MD10G1268600.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004106
Physical Location & Seq
Reverse (-)
36145832 .. 36149850
4019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1268600.v1.1.491

Sequence Viewer

Length: 789 bp
ATGACCAAAGAAGTTCCAGCGCACCCCCCGTCGGCAGTGCCGGAGCCAATGTGGGAGCAAGAAACTCCTCCGCAAACCCTAACAATGGTGGTCGAAGACGCACCCAAGACTTCAGCTACAGAAGATCAGGCCAAGACCGAGGCGAACGCTAAGCCGAGGGAGGATGACGACGAGCCCGAAGAAGGAGAGATCGTCGAGGACCAAGACTCGGAGTCGTCGAAGCCTTCGAAGGGAATTGCGCCGCAGTCTCACGCCCTGGAGCACTCATGGACCTTCTGGTTCGATAGCCCTGCCGCAAAGCCCGCCAAGTCCAAGCAGGAGGATTGGGGCAGCTCCATCCGCCCGATCTACACCTTCACTACCGTCGAGGAGTTTTGGAGCATTTACAACAATGTACGTCATCCGAGCAAGTTCACTGTGGGGACCGATTTCCATTGTTTCAAAAACAAAATTGAGCCAAAGTGGGAGGATCCTGTGTGTGCTAATGGAGGAAACTGGACTGTCACTAATCCTAAGGGGAAATCTGATACCCCTTGGTTGTATACGTTGCTAGCAATGATAGGAGAACAGTTTGATCATGGAGATGAAATCTGTGGAGCAGTAGTCAGTGTCAGAAAAAGCCAAGAAAAAATAACTCTTTGGACCAAGAATGCAACAAATGAAGCTGCTCAGGTGAGCATTGGGAAACAATGGAAGGGGTTTCTGGACTACAATGAAACTATTGGGTTCGTATTTCATGAGGATGCGATTAGACTTGGGAGAGGTGCCAAGAATAAATACATAGCCTGA

Protein Analysis

263

Amino Acids

29.45

Weight (kDa)

4.98

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 86 - 240 1.7e-54 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 764
AccI GTMKAC 1 cut(s) 542
AciI CCGC 5 cut(s) 71, 242, 294, 303, 340
AclWI GGATC 2 cut(s) 464, 477
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 5 cut(s) 31, 85, 182, 208, 230
AgsI TTSAA 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 211
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 3 cut(s) 116, 333, 665
AluI AGCT 3 cut(s) 116, 333, 665
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 2 cut(s) 464, 477
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 2 cut(s) 330, 665
AspLEI GCGC 2 cut(s) 22, 241
AspS9I GGNCC 4 cut(s) 199, 270, 423, 642
AsuHPI GGTGA 1 cut(s) 685
AsuII TTCGAA 1 cut(s) 227
AsuNHI GCTAGC 1 cut(s) 550
AvaII GGWCC 4 cut(s) 199, 270, 423, 642
AxyI CCTNAGG 1 cut(s) 513
BamHI GGATCC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 764
BanII GRGCYC 1 cut(s) 177
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 342, 652
BccI CCATC 1 cut(s) 344
BcgI CGANNNNNNTGC 2 cut(s) 272, 306
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 4 cut(s) 242, 294, 331, 666
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 4 cut(s) 243, 295, 332, 667
Bme1390I CCNGG 1 cut(s) 257
Bme18I GGWCC 4 cut(s) 199, 270, 423, 642
BmeRI GACNNNNNGTC 1 cut(s) 211
BmgT120I GGNCC 4 cut(s) 199, 270, 423, 642
BmiI GGNNCC 4 cut(s) 45, 424, 471, 766
BmrFI CCNGG 1 cut(s) 257
BmsI GCATC 1 cut(s) 733
BmtI GCTAGC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 1 cut(s) 278
Bpu10I CCTNAGC 1 cut(s) 669
Bpu1102I GCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 227
BsaJI CCNNGG 4 cut(s) 138, 155, 255, 533
BsaXI ACNNNNNCTCC 6 cut(s) 35, 65, 177, 207, 588, 618
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 85, 182, 208, 230
Bse1I ACTGG 1 cut(s) 500
Bse21I CCTNAGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 4 cut(s) 138, 155, 255, 533
BseGI GGATG 4 cut(s) 169, 336, 400, 748
BseLI CCNNNNNNNGG 5 cut(s) 31, 85, 182, 208, 230
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 1 cut(s) 683
BseNI ACTGG 1 cut(s) 500
BseRI GAGGAG 2 cut(s) 57, 383
BseXI GCAGC 2 cut(s) 342, 652
BshFI GGCC 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 264
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 5 cut(s) 31, 85, 182, 208, 230
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 2 cut(s) 177, 264
Bsp143I GATC 5 cut(s) 124, 189, 345, 469, 574
Bsp1720I GCTNAGC 1 cut(s) 150
BspACI CCGC 5 cut(s) 71, 242, 294, 303, 340
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 682
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 4 cut(s) 45, 424, 471, 766
BspOI GCTAGC 1 cut(s) 554
BspPI GGATC 2 cut(s) 464, 477
BspT104I TTCGAA 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 764
BsrDI GCAATG 1 cut(s) 561
BsrI ACTGG 1 cut(s) 500
BssECI CCNNGG 4 cut(s) 138, 155, 255, 533
BssMI GATC 5 cut(s) 124, 189, 345, 469, 574
BssNAI GTATAC 1 cut(s) 543
BssT1I CCWWGG 1 cut(s) 533
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 1 cut(s) 257
Bst4CI ACNGT 4 cut(s) 364, 418, 502, 570
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 303, 552
BstDEI CTNAG 3 cut(s) 150, 513, 669
BstF5I GGATG 4 cut(s) 169, 336, 400, 748
BstHHI GCGC 2 cut(s) 22, 241
BstKTI GATC 5 cut(s) 127, 192, 348, 472, 577
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 5 cut(s) 124, 189, 345, 469, 574
BstMWI GCNNNNNNNGC 2 cut(s) 302, 339
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 342, 652
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BstZ17I GTATAC 1 cut(s) 543
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 1 cut(s) 131
BtsCI GGATG 4 cut(s) 169, 336, 400, 748
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 3 cut(s) 42, 414, 613
Cac8I GCNNGC 2 cut(s) 303, 552
CciI TCATGA 1 cut(s) 736
CfoI GCGC 2 cut(s) 22, 241
Cfr13I GGNCC 4 cut(s) 199, 270, 423, 642
CseI GACGC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 395
CviAII CATG 3 cut(s) 267, 578, 737
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 3 cut(s) 150, 513, 669
DpnI GATC 5 cut(s) 126, 191, 347, 471, 576
DpnII GATC 5 cut(s) 124, 189, 345, 469, 574
DriI GACNNNNNGTC 1 cut(s) 211
Eam1105I GACNNNNNGTC 1 cut(s) 211
EciI GGCGGA 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 4 cut(s) 199, 270, 423, 642
Eco57I CTGAAG 1 cut(s) 96
Eco81I CCTNAGG 1 cut(s) 513
EcoRII CCWGG 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 3 cut(s) 270, 581, 740
FaiI YATR 5 cut(s) 268, 543, 579, 738, 782
FaqI GGGAC 1 cut(s) 436
FatI CATG 3 cut(s) 266, 577, 736
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 4 cut(s) 242, 294, 331, 666
FokI GGATG 4 cut(s) 176, 323, 387, 755
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 4 cut(s) 242, 294, 331, 666
FspBI CTAG 1 cut(s) 551
GlaI GCGC 2 cut(s) 21, 240
GluI GCNGC 4 cut(s) 242, 294, 331, 666
GsuI CTGGAG 1 cut(s) 278
HaeIII GGCC 1 cut(s) 131
HapII CCGG 1 cut(s) 41
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 22, 241
Hin1II CATG 3 cut(s) 270, 581, 740
Hin6I GCGC 2 cut(s) 20, 239
HinP1I GCGC 2 cut(s) 20, 239
HinfI GANTC 2 cut(s) 206, 212
HpaII CCGG 1 cut(s) 41
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 2 cut(s) 414, 543
Hpy188I TCNGA 4 cut(s) 211, 405, 526, 614
Hpy188III TCNNGA 2 cut(s) 704, 737
Hpy8I GTNNAC 2 cut(s) 414, 543
Hpy99I CGWCG 5 cut(s) 34, 173, 197, 220, 368
HpyAV CCTTC 6 cut(s) 176, 223, 234, 283, 364, 688
HpyCH4III ACNGT 4 cut(s) 364, 418, 502, 570
HpyCH4IV ACGT 2 cut(s) 397, 545
HpyCH4V TGCA 1 cut(s) 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 339
HpyF3I CTNAG 3 cut(s) 150, 513, 669
HpySE526I ACGT 2 cut(s) 397, 545
Hsp92II CATG 3 cut(s) 270, 581, 740
HspAI GCGC 2 cut(s) 20, 239
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 5 cut(s) 124, 189, 345, 469, 574
LmnI GCTCC 6 cut(s) 43, 55, 259, 338, 378, 596
Lsp1109I GCAGC 2 cut(s) 342, 652
LweI GCATC 1 cut(s) 733
MaeI CTAG 1 cut(s) 551
MaeII ACGT 2 cut(s) 397, 545
MaeIII GTNAC 1 cut(s) 502
MalI GATC 5 cut(s) 126, 191, 347, 471, 576
MboI GATC 5 cut(s) 124, 189, 345, 469, 574
MboII GAAGA 3 cut(s) 107, 134, 191
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 177, 264
MluCI AATT 2 cut(s) 234, 450
MlyI GAGTC 2 cut(s) 200, 221
MslI CAYNNNNRTG 2 cut(s) 582, 741
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 2 cut(s) 302, 339
NdeII GATC 5 cut(s) 124, 189, 345, 469, 574
NheI GCTAGC 1 cut(s) 550
NlaIII CATG 3 cut(s) 270, 581, 740
NlaIV GGNNCC 4 cut(s) 45, 424, 471, 766
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 1 cut(s) 502
NspV TTCGAA 1 cut(s) 227
PagI TCATGA 1 cut(s) 736
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 215, 224
PctI GAATGC 1 cut(s) 655
PkrI GCNGC 4 cut(s) 243, 295, 332, 667
PleI GAGTC 2 cut(s) 200, 220
PpsI GAGTC 2 cut(s) 200, 220
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 4 cut(s) 45, 424, 471, 766
PspPI GGNCC 4 cut(s) 199, 270, 423, 642
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
RseI CAYNNNNRTG 2 cut(s) 582, 741
SatI GCNGC 4 cut(s) 242, 294, 331, 666
Sau3AI GATC 5 cut(s) 124, 189, 345, 469, 574
Sau96I GGNCC 4 cut(s) 199, 270, 423, 642
SchI GAGTC 2 cut(s) 200, 221
ScrFI CCNGG 1 cut(s) 257
SduI GDGCHC 2 cut(s) 177, 264
SetI ASST 9 cut(s) 118, 275, 335, 356, 400, 548, 667, 675, 766
SfaNI GCATC 1 cut(s) 733
SfcI CTRYAG 1 cut(s) 117
SfuI TTCGAA 1 cut(s) 227
SinI GGWCC 4 cut(s) 199, 270, 423, 642
SmiMI CAYNNNNRTG 2 cut(s) 582, 741
Sse9I AATT 2 cut(s) 234, 450
SsiI CCGC 5 cut(s) 71, 242, 294, 303, 340
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 255
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 4 cut(s) 364, 418, 502, 570
TaiI ACGT 2 cut(s) 400, 548
TaqI TCGA 6 cut(s) 93, 195, 218, 227, 282, 366
TaqII GACCGA 2 cut(s) 152, 440
TasI AATT 2 cut(s) 234, 450
TauI GCSGC 2 cut(s) 244, 296
TscAI CASTG 3 cut(s) 42, 421, 613
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 330, 665
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 4 cut(s) 600, 675, 725, 729
TspRI CASTG 3 cut(s) 42, 421, 613
VpaK11BI GGWCC 4 cut(s) 199, 270, 423, 642
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.