Rh1CG179400

At1g28695-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0002782
Physical Location & Seq
Forward (+)
39660017 .. 39664171
4155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG179400.1

Sequence Viewer

Length: 894 bp
ATGGAGAACAAGACAGTGATCATAGCCGTGATAAACCAGGCTTACGCAGTGCAAGATGTGAACGCAGACACAACCATGCTCGACCTCTTCCTTGAGAGCTTTTGGCAAGGGGAGGGCACGAGGGAGCTTCTCGACCACCTGGTTCTGGTGGCGGTTGATCAGACGGCCTACGACCGGTGCCGGTTTCTGAGGCTGAATTGCTACAGATTGGAAACCGACGGCGTAGATTTCGGGGGAGAGAAGATTTTCATGTCCCAGGATTTCATCAAAATGATGTGGAAGAGAACTTGGTTCGTGTTGGAGGTTCTCAAACGTGGTTACAGCTTCATATTCACGGACACTGATGTGATGTGGCTAAGGAACCCATTCACAAAGCTAAGTAAAAATGAAACTGAGGATTTACAAATAAGCACTGACATGTTCTTTGGAGATCCATGGAACACAACACAGTTAATCAATACCGGCTTCTACTACATCAAATCCAACAACAAGACCATTGCACTGTTTGATAAATGGTACACCTTGAAGGATAATGCCACCGGAATTAAAGAACAGGATGTATTACTAAACCTAATCCGAGGTGGTATTGTTCCAGAGTTAGGTCTTAGAGTCAGGTTCTTGGATACTGTATATTTCAGTGGTTTTTGCCAGAATAGCAGGGATGTTAGGGAAGTCAGAACTGTCCATTCCAATTGTTGCCGGAGTATTGTTGCGAAAGTACAAGATTTGAAGGCTGTTCTCCGGGATTGGAAGAAGTTCAAGAAAGTAGTGTCACATACGAGGAGAGTTAATCTTAATCTGACGGCCGAAAGATTCCGGTGGACCGGCCATTGGGGATGTTGGAACTCATGGAAGGTTCCGGCAAACACCGCCAAAGCAACTCATAAGTCCTAA

Protein Analysis

297

Amino Acids

34.77

Weight (kDa)

9.04

Isoelectric Point (pI)

26.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 43 - 243 2.7e-60 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 177
AciI CCGC 2 cut(s) 152, 870
AclWI GGATC 1 cut(s) 425
AcoI YGGCCR 2 cut(s) 804, 826
AfaI GTAC 2 cut(s) 518, 720
AfiI CCNNNNNNNGG 4 cut(s) 145, 174, 599, 831
AflIII ACRYGT 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 174
AgsI TTSAA 3 cut(s) 526, 730, 760
AjnI CCWGG 3 cut(s) 36, 138, 255
AleI CACNNNNGTG 1 cut(s) 344
AluBI AGCT 4 cut(s) 99, 127, 324, 376
AluI AGCT 4 cut(s) 99, 127, 324, 376
AlwI GGATC 1 cut(s) 425
AoxI GGCC 3 cut(s) 165, 804, 826
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AsiGI ACCGGT 1 cut(s) 174
Asp700I GAANNNNTTC 3 cut(s) 245, 365, 755
AspS9I GGNCC 1 cut(s) 822
AsuC2I CCSGG 1 cut(s) 743
AvaII GGWCC 1 cut(s) 822
BaeGI GKGCMC 1 cut(s) 119
BanI GGYRCC 1 cut(s) 177
BauI CACGAG 1 cut(s) 118
BceAI ACGGC 4 cut(s) 11, 180, 235, 819
BciT130I CCWGG 3 cut(s) 38, 140, 257
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 2 cut(s) 18, 157
BcnI CCSGG 1 cut(s) 743
BfmI CTRYAG 1 cut(s) 202
BfuI GTATCC 1 cut(s) 616
Bme1390I CCNGG 4 cut(s) 38, 140, 257, 743
Bme18I GGWCC 1 cut(s) 822
BmgT120I GGNCC 1 cut(s) 822
BmiI GGNNCC 3 cut(s) 179, 362, 858
BmrFI CCNGG 4 cut(s) 38, 140, 257, 743
Bpu10I CCTNAGC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 743
BsaJI CCNNGG 3 cut(s) 255, 434, 577
BsaWI WCCGGW 3 cut(s) 174, 539, 816
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 174, 599, 831
Bse118I RCCGGY 4 cut(s) 174, 180, 461, 824
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 3 cut(s) 38, 140, 257
BseDI CCNNGG 3 cut(s) 255, 434, 577
BseGI GGATG 3 cut(s) 562, 667, 842
BseLI CCNNNNNNNGG 4 cut(s) 145, 174, 599, 831
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 2 cut(s) 179, 384
BseRI GAGGAG 1 cut(s) 796
BseSI GKGCMC 1 cut(s) 119
BseX3I CGGCCG 1 cut(s) 804
Bsh1285I CGRYCG 2 cut(s) 175, 807
BshFI GGCC 3 cut(s) 167, 806, 828
BshNI GGYRCC 1 cut(s) 177
BshTI ACCGGT 1 cut(s) 174
BsiEI CGRYCG 2 cut(s) 175, 807
BsiSI CCGG 9 cut(s) 175, 181, 462, 540, 700, 742, 817, 825, 860
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 4 cut(s) 145, 174, 599, 831
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 3 cut(s) 167, 806, 828
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 3 cut(s) 18, 157, 430
Bsp19I CCATGG 1 cut(s) 434
BspACI CCGC 2 cut(s) 152, 870
BspANI GGCC 3 cut(s) 167, 806, 828
BspCNI CTCAG 2 cut(s) 180, 385
BspLI GGNNCC 3 cut(s) 179, 362, 858
BspPI GGATC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 4 cut(s) 174, 180, 461, 824
BssAI RCCGGY 4 cut(s) 174, 180, 461, 824
BssECI CCNNGG 3 cut(s) 255, 434, 577
BssMI GATC 3 cut(s) 18, 157, 430
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 434
Bst2BI CACGAG 1 cut(s) 118
Bst2UI CCWGG 3 cut(s) 38, 140, 257
Bst4CI ACNGT 5 cut(s) 16, 450, 504, 628, 682
Bst6I CTCTTC 2 cut(s) 92, 275
BstDEI CTNAG 5 cut(s) 188, 356, 377, 393, 605
BstDSI CCRYGG 1 cut(s) 434
BstF5I GGATG 3 cut(s) 562, 667, 842
BstKTI GATC 3 cut(s) 21, 160, 433
BstMBI GATC 3 cut(s) 18, 157, 430
BstMCI CGRYCG 2 cut(s) 175, 807
BstMWI GCNNNNNNNGC 2 cut(s) 654, 869
BstNI CCWGG 3 cut(s) 38, 140, 257
BstNSI RCATGY 1 cut(s) 421
BstSCI CCNGG 4 cut(s) 36, 138, 255, 741
BstSFI CTRYAG 1 cut(s) 202
BstSLI GKGCMC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 804
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 3 cut(s) 167, 806, 828
BtgI CCRYGG 1 cut(s) 434
BtsCI GGATG 3 cut(s) 562, 667, 842
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 6 cut(s) 21, 54, 339, 411, 500, 643
Cfr10I RCCGGY 4 cut(s) 174, 180, 461, 824
Cfr13I GGNCC 1 cut(s) 822
CsiI ACCWGGT 1 cut(s) 138
Csp6I GTAC 2 cut(s) 517, 719
CspAI ACCGGT 1 cut(s) 174
CviAII CATG 5 cut(s) 76, 250, 418, 435, 849
CviQI GTAC 2 cut(s) 517, 719
DdeI CTNAG 5 cut(s) 188, 356, 377, 393, 605
DpnI GATC 3 cut(s) 20, 159, 432
DpnII GATC 3 cut(s) 18, 157, 430
EaeI YGGCCR 2 cut(s) 804, 826
EagI CGGCCG 1 cut(s) 804
Eam1104I CTCTTC 2 cut(s) 92, 275
EarI CTCTTC 2 cut(s) 92, 275
EclXI CGGCCG 1 cut(s) 804
Eco130I CCWWGG 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 822
Eco52I CGGCCG 1 cut(s) 804
EcoRII CCWGG 3 cut(s) 36, 138, 255
EcoT14I CCWWGG 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 434
FaeI CATG 5 cut(s) 79, 253, 421, 438, 852
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 75, 249, 417, 434, 848
FbaI TGATCA 2 cut(s) 18, 157
FokI GGATG 3 cut(s) 569, 674, 849
HaeIII GGCC 3 cut(s) 167, 806, 828
HapII CCGG 9 cut(s) 175, 181, 462, 540, 700, 742, 817, 825, 860
Hin1II CATG 5 cut(s) 79, 253, 421, 438, 852
HinfI GANTC 2 cut(s) 609, 813
HpaII CCGG 9 cut(s) 175, 181, 462, 540, 700, 742, 817, 825, 860
Hpy166II GTNNAC 3 cut(s) 61, 519, 822
Hpy188I TCNGA 5 cut(s) 162, 189, 578, 677, 801
Hpy188III TCNNGA 3 cut(s) 131, 593, 760
Hpy8I GTNNAC 3 cut(s) 61, 519, 822
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 3 cut(s) 520, 724, 847
HpyCH4III ACNGT 5 cut(s) 16, 450, 504, 628, 682
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 2 cut(s) 52, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 654, 869
HpyF3I CTNAG 5 cut(s) 188, 356, 377, 393, 605
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 5 cut(s) 79, 253, 421, 438, 852
Ksp22I TGATCA 2 cut(s) 18, 157
Kzo9I GATC 3 cut(s) 18, 157, 430
LmnI GCTCC 1 cut(s) 124
MabI ACCWGGT 1 cut(s) 138
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 317, 771
MalI GATC 3 cut(s) 20, 159, 432
MboI GATC 3 cut(s) 18, 157, 430
MboII GAAGA 4 cut(s) 79, 253, 292, 763
MfeI CAATTG 1 cut(s) 691
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 3 cut(s) 196, 543, 691
MlyI GAGTC 1 cut(s) 618
MmeI TCCRAC 3 cut(s) 279, 507, 821
MnlI CCTC 8 cut(s) 95, 106, 114, 183, 295, 388, 572, 774
MroXI GAANNNNTTC 3 cut(s) 245, 365, 755
MseI TTAA 4 cut(s) 452, 546, 789, 795
MslI CAYNNNNRTG 5 cut(s) 26, 74, 269, 344, 416
MspI CCGG 9 cut(s) 175, 181, 462, 540, 700, 742, 817, 825, 860
MspR9I CCNGG 4 cut(s) 38, 140, 257, 743
MunI CAATTG 1 cut(s) 691
MvaI CCWGG 3 cut(s) 38, 140, 257
MwoI GCNNNNNNNGC 2 cut(s) 654, 869
NciI CCSGG 1 cut(s) 743
NcoI CCATGG 1 cut(s) 434
NdeII GATC 3 cut(s) 18, 157, 430
NlaIII CATG 5 cut(s) 79, 253, 421, 438, 852
NlaIV GGNNCC 3 cut(s) 179, 362, 858
NmuCI GTSAC 1 cut(s) 771
NspI RCATGY 1 cut(s) 421
OliI CACNNNNGTG 1 cut(s) 344
PciI ACATGT 1 cut(s) 417
PdmI GAANNNNTTC 3 cut(s) 245, 365, 755
PfeI GAWTC 1 cut(s) 813
PfoI TCCNGGA 1 cut(s) 741
PinAI ACCGGT 1 cut(s) 174
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
PscI ACATGT 1 cut(s) 417
Psp6I CCWGG 3 cut(s) 36, 138, 255
PspGI CCWGG 3 cut(s) 36, 138, 255
PspN4I GGNNCC 3 cut(s) 179, 362, 858
PspPI GGNCC 1 cut(s) 822
PsuI RGATCY 1 cut(s) 430
RsaI GTAC 2 cut(s) 518, 720
RsaNI GTAC 2 cut(s) 517, 719
RseI CAYNNNNRTG 5 cut(s) 26, 74, 269, 344, 416
SaqAI TTAA 4 cut(s) 452, 546, 789, 795
Sau3AI GATC 3 cut(s) 18, 157, 430
Sau96I GGNCC 1 cut(s) 822
SchI GAGTC 1 cut(s) 618
ScrFI CCNGG 4 cut(s) 38, 140, 257, 743
SduI GDGCHC 1 cut(s) 119
SexAI ACCWGGT 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 822
SmiMI CAYNNNNRTG 5 cut(s) 26, 74, 269, 344, 416
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 3 cut(s) 196, 543, 691
SsiI CCGC 2 cut(s) 152, 870
StyD4I CCNGG 4 cut(s) 36, 138, 255, 741
StyI CCWWGG 1 cut(s) 434
TaaI ACNGT 5 cut(s) 16, 450, 504, 628, 682
TaiI ACGT 1 cut(s) 316
TaqI TCGA 2 cut(s) 81, 132
TasI AATT 3 cut(s) 196, 543, 691
TatI WGTACW 1 cut(s) 718
TfiI GAWTC 1 cut(s) 813
Tru1I TTAA 4 cut(s) 452, 546, 789, 795
Tru9I TTAA 4 cut(s) 452, 546, 789, 795
TscAI CASTG 6 cut(s) 21, 54, 346, 418, 507, 643
TseFI GTSAC 1 cut(s) 771
Tsp45I GTSAC 1 cut(s) 771
TspDTI ATGAA 4 cut(s) 238, 253, 316, 402
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 6 cut(s) 21, 54, 346, 418, 507, 643
VpaK11BI GGWCC 1 cut(s) 822
XceI RCATGY 1 cut(s) 421
XmnI GAANNNNTTC 3 cut(s) 245, 365, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.