Rroxscaffold_4G00309180

At1g28695-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002782
Physical Location & Seq
Reverse (-)
30850088 .. 30852896
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309180.1

Sequence Viewer

Length: 1119 bp
ATGGAACCTTCTCATCACCAGAAACATCACTCTTCTTTGGGATCCAATCTTTCATTTCTCTCTCTACTCCTCGCTTGTGTTGTTTATCTCTCTATATGGTCTTCTTCCTCCTCTATGAACCCGCTTTTCGCCTTCCAGAAACACGATGAGGGCGCATCACAAAGCCTGAAGATCACATTCGACGAGCCTCAAGACGAGCTCGAGTCCACTTTATCCAAGGCTTCAATGGAGAACAAGACAGTGATCATAGCCGTGATAAACCAGGCTTACGCAGTACAAGATGTGAACGCAGACACAACCATGCTCGACCTCTTCCTTGAGAGCTTTTGGCAAGGGGAGGGCACGAGGGAGCTGCTCGACCACCTGGTTCTGGTGGCGGTTGATCAGACTGCCTACGACCGGTGCCGGTTTCTGAGGCTGAATTGCTACAGATTGGAAACCGACGGCGTAGATTTCGGGGGAGAGAAGATTTTCATGTCCCAGGATTTCATCAAAATGATGTGGAAGAGAACTTGGTTCGTGTTGGAGGTTCTCAAACGCGGTTACAGCTTCGTATTCACGGACACTGATGTGATGTGGCTAAGGAACCCATTCACAAAGCTAAGTAAAAATGAAACCGAGGATTTACAAATAAGCACTGACATGTTCTTTGGAGATCCATGGAACACAACACAGTTAATCAATACTGGATTCTACTACATCAAATCCAACAACAAGACCATTGCACTGTTTGATAAATGGTACACCTTGAAGGACAATGCCACTGGAATTAAAGAACAGGATGTATTACTAAACCTAATCCGAAGTGGTATTGTTCCAGAGTTAGGTCTTAGAGTCAGGTTCTTGGATACTGTATATTTCAGTGGTTTTTGCCAGAATAGCAGGGATATTAGGGAAGTCAGTACTGTCCATTCCAATTGTTGCCGGAGTATTGTTGCGAAAGTACAAGATTTGAAGACTGTTCTCCGAGATTGGAAGAAGTTCAAGAAAGTAGTGTCACATACGAGGAGGGTTAATCTTAATCTGACGGCCGAGAGATTCCAGTGGACCGGCCATTGGGGATGTTGGAACTCATGGAAGGTTCCGGCAAACACCGCCAAAGCAACTCATAAGTCCTAA

Protein Analysis

372

Amino Acids

43.0

Weight (kDa)

7.63

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 118 - 318 7.9e-60 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 402
AccII CGCG 1 cut(s) 540
AciI CCGC 4 cut(s) 122, 377, 540, 1095
AclWI GGATC 3 cut(s) 36, 49, 650
AcoI YGGCCR 2 cut(s) 1029, 1051
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 4 cut(s) 276, 743, 904, 945
AfiI CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
AflIII ACRYGT 1 cut(s) 642
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 4 cut(s) 225, 751, 955, 985
AjnI CCWGG 3 cut(s) 261, 363, 480
AleI CACNNNNGTG 1 cut(s) 569
AluBI AGCT 5 cut(s) 199, 324, 352, 549, 601
AluI AGCT 5 cut(s) 199, 324, 352, 549, 601
Alw21I GWGCWC 1 cut(s) 201
AlwI GGATC 3 cut(s) 36, 49, 650
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 2 cut(s) 1029, 1051
ApeKI GCWGC 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AsiGI ACCGGT 1 cut(s) 399
Asp700I GAANNNNTTC 3 cut(s) 470, 590, 980
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 1047
AsuHPI GGTGA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 1047
BaeGI GKGCMC 1 cut(s) 344
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 201
BauI CACGAG 1 cut(s) 343
BbsI GAAGAC 2 cut(s) 93, 962
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 339
BceAI ACGGC 3 cut(s) 236, 460, 1044
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 3 cut(s) 263, 365, 482
BciVI GTATCC 1 cut(s) 841
BclI TGATCA 2 cut(s) 243, 382
BfmI CTRYAG 1 cut(s) 427
BfuI GTATCC 1 cut(s) 841
BisI GCNGC 1 cut(s) 353
BlsI GCNGC 1 cut(s) 354
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 3 cut(s) 263, 365, 482
Bme18I GGWCC 1 cut(s) 1047
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 1047
BmiI GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
BmrFI CCNGG 3 cut(s) 263, 365, 482
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 2 cut(s) 93, 962
Bpu10I CCTNAGC 1 cut(s) 581
BpuEI CTTGAG 2 cut(s) 174, 338
BsaJI CCNNGG 4 cut(s) 216, 480, 618, 659
BsaWI WCCGGW 1 cut(s) 399
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
Bse118I RCCGGY 3 cut(s) 399, 405, 1049
Bse1I ACTGG 3 cut(s) 691, 769, 1042
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 3 cut(s) 263, 365, 482
BseDI CCNNGG 4 cut(s) 216, 480, 618, 659
BseGI GGATG 2 cut(s) 787, 1067
BseLI CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 3 cut(s) 691, 769, 1042
BseRI GAGGAG 3 cut(s) 59, 100, 1021
BseSI GKGCMC 1 cut(s) 344
BseX3I CGGCCG 1 cut(s) 1029
BseXI GCAGC 1 cut(s) 339
Bsh1236I CGCG 1 cut(s) 540
Bsh1285I CGRYCG 2 cut(s) 400, 1032
BshFI GGCC 2 cut(s) 1031, 1053
BshNI GGYRCC 1 cut(s) 402
BshTI ACCGGT 1 cut(s) 399
BsiEI CGRYCG 2 cut(s) 400, 1032
BsiHKAI GWGCWC 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 5 cut(s) 400, 406, 925, 1050, 1085
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
BsmFI GGGAC 1 cut(s) 463
BsnI GGCC 2 cut(s) 1031, 1053
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 201, 344
Bsp143I GATC 5 cut(s) 41, 171, 243, 382, 655
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 4 cut(s) 122, 377, 540, 1095
BspANI GGCC 2 cut(s) 1031, 1053
BspCNI CTCAG 1 cut(s) 405
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
BspPI GGATC 3 cut(s) 36, 49, 650
BspT107I GGYRCC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 3 cut(s) 399, 405, 1049
BsrI ACTGG 3 cut(s) 691, 769, 1042
BssAI RCCGGY 3 cut(s) 399, 405, 1049
BssECI CCNNGG 4 cut(s) 216, 480, 618, 659
BssMI GATC 5 cut(s) 41, 171, 243, 382, 655
BssSI CACGAG 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 216, 659
Bst2BI CACGAG 1 cut(s) 343
Bst2UI CCWGG 3 cut(s) 263, 365, 482
Bst4CI ACNGT 6 cut(s) 241, 675, 729, 853, 907, 961
Bst6I CTCTTC 3 cut(s) 37, 317, 500
BstDEI CTNAG 4 cut(s) 413, 581, 602, 830
BstDSI CCRYGG 1 cut(s) 659
BstF5I GGATG 2 cut(s) 787, 1067
BstFNI CGCG 1 cut(s) 540
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 5 cut(s) 44, 174, 246, 385, 658
BstMBI GATC 5 cut(s) 41, 171, 243, 382, 655
BstMCI CGRYCG 2 cut(s) 400, 1032
BstMWI GCNNNNNNNGC 3 cut(s) 546, 879, 1094
BstNI CCWGG 3 cut(s) 263, 365, 482
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 261, 363, 480
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 1 cut(s) 339
BstV2I GAAGAC 2 cut(s) 93, 962
BstX2I RGATCY 2 cut(s) 41, 655
BstYI RGATCY 2 cut(s) 41, 655
BstZI CGGCCG 1 cut(s) 1029
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 2 cut(s) 1031, 1053
BtgI CCRYGG 1 cut(s) 659
BtsCI GGATG 2 cut(s) 787, 1067
BtsIMutI CAGTG 7 cut(s) 246, 564, 636, 725, 762, 868, 1049
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 3 cut(s) 399, 405, 1049
Cfr13I GGNCC 1 cut(s) 1047
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 4 cut(s) 275, 742, 903, 944
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 5 cut(s) 301, 475, 643, 660, 1074
CviQI GTAC 4 cut(s) 275, 742, 903, 944
DdeI CTNAG 4 cut(s) 413, 581, 602, 830
DpnI GATC 5 cut(s) 43, 173, 245, 384, 657
DpnII GATC 5 cut(s) 41, 171, 243, 382, 655
EaeI YGGCCR 2 cut(s) 1029, 1051
EagI CGGCCG 1 cut(s) 1029
Eam1104I CTCTTC 3 cut(s) 37, 317, 500
EarI CTCTTC 3 cut(s) 37, 317, 500
Ecl136II GAGCTC 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 1029
Eco130I CCWWGG 2 cut(s) 216, 659
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 1047
Eco52I CGGCCG 1 cut(s) 1029
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 188
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 3 cut(s) 261, 363, 480
EcoT14I CCWWGG 2 cut(s) 216, 659
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 216, 659
FaeI CATG 5 cut(s) 304, 478, 646, 663, 1077
FaqI GGGAC 1 cut(s) 463
FatI CATG 5 cut(s) 300, 474, 642, 659, 1073
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 2 cut(s) 243, 382
Fnu4HI GCNGC 1 cut(s) 353
FokI GGATG 2 cut(s) 794, 1074
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 353
GlaI GCGC 1 cut(s) 154
GluI GCNGC 1 cut(s) 353
HaeIII GGCC 2 cut(s) 1031, 1053
HapII CCGG 5 cut(s) 400, 406, 925, 1050, 1085
HhaI GCGC 1 cut(s) 155
Hin1II CATG 5 cut(s) 304, 478, 646, 663, 1077
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 4 cut(s) 203, 690, 834, 1038
HpaII CCGG 5 cut(s) 400, 406, 925, 1050, 1085
HphI GGTGA 1 cut(s) 8
Hpy166II GTNNAC 4 cut(s) 207, 286, 744, 1047
Hpy188I TCNGA 5 cut(s) 387, 414, 803, 968, 1026
Hpy188III TCNNGA 4 cut(s) 136, 191, 818, 985
Hpy8I GTNNAC 4 cut(s) 207, 286, 744, 1047
Hpy99I CGWCG 2 cut(s) 185, 446
HpyAV CCTTC 4 cut(s) 18, 142, 745, 1072
HpyCH4III ACNGT 6 cut(s) 241, 675, 729, 853, 907, 961
HpyCH4V TGCA 1 cut(s) 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 546, 879, 1094
HpyF3I CTNAG 4 cut(s) 413, 581, 602, 830
Hsp92II CATG 5 cut(s) 304, 478, 646, 663, 1077
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 2 cut(s) 243, 382
Kzo9I GATC 5 cut(s) 41, 171, 243, 382, 655
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 1 cut(s) 339
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 542, 996
MalI GATC 5 cut(s) 43, 173, 245, 384, 657
MboI GATC 5 cut(s) 41, 171, 243, 382, 655
MboII GAAGA 9 cut(s) 24, 93, 96, 181, 304, 478, 517, 967, 988
MfeI CAATTG 1 cut(s) 916
MflI RGATCY 2 cut(s) 41, 655
MhlI GDGCHC 2 cut(s) 201, 344
MluCI AATT 3 cut(s) 421, 768, 916
MlyI GAGTC 2 cut(s) 212, 843
MmeI TCCRAC 3 cut(s) 504, 732, 1046
MroXI GAANNNNTTC 3 cut(s) 470, 590, 980
MseI TTAA 4 cut(s) 677, 771, 1014, 1020
MslI CAYNNNNRTG 5 cut(s) 251, 299, 494, 569, 641
MspI CCGG 5 cut(s) 400, 406, 925, 1050, 1085
MspR9I CCNGG 3 cut(s) 263, 365, 482
MunI CAATTG 1 cut(s) 916
MvaI CCWGG 3 cut(s) 263, 365, 482
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 3 cut(s) 546, 879, 1094
NcoI CCATGG 1 cut(s) 659
NdeII GATC 5 cut(s) 41, 171, 243, 382, 655
NlaIII CATG 5 cut(s) 304, 478, 646, 663, 1077
NlaIV GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
NmeAIII GCCGAG 1 cut(s) 1057
NmuCI GTSAC 1 cut(s) 996
NspI RCATGY 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 569
PaeR7I CTCGAG 1 cut(s) 200
PciI ACATGT 1 cut(s) 642
PdmI GAANNNNTTC 3 cut(s) 470, 590, 980
PfeI GAWTC 2 cut(s) 690, 1038
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 1 cut(s) 354
PleI GAGTC 2 cut(s) 211, 842
PpsI GAGTC 2 cut(s) 211, 842
PscI ACATGT 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 3 cut(s) 261, 363, 480
PspGI CCWGG 3 cut(s) 261, 363, 480
PspN4I GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
PspPI GGNCC 1 cut(s) 1047
PspXI VCTCGAGB 1 cut(s) 200
PsuI RGATCY 2 cut(s) 41, 655
RsaI GTAC 4 cut(s) 276, 743, 904, 945
RsaNI GTAC 4 cut(s) 275, 742, 903, 944
RseI CAYNNNNRTG 5 cut(s) 251, 299, 494, 569, 641
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 4 cut(s) 677, 771, 1014, 1020
SatI GCNGC 1 cut(s) 353
Sau3AI GATC 5 cut(s) 41, 171, 243, 382, 655
Sau96I GGNCC 1 cut(s) 1047
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 2 cut(s) 212, 843
ScrFI CCNGG 3 cut(s) 263, 365, 482
SduI GDGCHC 2 cut(s) 201, 344
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 427
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 1 cut(s) 1047
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 5 cut(s) 251, 299, 494, 569, 641
SmlI CTYRAG 3 cut(s) 189, 200, 317
SmoI CTYRAG 3 cut(s) 189, 200, 317
Sse9I AATT 3 cut(s) 421, 768, 916
SsiI CCGC 4 cut(s) 122, 377, 540, 1095
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 3 cut(s) 261, 363, 480
StyI CCWWGG 2 cut(s) 216, 659
TaaI ACNGT 6 cut(s) 241, 675, 729, 853, 907, 961
TaqI TCGA 4 cut(s) 180, 201, 306, 357
TasI AATT 3 cut(s) 421, 768, 916
TatI WGTACW 3 cut(s) 274, 902, 943
TfiI GAWTC 2 cut(s) 690, 1038
Tru1I TTAA 4 cut(s) 677, 771, 1014, 1020
Tru9I TTAA 4 cut(s) 677, 771, 1014, 1020
TscAI CASTG 7 cut(s) 246, 571, 643, 732, 769, 868, 1049
TseFI GTSAC 1 cut(s) 996
TseI GCWGC 1 cut(s) 352
Tsp45I GTSAC 1 cut(s) 996
TspDTI ATGAA 5 cut(s) 42, 131, 463, 478, 627
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 7 cut(s) 246, 571, 643, 732, 769, 868, 1049
VpaK11BI GGWCC 1 cut(s) 1047
XceI RCATGY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 200
XmnI GAANNNNTTC 3 cut(s) 470, 590, 980
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.