MD10G1268700.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004106
Physical Location & Seq
Reverse (-)
36149899 .. 36152430
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1268700.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGTGGTCGAGGATGCACCCAAGACTTCAGCATCAGAAGATCAGGCCAAGACCGAGGCGAACGCTAAGCCGAGGGAGGAGGACGACGAGCCCGAAGAAGGAGAGATCGTTGGCGACCAAGACTCGGCGTCGTCGAAGCCTTCGAAGGGAATTGCGCCGCAGTCTCACGCCCTGGAGCACTCCTGGACCTTCTCGTTAAAAAGCCCTGCCGCAAAGTCCGTCAAGTCCAAGCAGGAGCATTGGGGCAGCTCCATCCGCCCGATCTACACCTTCACTACCGTCGAGGAGTTCTGGAGCATTTACAACAAAATAGGTCATCCTAGCAAGTTCAATGCGGGGACGGATTTCCATTGTTTCAAATACAAAATTGAGCCAAAGTGGGAGGATCCTGTGTGTGCTAATGGAGGAAAGTGGACTGTCACCTATCCTAAAGGGAAATCTGATACCTCTTGGTTGTATACGTTGCTAGCAATGATAGGAGAACAGTTTGATCATGGAGATGAAATCTGTGGAGCAGTTGTCAGTGTCAGAAAAAGCCTAGAAAAAATAACTCTTTGGACCAAGAATGCAACAAATGAAGCTGCTCAGGTGAGCATTGGGAAACAATGGAAGGGGTTTCTGGACTACAATGAAACTATTGGGTTCATATTTCATGAGGATGCGAATAGACTTGGGAGAGGTGCCAAGAATAAATACGTAGCCTGA

Protein Analysis

235

Amino Acids

26.13

Weight (kDa)

5.84

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 58 - 212 7.1e-52 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 458
AciI CCGC 4 cut(s) 158, 210, 256, 335
AclWI GGATC 2 cut(s) 380, 393
AcuI CTGAAG 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 128
AfiI CCNNNNNNNGG 3 cut(s) 98, 124, 146
AgsI TTSAA 2 cut(s) 331, 358
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 2 cut(s) 171, 182
AluBI AGCT 2 cut(s) 249, 581
AluI AGCT 2 cut(s) 249, 581
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 2 cut(s) 380, 393
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 2 cut(s) 246, 581
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 186, 558
AsuHPI GGTGA 2 cut(s) 412, 601
AsuII TTCGAA 1 cut(s) 143
AsuNHI GCTAGC 1 cut(s) 466
AvaII GGWCC 2 cut(s) 186, 558
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 258, 568
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 2 cut(s) 173, 184
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 3 cut(s) 321, 467, 539
BisI GCNGC 4 cut(s) 158, 210, 247, 582
BlpI GCTNAGC 1 cut(s) 66
BlsI GCNGC 4 cut(s) 159, 211, 248, 583
Bme1390I CCNGG 2 cut(s) 173, 184
Bme18I GGWCC 2 cut(s) 186, 558
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 186, 558
BmiI GGNNCC 2 cut(s) 387, 682
BmrFI CCNGG 2 cut(s) 173, 184
BmsI GCATC 3 cut(s) 4, 41, 649
BmtI GCTAGC 1 cut(s) 470
BpmI CTGGAG 2 cut(s) 194, 313
Bpu10I CCTNAGC 1 cut(s) 585
Bpu1102I GCTNAGC 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 143
BsaAI YACGTR 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 54, 71, 171
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 124, 146
Bse3DI GCAATG 1 cut(s) 477
BseBI CCWGG 2 cut(s) 173, 184
BseDI CCNNGG 3 cut(s) 54, 71, 171
BseGI GGATG 4 cut(s) 19, 252, 316, 664
BseLI CCNNNNNNNGG 3 cut(s) 98, 124, 146
BseMI GCAATG 1 cut(s) 477
BseMII CTCAG 1 cut(s) 599
BseRI GAGGAG 2 cut(s) 92, 299
BseXI GCAGC 2 cut(s) 258, 568
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 3 cut(s) 98, 124, 146
BsmAI GTCTC 1 cut(s) 168
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 93, 180
Bsp143I GATC 5 cut(s) 40, 105, 261, 385, 490
Bsp1720I GCTNAGC 1 cut(s) 66
BspACI CCGC 4 cut(s) 158, 210, 256, 335
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 1 cut(s) 598
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 387, 682
BspOI GCTAGC 1 cut(s) 470
BspPI GGATC 2 cut(s) 380, 393
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 680
BsrDI GCAATG 1 cut(s) 477
BssECI CCNNGG 3 cut(s) 54, 71, 171
BssMI GATC 5 cut(s) 40, 105, 261, 385, 490
BssNAI GTATAC 1 cut(s) 459
BssNI GRCGYC 1 cut(s) 128
Bst1107I GTATAC 1 cut(s) 459
Bst2UI CCWGG 2 cut(s) 173, 184
Bst4CI ACNGT 3 cut(s) 280, 418, 486
BstACI GRCGYC 1 cut(s) 128
BstBAI YACGTR 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 468
BstDEI CTNAG 2 cut(s) 66, 585
BstF5I GGATG 4 cut(s) 19, 252, 316, 664
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 5 cut(s) 43, 108, 264, 388, 493
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 5 cut(s) 40, 105, 261, 385, 490
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstNI CCWGG 2 cut(s) 173, 184
BstSCI CCNGG 2 cut(s) 171, 182
BstSNI TACGTA 1 cut(s) 697
BstV1I GCAGC 2 cut(s) 258, 568
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 47
BtsCI GGATG 4 cut(s) 19, 252, 316, 664
BtsIMutI CAGTG 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 468
CciI TCATGA 1 cut(s) 652
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 186, 558
CseI GACGC 1 cut(s) 117
CviAII CATG 2 cut(s) 494, 653
DdeI CTNAG 2 cut(s) 66, 585
DpnI GATC 5 cut(s) 42, 107, 263, 387, 492
DpnII GATC 5 cut(s) 40, 105, 261, 385, 490
DriI GACNNNNNGTC 1 cut(s) 127
Eam1105I GACNNNNNGTC 1 cut(s) 127
EciI GGCGGA 1 cut(s) 245
Eco105I TACGTA 1 cut(s) 697
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 186, 558
Eco57I CTGAAG 1 cut(s) 12
EcoRII CCWGG 2 cut(s) 171, 182
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 2 cut(s) 497, 656
FaiI YATR 4 cut(s) 459, 495, 647, 654
FaqI GGGAC 1 cut(s) 352
FatI CATG 2 cut(s) 493, 652
FauI CCCGC 1 cut(s) 328
FbaI TGATCA 1 cut(s) 490
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 4 cut(s) 158, 210, 247, 582
FokI GGATG 4 cut(s) 26, 239, 303, 671
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 4 cut(s) 158, 210, 247, 582
FspBI CTAG 3 cut(s) 321, 467, 539
GlaI GCGC 1 cut(s) 156
GluI GCNGC 4 cut(s) 158, 210, 247, 582
GsuI CTGGAG 2 cut(s) 194, 313
HaeIII GGCC 1 cut(s) 47
HgaI GACGC 1 cut(s) 117
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 2 cut(s) 497, 656
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 1 cut(s) 122
HphI GGTGA 2 cut(s) 412, 601
Hpy166II GTNNAC 2 cut(s) 414, 459
Hpy188I TCNGA 3 cut(s) 37, 442, 530
Hpy188III TCNNGA 3 cut(s) 292, 620, 653
Hpy8I GTNNAC 2 cut(s) 414, 459
Hpy99I CGWCG 4 cut(s) 89, 133, 136, 284
HpyAV CCTTC 6 cut(s) 92, 139, 150, 199, 280, 604
HpyCH4III ACNGT 3 cut(s) 280, 418, 486
HpyCH4IV ACGT 2 cut(s) 461, 696
HpyCH4V TGCA 2 cut(s) 17, 569
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpyF3I CTNAG 2 cut(s) 66, 585
HpySE526I ACGT 2 cut(s) 461, 696
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 2 cut(s) 497, 656
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 5 cut(s) 40, 105, 261, 385, 490
LmnI GCTCC 5 cut(s) 175, 235, 254, 294, 512
Lsp1109I GCAGC 2 cut(s) 258, 568
LweI GCATC 3 cut(s) 4, 41, 649
MaeI CTAG 3 cut(s) 321, 467, 539
MaeII ACGT 2 cut(s) 461, 696
MaeIII GTNAC 1 cut(s) 418
MalI GATC 5 cut(s) 42, 107, 263, 387, 492
MboI GATC 5 cut(s) 40, 105, 261, 385, 490
MboII GAAGA 2 cut(s) 50, 107
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 93, 180
MluCI AATT 2 cut(s) 150, 366
MlyI GAGTC 1 cut(s) 116
MseI TTAA 1 cut(s) 197
MslI CAYNNNNRTG 2 cut(s) 498, 657
MspR9I CCNGG 2 cut(s) 173, 184
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 2 cut(s) 173, 184
MwoI GCNNNNNNNGC 1 cut(s) 255
NdeII GATC 5 cut(s) 40, 105, 261, 385, 490
NheI GCTAGC 1 cut(s) 466
NlaIII CATG 2 cut(s) 497, 656
NlaIV GGNNCC 2 cut(s) 387, 682
NmeAIII GCCGAG 2 cut(s) 96, 104
NmuCI GTSAC 1 cut(s) 418
NspV TTCGAA 1 cut(s) 143
PagI TCATGA 1 cut(s) 652
PcsI WCGNNNNNNNCGW 3 cut(s) 90, 131, 140
PctI GAATGC 1 cut(s) 571
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 4 cut(s) 159, 211, 248, 583
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 697
Psp6I CCWGG 2 cut(s) 171, 182
PspGI CCWGG 2 cut(s) 171, 182
PspN4I GGNNCC 2 cut(s) 387, 682
PspPI GGNCC 2 cut(s) 186, 558
PsuI RGATCY 1 cut(s) 385
RseI CAYNNNNRTG 2 cut(s) 498, 657
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 4 cut(s) 158, 210, 247, 582
Sau3AI GATC 5 cut(s) 40, 105, 261, 385, 490
Sau96I GGNCC 2 cut(s) 186, 558
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 2 cut(s) 173, 184
SduI GDGCHC 2 cut(s) 93, 180
SfaNI GCATC 3 cut(s) 4, 41, 649
SfuI TTCGAA 1 cut(s) 143
SinI GGWCC 2 cut(s) 186, 558
SmiMI CAYNNNNRTG 2 cut(s) 498, 657
SnaBI TACGTA 1 cut(s) 697
Sse9I AATT 2 cut(s) 150, 366
SsiI CCGC 4 cut(s) 158, 210, 256, 335
SspMI CTAG 3 cut(s) 321, 467, 539
StyD4I CCNGG 2 cut(s) 171, 182
TaaI ACNGT 3 cut(s) 280, 418, 486
TaiI ACGT 2 cut(s) 464, 699
TaqI TCGA 4 cut(s) 9, 134, 143, 282
TaqII GACCGA 1 cut(s) 68
TasI AATT 2 cut(s) 150, 366
TauI GCSGC 2 cut(s) 160, 212
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TscAI CASTG 1 cut(s) 529
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 2 cut(s) 246, 581
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 5 cut(s) 516, 591, 634, 641, 645
TspGWI ACGGA 2 cut(s) 208, 356
TspRI CASTG 1 cut(s) 529
VpaK11BI GGWCC 2 cut(s) 186, 558
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 3 cut(s) 321, 467, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.