RLG00000031941

Belongs to the eukaryotic initiation factor 4E family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004106
Physical Location & Seq
Forward (+)
9689280 .. 9689720
441 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031941

Sequence Viewer

Length: 348 bp
ATGGCGGTGGAAGACGCAGGCAAGACTTCAGCTTCGGAAGATCAGAGCAAGACGGAGTCGAACCCTAAATCAATGCAAGACGAGAACGAGCTCGAGGAAGGAGAGATCGTCGGCGGCGGCGAGGAGGTGTCTTCGAAGATCGCAAAGCCGCTGCGCCACCCTTTGGAGCACTCATGGACCTTCTGGTTCGATAGCCCCTCGGCCAAGAACTCCAAGCCCAAGCAAGAGGATTGGGGCAGCTCTATTCGCCTGATCTACACCTTGTCTACTGTTGAGGAATTCTGGGGGGTTTGGTTTTTCATGAGTGGGAATTTTGGGAAAGATAAGAACATGAATCTTGGAAAGTAG

Protein Analysis

116

Amino Acids

12.93

Weight (kDa)

4.82

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IF4E PF01652 54 - 101 1.6e-09 Eukaryotic initiation factor 4E
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 163
AccI GTMKAC 1 cut(s) 266
AciI CCGC 4 cut(s) 5, 114, 117, 149
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 2 cut(s) 278, 310
AcuI CTGAAG 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 163
AluBI AGCT 3 cut(s) 32, 91, 240
AluI AGCT 3 cut(s) 32, 91, 240
Alw21I GWGCWC 2 cut(s) 93, 171
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 151, 237
ApoI RAATTY 2 cut(s) 278, 310
ArsI GACNNNNNNTTYG 2 cut(s) 16, 48
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 177
AsuII TTCGAA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 1 cut(s) 177
BanII GRGCYC 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 18, 123
Bbv12I GWGCWC 2 cut(s) 93, 171
BbvI GCAGC 2 cut(s) 138, 249
BisI GCNGC 5 cut(s) 115, 118, 149, 152, 238
BlsI GCNGC 5 cut(s) 116, 119, 150, 153, 239
Bme18I GGWCC 1 cut(s) 177
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 177
BpiI GAAGAC 2 cut(s) 18, 123
Bpu14I TTCGAA 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bsc4I CCNNNNNNNGG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 198
BseLI CCNNNNNNNGG 1 cut(s) 163
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 2 cut(s) 138, 249
BshFI GGCC 1 cut(s) 203
BsiHKAI GWGCWC 2 cut(s) 93, 171
BsiHKCI CYCGRG 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 163
BsnI GGCC 1 cut(s) 203
BsoBI CYCGRG 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 93, 171
Bsp143I GATC 4 cut(s) 40, 105, 138, 252
BspACI CCGC 4 cut(s) 5, 114, 117, 149
BspANI GGCC 1 cut(s) 203
BspHI TCATGA 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 4 cut(s) 40, 105, 138, 252
Bst4CI ACNGT 1 cut(s) 271
BstBI TTCGAA 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 19
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 4 cut(s) 43, 108, 141, 255
BstMBI GATC 4 cut(s) 40, 105, 138, 252
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstV1I GCAGC 2 cut(s) 138, 249
BstV2I GAAGAC 2 cut(s) 18, 123
BsuRI GGCC 1 cut(s) 203
Cac8I GCNNGC 1 cut(s) 19
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 177
CseI GACGC 1 cut(s) 23
CviAII CATG 3 cut(s) 174, 301, 331
CviJI RGCY 7 cut(s) 32, 91, 148, 195, 203, 217, 240
CviKI_1 RGCY 7 cut(s) 32, 91, 148, 195, 203, 217, 240
DpnI GATC 4 cut(s) 42, 107, 140, 254
DpnII GATC 4 cut(s) 40, 105, 138, 252
EaeI YGGCCR 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 91
Eco57I CTGAAG 1 cut(s) 12
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 91
EcoRI GAATTC 1 cut(s) 278
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 3 cut(s) 177, 304, 334
FaiI YATR 3 cut(s) 175, 302, 332
FatI CATG 3 cut(s) 173, 300, 330
FblI GTMKAC 1 cut(s) 266
Fnu4HI GCNGC 5 cut(s) 115, 118, 149, 152, 238
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 5 cut(s) 115, 118, 149, 152, 238
GlaI GCGC 1 cut(s) 155
GluI GCNGC 5 cut(s) 115, 118, 149, 152, 238
HaeIII GGCC 1 cut(s) 203
HgaI GACGC 1 cut(s) 23
HhaI GCGC 1 cut(s) 156
Hin1II CATG 3 cut(s) 177, 304, 334
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 2 cut(s) 56, 334
Hpy166II GTNNAC 1 cut(s) 267
Hpy188I TCNGA 2 cut(s) 37, 45
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 1 cut(s) 267
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 92, 190
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
Hsp92II CATG 3 cut(s) 177, 304, 334
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 4 cut(s) 40, 105, 138, 252
LmnI GCTCC 1 cut(s) 166
LpnPI CCDG 4 cut(s) 3, 169, 263, 268
Lsp1109I GCAGC 2 cut(s) 138, 249
MalI GATC 4 cut(s) 42, 107, 140, 254
MboI GATC 4 cut(s) 40, 105, 138, 252
MboII GAAGA 4 cut(s) 23, 50, 123, 148
MhlI GDGCHC 2 cut(s) 93, 171
MluCI AATT 2 cut(s) 278, 310
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 6 cut(s) 88, 115, 118, 208, 220, 268
MspA1I CMGCKG 1 cut(s) 151
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 4 cut(s) 40, 105, 138, 252
NlaIII CATG 3 cut(s) 177, 304, 334
NmeAIII GCCGAG 1 cut(s) 179
NspV TTCGAA 1 cut(s) 134
PaeR7I CTCGAG 1 cut(s) 92
PagI TCATGA 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PfeI GAWTC 1 cut(s) 334
PflFI GACNNNGTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 5 cut(s) 116, 119, 150, 153, 239
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 93
PspPI GGNCC 1 cut(s) 177
PspXI VCTCGAGB 1 cut(s) 92
PsyI GACNNNGTC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 93
SatI GCNGC 5 cut(s) 115, 118, 149, 152, 238
Sau3AI GATC 4 cut(s) 40, 105, 138, 252
Sau96I GGNCC 1 cut(s) 177
SchI GAGTC 1 cut(s) 65
SduI GDGCHC 2 cut(s) 93, 171
SetI ASST 6 cut(s) 34, 93, 129, 182, 242, 263
Sfr274I CTCGAG 1 cut(s) 92
SfuI TTCGAA 1 cut(s) 134
SinI GGWCC 1 cut(s) 177
SlaI CTCGAG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 2 cut(s) 278, 310
SsiI CCGC 4 cut(s) 5, 114, 117, 149
SstI GAGCTC 1 cut(s) 93
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 4 cut(s) 59, 93, 134, 189
TasI AATT 2 cut(s) 278, 310
TauI GCSGC 3 cut(s) 117, 120, 151
TfiI GAWTC 1 cut(s) 334
TseI GCWGC 2 cut(s) 151, 237
TspDTI ATGAA 2 cut(s) 289, 347
TspGWI ACGGA 1 cut(s) 68
Tth111I GACNNNGTC 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 177
XapI RAATTY 2 cut(s) 278, 310
XhoI CTCGAG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.