RLG00000031930

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004106
Physical Location & Seq
Forward (+)
9602470 .. 9605305
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031930

Sequence Viewer

Length: 708 bp
ATGTTAAGCAAAGCTAACGCTGGAACTAATATCAGTGATGGAGACCACTTGGATGGAGATTATGATCATATTGACGAATTCACCTATCCAAGACTTGCCCAACCTTCTTCAGACCCTCGAACATGGTGGTCTAGCGCCAAATACACCTTCGCAAACTTTGGGAGACGACACCCAAATTTGAAGGAAGGTTTTGAACTGGTTATAACAGTCGTTGGGCATCCTTACAACATCATTCTTAAGATCCCCAGTATGGGCAATACTCTTGATTGGCTACAGACTTACTCCGACGAGGAACATAAAAAAAGGACTTTCATCTCCGTGGCGGCGTATACACTGCAAGCACTAATCGACGGTAAGGTTCTGAGCCTGGTAGATGGAATCTCTTTCATCATAAAGCAAGCACTAAAACGACACTCAGCACCGGCAGGCAAGCTGGCCGATAAGAACTTGCTAGCACTGATCGGGGAACATTTTGATCATGGAGATGAAATATGTGGAGCAGTTGTCAATGTCAGAAATAGGCAAGAAAAAATTGCTGTTTGGACCAAGAATGCACAAATGAGATTGCTCAGCATTGGAAAACAGTGGAAGGGCTTTCTGGAGAGCAACGAAACTGTAGGGTTCAGGTTTCATGAAGATACAAGGCGCGATGGAGCAGCGCTAAGAATAGATACGCAGTGTAATCTCTATCTTGCTCTGTGCCAGTAA

Protein Analysis

236

Amino Acids

26.5

Weight (kDa)

7.12

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 140 - 205 2.5e-14 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccI GTMKAC 1 cut(s) 329
AccII CGCG 1 cut(s) 648
AciI CCGC 1 cut(s) 323
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 1 cut(s) 435
AcsI RAATTY 2 cut(s) 77, 175
AcuI CTGAAG 1 cut(s) 93
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 2 cut(s) 250, 251
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 2 cut(s) 181, 194
AjnI CCWGG 1 cut(s) 366
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 2 cut(s) 14, 433
AluI AGCT 2 cut(s) 14, 433
Alw26I GTCTC 2 cut(s) 36, 157
AlwI GGATC 1 cut(s) 235
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 2 cut(s) 77, 175
AspLEI GCGC 3 cut(s) 137, 648, 661
AspS9I GGNCC 1 cut(s) 543
AsuHPI GGTGA 1 cut(s) 73
AsuNHI GCTAGC 1 cut(s) 451
AvaII GGWCC 1 cut(s) 543
BbvI GCAGC 1 cut(s) 668
BccI CCATC 4 cut(s) 32, 47, 368, 644
BciT130I CCWGG 1 cut(s) 368
BclI TGATCA 2 cut(s) 64, 475
BcoDI GTCTC 2 cut(s) 36, 157
BfaI CTAG 2 cut(s) 132, 452
BfmI CTRYAG 2 cut(s) 272, 615
BfoI RGCGCY 2 cut(s) 138, 662
BfrI CTTAAG 1 cut(s) 236
BisI GCNGC 2 cut(s) 324, 657
BlpI GCTNAGC 1 cut(s) 569
BlsI GCNGC 2 cut(s) 325, 658
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 543
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 240
BmsI GCATC 1 cut(s) 226
BmtI GCTAGC 1 cut(s) 455
BmuI ACTGGG 1 cut(s) 240
BpmI CTGGAG 1 cut(s) 620
Bpu1102I GCTNAGC 1 cut(s) 569
BsaBI GATNNNNATC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 318
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 250, 251
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 3 cut(s) 201, 246, 703
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 2 cut(s) 58, 217
BseJI GATNNNNATC 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 250, 251
BseMII CTCAG 3 cut(s) 353, 429, 583
BseNI ACTGG 3 cut(s) 201, 246, 703
BseXI GCAGC 1 cut(s) 668
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 422
BslI CCNNNNNNNGG 2 cut(s) 250, 251
BsmAI GTCTC 2 cut(s) 36, 157
BsmBI CGTCTC 1 cut(s) 157
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 1 cut(s) 437
Bso31I GGTCTC 1 cut(s) 36
Bsp143I GATC 4 cut(s) 64, 240, 459, 475
Bsp1720I GCTNAGC 1 cut(s) 569
BspACI CCGC 1 cut(s) 323
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 3 cut(s) 354, 428, 582
BspFNI CGCG 1 cut(s) 648
BspHI TCATGA 1 cut(s) 631
BspOI GCTAGC 1 cut(s) 455
BspPI GGATC 1 cut(s) 235
BspTI CTTAAG 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 36
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 3 cut(s) 201, 246, 703
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 4 cut(s) 64, 240, 459, 475
BssNAI GTATAC 1 cut(s) 330
Bst1107I GTATAC 1 cut(s) 330
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 4 cut(s) 208, 353, 585, 616
BstAFI CTTAAG 1 cut(s) 236
BstC8I GCNNGC 6 cut(s) 339, 399, 427, 431, 435, 453
BstDEI CTNAG 4 cut(s) 362, 415, 569, 662
BstDSI CCRYGG 1 cut(s) 318
BstF5I GGATG 2 cut(s) 58, 217
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 2 cut(s) 138, 662
BstHHI GCGC 3 cut(s) 137, 648, 661
BstKTI GATC 4 cut(s) 67, 243, 462, 478
BstMAI GTCTC 2 cut(s) 36, 157
BstMBI GATC 4 cut(s) 64, 240, 459, 475
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 2 cut(s) 272, 615
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 1 cut(s) 668
BstX2I RGATCY 1 cut(s) 240
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 1 cut(s) 240
BstZ17I GTATAC 1 cut(s) 330
BsuRI GGCC 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 318
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 2 cut(s) 58, 217
BtsI GCAGTG 2 cut(s) 332, 683
BtsIMutI CAGTG 5 cut(s) 40, 332, 455, 590, 683
Cac8I GCNNGC 6 cut(s) 339, 399, 427, 431, 435, 453
CciI TCATGA 1 cut(s) 631
CfoI GCGC 3 cut(s) 137, 648, 661
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 543
CviAII CATG 3 cut(s) 123, 479, 632
CviJI RGCY 6 cut(s) 14, 271, 366, 433, 437, 594
CviKI_1 RGCY 6 cut(s) 14, 271, 366, 433, 437, 594
DdeI CTNAG 4 cut(s) 362, 415, 569, 662
DpnI GATC 4 cut(s) 66, 242, 461, 477
DpnII GATC 4 cut(s) 64, 240, 459, 475
EaeI YGGCCR 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 543
Eco47III AGCGCT 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 93
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 3 cut(s) 126, 482, 635
FatI CATG 3 cut(s) 122, 478, 631
FbaI TGATCA 2 cut(s) 64, 475
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 2 cut(s) 324, 657
FokI GGATG 2 cut(s) 65, 204
Fsp4HI GCNGC 2 cut(s) 324, 657
FspBI CTAG 2 cut(s) 132, 452
GlaI GCGC 3 cut(s) 136, 647, 660
GluI GCNGC 2 cut(s) 324, 657
GsuI CTGGAG 1 cut(s) 620
HaeII RGCGCY 2 cut(s) 138, 662
HaeIII GGCC 1 cut(s) 437
HapII CCGG 1 cut(s) 422
HhaI GCGC 3 cut(s) 137, 648, 661
Hin1II CATG 3 cut(s) 126, 482, 635
Hin6I GCGC 3 cut(s) 135, 646, 659
HinP1I GCGC 3 cut(s) 135, 646, 659
HinfI GANTC 1 cut(s) 378
HpaII CCGG 1 cut(s) 422
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 4 cut(s) 112, 286, 363, 515
Hpy188III TCNNGA 3 cut(s) 263, 599, 632
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 2 cut(s) 290, 353
HpyAV CCTTC 5 cut(s) 114, 157, 175, 179, 583
HpyCH4III ACNGT 4 cut(s) 208, 353, 585, 616
HpyCH4V TGCA 2 cut(s) 337, 554
HpyF3I CTNAG 4 cut(s) 362, 415, 569, 662
Hsp92II CATG 3 cut(s) 126, 482, 635
HspAI GCGC 3 cut(s) 135, 646, 659
Ksp22I TGATCA 2 cut(s) 64, 475
Kzo9I GATC 4 cut(s) 64, 240, 459, 475
LmnI GCTCC 2 cut(s) 497, 653
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 1 cut(s) 226
MaeI CTAG 2 cut(s) 132, 452
MalI GATC 4 cut(s) 66, 242, 461, 477
MboI GATC 4 cut(s) 64, 240, 459, 475
MboII GAAGA 2 cut(s) 99, 647
MflI RGATCY 1 cut(s) 240
MluCI AATT 3 cut(s) 77, 175, 531
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 2 cut(s) 126, 283
MseI TTAA 2 cut(s) 5, 237
MslI CAYNNNNRTG 3 cut(s) 51, 317, 483
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 368
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 1 cut(s) 368
MvnI CGCG 1 cut(s) 648
NdeII GATC 4 cut(s) 64, 240, 459, 475
NheI GCTAGC 1 cut(s) 451
NlaIII CATG 3 cut(s) 126, 482, 635
PagI TCATGA 1 cut(s) 631
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 1 cut(s) 378
PkrI GCNGC 2 cut(s) 325, 658
PsiI TTATAA 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspPI GGNCC 1 cut(s) 543
PsuI RGATCY 1 cut(s) 240
RseI CAYNNNNRTG 3 cut(s) 51, 317, 483
SaqAI TTAA 2 cut(s) 5, 237
SatI GCNGC 2 cut(s) 324, 657
Sau3AI GATC 4 cut(s) 64, 240, 459, 475
Sau96I GGNCC 1 cut(s) 543
ScrFI CCNGG 1 cut(s) 368
SetI ASST 8 cut(s) 16, 86, 106, 149, 190, 360, 435, 629
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 2 cut(s) 272, 615
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 3 cut(s) 51, 317, 483
SmlI CTYRAG 1 cut(s) 236
SmoI CTYRAG 1 cut(s) 236
Sse9I AATT 3 cut(s) 77, 175, 531
SsiI CCGC 1 cut(s) 323
SspMI CTAG 2 cut(s) 132, 452
StyD4I CCNGG 1 cut(s) 366
TaaI ACNGT 4 cut(s) 208, 353, 585, 616
TaqI TCGA 2 cut(s) 118, 348
TasI AATT 3 cut(s) 77, 175, 531
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 2 cut(s) 5, 237
Tru9I TTAA 2 cut(s) 5, 237
TscAI CASTG 5 cut(s) 40, 339, 462, 590, 683
TseI GCWGC 1 cut(s) 656
TspDTI ATGAA 5 cut(s) 301, 376, 501, 620, 648
TspGWI ACGGA 1 cut(s) 307
TspRI CASTG 5 cut(s) 40, 339, 462, 590, 683
Vha464I CTTAAG 1 cut(s) 236
VpaK11BI GGWCC 1 cut(s) 543
XapI RAATTY 2 cut(s) 77, 175
XmiI GTMKAC 1 cut(s) 329
XspI CTAG 2 cut(s) 132, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.