RchiOBHm_Chr5g0012781

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004106
Physical Location & Seq
Forward (+)
8678338 .. 8680987
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29333

Sequence Viewer

Length: 696 bp
ATGGCGGTGGAAGACGCAGGCTCTTCAGCTTCGGAAGATCAGAGCAAGACGGAGTCGAACCCTAAATCAATGCAAGACGAGAACGAGCTGGAAGAAGGAGAGATCGTCAGCGGCGGCGGCGACGAGGTGTCTTCGAAGATCGCAAAGCCGCTGCACCACCCTCTGGAGCACTCATGGACCTTCTGGTTCGATAGCCCCTCGGCCAAGAACTCCAAGCCCAAGCAAGAGGATTGGGGCAGCTCTATTCGCCCGATCTACACCTTCTCTACTGTTGAGGAATTCTGGGGTATATACAATAATATACGCCATCCAAGCAAGTTGTCTATGGGGACGGACCTCCATTGTTTCAAAAACAAAATTGAGCCTAAGTGGGAGGATCCTGTTTGTGCTAATGGAGGGAAATGGACTCTAACTTTTACTAAAGGGAAATCTGATCATCCCTGGCTGCATACGTTGCTAGCACTGATTGGGGAACAGTTTGATCATGGAGATGAAATATGTGGAGCAGTTGTCAATGTCAGAAGTAGGCAAGAAAAAATTGCTATTTGGACCAAGAATGCAGCAAATGAGACTGCTCAGTTGAGCATTGGAAAACAATGGAAGGGCTTTCTGGATAACAACGAAGCTATAGGCTTCATATTTCATGACGATGCAAGGCGACAGGAAAGAGGTGCCAAGAATAGATACACAGTGTGA

Protein Analysis

231

Amino Acids

26.05

Weight (kDa)

5.61

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 54 - 207 1.8e-57 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 163
AciI CCGC 5 cut(s) 5, 111, 114, 117, 149
AclWI GGATC 2 cut(s) 371, 384
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 9
AdeI CACNNNGTG 1 cut(s) 693
AfiI CCNNNNNNNGG 1 cut(s) 163
AgsI TTSAA 1 cut(s) 349
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 440
AluBI AGCT 4 cut(s) 29, 88, 240, 626
AluI AGCT 4 cut(s) 29, 88, 240, 626
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 2 cut(s) 371, 384
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 4 cut(s) 151, 237, 445, 560
ApoI RAATTY 1 cut(s) 278
AspS9I GGNCC 3 cut(s) 177, 334, 549
AsuII TTCGAA 1 cut(s) 134
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 3 cut(s) 177, 334, 549
BamHI GGATCC 1 cut(s) 376
BanI GGYRCC 1 cut(s) 671
BbsI GAAGAC 2 cut(s) 18, 123
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 4 cut(s) 138, 249, 432, 572
BccI CCATC 1 cut(s) 315
BciT130I CCWGG 1 cut(s) 442
BclI TGATCA 2 cut(s) 433, 481
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 627
BisI GCNGC 8 cut(s) 112, 115, 118, 149, 152, 238, 446, 561
BlsI GCNGC 8 cut(s) 113, 116, 119, 150, 153, 239, 447, 562
Bme1390I CCNGG 1 cut(s) 442
Bme18I GGWCC 3 cut(s) 177, 334, 549
BmeRI GACNNNNNGTC 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 177, 334, 549
BmiI GGNNCC 2 cut(s) 378, 673
BmrFI CCNGG 1 cut(s) 442
BmsI GCATC 1 cut(s) 640
BmtI GCTAGC 1 cut(s) 461
BpiI GAAGAC 2 cut(s) 18, 123
BpmI CTGGAG 1 cut(s) 185
Bpu14I TTCGAA 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 198, 440
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 495, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseBI CCWGG 1 cut(s) 442
BseDI CCNNGG 2 cut(s) 198, 440
BseGI GGATG 2 cut(s) 307, 436
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 1 cut(s) 590
BseXI GCAGC 4 cut(s) 138, 249, 432, 572
BsgI GTGCAG 1 cut(s) 137
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 671
BsiHKAI GWGCWC 1 cut(s) 171
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 163
BsmAI GTCTC 1 cut(s) 563
BsmFI GGGAC 1 cut(s) 343
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 1 cut(s) 203
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
BspACI CCGC 5 cut(s) 5, 111, 114, 117, 149
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 1 cut(s) 589
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 2 cut(s) 378, 673
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 2 cut(s) 371, 384
BspQI GCTCTTC 1 cut(s) 28
BspT104I TTCGAA 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 671
BssECI CCNNGG 2 cut(s) 198, 440
BssMI GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 3 cut(s) 271, 477, 691
Bst6I CTCTTC 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 454
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 19, 459
BstDEI CTNAG 2 cut(s) 366, 576
BstF5I GGATG 2 cut(s) 307, 436
BstKTI GATC 7 cut(s) 40, 105, 141, 255, 379, 436, 484
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
BstMWI GCNNNNNNNGC 4 cut(s) 117, 246, 312, 454
BstNI CCWGG 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 440
BstSFI CTRYAG 1 cut(s) 627
BstV1I GCAGC 4 cut(s) 138, 249, 432, 572
BstV2I GAAGAC 2 cut(s) 18, 123
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 2 cut(s) 307, 436
BtsIMutI CAGTG 2 cut(s) 461, 696
Cac8I GCNNGC 2 cut(s) 19, 459
CciI TCATGA 1 cut(s) 643
Cfr13I GGNCC 3 cut(s) 177, 334, 549
CseI GACGC 1 cut(s) 23
CviAII CATG 3 cut(s) 174, 485, 644
DdeI CTNAG 2 cut(s) 366, 576
DpnI GATC 7 cut(s) 39, 104, 140, 254, 378, 435, 483
DpnII GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
DraIII CACNNNGTG 1 cut(s) 693
DriI GACNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 201
Eam1104I CTCTTC 1 cut(s) 28
Eam1105I GACNNNNNGTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 28
Eco47I GGWCC 3 cut(s) 177, 334, 549
Eco57I CTGAAG 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 440
FaeI CATG 3 cut(s) 177, 488, 647
FaqI GGGAC 1 cut(s) 343
FatI CATG 3 cut(s) 173, 484, 643
FbaI TGATCA 2 cut(s) 433, 481
Fnu4HI GCNGC 8 cut(s) 112, 115, 118, 149, 152, 238, 446, 561
FokI GGATG 2 cut(s) 294, 423
Fsp4HI GCNGC 8 cut(s) 112, 115, 118, 149, 152, 238, 446, 561
FspBI CTAG 1 cut(s) 458
GluI GCNGC 8 cut(s) 112, 115, 118, 149, 152, 238, 446, 561
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 1 cut(s) 203
HgaI GACGC 1 cut(s) 23
Hin1II CATG 3 cut(s) 177, 488, 647
HinfI GANTC 2 cut(s) 53, 406
Hpy188I TCNGA 4 cut(s) 34, 42, 433, 521
Hpy188III TCNNGA 3 cut(s) 164, 611, 644
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 89, 190, 271, 595
HpyCH4III ACNGT 3 cut(s) 271, 477, 691
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 5 cut(s) 73, 154, 448, 560, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 246, 312, 454
HpyF3I CTNAG 2 cut(s) 366, 576
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 3 cut(s) 177, 488, 647
Ksp22I TGATCA 2 cut(s) 433, 481
Kzo9I GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
LguI GCTCTTC 1 cut(s) 28
LmnI GCTCC 2 cut(s) 166, 503
Lsp1109I GCAGC 4 cut(s) 138, 249, 432, 572
LweI GCATC 1 cut(s) 640
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 452
MalI GATC 7 cut(s) 39, 104, 140, 254, 378, 435, 483
MboI GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
MboII GAAGA 6 cut(s) 15, 23, 47, 104, 123, 148
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 3 cut(s) 278, 357, 537
MlyI GAGTC 2 cut(s) 62, 400
MnlI CCTC 9 cut(s) 118, 171, 208, 220, 268, 347, 367, 389, 662
MslI CAYNNNNRTG 2 cut(s) 489, 648
MspA1I CMGCKG 2 cut(s) 111, 151
MspR9I CCNGG 1 cut(s) 442
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 1 cut(s) 442
MwoI GCNNNNNNNGC 4 cut(s) 117, 246, 312, 454
NdeII GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 3 cut(s) 177, 488, 647
NlaIV GGNNCC 2 cut(s) 378, 673
NmeAIII GCCGAG 1 cut(s) 179
NspV TTCGAA 1 cut(s) 134
PagI TCATGA 1 cut(s) 643
PciSI GCTCTTC 1 cut(s) 28
PctI GAATGC 1 cut(s) 562
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 8 cut(s) 113, 116, 119, 150, 153, 239, 447, 562
PleI GAGTC 2 cut(s) 61, 400
PpsI GAGTC 2 cut(s) 61, 400
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 2 cut(s) 378, 673
PspPI GGNCC 3 cut(s) 177, 334, 549
PsuI RGATCY 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 52
RseI CAYNNNNRTG 2 cut(s) 489, 648
SapI GCTCTTC 1 cut(s) 28
SatI GCNGC 8 cut(s) 112, 115, 118, 149, 152, 238, 446, 561
Sau3AI GATC 7 cut(s) 37, 102, 138, 252, 376, 433, 481
Sau96I GGNCC 3 cut(s) 177, 334, 549
SchI GAGTC 2 cut(s) 62, 400
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 3 cut(s) 177, 334, 549
SmiMI CAYNNNNRTG 2 cut(s) 489, 648
Sse9I AATT 3 cut(s) 278, 357, 537
SsiI CCGC 5 cut(s) 5, 111, 114, 117, 149
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 1 cut(s) 440
TaaI ACNGT 3 cut(s) 271, 477, 691
TaiI ACGT 1 cut(s) 455
TaqI TCGA 3 cut(s) 56, 134, 189
TasI AATT 3 cut(s) 278, 357, 537
TauI GCSGC 4 cut(s) 114, 117, 120, 151
TscAI CASTG 2 cut(s) 468, 696
TseI GCWGC 4 cut(s) 151, 237, 445, 560
TspDTI ATGAA 3 cut(s) 507, 625, 632
TspGWI ACGGA 2 cut(s) 65, 347
TspRI CASTG 2 cut(s) 468, 696
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 3 cut(s) 177, 334, 549
XapI RAATTY 1 cut(s) 278
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.