MD05G1292800.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004106
Physical Location & Seq
Reverse (-)
42431624 .. 42434681
3058 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1292800.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGTGGTCGAAGACGCACCCAAGACTTCAGCTTCCGAAGATCAGGCCAAGACCGAGGCGAACCCTACACCGAGGGAGGACGACGACGAGCCCGAAGAAGGAGAGATCGTCGGCGACCAAGATTCGGCGTCTTCGAAGCCGTCGAAAGGAATAGCGCCGCAGTCACTCCCTCTGGAGCACTCCTGGACCTTCTGGTTCGATAGCCCCGCCGCCAAGTCCGCCAAGTCCAAGCAGGAGGATTGGGGCAGCTCCATCCGCCCGATCTACACCTTCTCTACCGTCGAGGAGTTCTGGAGCATTTACAACAATATACATCATCCGAGCAAGTTGAATGTGGGGACGGATTTCCATTGTTTCAAATACAAAATTGAGCCAAAGTGGGAGGATCCTGTTTGTGCTAATGGAGGAAAGTGGACTGTCACTTATCCTAAAGGGAAATCTGATACCTCTTGGTTGTATACGTTGCTAGCAATAATTGGAGAACAGTTTGATCATGGCGATGAAATCTGTGGAGCAGTTGTCAATGTCAGACCTAGGCAAGAAAAAATATCTCTTTGGACCAAGAATGCAGCAAATGAAGCTGCTCAGCTGAGCATTGGGAAACAATGGAAGGGGTTTCTGGATTACAATGAAACTATTGGATACATATTTCATGAGGATGCGATGAGACAGGAGAGAGGTGCCAAGAATAAATACGTAGCCTGA

Protein Analysis

235

Amino Acids

26.38

Weight (kDa)

5.05

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 57 - 211 3.6e-57 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 458
AciI CCGC 5 cut(s) 158, 207, 210, 219, 256
AclWI GGATC 2 cut(s) 380, 393
AcuI CTGAAG 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 128
AfiI CCNNNNNNNGG 3 cut(s) 98, 124, 146
AgsI TTSAA 2 cut(s) 331, 358
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 4 cut(s) 32, 249, 581, 589
AluI AGCT 4 cut(s) 32, 249, 581, 589
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 661
AlwI GGATC 2 cut(s) 380, 393
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 246, 569, 581
AspA2I CCTAGG 1 cut(s) 533
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 186, 558
AsuII TTCGAA 1 cut(s) 134
AsuNHI GCTAGC 1 cut(s) 466
AvaII GGWCC 2 cut(s) 186, 558
AvrII CCTAGG 1 cut(s) 533
BamHI GGATCC 1 cut(s) 385
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 18, 123
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 3 cut(s) 258, 568, 581
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 1 cut(s) 184
BciVI GTATCC 1 cut(s) 635
BclI TGATCA 1 cut(s) 490
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 2 cut(s) 467, 534
BfoI RGCGCY 1 cut(s) 158
BfuI GTATCC 1 cut(s) 635
BisI GCNGC 5 cut(s) 158, 210, 247, 570, 582
BlnI CCTAGG 1 cut(s) 533
BlpI GCTNAGC 2 cut(s) 585, 590
BlsI GCNGC 5 cut(s) 159, 211, 248, 571, 583
Bme1390I CCNGG 1 cut(s) 184
Bme18I GGWCC 2 cut(s) 186, 558
BmgT120I GGNCC 2 cut(s) 186, 558
BmiI GGNNCC 2 cut(s) 387, 682
BmrFI CCNGG 1 cut(s) 184
BmsI GCATC 1 cut(s) 649
BmtI GCTAGC 1 cut(s) 470
BpiI GAAGAC 2 cut(s) 18, 123
BpmI CTGGAG 2 cut(s) 194, 313
Bpu1102I GCTNAGC 2 cut(s) 585, 590
Bpu14I TTCGAA 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 697
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 3 cut(s) 54, 71, 533
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 124, 146
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 3 cut(s) 54, 71, 533
BseGI GGATG 3 cut(s) 252, 316, 664
BseLI CCNNNNNNNGG 3 cut(s) 98, 124, 146
BseMII CTCAG 2 cut(s) 581, 599
BseRI GAGGAG 1 cut(s) 299
BseXI GCAGC 3 cut(s) 258, 568, 581
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 680
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 3 cut(s) 98, 124, 146
BsmAI GTCTC 1 cut(s) 661
BsmFI GGGAC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 93, 180
Bsp143I GATC 5 cut(s) 40, 105, 261, 385, 490
Bsp1720I GCTNAGC 2 cut(s) 585, 590
BspACI CCGC 5 cut(s) 158, 207, 210, 219, 256
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 2 cut(s) 582, 598
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 387, 682
BspOI GCTAGC 1 cut(s) 470
BspPI GGATC 2 cut(s) 380, 393
BspT104I TTCGAA 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 680
BssECI CCNNGG 3 cut(s) 54, 71, 533
BssMI GATC 5 cut(s) 40, 105, 261, 385, 490
BssNAI GTATAC 1 cut(s) 459
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 533
Bst1107I GTATAC 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 184
Bst4CI ACNGT 3 cut(s) 280, 418, 486
BstACI GRCGYC 1 cut(s) 128
BstBAI YACGTR 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 468
BstDEI CTNAG 2 cut(s) 585, 590
BstF5I GGATG 3 cut(s) 252, 316, 664
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 5 cut(s) 43, 108, 264, 388, 493
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 5 cut(s) 40, 105, 261, 385, 490
BstMWI GCNNNNNNNGC 3 cut(s) 218, 255, 578
BstNI CCWGG 1 cut(s) 184
BstSCI CCNGG 1 cut(s) 182
BstSNI TACGTA 1 cut(s) 697
BstV1I GCAGC 3 cut(s) 258, 568, 581
BstV2I GAAGAC 2 cut(s) 18, 123
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BstZ17I GTATAC 1 cut(s) 459
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 1 cut(s) 47
BtgZI GCGATG 2 cut(s) 513, 677
BtsCI GGATG 3 cut(s) 252, 316, 664
Cac8I GCNNGC 1 cut(s) 468
CciI TCATGA 1 cut(s) 652
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 186, 558
CseI GACGC 2 cut(s) 23, 117
CviAII CATG 2 cut(s) 494, 653
DdeI CTNAG 2 cut(s) 585, 590
DpnI GATC 5 cut(s) 42, 107, 263, 387, 492
DpnII GATC 5 cut(s) 40, 105, 261, 385, 490
EciI GGCGGA 2 cut(s) 208, 245
Eco105I TACGTA 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 186, 558
Eco57I CTGAAG 1 cut(s) 12
EcoRII CCWGG 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 2 cut(s) 497, 656
FaiI YATR 5 cut(s) 311, 459, 495, 647, 654
FaqI GGGAC 1 cut(s) 352
FatI CATG 2 cut(s) 493, 652
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 490
FblI GTMKAC 1 cut(s) 458
Fnu4HI GCNGC 5 cut(s) 158, 210, 247, 570, 582
FokI GGATG 3 cut(s) 239, 303, 671
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 5 cut(s) 158, 210, 247, 570, 582
FspBI CTAG 2 cut(s) 467, 534
GlaI GCGC 1 cut(s) 156
GluI GCNGC 5 cut(s) 158, 210, 247, 570, 582
GsuI CTGGAG 2 cut(s) 194, 313
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 1 cut(s) 47
HgaI GACGC 2 cut(s) 23, 117
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 2 cut(s) 497, 656
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HinfI GANTC 1 cut(s) 122
Hpy166II GTNNAC 2 cut(s) 414, 459
Hpy188I TCNGA 4 cut(s) 37, 321, 442, 530
Hpy188III TCNNGA 4 cut(s) 173, 292, 620, 653
Hpy8I GTNNAC 2 cut(s) 414, 459
Hpy99I CGWCG 5 cut(s) 86, 89, 113, 145, 284
HpyAV CCTTC 4 cut(s) 92, 199, 280, 604
HpyCH4III ACNGT 3 cut(s) 280, 418, 486
HpyCH4IV ACGT 2 cut(s) 461, 696
HpyCH4V TGCA 1 cut(s) 569
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 255, 578
HpyF3I CTNAG 2 cut(s) 585, 590
HpySE526I ACGT 2 cut(s) 461, 696
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 2 cut(s) 497, 656
HspAI GCGC 1 cut(s) 155
Ksp22I TGATCA 1 cut(s) 490
Kzo9I GATC 5 cut(s) 40, 105, 261, 385, 490
LmnI GCTCC 4 cut(s) 175, 254, 294, 512
Lsp1109I GCAGC 3 cut(s) 258, 568, 581
LweI GCATC 1 cut(s) 649
MaeI CTAG 2 cut(s) 467, 534
MaeII ACGT 2 cut(s) 461, 696
MaeIII GTNAC 2 cut(s) 162, 418
MalI GATC 5 cut(s) 42, 107, 263, 387, 492
MboI GATC 5 cut(s) 40, 105, 261, 385, 490
MboII GAAGA 4 cut(s) 23, 50, 107, 123
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 93, 180
MluCI AATT 2 cut(s) 366, 474
MslI CAYNNNNRTG 2 cut(s) 498, 657
MspA1I CMGCKG 1 cut(s) 589
MspR9I CCNGG 1 cut(s) 184
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 1 cut(s) 184
MwoI GCNNNNNNNGC 3 cut(s) 218, 255, 578
NdeII GATC 5 cut(s) 40, 105, 261, 385, 490
NheI GCTAGC 1 cut(s) 466
NlaIII CATG 2 cut(s) 497, 656
NlaIV GGNNCC 2 cut(s) 387, 682
NmuCI GTSAC 2 cut(s) 162, 418
NspV TTCGAA 1 cut(s) 134
PagI TCATGA 1 cut(s) 652
PcsI WCGNNNNNNNCGW 3 cut(s) 90, 131, 140
PctI GAATGC 1 cut(s) 571
PfeI GAWTC 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 182
PkrI GCNGC 5 cut(s) 159, 211, 248, 571, 583
Ppu21I YACGTR 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 2 cut(s) 387, 682
PspPI GGNCC 2 cut(s) 186, 558
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 589
RseI CAYNNNNRTG 2 cut(s) 498, 657
SatI GCNGC 5 cut(s) 158, 210, 247, 570, 582
Sau3AI GATC 5 cut(s) 40, 105, 261, 385, 490
Sau96I GGNCC 2 cut(s) 186, 558
ScrFI CCNGG 1 cut(s) 184
SduI GDGCHC 2 cut(s) 93, 180
SfaNI GCATC 1 cut(s) 649
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 2 cut(s) 186, 558
SmiMI CAYNNNNRTG 2 cut(s) 498, 657
SnaBI TACGTA 1 cut(s) 697
Sse9I AATT 2 cut(s) 366, 474
SsiI CCGC 5 cut(s) 158, 207, 210, 219, 256
SspMI CTAG 2 cut(s) 467, 534
StyD4I CCNGG 1 cut(s) 182
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 3 cut(s) 280, 418, 486
TaiI ACGT 2 cut(s) 464, 699
TaqI TCGA 5 cut(s) 9, 134, 143, 198, 282
TaqII GACCGA 1 cut(s) 68
TasI AATT 2 cut(s) 366, 474
TauI GCSGC 2 cut(s) 160, 212
TfiI GAWTC 1 cut(s) 122
TseFI GTSAC 2 cut(s) 162, 418
TseI GCWGC 3 cut(s) 246, 569, 581
Tsp45I GTSAC 2 cut(s) 162, 418
TspDTI ATGAA 4 cut(s) 516, 591, 641, 645
TspGWI ACGGA 1 cut(s) 356
VpaK11BI GGWCC 2 cut(s) 186, 558
XmaJI CCTAGG 1 cut(s) 533
XmiI GTMKAC 1 cut(s) 458
XspI CTAG 2 cut(s) 467, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.