RchiOBHm_Chr1g0345241

At1g28695-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002782
Physical Location & Seq
Forward (+)
37625049 .. 37629697
4649 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57158

Sequence Viewer

Length: 1119 bp
ATGGAACCTTCTCATCACCAGAAACATCACTCTTCTTTGGGATCCAATCTTGCATTCCTCTCTCTACTCCTCGCTTGTGTTGTTTATCTCTCTATATGGTCTTCTTCCTCCTCTATCAACCCGCTTTTCGCCTTCCAGAAACACGATGATGGCGCATCACAAAGCCTGACGATCACATTCGACGATCCTCAAGACGAGCTCGAGTCCACTTTATCCAAGGCTTCAATGGAGAACAAGACAGTGATCATAGCCGTGATAAACCAGGCTTACGCAGTGCAAGATGTGAACGCAGACACAACCATGCTCGACCTCTTCCTTGAGAGCTTTTGGCAAGGGGAGGGCACGAGGGAGCTTCTCGACCACCTGGTTCTGGTGGCGGTTGATCAGACGGCCTACGACCGGTGCCGGTTTCTGAGGCTGAATTGCTACAGATTGGAAACCGACGGCGTAGATTTCGGGGGAGAGAAGATTTTCATGTCCCAGGATTTCATCAAAATGATGTGGAAGAGAACTTGGTTCGTGTTGGAGGTTCTCAAACGTGGTTACAGCTTCATATTCACGGACACTGATGTGATGTGGCTAAGGAACCCATTCACAAAGCTAAGTAAAAATGAAACTGAGGATTTACAAATAAGCACTGACATGTTCTTTGGAGATCCATGGAACACAACACAGTTAATCAATACCGGCTTCTACTACATCAAATCCAACAACAAGACCATTGCACTGTTTGATAAATGGTACACCTTGAAGGATAATGCCACCGGAATTAAAGAACAGGATGTATTACTAAACCTAATTCGAGGTGGTATTGTTCCAGAGTTAGGTCTTAGAGTCAGGTTCTTGGATACTGTATATTTCAGTGGTTTTTGCCAGAATAGCAGGGATGTTAGGGAAGTCAGTACTGTCCATTCCAATTGTTGCCGGAGTATTGTTGCGAAAGTACAAGATTTGAAGGCTGTTCTCCGAGATTGGAAGAAGTTCAAGAAAGTAGTGTCACATACGAGGAGGGTTAATCTTAATCTGACGGCCGAGAGATTCCGGTGGACCGGCCATTGGGGATGTTGGAACTCATGGAAGGTTCCGGCAAACACCGCCAAAGCAACTCATAAGTCCTAA

Protein Analysis

372

Amino Acids

42.88

Weight (kDa)

7.63

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nucleotid_trans PF03407 118 - 318 6e-60 Nucleotide-diphospho-sugar transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 402
AciI CCGC 3 cut(s) 122, 377, 1095
AclWI GGATC 4 cut(s) 36, 49, 179, 650
AcoI YGGCCR 2 cut(s) 1029, 1051
AfaI GTAC 3 cut(s) 743, 904, 945
AfiI CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
AflIII ACRYGT 1 cut(s) 642
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 4 cut(s) 225, 751, 955, 985
AjnI CCWGG 3 cut(s) 261, 363, 480
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
AleI CACNNNNGTG 1 cut(s) 569
AluBI AGCT 5 cut(s) 199, 324, 352, 549, 601
AluI AGCT 5 cut(s) 199, 324, 352, 549, 601
Alw21I GWGCWC 1 cut(s) 201
AlwI GGATC 4 cut(s) 36, 49, 179, 650
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 3 cut(s) 390, 1029, 1051
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AsiGI ACCGGT 1 cut(s) 399
Asp700I GAANNNNTTC 3 cut(s) 470, 590, 980
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 1047
AsuHPI GGTGA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 1047
BaeGI GKGCMC 1 cut(s) 344
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 201
BauI CACGAG 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 201
BccI CCATC 1 cut(s) 143
BceAI ACGGC 4 cut(s) 236, 405, 460, 1044
BciT130I CCWGG 3 cut(s) 263, 365, 482
BciVI GTATCC 1 cut(s) 841
BclI TGATCA 2 cut(s) 243, 382
BfmI CTRYAG 1 cut(s) 427
BfuI GTATCC 1 cut(s) 841
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 3 cut(s) 263, 365, 482
Bme18I GGWCC 1 cut(s) 1047
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 1047
BmiI GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
BmrFI CCNGG 3 cut(s) 263, 365, 482
BmsI GCATC 1 cut(s) 164
BpiI GAAGAC 1 cut(s) 93
Bpu10I CCTNAGC 1 cut(s) 581
BpuEI CTTGAG 2 cut(s) 174, 338
BsaJI CCNNGG 3 cut(s) 216, 480, 659
BsaWI WCCGGW 3 cut(s) 399, 764, 1041
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
Bse118I RCCGGY 4 cut(s) 399, 405, 686, 1049
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 3 cut(s) 263, 365, 482
BseDI CCNNGG 3 cut(s) 216, 480, 659
BseGI GGATG 3 cut(s) 787, 892, 1067
BseLI CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 404, 609
BseRI GAGGAG 3 cut(s) 59, 100, 1021
BseSI GKGCMC 1 cut(s) 344
BseX3I CGGCCG 1 cut(s) 1029
Bsh1285I CGRYCG 2 cut(s) 400, 1032
BshFI GGCC 3 cut(s) 392, 1031, 1053
BshNI GGYRCC 1 cut(s) 402
BshTI ACCGGT 1 cut(s) 399
BsiEI CGRYCG 2 cut(s) 400, 1032
BsiHKAI GWGCWC 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 8 cut(s) 400, 406, 687, 765, 925, 1042, 1050, 1085
BslFI GGGAC 1 cut(s) 463
BslI CCNNNNNNNGG 4 cut(s) 370, 399, 824, 1056
BsmFI GGGAC 1 cut(s) 463
BsmI GAATGC 1 cut(s) 53
BsnI GGCC 3 cut(s) 392, 1031, 1053
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 201, 344
Bsp143I GATC 6 cut(s) 41, 171, 184, 243, 382, 655
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 3 cut(s) 122, 377, 1095
BspANI GGCC 3 cut(s) 392, 1031, 1053
BspCNI CTCAG 2 cut(s) 405, 610
BspLI GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
BspPI GGATC 4 cut(s) 36, 49, 179, 650
BspT107I GGYRCC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 4 cut(s) 399, 405, 686, 1049
BssAI RCCGGY 4 cut(s) 399, 405, 686, 1049
BssECI CCNNGG 3 cut(s) 216, 480, 659
BssMI GATC 6 cut(s) 41, 171, 184, 243, 382, 655
BssSI CACGAG 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 216, 659
Bst2BI CACGAG 1 cut(s) 343
Bst2UI CCWGG 3 cut(s) 263, 365, 482
Bst4CI ACNGT 5 cut(s) 241, 675, 729, 853, 907
Bst6I CTCTTC 3 cut(s) 37, 317, 500
BstDEI CTNAG 5 cut(s) 413, 581, 602, 618, 830
BstDSI CCRYGG 1 cut(s) 659
BstF5I GGATG 3 cut(s) 787, 892, 1067
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 6 cut(s) 44, 174, 187, 246, 385, 658
BstMBI GATC 6 cut(s) 41, 171, 184, 243, 382, 655
BstMCI CGRYCG 2 cut(s) 400, 1032
BstMWI GCNNNNNNNGC 2 cut(s) 879, 1094
BstNI CCWGG 3 cut(s) 263, 365, 482
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 261, 363, 480
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 344
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 2 cut(s) 41, 655
BstYI RGATCY 2 cut(s) 41, 655
BstZI CGGCCG 1 cut(s) 1029
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 3 cut(s) 392, 1031, 1053
BtgI CCRYGG 1 cut(s) 659
BtsCI GGATG 3 cut(s) 787, 892, 1067
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 6 cut(s) 246, 279, 564, 636, 725, 868
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 4 cut(s) 399, 405, 686, 1049
Cfr13I GGNCC 1 cut(s) 1047
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 3 cut(s) 742, 903, 944
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 5 cut(s) 301, 475, 643, 660, 1074
CviQI GTAC 3 cut(s) 742, 903, 944
DdeI CTNAG 5 cut(s) 413, 581, 602, 618, 830
DpnI GATC 6 cut(s) 43, 173, 186, 245, 384, 657
DpnII GATC 6 cut(s) 41, 171, 184, 243, 382, 655
EaeI YGGCCR 2 cut(s) 1029, 1051
EagI CGGCCG 1 cut(s) 1029
Eam1104I CTCTTC 3 cut(s) 37, 317, 500
EarI CTCTTC 3 cut(s) 37, 317, 500
Ecl136II GAGCTC 1 cut(s) 199
EclXI CGGCCG 1 cut(s) 1029
Eco130I CCWWGG 2 cut(s) 216, 659
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 1047
Eco52I CGGCCG 1 cut(s) 1029
Eco53kI GAGCTC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 199
EcoRII CCWGG 3 cut(s) 261, 363, 480
EcoT14I CCWWGG 2 cut(s) 216, 659
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 2 cut(s) 216, 659
FaeI CATG 5 cut(s) 304, 478, 646, 663, 1077
FaqI GGGAC 1 cut(s) 463
FatI CATG 5 cut(s) 300, 474, 642, 659, 1073
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 2 cut(s) 243, 382
FokI GGATG 3 cut(s) 794, 899, 1074
FriOI GRGCYC 1 cut(s) 201
GlaI GCGC 1 cut(s) 154
HaeIII GGCC 3 cut(s) 392, 1031, 1053
HapII CCGG 8 cut(s) 400, 406, 687, 765, 925, 1042, 1050, 1085
HhaI GCGC 1 cut(s) 155
Hin1II CATG 5 cut(s) 304, 478, 646, 663, 1077
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 3 cut(s) 203, 834, 1038
HpaII CCGG 8 cut(s) 400, 406, 687, 765, 925, 1042, 1050, 1085
HphI GGTGA 1 cut(s) 8
Hpy166II GTNNAC 4 cut(s) 207, 286, 744, 1047
Hpy188I TCNGA 4 cut(s) 387, 414, 968, 1026
Hpy188III TCNNGA 5 cut(s) 136, 191, 356, 818, 985
Hpy8I GTNNAC 4 cut(s) 207, 286, 744, 1047
Hpy99I CGWCG 2 cut(s) 185, 446
HpyAV CCTTC 5 cut(s) 18, 142, 745, 949, 1072
HpyCH4III ACNGT 5 cut(s) 241, 675, 729, 853, 907
HpyCH4IV ACGT 1 cut(s) 538
HpyCH4V TGCA 3 cut(s) 53, 277, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 879, 1094
HpyF3I CTNAG 5 cut(s) 413, 581, 602, 618, 830
HpySE526I ACGT 1 cut(s) 538
Hsp92II CATG 5 cut(s) 304, 478, 646, 663, 1077
HspAI GCGC 1 cut(s) 153
Ksp22I TGATCA 2 cut(s) 243, 382
Kzo9I GATC 6 cut(s) 41, 171, 184, 243, 382, 655
LmnI GCTCC 1 cut(s) 349
LweI GCATC 1 cut(s) 164
MabI ACCWGGT 1 cut(s) 363
MaeII ACGT 1 cut(s) 538
MaeIII GTNAC 2 cut(s) 542, 996
MalI GATC 6 cut(s) 43, 173, 186, 245, 384, 657
MboI GATC 6 cut(s) 41, 171, 184, 243, 382, 655
MboII GAAGA 7 cut(s) 24, 93, 96, 304, 478, 517, 988
MfeI CAATTG 1 cut(s) 916
MflI RGATCY 2 cut(s) 41, 655
MhlI GDGCHC 2 cut(s) 201, 344
MluCI AATT 4 cut(s) 421, 768, 798, 916
MlyI GAGTC 2 cut(s) 212, 843
MmeI TCCRAC 3 cut(s) 504, 732, 1046
MroXI GAANNNNTTC 3 cut(s) 470, 590, 980
MseI TTAA 4 cut(s) 677, 771, 1014, 1020
MslI CAYNNNNRTG 6 cut(s) 147, 251, 299, 494, 569, 641
MspI CCGG 8 cut(s) 400, 406, 687, 765, 925, 1042, 1050, 1085
MspR9I CCNGG 3 cut(s) 263, 365, 482
MunI CAATTG 1 cut(s) 916
Mva1269I GAATGC 1 cut(s) 53
MvaI CCWGG 3 cut(s) 263, 365, 482
MwoI GCNNNNNNNGC 2 cut(s) 879, 1094
NcoI CCATGG 1 cut(s) 659
NdeII GATC 6 cut(s) 41, 171, 184, 243, 382, 655
NlaIII CATG 5 cut(s) 304, 478, 646, 663, 1077
NlaIV GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
NmeAIII GCCGAG 1 cut(s) 1057
NmuCI GTSAC 1 cut(s) 996
NspI RCATGY 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 569
PaeR7I CTCGAG 1 cut(s) 200
PciI ACATGT 1 cut(s) 642
PctI GAATGC 1 cut(s) 53
PdmI GAANNNNTTC 3 cut(s) 470, 590, 980
PfeI GAWTC 1 cut(s) 1038
PinAI ACCGGT 1 cut(s) 399
PleI GAGTC 2 cut(s) 211, 842
PpsI GAGTC 2 cut(s) 211, 842
PscI ACATGT 1 cut(s) 642
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 3 cut(s) 261, 363, 480
PspGI CCWGG 3 cut(s) 261, 363, 480
PspN4I GGNNCC 5 cut(s) 6, 43, 404, 587, 1083
PspPI GGNCC 1 cut(s) 1047
PspXI VCTCGAGB 1 cut(s) 200
PsuI RGATCY 2 cut(s) 41, 655
RsaI GTAC 3 cut(s) 743, 904, 945
RsaNI GTAC 3 cut(s) 742, 903, 944
RseI CAYNNNNRTG 6 cut(s) 147, 251, 299, 494, 569, 641
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 4 cut(s) 677, 771, 1014, 1020
Sau3AI GATC 6 cut(s) 41, 171, 184, 243, 382, 655
Sau96I GGNCC 1 cut(s) 1047
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 2 cut(s) 212, 843
ScrFI CCNGG 3 cut(s) 263, 365, 482
SduI GDGCHC 2 cut(s) 201, 344
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 1 cut(s) 164
SfcI CTRYAG 1 cut(s) 427
Sfr274I CTCGAG 1 cut(s) 200
SinI GGWCC 1 cut(s) 1047
SlaI CTCGAG 1 cut(s) 200
SmiMI CAYNNNNRTG 6 cut(s) 147, 251, 299, 494, 569, 641
SmlI CTYRAG 3 cut(s) 189, 200, 317
SmoI CTYRAG 3 cut(s) 189, 200, 317
Sse9I AATT 4 cut(s) 421, 768, 798, 916
SsiI CCGC 3 cut(s) 122, 377, 1095
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 3 cut(s) 261, 363, 480
StyI CCWWGG 2 cut(s) 216, 659
TaaI ACNGT 5 cut(s) 241, 675, 729, 853, 907
TaiI ACGT 1 cut(s) 541
TaqI TCGA 5 cut(s) 180, 201, 306, 357, 802
TasI AATT 4 cut(s) 421, 768, 798, 916
TatI WGTACW 2 cut(s) 902, 943
TfiI GAWTC 1 cut(s) 1038
Tru1I TTAA 4 cut(s) 677, 771, 1014, 1020
Tru9I TTAA 4 cut(s) 677, 771, 1014, 1020
TscAI CASTG 6 cut(s) 246, 279, 571, 643, 732, 868
TseFI GTSAC 1 cut(s) 996
Tsp45I GTSAC 1 cut(s) 996
TspDTI ATGAA 4 cut(s) 463, 478, 541, 627
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 6 cut(s) 246, 279, 571, 643, 732, 868
VpaK11BI GGWCC 1 cut(s) 1047
XceI RCATGY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 200
XmnI GAANNNNTTC 3 cut(s) 470, 590, 980
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.