FvH4_7g09180

At1g28695-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002782
Physical Location & Seq
Forward (+)
8921438 .. 8925983
4546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09180.t1

Sequence Viewer

Length: 1146 bp
ATGGAACCTCTTCATCACCAGAAACATCACTCTTCTCAGCTCAGATCCAATCTCACATTCATCTCTTTACTCCTTGTTTGTGTTGTTTATCTCTCTATATGGTCTTCTTCCTCCTCCATCAACCCGCTCTTCGCCATCGAGGAGCACAATGAGTGCGTATCACAAAACCAGACGAACATGTTCGTCGAGCCTCAAGACGAGCTCGAGGCCACGTTATCCAGGGCTTCAATGGAGAACAAGACGGTGATCATTGCCGTGGTAAACAAGGCTTACGCAGTGCAAGATGTGAGAGCAGACACAACCATGCTCGACCTCTTTATCGAGAGCTTTTGGCAAGGGGAGGGCACGAGGGAGCTGATCGACCACCTGGTGGTGGTGGCGGTTGATCAGACGGCCTACGACCGGTGCCGGTTTCTGAGGCTGAACTGCTACAAATTGGAAACCGACGGTGTGGATTTCGGGGGAGAGAAGATTTACATGTCCCAGGATTTCATTCAGATGATGTGGAAGAGAACTTGGTTCTTGTTGGAGGTTCTCAAACGTGGTTACAGCTTCGTGTTCACGGACACTGATGTGGTGTGGCTAAGGAACCCATTTACAAAGTTAAGTAACAACGAAACTGAGGATTTACAAATAAGCACCGACATGTTCTTTGGAGATCCATGGAACACAACACAGTTAATCAATACCGGCTTCTACTACATCAAATCCAACAACAAGACCATCGCACTGTTTGATAAATGGTACAACTTGAAGGACAATGCCACTGGACTGAAAGAACAAGATGTATTACTAAACCTCCTTCGAGGTGGTATTGTTCCAGAATTGGGGCTTAGAGTTAGATTCTTGGATACTATATATTTCAGTGGTTTTTGCCAGAATAGCAGGGATGTTAGGGAAGTTAGTACCGTCCATGCCAATTGTTGTCGGAGTATTGTTGCGAAAGTGCAAGACTTGAAGGCTGTTCTCCGGGATTGGAAGAAGTTCAAGAAAGTAGTATCGCATAAAAGAGTTGATCTTAATGTGACTGCCAAAACTTTTCAATGGACCGGTCATTGGGGATGTTGGAACTCATGGAAGGTTCCGGCTAGCAACGGGAAACGGAAGCTGCCGAATAACACCGGCAAAGCTACTCATAAGGCATAA

Protein Analysis

382

Amino Acids

44.04

Weight (kDa)

8.31

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 119 - 319 3.1e-61 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 405
AccB7I CCANNNNNTGG 1 cut(s) 370
AccBSI CCGCTC 1 cut(s) 127
AciI CCGC 2 cut(s) 125, 380
AclWI GGATC 2 cut(s) 39, 653
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 2 cut(s) 746, 907
AfiI CCNNNNNNNGG 5 cut(s) 370, 373, 402, 827, 1056
AflIII ACRYGT 3 cut(s) 177, 477, 645
AgeI ACCGGT 2 cut(s) 402, 1049
AgsI TTSAA 5 cut(s) 228, 754, 958, 988, 1043
AjnI CCWGG 3 cut(s) 218, 366, 483
AjuI GAANNNNNNNTTGG 2 cut(s) 41, 73
AleI CACNNNNGTG 1 cut(s) 572
AluBI AGCT 7 cut(s) 40, 202, 327, 355, 552, 1108, 1130
AluI AGCT 7 cut(s) 40, 202, 327, 355, 552, 1108, 1130
Alw21I GWGCWC 2 cut(s) 147, 204
AlwI GGATC 2 cut(s) 39, 653
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 2 cut(s) 207, 393
ApeKI GCWGC 1 cut(s) 1108
ArsI GACNNNNNNTTYG 2 cut(s) 635, 667
AsiGI ACCGGT 2 cut(s) 402, 1049
Asp700I GAANNNNTTC 3 cut(s) 9, 179, 983
AspS9I GGNCC 1 cut(s) 1047
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 2 cut(s) 8, 256
AsuNHI GCTAGC 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 1047
BaeGI GKGCMC 1 cut(s) 347
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 204
BauI CACGAG 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 147, 204
BbvI GCAGC 1 cut(s) 1095
BccI CCATC 3 cut(s) 125, 143, 731
BceAI ACGGC 2 cut(s) 239, 408
BciT130I CCWGG 3 cut(s) 220, 368, 485
BciVI GTATCC 1 cut(s) 844
BclI TGATCA 2 cut(s) 246, 385
BcnI CCSGG 1 cut(s) 971
BfaI CTAG 1 cut(s) 1089
BfuI GTATCC 1 cut(s) 844
BisI GCNGC 1 cut(s) 1109
BlsI GCNGC 1 cut(s) 1110
Bme1390I CCNGG 4 cut(s) 220, 368, 485, 971
Bme18I GGWCC 1 cut(s) 1047
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 1047
BmiI GGNNCC 4 cut(s) 6, 407, 590, 1083
BmrFI CCNGG 4 cut(s) 220, 368, 485, 971
BmtI GCTAGC 1 cut(s) 1092
BpiI GAAGAC 1 cut(s) 96
Bpu10I CCTNAGC 1 cut(s) 584
BpuEI CTTGAG 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 971
BsaJI CCNNGG 4 cut(s) 219, 255, 483, 662
BsaWI WCCGGW 2 cut(s) 402, 1049
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 5 cut(s) 370, 373, 402, 827, 1056
Bse118I RCCGGY 5 cut(s) 402, 408, 689, 1049, 1121
Bse1I ACTGG 1 cut(s) 772
Bse3DI GCAATG 1 cut(s) 249
BseBI CCWGG 3 cut(s) 220, 368, 485
BseDI CCNNGG 4 cut(s) 219, 255, 483, 662
BseGI GGATG 2 cut(s) 895, 1067
BseLI CCNNNNNNNGG 5 cut(s) 370, 373, 402, 827, 1056
BseMI GCAATG 1 cut(s) 249
BseMII CTCAG 4 cut(s) 50, 55, 407, 612
BseNI ACTGG 1 cut(s) 772
BseRI GAGGAG 2 cut(s) 103, 155
BseSI GKGCMC 1 cut(s) 347
BseXI GCAGC 1 cut(s) 1095
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 2 cut(s) 209, 395
BshNI GGYRCC 1 cut(s) 405
BshTI ACCGGT 2 cut(s) 402, 1049
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 147, 204
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 7 cut(s) 403, 409, 690, 970, 1050, 1085, 1122
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 5 cut(s) 370, 373, 402, 827, 1056
BsmFI GGGAC 1 cut(s) 466
BsnI GGCC 2 cut(s) 209, 395
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 3 cut(s) 147, 204, 347
Bsp143I GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
Bsp19I CCATGG 1 cut(s) 662
BspACI CCGC 2 cut(s) 125, 380
BspANI GGCC 2 cut(s) 209, 395
BspCNI CTCAG 4 cut(s) 49, 54, 408, 613
BspLI GGNNCC 4 cut(s) 6, 407, 590, 1083
BspOI GCTAGC 1 cut(s) 1092
BspPI GGATC 2 cut(s) 39, 653
BspQI GCTCTTC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 405
BsrBI CCGCTC 1 cut(s) 127
BsrDI GCAATG 1 cut(s) 249
BsrFI RCCGGY 5 cut(s) 402, 408, 689, 1049, 1121
BsrI ACTGG 1 cut(s) 772
BssAI RCCGGY 5 cut(s) 402, 408, 689, 1049, 1121
BssECI CCNNGG 4 cut(s) 219, 255, 483, 662
BssMI GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
BssSI CACGAG 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 662
Bst2BI CACGAG 1 cut(s) 346
Bst2UI CCWGG 3 cut(s) 220, 368, 485
Bst4CI ACNGT 5 cut(s) 244, 449, 678, 732, 910
Bst6I CTCTTC 4 cut(s) 15, 37, 134, 503
BstC8I GCNNGC 1 cut(s) 1090
BstDEI CTNAG 6 cut(s) 36, 41, 416, 584, 621, 833
BstDSI CCRYGG 2 cut(s) 255, 662
BstF5I GGATG 2 cut(s) 895, 1067
BstKTI GATC 6 cut(s) 47, 249, 360, 388, 661, 1018
BstMBI GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 1 cut(s) 882
BstNI CCWGG 3 cut(s) 220, 368, 485
BstNSI RCATGY 3 cut(s) 181, 481, 649
BstSCI CCNGG 4 cut(s) 218, 366, 483, 969
BstSLI GKGCMC 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 1095
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 2 cut(s) 44, 658
BstYI RGATCY 2 cut(s) 44, 658
BsuI GTATCC 1 cut(s) 844
BsuRI GGCC 2 cut(s) 209, 395
BtgI CCRYGG 2 cut(s) 255, 662
BtgZI GCGATG 1 cut(s) 709
BtsCI GGATG 2 cut(s) 895, 1067
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 5 cut(s) 282, 567, 728, 765, 871
Cac8I GCNNGC 1 cut(s) 1090
Cfr10I RCCGGY 5 cut(s) 402, 408, 689, 1049, 1121
Cfr13I GGNCC 1 cut(s) 1047
CsiI ACCWGGT 1 cut(s) 366
Csp6I GTAC 2 cut(s) 745, 906
CspAI ACCGGT 2 cut(s) 402, 1049
CviAII CATG 7 cut(s) 178, 304, 478, 646, 663, 914, 1074
CviQI GTAC 2 cut(s) 745, 906
DdeI CTNAG 6 cut(s) 36, 41, 416, 584, 621, 833
DpnI GATC 6 cut(s) 46, 248, 359, 387, 660, 1017
DpnII GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
DraIII CACNNNGTG 1 cut(s) 370
Eam1104I CTCTTC 4 cut(s) 15, 37, 134, 503
EarI CTCTTC 4 cut(s) 15, 37, 134, 503
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 1047
Eco53kI GAGCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 3 cut(s) 218, 366, 483
EcoT14I CCWWGG 1 cut(s) 662
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 662
FaeI CATG 7 cut(s) 181, 307, 481, 649, 666, 917, 1077
FaqI GGGAC 1 cut(s) 466
FatI CATG 7 cut(s) 177, 303, 477, 645, 662, 913, 1073
FauI CCCGC 1 cut(s) 132
FbaI TGATCA 2 cut(s) 246, 385
Fnu4HI GCNGC 1 cut(s) 1109
FokI GGATG 2 cut(s) 902, 1074
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 1109
FspBI CTAG 1 cut(s) 1089
GluI GCNGC 1 cut(s) 1109
HaeIII GGCC 2 cut(s) 209, 395
HapII CCGG 7 cut(s) 403, 409, 690, 970, 1050, 1085, 1122
Hin1II CATG 7 cut(s) 181, 307, 481, 649, 666, 917, 1077
HinfI GANTC 1 cut(s) 843
HpaII CCGG 7 cut(s) 403, 409, 690, 970, 1050, 1085, 1122
HphI GGTGA 2 cut(s) 8, 256
Hpy166II GTNNAC 2 cut(s) 262, 561
Hpy188I TCNGA 5 cut(s) 44, 390, 417, 498, 930
Hpy188III TCNNGA 4 cut(s) 194, 322, 821, 988
Hpy8I GTNNAC 2 cut(s) 262, 561
Hpy99I CGWCG 2 cut(s) 188, 449
HpyAV CCTTC 4 cut(s) 748, 812, 952, 1072
HpyCH4III ACNGT 5 cut(s) 244, 449, 678, 732, 910
HpyCH4IV ACGT 2 cut(s) 212, 541
HpyCH4V TGCA 2 cut(s) 280, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 882
HpyF3I CTNAG 6 cut(s) 36, 41, 416, 584, 621, 833
HpySE526I ACGT 2 cut(s) 212, 541
Hsp92II CATG 7 cut(s) 181, 307, 481, 649, 666, 917, 1077
Ksp22I TGATCA 2 cut(s) 246, 385
Kzo9I GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 2 cut(s) 142, 352
Lsp1109I GCAGC 1 cut(s) 1095
MabI ACCWGGT 1 cut(s) 366
MaeI CTAG 1 cut(s) 1089
MaeII ACGT 2 cut(s) 212, 541
MaeIII GTNAC 3 cut(s) 545, 608, 1024
MalI GATC 6 cut(s) 46, 248, 359, 387, 660, 1017
MbiI CCGCTC 1 cut(s) 127
MboI GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
MboII GAAGA 7 cut(s) 24, 96, 99, 121, 481, 520, 991
MfeI CAATTG 1 cut(s) 919
MflI RGATCY 2 cut(s) 44, 658
MhlI GDGCHC 3 cut(s) 147, 204, 347
MluCI AATT 3 cut(s) 434, 824, 919
MmeI TCCRAC 4 cut(s) 507, 735, 908, 1046
MroXI GAANNNNTTC 3 cut(s) 9, 179, 983
MseI TTAA 3 cut(s) 605, 680, 1020
MslI CAYNNNNRTG 5 cut(s) 254, 302, 497, 572, 644
MspI CCGG 7 cut(s) 403, 409, 690, 970, 1050, 1085, 1122
MspR9I CCNGG 4 cut(s) 220, 368, 485, 971
MunI CAATTG 1 cut(s) 919
MvaI CCWGG 3 cut(s) 220, 368, 485
MwoI GCNNNNNNNGC 1 cut(s) 882
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 662
NdeII GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
NheI GCTAGC 1 cut(s) 1088
NlaIII CATG 7 cut(s) 181, 307, 481, 649, 666, 917, 1077
NlaIV GGNNCC 4 cut(s) 6, 407, 590, 1083
NmuCI GTSAC 1 cut(s) 1024
NspI RCATGY 3 cut(s) 181, 481, 649
OliI CACNNNNGTG 1 cut(s) 572
PaeR7I CTCGAG 1 cut(s) 203
PciI ACATGT 3 cut(s) 177, 477, 645
PciSI GCTCTTC 1 cut(s) 134
PdmI GAANNNNTTC 3 cut(s) 9, 179, 983
PfeI GAWTC 1 cut(s) 843
PflMI CCANNNNNTGG 1 cut(s) 370
PfoI TCCNGGA 1 cut(s) 969
PinAI ACCGGT 2 cut(s) 402, 1049
PkrI GCNGC 1 cut(s) 1110
PscI ACATGT 3 cut(s) 177, 477, 645
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 3 cut(s) 218, 366, 483
PspGI CCWGG 3 cut(s) 218, 366, 483
PspN4I GGNNCC 4 cut(s) 6, 407, 590, 1083
PspPI GGNCC 1 cut(s) 1047
PspXI VCTCGAGB 1 cut(s) 203
PsuI RGATCY 2 cut(s) 44, 658
RsaI GTAC 2 cut(s) 746, 907
RsaNI GTAC 2 cut(s) 745, 906
RseI CAYNNNNRTG 5 cut(s) 254, 302, 497, 572, 644
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 605, 680, 1020
SatI GCNGC 1 cut(s) 1109
Sau3AI GATC 6 cut(s) 44, 246, 357, 385, 658, 1015
Sau96I GGNCC 1 cut(s) 1047
ScrFI CCNGG 4 cut(s) 220, 368, 485, 971
SduI GDGCHC 3 cut(s) 147, 204, 347
SexAI ACCWGGT 1 cut(s) 366
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 1047
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 5 cut(s) 254, 302, 497, 572, 644
SmlI CTYRAG 2 cut(s) 192, 203
SmoI CTYRAG 2 cut(s) 192, 203
Sse9I AATT 3 cut(s) 434, 824, 919
SsiI CCGC 2 cut(s) 125, 380
SspMI CTAG 1 cut(s) 1089
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 4 cut(s) 218, 366, 483, 969
StyI CCWWGG 1 cut(s) 662
TaaI ACNGT 5 cut(s) 244, 449, 678, 732, 910
TaiI ACGT 2 cut(s) 215, 544
TaqI TCGA 7 cut(s) 138, 186, 204, 309, 321, 360, 805
TasI AATT 3 cut(s) 434, 824, 919
TfiI GAWTC 1 cut(s) 843
Tru1I TTAA 3 cut(s) 605, 680, 1020
Tru9I TTAA 3 cut(s) 605, 680, 1020
TscAI CASTG 5 cut(s) 282, 574, 735, 772, 871
TseFI GTSAC 1 cut(s) 1024
TseI GCWGC 1 cut(s) 1108
Tsp45I GTSAC 1 cut(s) 1024
TspDTI ATGAA 2 cut(s) 49, 481
TspGWI ACGGA 2 cut(s) 578, 1117
TspRI CASTG 5 cut(s) 282, 574, 735, 772, 871
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 1 cut(s) 1047
XceI RCATGY 3 cut(s) 181, 481, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 203
XmnI GAANNNNTTC 3 cut(s) 9, 179, 983
XspI CTAG 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.