RLG00000028846

At1g28695-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002782
Physical Location & Seq
Reverse (-)
29167549 .. 29170487
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028846

Sequence Viewer

Length: 1164 bp
ATGGAACCTTCTCATCACCAGAAACATCACTCTTCTTTGGGATCCAATCTTGCATTTCTCTCTCTACTCCTCGCTTGTGTTGTTTATCTCTCTATATGGTCTTCTTCCTCCTCTATCAACCCGCTTTTCGCCTTCCAGAAACTCGATGAGGGCGCATCACAAAGCCTGGCAAGTCATTTCTTGTTATTGAACTCCCGACTTATCCTAATAACAACGATCACATTCGACGAGCCTAGAGACGAGCTCGAGTCCACTTTATCTAAGGCTTCAATGGAGAACAAGACGGTGATCATTGCCGTGATAAACCAGGCTTACGCAGTGCAAGATGTGAATGCAGACACAACCATGCTCGACCTCTTCCTTGAGAGCTTTTGGCAAGGGGAGGGCACGAGGGAGCTGCTCGACCACCTGGTTCTGGTGGCGGTTGATCAAACGGCCTACGACCGGTGCCGGTTTCTGAGGCTGAATTGCTACAGATTGGAAACCGACGGCGTAGATTTCGGGGGAGAGAAGATTTTCATGTCCCAGGATTTCATCAAAATGATGTGGAAGAGAACTTGGTTCGTGTTGGAGGTTCTCAAACGTGGTTACAGCTTCGTATTCACGGACACTGATGTGATGTGGCTAAGGAACCCATTCACAAAGCTAAGTAAAAATGAAACCGAGGATTTACAAATAAGCACGGACATGTTCTTTGGAGATCCATGGAACACAACACAGTTAATCAATACTGGATTCTACTACATCAAATCCAACAACAAGACCATTGCACTGTTTGATAAATGGTACACCTTGAAGGACAATGCCACCGGAATTAAAGAACAGGATGTATTACTAAACCTAATCCGAGGTGGTATTGTTCCAGAGTTAGGTCTTAGAGTCAGGTTCTTGGATACTGTATATTTCAGTGGTTTTTGCCAGAATAGCAGGGATATTAGGGAAGTCAGTACTGTCCATTCCAATTGTTGTCGGAGTATTGTTGCGAAAGTACAAGATTTGAAGGCTGTTCTCCGAGATTGGAAGAAGTTCAAGAAAGTAGTGTCACATACGAGGAGGGTTAATCTTAATCTGACGGCCGAGAGATTCCAGTGGACTGGCCATTGGGGATGTTGGAACTCATGGAAGGTTCCGGCAAACACCGCCAAAGCAACTCATAAGTCCTAA

Protein Analysis

388

Amino Acids

44.58

Weight (kDa)

8.09

Isoelectric Point (pI)

28.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 133 - 333 1.1e-59 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 447
AciI CCGC 3 cut(s) 122, 422, 1140
AclWI GGATC 3 cut(s) 36, 49, 695
AcoI YGGCCR 2 cut(s) 1074, 1096
AfaI GTAC 3 cut(s) 788, 949, 990
AfiI CCNNNNNNNGG 3 cut(s) 415, 444, 869
AflIII ACRYGT 1 cut(s) 687
AgeI ACCGGT 1 cut(s) 444
AgsI TTSAA 5 cut(s) 190, 270, 796, 1000, 1030
AjnI CCWGG 4 cut(s) 165, 306, 408, 525
AleI CACNNNNGTG 1 cut(s) 614
AluBI AGCT 5 cut(s) 244, 369, 397, 594, 646
AluI AGCT 5 cut(s) 244, 369, 397, 594, 646
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 3 cut(s) 36, 49, 695
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 3 cut(s) 435, 1074, 1096
ApeKI GCWGC 1 cut(s) 397
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AsiGI ACCGGT 1 cut(s) 444
Asp700I GAANNNNTTC 3 cut(s) 515, 635, 1025
AspLEI GCGC 1 cut(s) 155
AsuHPI GGTGA 2 cut(s) 8, 298
AvaI CYCGRG 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 389
BalI TGGCCA 1 cut(s) 1098
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 447
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 384
BceAI ACGGC 4 cut(s) 281, 450, 505, 1089
BcgI CGANNNNNNTGC 2 cut(s) 379, 413
BciT130I CCWGG 4 cut(s) 167, 308, 410, 527
BciVI GTATCC 1 cut(s) 886
BclI TGATCA 2 cut(s) 288, 427
BcoDI GTCTC 1 cut(s) 231
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 472
BfuI GTATCC 1 cut(s) 886
BisI GCNGC 1 cut(s) 398
BlsI GCNGC 1 cut(s) 399
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 4 cut(s) 167, 308, 410, 527
BmeT110I CYCGRG 1 cut(s) 245
BmiI GGNNCC 5 cut(s) 6, 43, 449, 632, 1128
BmrFI CCNGG 4 cut(s) 167, 308, 410, 527
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 228, 260
Bpu10I CCTNAGC 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 383
BsaJI CCNNGG 4 cut(s) 525, 663, 704, 847
BsaWI WCCGGW 2 cut(s) 444, 809
Bsc4I CCNNNNNNNGG 3 cut(s) 415, 444, 869
Bse118I RCCGGY 2 cut(s) 444, 450
Bse1I ACTGG 3 cut(s) 736, 1087, 1099
Bse3DI GCAATG 2 cut(s) 291, 765
BseBI CCWGG 4 cut(s) 167, 308, 410, 527
BseDI CCNNGG 4 cut(s) 525, 663, 704, 847
BseGI GGATG 2 cut(s) 832, 1112
BseLI CCNNNNNNNGG 3 cut(s) 415, 444, 869
BseMI GCAATG 2 cut(s) 291, 765
BseMII CTCAG 1 cut(s) 449
BseNI ACTGG 3 cut(s) 736, 1087, 1099
BseRI GAGGAG 3 cut(s) 59, 100, 1066
BseSI GKGCMC 1 cut(s) 389
BseX3I CGGCCG 1 cut(s) 1074
BseXI GCAGC 1 cut(s) 384
Bsh1285I CGRYCG 2 cut(s) 445, 1077
BshFI GGCC 3 cut(s) 437, 1076, 1098
BshNI GGYRCC 1 cut(s) 447
BshTI ACCGGT 1 cut(s) 444
BsiEI CGRYCG 2 cut(s) 445, 1077
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 4 cut(s) 445, 451, 810, 1130
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 3 cut(s) 415, 444, 869
BsmAI GTCTC 1 cut(s) 231
BsmBI CGTCTC 1 cut(s) 231
BsmFI GGGAC 1 cut(s) 508
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 3 cut(s) 437, 1076, 1098
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 246, 389
Bsp143I GATC 5 cut(s) 41, 216, 288, 427, 700
Bsp19I CCATGG 1 cut(s) 704
BspACI CCGC 3 cut(s) 122, 422, 1140
BspANI GGCC 3 cut(s) 437, 1076, 1098
BspCNI CTCAG 1 cut(s) 450
BspLI GGNNCC 5 cut(s) 6, 43, 449, 632, 1128
BspPI GGATC 3 cut(s) 36, 49, 695
BspT107I GGYRCC 1 cut(s) 447
BsrDI GCAATG 2 cut(s) 291, 765
BsrFI RCCGGY 2 cut(s) 444, 450
BsrI ACTGG 3 cut(s) 736, 1087, 1099
BssAI RCCGGY 2 cut(s) 444, 450
BssECI CCNNGG 4 cut(s) 525, 663, 704, 847
BssMI GATC 5 cut(s) 41, 216, 288, 427, 700
BssSI CACGAG 1 cut(s) 388
BssT1I CCWWGG 1 cut(s) 704
Bst2BI CACGAG 1 cut(s) 388
Bst2UI CCWGG 4 cut(s) 167, 308, 410, 527
Bst4CI ACNGT 5 cut(s) 286, 720, 774, 898, 952
Bst6I CTCTTC 3 cut(s) 37, 362, 545
BstDEI CTNAG 5 cut(s) 261, 458, 626, 647, 875
BstDSI CCRYGG 1 cut(s) 704
BstF5I GGATG 2 cut(s) 832, 1112
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 5 cut(s) 44, 219, 291, 430, 703
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 5 cut(s) 41, 216, 288, 427, 700
BstMCI CGRYCG 2 cut(s) 445, 1077
BstMWI GCNNNNNNNGC 2 cut(s) 924, 1139
BstNI CCWGG 4 cut(s) 167, 308, 410, 527
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 4 cut(s) 165, 306, 408, 525
BstSFI CTRYAG 1 cut(s) 472
BstSLI GKGCMC 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 384
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 41, 700
BstXI CCANNNNNNTGG 1 cut(s) 1094
BstYI RGATCY 2 cut(s) 41, 700
BstZI CGGCCG 1 cut(s) 1074
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 3 cut(s) 437, 1076, 1098
BtgI CCRYGG 1 cut(s) 704
BtsCI GGATG 2 cut(s) 832, 1112
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 5 cut(s) 324, 609, 770, 913, 1094
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 2 cut(s) 444, 450
CsiI ACCWGGT 1 cut(s) 408
Csp6I GTAC 3 cut(s) 787, 948, 989
CspAI ACCGGT 1 cut(s) 444
CviAII CATG 5 cut(s) 346, 520, 688, 705, 1119
CviQI GTAC 3 cut(s) 787, 948, 989
DdeI CTNAG 5 cut(s) 261, 458, 626, 647, 875
DpnI GATC 5 cut(s) 43, 218, 290, 429, 702
DpnII GATC 5 cut(s) 41, 216, 288, 427, 700
EaeI YGGCCR 2 cut(s) 1074, 1096
EagI CGGCCG 1 cut(s) 1074
Eam1104I CTCTTC 3 cut(s) 37, 362, 545
EarI CTCTTC 3 cut(s) 37, 362, 545
Ecl136II GAGCTC 1 cut(s) 244
EclXI CGGCCG 1 cut(s) 1074
Eco130I CCWWGG 1 cut(s) 704
Eco24I GRGCYC 1 cut(s) 246
Eco52I CGGCCG 1 cut(s) 1074
Eco53kI GAGCTC 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 244
EcoRII CCWGG 4 cut(s) 165, 306, 408, 525
EcoT14I CCWWGG 1 cut(s) 704
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 704
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 5 cut(s) 349, 523, 691, 708, 1122
FaqI GGGAC 1 cut(s) 508
FatI CATG 5 cut(s) 345, 519, 687, 704, 1118
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 2 cut(s) 288, 427
Fnu4HI GCNGC 1 cut(s) 398
FokI GGATG 2 cut(s) 839, 1119
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 398
FspBI CTAG 1 cut(s) 234
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 398
HaeIII GGCC 3 cut(s) 437, 1076, 1098
HapII CCGG 4 cut(s) 445, 451, 810, 1130
HhaI GCGC 1 cut(s) 155
Hin1II CATG 5 cut(s) 349, 523, 691, 708, 1122
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 4 cut(s) 248, 735, 879, 1083
HpaII CCGG 4 cut(s) 445, 451, 810, 1130
HphI GGTGA 2 cut(s) 8, 298
Hpy166II GTNNAC 3 cut(s) 252, 789, 1092
Hpy188I TCNGA 5 cut(s) 459, 848, 972, 1013, 1071
Hpy188III TCNNGA 4 cut(s) 136, 195, 863, 1030
Hpy8I GTNNAC 3 cut(s) 252, 789, 1092
Hpy99I CGWCG 2 cut(s) 230, 491
HpyAV CCTTC 5 cut(s) 18, 142, 790, 994, 1117
HpyCH4III ACNGT 5 cut(s) 286, 720, 774, 898, 952
HpyCH4IV ACGT 1 cut(s) 583
HpyCH4V TGCA 4 cut(s) 53, 322, 335, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 924, 1139
HpyF3I CTNAG 5 cut(s) 261, 458, 626, 647, 875
HpySE526I ACGT 1 cut(s) 583
Hsp92II CATG 5 cut(s) 349, 523, 691, 708, 1122
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 2 cut(s) 288, 427
Kzo9I GATC 5 cut(s) 41, 216, 288, 427, 700
LmnI GCTCC 1 cut(s) 394
Lsp1109I GCAGC 1 cut(s) 384
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 408
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 583
MaeIII GTNAC 2 cut(s) 587, 1041
MalI GATC 5 cut(s) 43, 218, 290, 429, 702
MboI GATC 5 cut(s) 41, 216, 288, 427, 700
MboII GAAGA 7 cut(s) 24, 93, 96, 349, 523, 562, 1033
MfeI CAATTG 1 cut(s) 961
MflI RGATCY 2 cut(s) 41, 700
MhlI GDGCHC 2 cut(s) 246, 389
MlsI TGGCCA 1 cut(s) 1098
MluCI AATT 3 cut(s) 466, 813, 961
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 2 cut(s) 257, 888
MmeI TCCRAC 4 cut(s) 549, 777, 950, 1091
Mox20I TGGCCA 1 cut(s) 1098
MroXI GAANNNNTTC 3 cut(s) 515, 635, 1025
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 4 cut(s) 722, 816, 1059, 1065
MslI CAYNNNNRTG 5 cut(s) 296, 344, 539, 614, 686
Msp20I TGGCCA 1 cut(s) 1098
MspI CCGG 4 cut(s) 445, 451, 810, 1130
MspR9I CCNGG 4 cut(s) 167, 308, 410, 527
MunI CAATTG 1 cut(s) 961
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 4 cut(s) 167, 308, 410, 527
MwoI GCNNNNNNNGC 2 cut(s) 924, 1139
NcoI CCATGG 1 cut(s) 704
NdeII GATC 5 cut(s) 41, 216, 288, 427, 700
NlaIII CATG 5 cut(s) 349, 523, 691, 708, 1122
NlaIV GGNNCC 5 cut(s) 6, 43, 449, 632, 1128
NmeAIII GCCGAG 1 cut(s) 1102
NmuCI GTSAC 1 cut(s) 1041
NspI RCATGY 1 cut(s) 691
OliI CACNNNNGTG 1 cut(s) 614
PaeR7I CTCGAG 1 cut(s) 245
PciI ACATGT 1 cut(s) 687
PctI GAATGC 1 cut(s) 337
PdmI GAANNNNTTC 3 cut(s) 515, 635, 1025
PfeI GAWTC 2 cut(s) 735, 1083
PinAI ACCGGT 1 cut(s) 444
PkrI GCNGC 1 cut(s) 399
PleI GAGTC 2 cut(s) 256, 887
PpsI GAGTC 2 cut(s) 256, 887
PscI ACATGT 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 4 cut(s) 165, 306, 408, 525
PspGI CCWGG 4 cut(s) 165, 306, 408, 525
PspN4I GGNNCC 5 cut(s) 6, 43, 449, 632, 1128
PspXI VCTCGAGB 1 cut(s) 245
PsuI RGATCY 2 cut(s) 41, 700
RsaI GTAC 3 cut(s) 788, 949, 990
RsaNI GTAC 3 cut(s) 787, 948, 989
RseI CAYNNNNRTG 5 cut(s) 296, 344, 539, 614, 686
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 4 cut(s) 722, 816, 1059, 1065
SatI GCNGC 1 cut(s) 398
Sau3AI GATC 5 cut(s) 41, 216, 288, 427, 700
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 2 cut(s) 257, 888
ScrFI CCNGG 4 cut(s) 167, 308, 410, 527
SduI GDGCHC 2 cut(s) 246, 389
SexAI ACCWGGT 1 cut(s) 408
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 245
SlaI CTCGAG 1 cut(s) 245
SmiMI CAYNNNNRTG 5 cut(s) 296, 344, 539, 614, 686
SmlI CTYRAG 2 cut(s) 245, 362
SmoI CTYRAG 2 cut(s) 245, 362
Sse9I AATT 3 cut(s) 466, 813, 961
SsiI CCGC 3 cut(s) 122, 422, 1140
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 4 cut(s) 165, 306, 408, 525
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 5 cut(s) 286, 720, 774, 898, 952
TaiI ACGT 1 cut(s) 586
TaqI TCGA 5 cut(s) 144, 225, 246, 351, 402
TasI AATT 3 cut(s) 466, 813, 961
TatI WGTACW 2 cut(s) 947, 988
TfiI GAWTC 2 cut(s) 735, 1083
Tru1I TTAA 4 cut(s) 722, 816, 1059, 1065
Tru9I TTAA 4 cut(s) 722, 816, 1059, 1065
TscAI CASTG 5 cut(s) 324, 616, 777, 913, 1094
TseFI GTSAC 1 cut(s) 1041
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 1 cut(s) 1041
TspDTI ATGAA 3 cut(s) 508, 523, 672
TspGWI ACGGA 2 cut(s) 620, 698
TspRI CASTG 5 cut(s) 324, 616, 777, 913, 1094
XceI RCATGY 1 cut(s) 691
XhoI CTCGAG 1 cut(s) 245
XmnI GAANNNNTTC 3 cut(s) 515, 635, 1025
XspI CTAG 1 cut(s) 234
ZrmI AGTACT 1 cut(s) 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.