RLG00000031954

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004106
Physical Location & Seq
Forward (+)
9796334 .. 9798548
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031954

Sequence Viewer

Length: 699 bp
ATGGCGGTGGAAGACGCAGGCAAGACTTCAGCTTCGGAAGATCAGAGCAAGACGGAGTCGAACCCTAAATCAATGCAAGACGAGAACGAGCTCGAGGAAGGAGAGATCGTCGGCGGCGGCGAGGAGGAGGAGTCTTCGAAGATCGCAAAGCCGCTGCGCCACCCTTTGGAGCACTCATGGACCTTCTGGTTCGATAGCCCCTCGGCCAAGAACTCCAAGCCCAAGCAAGAGGATTGGGGCAGCTCTATTCGCCCGATCTACACCTTCTCTACTGTTGAGGAATTCTGGGGTATATACAATAATATACGACATCCAAGCAAGTTGTCTGTGGGGACAGATCTCCATTGTTTCAAAAACAAAATTGAGCCTAAGTGGGAGGATCCTGTTTGTGCTAACGGAGGGAAATGGACTCTAACTTTTACTAGAGGGAAATCTGATAATTCCTGGCTGCATACGTTGCTAGCACTGATTGGGGAACAGTTTGATCATGGAGATGAAATATGTGGAGCAGTTGTCAATGTCAGAAATAGGCAAGACAAAATTTCTATTTGGACCAAGAATGCAGCATATGAGGCAGCTCAGGTGAGCATTGGAAAACAATGGAAGGGTTTTCTGGATAGCAACGAAACTATAGGCTTCATATTTCATGAAGATGCAAGGCGACAGGAGAGAAGTGCCAAGAATAAATACACAGTGTGA

Protein Analysis

233

Amino Acids

26.31

Weight (kDa)

5.46

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 55 - 209 1.3e-57 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 166
AciI CCGC 4 cut(s) 5, 114, 117, 152
AclWI GGATC 2 cut(s) 374, 387
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 2 cut(s) 281, 540
AcuI CTGAAG 1 cut(s) 12
AdeI CACNNNGTG 1 cut(s) 696
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 1 cut(s) 352
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 4 cut(s) 32, 91, 243, 578
AluI AGCT 4 cut(s) 32, 91, 243, 578
Alw21I GWGCWC 2 cut(s) 93, 174
AlwI GGATC 2 cut(s) 374, 387
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 5 cut(s) 154, 240, 448, 563, 575
ApoI RAATTY 2 cut(s) 281, 540
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 2 cut(s) 180, 552
AsuHPI GGTGA 1 cut(s) 595
AsuII TTCGAA 1 cut(s) 137
AsuNHI GCTAGC 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 2 cut(s) 180, 552
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 18, 126
Bbv12I GWGCWC 2 cut(s) 93, 174
BbvI GCAGC 5 cut(s) 141, 252, 435, 575, 587
BciT130I CCWGG 1 cut(s) 445
BclI TGATCA 1 cut(s) 484
BfaI CTAG 2 cut(s) 423, 461
BfmI CTRYAG 1 cut(s) 630
BglII AGATCT 1 cut(s) 337
BisI GCNGC 8 cut(s) 115, 118, 152, 155, 241, 449, 564, 576
BlsI GCNGC 8 cut(s) 116, 119, 153, 156, 242, 450, 565, 577
Bme1390I CCNGG 1 cut(s) 445
Bme18I GGWCC 2 cut(s) 180, 552
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 2 cut(s) 180, 552
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 1 cut(s) 445
BmsI GCATC 1 cut(s) 643
BmtI GCTAGC 1 cut(s) 464
BpiI GAAGAC 2 cut(s) 18, 126
Bpu10I CCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 201
BsaXI ACNNNNNCTCC 6 cut(s) 93, 116, 123, 146, 498, 528
Bsc4I CCNNNNNNNGG 1 cut(s) 166
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 593
BseRI GAGGAG 3 cut(s) 137, 140, 143
BseXI GCAGC 5 cut(s) 141, 252, 435, 575, 587
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 2 cut(s) 93, 174
BsiHKCI CYCGRG 1 cut(s) 92
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 346
BsmI GAATGC 1 cut(s) 565
BsnI GGCC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 93, 174
Bsp143I GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
BspACI CCGC 4 cut(s) 5, 114, 117, 152
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 592
BspHI TCATGA 1 cut(s) 646
BspLI GGNNCC 1 cut(s) 381
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 2 cut(s) 374, 387
BspT104I TTCGAA 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 3 cut(s) 274, 480, 694
BstAPI GCANNNNNTGC 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 19, 462
BstDEI CTNAG 2 cut(s) 369, 579
BstF5I GGATG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 7 cut(s) 43, 108, 144, 258, 340, 382, 487
BstMBI GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
BstMWI GCNNNNNNNGC 3 cut(s) 249, 457, 572
BstNI CCWGG 1 cut(s) 445
BstSCI CCNGG 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 630
BstV1I GCAGC 5 cut(s) 141, 252, 435, 575, 587
BstV2I GAAGAC 2 cut(s) 18, 126
BstX2I RGATCY 2 cut(s) 337, 379
BstYI RGATCY 2 cut(s) 337, 379
BsuRI GGCC 1 cut(s) 206
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 2 cut(s) 464, 699
Cac8I GCNNGC 2 cut(s) 19, 462
CciI TCATGA 1 cut(s) 646
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 2 cut(s) 180, 552
CseI GACGC 1 cut(s) 23
CviAII CATG 3 cut(s) 177, 488, 647
DdeI CTNAG 2 cut(s) 369, 579
DpnI GATC 7 cut(s) 42, 107, 143, 257, 339, 381, 486
DpnII GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
DraIII CACNNNGTG 1 cut(s) 696
EaeI YGGCCR 1 cut(s) 204
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 180, 552
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 3 cut(s) 180, 491, 650
FaqI GGGAC 1 cut(s) 346
FatI CATG 3 cut(s) 176, 487, 646
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 8 cut(s) 115, 118, 152, 155, 241, 449, 564, 576
FokI GGATG 1 cut(s) 297
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 8 cut(s) 115, 118, 152, 155, 241, 449, 564, 576
FspBI CTAG 2 cut(s) 423, 461
GlaI GCGC 1 cut(s) 158
GluI GCNGC 8 cut(s) 115, 118, 152, 155, 241, 449, 564, 576
HaeIII GGCC 1 cut(s) 206
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 159
Hin1II CATG 3 cut(s) 180, 491, 650
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HinfI GANTC 3 cut(s) 56, 131, 409
HphI GGTGA 1 cut(s) 595
Hpy188I TCNGA 4 cut(s) 37, 45, 436, 524
Hpy188III TCNNGA 2 cut(s) 614, 647
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 4 cut(s) 92, 193, 274, 598
HpyCH4III ACNGT 3 cut(s) 274, 480, 694
HpyCH4IV ACGT 1 cut(s) 455
HpyCH4V TGCA 4 cut(s) 76, 451, 563, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 249, 457, 572
HpyF3I CTNAG 2 cut(s) 369, 579
HpySE526I ACGT 1 cut(s) 455
Hsp92II CATG 3 cut(s) 180, 491, 650
HspAI GCGC 1 cut(s) 157
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
LmnI GCTCC 2 cut(s) 169, 506
LpnPI CCDG 9 cut(s) 3, 172, 271, 396, 430, 457, 566, 599, 650
Lsp1109I GCAGC 5 cut(s) 141, 252, 435, 575, 587
LweI GCATC 1 cut(s) 643
MaeI CTAG 2 cut(s) 423, 461
MaeII ACGT 1 cut(s) 455
MalI GATC 7 cut(s) 42, 107, 143, 257, 339, 381, 486
MboI GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
MboII GAAGA 5 cut(s) 23, 50, 126, 151, 662
MflI RGATCY 2 cut(s) 337, 379
MhlI GDGCHC 2 cut(s) 93, 174
MluCI AATT 4 cut(s) 281, 360, 439, 540
MlyI GAGTC 3 cut(s) 65, 140, 403
MslI CAYNNNNRTG 2 cut(s) 492, 651
MspA1I CMGCKG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 445
Mva1269I GAATGC 1 cut(s) 565
MvaI CCWGG 1 cut(s) 445
MwoI GCNNNNNNNGC 3 cut(s) 249, 457, 572
NdeI CATATG 1 cut(s) 568
NdeII GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 3 cut(s) 180, 491, 650
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 182
NspV TTCGAA 1 cut(s) 137
PaeR7I CTCGAG 1 cut(s) 92
PagI TCATGA 1 cut(s) 646
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 1 cut(s) 565
PflFI GACNNNGTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 8 cut(s) 116, 119, 153, 156, 242, 450, 565, 577
PleI GAGTC 3 cut(s) 64, 139, 403
PpsI GAGTC 3 cut(s) 64, 139, 403
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 2 cut(s) 180, 552
PspXI VCTCGAGB 1 cut(s) 92
PsuI RGATCY 2 cut(s) 337, 379
PsyI GACNNNGTC 1 cut(s) 55
RseI CAYNNNNRTG 2 cut(s) 492, 651
SacI GAGCTC 1 cut(s) 93
SatI GCNGC 8 cut(s) 115, 118, 152, 155, 241, 449, 564, 576
Sau3AI GATC 7 cut(s) 40, 105, 141, 255, 337, 379, 484
Sau96I GGNCC 2 cut(s) 180, 552
SchI GAGTC 3 cut(s) 65, 140, 403
ScrFI CCNGG 1 cut(s) 445
SduI GDGCHC 2 cut(s) 93, 174
SetI ASST 8 cut(s) 34, 93, 185, 245, 266, 458, 580, 585
SfaNI GCATC 1 cut(s) 643
SfcI CTRYAG 1 cut(s) 630
Sfr274I CTCGAG 1 cut(s) 92
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 2 cut(s) 180, 552
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 492, 651
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 4 cut(s) 281, 360, 439, 540
SsiI CCGC 4 cut(s) 5, 114, 117, 152
SspMI CTAG 2 cut(s) 423, 461
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 274, 480, 694
TaiI ACGT 1 cut(s) 458
TaqI TCGA 4 cut(s) 59, 93, 137, 192
TasI AATT 4 cut(s) 281, 360, 439, 540
TauI GCSGC 3 cut(s) 117, 120, 154
TscAI CASTG 2 cut(s) 471, 699
TseI GCWGC 5 cut(s) 154, 240, 448, 563, 575
TspDTI ATGAA 4 cut(s) 510, 628, 635, 663
TspGWI ACGGA 2 cut(s) 68, 411
TspRI CASTG 2 cut(s) 471, 699
Tth111I GACNNNGTC 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 166
VpaK11BI GGWCC 2 cut(s) 180, 552
XapI RAATTY 2 cut(s) 281, 540
XhoI CTCGAG 1 cut(s) 92
XspI CTAG 2 cut(s) 423, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.