut(s) 276, 586, 599, 685
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BstZ17I GTATAC 1 cut(s) 477
BsuRI GGCC 1 cut(s) 62
BtgZI GCGATG 1 cut(s) 695
BtsCI GGATG 2 cut(s) 270, 682
Cac8I GCNNGC 2 cut(s) 252, 486
CciI TCATGA 1 cut(s) 670
CfoI GCGC 2 cut(s) 76, 175
Cfr13I GGNCC 3 cut(s) 204, 357, 576
CseI GACGC 1 cut(s) 38
CviAII CATG 2 cut(s) 512, 671
DdeI CTNAG 2 cut(s) 603, 608
DpnI GATC 6 cut(s) 57, 125, 135, 281, 405, 510
DpnII GATC 6 cut(s) 55, 123, 133, 279, 403, 508
EciI GGCGGA 2 cut(s) 226, 269
Eco105I TACGTA 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 101
Eco47I GGWCC 3 cut(s) 204, 357, 576
Eco57I CTGAAG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 467
ErhI CCWWGG 1 cut(s) 467
FaeI CATG 2 cut(s) 515, 674
FaiI YATR 7 cut(s) 83, 329, 353, 477, 513, 665, 672
FaqI GGGAC 1 cut(s) 370
FatI CATG 2 cut(s) 511, 670
FauI CCCGC 1 cut(s) 232
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 6 cut(s) 176, 228, 265, 588, 600, 699
FokI GGATG 2 cut(s) 257, 689
Fsp4HI GCNGC 6 cut(s) 176, 228, 265, 588, 600, 699
FspBI CTAG 2 cut(s) 485, 552
GlaI GCGC 2 cut(s) 75, 174
GluI GCNGC 6 cut(s) 176, 228, 265, 588, 600, 699
GsuI CTGGAG 2 cut(s) 212, 331
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 62
HgaI GACGC 1 cut(s) 38
HhaI GCGC 2 cut(s) 76, 175
Hin1II CATG 2 cut(s) 515, 674
Hin6I GCGC 2 cut(s) 74, 173
HinP1I GCGC 2 cut(s) 74, 173
HinfI GANTC 2 cut(s) 140, 660
Hpy166II GTNNAC 2 cut(s) 432, 477
Hpy188I TCNGA 4 cut(s) 52, 339, 460, 548
Hpy188III TCNNGA 5 cut(s) 137, 191, 310, 638, 671
Hpy8I GTNNAC 2 cut(s) 432, 477
Hpy99I CGWCG 5 cut(s) 104, 107, 163, 302, 336
HpyAV CCTTC 4 cut(s) 110, 217, 298, 622
HpyCH4III ACNGT 4 cut(s) 10, 298, 436, 504
HpyCH4IV ACGT 3 cut(s) 331, 479, 714
HpyCH4V TGCA 3 cut(s) 4, 587, 645
HpyF10VI GCNNNNNNNGC 3 cut(s) 82, 236, 596
HpyF3I CTNAG 2 cut(s) 603, 608
HpySE526I ACGT 3 cut(s) 331, 479, 714
Hsp92II CATG 2 cut(s) 515, 674
HspAI GCGC 2 cut(s) 74, 173
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 6 cut(s) 55, 123, 133, 279, 403, 508
LmnI GCTCC 3 cut(s) 193, 312, 530
Lsp1109I GCAGC 4 cut(s) 276, 586, 599, 685
LweI GCATC 1 cut(s) 667
MaeI CTAG 2 cut(s) 485, 552
MaeII ACGT 3 cut(s) 331, 479, 714
MaeIII GTNAC 3 cut(s) 10, 180, 436
MalI GATC 6 cut(s) 57, 125, 135, 281, 405, 510
MboI GATC 6 cut(s) 55, 123, 133, 279, 403, 508
MboII GAAGA 4 cut(s) 38, 65, 125, 141
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 2 cut(s) 343, 384
MnlI CCTC 8 cut(s) 64, 81, 88, 198, 295, 394, 416, 667
MslI CAYNNNNRTG 2 cut(s) 516, 675
MspA1I CMGCKG 1 cut(s) 607
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 1 cut(s) 202
MwoI GCNNNNNNNGC 3 cut(s) 82, 236, 596
NdeII GATC 6 cut(s) 55, 123, 133, 279, 403, 508
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 2 cut(s) 515, 674
NlaIV GGNNCC 2 cut(s) 358, 405
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 180, 436
NspV TTCGAA 1 cut(s) 152
PagI TCATGA 1 cut(s) 670
PcsI WCGNNNNNNNCGW 3 cut(s) 108, 149, 158
PctI GAATGC 1 cut(s) 589
PfeI GAWTC 2 cut(s) 140, 660
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 6 cut(s) 177, 229, 266, 589, 601, 700
Ppu21I YACGTR 1 cut(s) 715
Psp6I CCWGG 1 cut(s) 200
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 358, 405
PspPI GGNCC 3 cut(s) 204, 357, 576
PsuI RGATCY 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 607
RseI CAYNNNNRTG 2 cut(s) 516, 675
SatI GCNGC 6 cut(s) 176, 228, 265, 588, 600, 699
Sau3AI GATC 6 cut(s) 55, 123, 133, 279, 403, 508
Sau96I GGNCC 3 cut(s) 204, 357, 576
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 1 cut(s) 667
SfuI TTCGAA 1 cut(s) 152
SinI GGWCC 3 cut(s) 204, 357, 576
SmiMI CAYNNNNRTG 2 cut(s) 516, 675
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SnaBI TACGTA 1 cut(s) 715
Sse9I AATT 2 cut(s) 343, 384
SsiI CCGC 5 cut(s) 176, 225, 228, 237, 254
SspMI CTAG 2 cut(s) 485, 552
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 1 cut(s) 467
TaaI ACNGT 4 cut(s) 10, 298, 436, 504
TaiI ACGT 3 cut(s) 334, 482, 717
TaqI TCGA 5 cut(s) 24, 152, 161, 216, 300
TaqII GACCGA 3 cut(s) 83, 125, 374
TasI AATT 2 cut(s) 343, 384
TauI GCSGC 2 cut(s) 178, 230
TfiI GAWTC 2 cut(s) 140, 660
TseFI GTSAC 2 cut(s) 180, 436
TseI GCWGC 4 cut(s) 264, 587, 599, 698
Tsp45I GTSAC 2 cut(s) 180, 436
TspDTI ATGAA 5 cut(s) 534, 609, 652, 659, 663
VpaK11BI GGWCC 3 cut(s) 204, 357, 576
XmiI GTMKAC 1 cut(s) 476
XspI CTAG 2 cut(s) 485, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.