pycom10g22520

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0004106
Physical Location & Seq
Reverse (-)
24871248 .. 24873955
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22520.1

Sequence Viewer

Length: 810 bp
ATGACCAAAGAAGTTCCAGCGCACCCCGCTCTAGAGTGCCGGAGCCAATATGGGGTATTGGCTGCTGTTGACCCAATAATGTTATATTCCCCCCTTTCCAATTTCGTTTTACCCCTATGCGTTAGTTTTGTTCTGTTTCATATAAAAGAAAAGTTTTGCACACTAATTCTCAAAACAACCAATCTTCAACAACTTCCACGTCGCCCTAGCATCACCCTAACAAAAAAACGCAACTCTTTCACGTTTGGCACAAAAGAAACGTCGGGAAAGAAGAGACAGCAAGAAACTCCTCCGCAAACCCTAACGATGGTGGTCGAAGACGCACCCAAGACTTCAGCTTCAGAAGATCAGGCCAAGACCGAGGCGAACACTAAGCCGAGGGAGGATGACGACGAGCCCGAAGAAGGAGAGATCGTCGGCGACCAAGACTCGGCGTCGTCGAAGCCTTCGAAGGGAATTGCGCCGCAGTCTCACGCCCTGGAGCACTCCTGGACCTTCTGGTTCGATAGCCCTGCCGCAAAGCCCGCTAAGTCCAAGCAGGAGGATTGGGGCAGCTCCATCCGCCCGATCTACACCTTCACTACCGTCGAGGAGTTCTGGAGCATTTACAACAATGTACGTCATCCGAGCAAGCTCATTGTGGGGACGGATTTCCATTGTTTCAAATACAAAATTGAGCCAAAGTGGGAGGATCCTGTGTGTGCTAATGGAGGAAACTGGACTGTCCCTTATCCTAGGGGGAAATCTGATACCTTTTGGTTGTATACGTTGCTAGCAATGATAGGAGAACAGTTTGATCGATGGAGATGA

Protein Analysis

270

Amino Acids

30.61

Weight (kDa)

6.61

Isoelectric Point (pI)

53.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 160 - 267 8.4e-36 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 29
AccI GTMKAC 1 cut(s) 764
AciI CCGC 6 cut(s) 27, 293, 464, 516, 525, 562
AclWI GGATC 2 cut(s) 686, 699
AcuI CTGAAG 2 cut(s) 318, 324
AcyI GRCGYC 1 cut(s) 434
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 5 cut(s) 52, 307, 404, 430, 452
AgsI TTSAA 2 cut(s) 188, 664
AhdI GACNNNNNGTC 1 cut(s) 433
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 2 cut(s) 477, 488
AluBI AGCT 3 cut(s) 338, 555, 634
AluI AGCT 3 cut(s) 338, 555, 634
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 2 cut(s) 268, 474
AlwI GGATC 2 cut(s) 686, 699
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 2 cut(s) 62, 552
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AspA2I CCTAGG 1 cut(s) 734
AspLEI GCGC 2 cut(s) 22, 463
AspS9I GGNCC 1 cut(s) 492
AsuHPI GGTGA 1 cut(s) 205
AsuII TTCGAA 1 cut(s) 449
AsuNHI GCTAGC 1 cut(s) 772
AvaII GGWCC 1 cut(s) 492
AvrII CCTAGG 1 cut(s) 734
BamHI GGATCC 1 cut(s) 691
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 49, 564
BccI CCATC 3 cut(s) 301, 566, 795
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BciT130I CCWGG 2 cut(s) 479, 490
BcoDI GTCTC 2 cut(s) 268, 474
BfaI CTAG 4 cut(s) 32, 207, 735, 773
BisI GCNGC 4 cut(s) 63, 464, 516, 553
BlnI CCTAGG 1 cut(s) 734
BlsI GCNGC 4 cut(s) 64, 465, 517, 554
Bme1390I CCNGG 2 cut(s) 479, 490
Bme18I GGWCC 1 cut(s) 492
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgBI CACGTC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 2 cut(s) 44, 693
BmrFI CCNGG 2 cut(s) 479, 490
BmsI GCATC 1 cut(s) 219
BmtI GCTAGC 1 cut(s) 776
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 2 cut(s) 500, 619
Bpu14I TTCGAA 1 cut(s) 449
Bsa29I ATCGAT 1 cut(s) 799
BsaHI GRCGYC 1 cut(s) 434
BsaJI CCNNGG 4 cut(s) 360, 377, 477, 734
BsaXI ACNNNNNCTCC 2 cut(s) 399, 429
Bsc4I CCNNNNNNNGG 5 cut(s) 52, 307, 404, 430, 452
Bse1I ACTGG 1 cut(s) 722
Bse3DI GCAATG 1 cut(s) 783
BseBI CCWGG 2 cut(s) 479, 490
BseCI ATCGAT 1 cut(s) 799
BseDI CCNNGG 4 cut(s) 360, 377, 477, 734
BseGI GGATG 3 cut(s) 391, 558, 622
BseLI CCNNNNNNNGG 5 cut(s) 52, 307, 404, 430, 452
BseMI GCAATG 1 cut(s) 783
BseNI ACTGG 1 cut(s) 722
BseRI GAGGAG 2 cut(s) 279, 605
BseXI GCAGC 2 cut(s) 49, 564
BshFI GGCC 1 cut(s) 353
BshVI ATCGAT 1 cut(s) 799
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 2 cut(s) 658, 710
BslI CCNNNNNNNGG 5 cut(s) 52, 307, 404, 430, 452
BsmAI GTCTC 2 cut(s) 268, 474
BsmFI GGGAC 2 cut(s) 658, 710
BsnI GGCC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 399, 486
Bsp143I GATC 5 cut(s) 346, 411, 567, 691, 796
BspACI CCGC 6 cut(s) 27, 293, 464, 516, 525, 562
BspANI GGCC 1 cut(s) 353
BspDI ATCGAT 1 cut(s) 799
BspLI GGNNCC 2 cut(s) 44, 693
BspOI GCTAGC 1 cut(s) 776
BspPI GGATC 2 cut(s) 686, 699
BspT104I TTCGAA 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 1 cut(s) 722
BssECI CCNNGG 4 cut(s) 360, 377, 477, 734
BssMI GATC 5 cut(s) 346, 411, 567, 691, 796
BssNAI GTATAC 1 cut(s) 765
BssNI GRCGYC 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 734
Bst1107I GTATAC 1 cut(s) 765
Bst2UI CCWGG 2 cut(s) 479, 490
Bst4CI ACNGT 3 cut(s) 586, 724, 792
Bst6I CTCTTC 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 434
BstBI TTCGAA 1 cut(s) 449
BstC8I GCNNGC 3 cut(s) 525, 632, 774
BstDEI CTNAG 2 cut(s) 372, 528
BstF5I GGATG 3 cut(s) 391, 558, 622
BstHHI GCGC 2 cut(s) 22, 463
BstKTI GATC 5 cut(s) 349, 414, 570, 694, 799
BstMAI GTCTC 2 cut(s) 268, 474
BstMBI GATC 5 cut(s) 346, 411, 567, 691, 796
BstMWI GCNNNNNNNGC 3 cut(s) 26, 524, 561
BstNI CCWGG 2 cut(s) 479, 490
BstSCI CCNGG 2 cut(s) 477, 488
BstV1I GCAGC 2 cut(s) 49, 564
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 691
BstYI RGATCY 1 cut(s) 691
BstZ17I GTATAC 1 cut(s) 765
Bsu15I ATCGAT 1 cut(s) 799
BsuRI GGCC 1 cut(s) 353
BsuTUI ATCGAT 1 cut(s) 799
BtrI CACGTC 1 cut(s) 200
BtsCI GGATG 3 cut(s) 391, 558, 622
Cac8I GCNNGC 3 cut(s) 525, 632, 774
CfoI GCGC 2 cut(s) 22, 463
Cfr13I GGNCC 1 cut(s) 492
ClaI ATCGAT 1 cut(s) 799
CseI GACGC 2 cut(s) 329, 423
Csp6I GTAC 1 cut(s) 617
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 2 cut(s) 372, 528
DpnI GATC 5 cut(s) 348, 413, 569, 693, 798
DpnII GATC 5 cut(s) 346, 411, 567, 691, 796
DriI GACNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 266
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 266
EciI GGCGGA 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 734
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 492
Eco57I CTGAAG 2 cut(s) 318, 324
EcoRII CCWGG 2 cut(s) 477, 488
EcoT14I CCWWGG 1 cut(s) 734
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 734
FaiI YATR 6 cut(s) 51, 85, 118, 141, 143, 765
FaqI GGGAC 2 cut(s) 658, 710
FauI CCCGC 2 cut(s) 34, 532
FblI GTMKAC 1 cut(s) 764
Fnu4HI GCNGC 4 cut(s) 63, 464, 516, 553
FokI GGATG 3 cut(s) 398, 545, 609
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 4 cut(s) 63, 464, 516, 553
FspBI CTAG 4 cut(s) 32, 207, 735, 773
GlaI GCGC 2 cut(s) 21, 462
GluI GCNGC 4 cut(s) 63, 464, 516, 553
GsuI CTGGAG 2 cut(s) 500, 619
HaeIII GGCC 1 cut(s) 353
HapII CCGG 1 cut(s) 40
HgaI GACGC 2 cut(s) 329, 423
HhaI GCGC 2 cut(s) 22, 463
Hin1I GRCGYC 1 cut(s) 434
Hin6I GCGC 2 cut(s) 20, 461
HinP1I GCGC 2 cut(s) 20, 461
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 428
HpaII CCGG 1 cut(s) 40
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 70, 765
Hpy188I TCNGA 3 cut(s) 343, 627, 748
Hpy188III TCNNGA 3 cut(s) 32, 264, 598
Hpy8I GTNNAC 2 cut(s) 70, 765
Hpy99I CGWCG 7 cut(s) 204, 265, 395, 419, 439, 442, 590
HpyAV CCTTC 5 cut(s) 398, 445, 456, 505, 586
HpyCH4III ACNGT 3 cut(s) 586, 724, 792
HpyCH4IV ACGT 5 cut(s) 199, 242, 260, 619, 767
HpyCH4V TGCA 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 524, 561
HpyF3I CTNAG 2 cut(s) 372, 528
HpySE526I ACGT 5 cut(s) 199, 242, 260, 619, 767
Hsp92I GRCGYC 1 cut(s) 434
HspAI GCGC 2 cut(s) 20, 461
Kzo9I GATC 5 cut(s) 346, 411, 567, 691, 796
LmnI GCTCC 4 cut(s) 42, 481, 560, 600
Lsp1109I GCAGC 2 cut(s) 49, 564
LweI GCATC 1 cut(s) 219
MaeI CTAG 4 cut(s) 32, 207, 735, 773
MaeII ACGT 5 cut(s) 199, 242, 260, 619, 767
MalI GATC 5 cut(s) 348, 413, 569, 693, 798
MbiI CCGCTC 1 cut(s) 29
MboI GATC 5 cut(s) 346, 411, 567, 691, 796
MboII GAAGA 5 cut(s) 176, 283, 329, 356, 413
MflI RGATCY 1 cut(s) 691
MhlI GDGCHC 2 cut(s) 399, 486
MluCI AATT 4 cut(s) 100, 165, 456, 672
MlyI GAGTC 1 cut(s) 422
MnlI CCTC 8 cut(s) 300, 355, 372, 376, 535, 583, 682, 704
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 2 cut(s) 479, 490
MvaI CCWGG 2 cut(s) 479, 490
MwoI GCNNNNNNNGC 3 cut(s) 26, 524, 561
NdeII GATC 5 cut(s) 346, 411, 567, 691, 796
NheI GCTAGC 1 cut(s) 772
NlaIV GGNNCC 2 cut(s) 44, 693
NmeAIII GCCGAG 2 cut(s) 402, 410
NspV TTCGAA 1 cut(s) 449
PcsI WCGNNNNNNNCGW 3 cut(s) 396, 437, 446
PfoI TCCNGGA 1 cut(s) 488
PkrI GCNGC 4 cut(s) 64, 465, 517, 554
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 477, 488
PspGI CCWGG 2 cut(s) 477, 488
PspN4I GGNNCC 2 cut(s) 44, 693
PspPI GGNCC 1 cut(s) 492
PsuI RGATCY 1 cut(s) 691
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
SatI GCNGC 4 cut(s) 63, 464, 516, 553
Sau3AI GATC 5 cut(s) 346, 411, 567, 691, 796
Sau96I GGNCC 1 cut(s) 492
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 2 cut(s) 479, 490
SduI GDGCHC 2 cut(s) 399, 486
SfaNI GCATC 1 cut(s) 219
SfuI TTCGAA 1 cut(s) 449
SinI GGWCC 1 cut(s) 492
Sse9I AATT 4 cut(s) 100, 165, 456, 672
SsiI CCGC 6 cut(s) 27, 293, 464, 516, 525, 562
SspMI CTAG 4 cut(s) 32, 207, 735, 773
StyD4I CCNGG 2 cut(s) 477, 488
StyI CCWWGG 1 cut(s) 734
TaaI ACNGT 3 cut(s) 586, 724, 792
TaiI ACGT 5 cut(s) 202, 245, 263, 622, 770
TaqI TCGA 6 cut(s) 315, 440, 449, 504, 588, 799
TaqII GACCGA 1 cut(s) 374
TasI AATT 4 cut(s) 100, 165, 456, 672
TauI GCSGC 2 cut(s) 466, 518
TseI GCWGC 2 cut(s) 62, 552
TspDTI ATGAA 1 cut(s) 128
TspGWI ACGGA 1 cut(s) 662
VpaK11BI GGWCC 1 cut(s) 492
XbaI TCTAGA 1 cut(s) 31
XmaJI CCTAGG 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 764
XspI CTAG 4 cut(s) 32, 207, 735, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.