FvH4_7g16550

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001280
Physical Location & Seq
Forward (+)
14185907 .. 14186606
700 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16550.t1

Sequence Viewer

Length: 516 bp
ATGGAGGAAGATTGGCAGCAACCGAAATGGGAAGGCAAAACATCAGCTGAGCTGAAAGGCACGGCAGCAGAAGAGGTCTGGCCTCTCTTGGCAGATTTCTGCAACATATACAAGTGGTTCCAAAGTCTTGAGATCAGTTATCATGTGGAGGGAGTCCCCGGCCAACCTGGCCTGATTCGCTACTGCGGGCACCGCGACCGTGGTGATCATGACGAAGATGATCAGACGTCGGAGCCGATCAAATTCTCTGCCAAAGAGAAGCTGCTGATGATCGATCCAGTCAGGCGGTGTTTGAGCTACGAGATACTAGACAACAATTGGGGGCTCAAGTCATATGTTGCAACCATGCAAGTGTTGGATTTGGATGGGTGCCAAAGCCGCCGTGGGTGCAAGATTGAGTGGTCGTTTGTTTCTGATCCTATTGAAGGATGGAGGTTTGGAGATTACGTATCTTATCTCCACTCTTGTCTGCAATCCATGGCAAAAAAGATGGAGCATGCTACCGATGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

172

Amino Acids

19.75

Weight (kDa)

4.93

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Polyketide_cyc2 PF10604 15 - 149 3.8e-13 Polyketide cyclase / dehydrase and lipid transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 230
AccB1I GGYRCC 2 cut(s) 189, 369
AccII CGCG 1 cut(s) 195
AciI CCGC 4 cut(s) 186, 193, 286, 379
AclWI GGATC 2 cut(s) 269, 410
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 425
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 5 cut(s) 47, 52, 262, 297, 513
AluI AGCT 5 cut(s) 47, 52, 262, 297, 513
AlwI GGATC 2 cut(s) 269, 410
AoxI GGCC 3 cut(s) 80, 160, 169
ApeKI GCWGC 3 cut(s) 16, 65, 262
ApoI RAATTY 1 cut(s) 242
AsuC2I CCSGG 1 cut(s) 159
AsuHPI GGTGA 1 cut(s) 215
AsuNHI GCTAGC 1 cut(s) 509
BaeGI GKGCMC 1 cut(s) 192
BanI GGYRCC 2 cut(s) 189, 369
BanII GRGCYC 1 cut(s) 327
BbvI GCAGC 3 cut(s) 28, 77, 249
BccI CCATC 3 cut(s) 359, 423, 484
BceAI ACGGC 2 cut(s) 78, 366
BciT130I CCWGG 1 cut(s) 168
BclI TGATCA 2 cut(s) 205, 220
BcnI CCSGG 1 cut(s) 159
BfaI CTAG 2 cut(s) 308, 510
BglI GCCNNNNNGGC 1 cut(s) 168
BisI GCNGC 4 cut(s) 17, 66, 263, 379
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 4 cut(s) 18, 67, 264, 380
Bme1390I CCNGG 2 cut(s) 159, 168
BmiI GGNNCC 4 cut(s) 119, 191, 234, 371
BmrFI CCNGG 2 cut(s) 159, 168
BmsI GCATC 1 cut(s) 496
BmtI GCTAGC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 149, 311
BpuMI CCSGG 1 cut(s) 159
Bsa29I ATCGAT 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 448
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 4 cut(s) 157, 199, 382, 477
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 4 cut(s) 157, 199, 382, 477
BseGI GGATG 2 cut(s) 370, 434
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 3 cut(s) 28, 77, 249
Bsh1236I CGCG 1 cut(s) 195
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 3 cut(s) 82, 162, 171
BshNI GGYRCC 2 cut(s) 189, 369
BshVI ATCGAT 1 cut(s) 273
BsiEI CGRYCG 1 cut(s) 199
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 425
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 3 cut(s) 82, 162, 171
Bsp1286I GDGCHC 2 cut(s) 192, 327
Bsp143I GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
Bsp1720I GCTNAGC 1 cut(s) 48
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 4 cut(s) 186, 193, 286, 379
BspANI GGCC 3 cut(s) 82, 162, 171
BspCNI CTCAG 1 cut(s) 40
BspDI ATCGAT 1 cut(s) 273
BspFNI CGCG 1 cut(s) 195
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 4 cut(s) 119, 191, 234, 371
BspOI GCTAGC 1 cut(s) 513
BspPI GGATC 2 cut(s) 269, 410
BspT107I GGYRCC 2 cut(s) 189, 369
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 4 cut(s) 157, 199, 382, 477
BssMI GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
BssNI GRCGYC 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 66
BstACI GRCGYC 1 cut(s) 227
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 3 cut(s) 188, 498, 511
BstDEI CTNAG 1 cut(s) 48
BstDSI CCRYGG 3 cut(s) 199, 382, 477
BstENI CCTNNNNNAGG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 370, 434
BstFNI CGCG 1 cut(s) 195
BstKTI GATC 7 cut(s) 135, 208, 223, 240, 273, 277, 418
BstMBI GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 6 cut(s) 168, 177, 192, 378, 387, 506
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 2 cut(s) 157, 166
BstSLI GKGCMC 1 cut(s) 192
BstSNI TACGTA 1 cut(s) 448
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 3 cut(s) 28, 77, 249
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 3 cut(s) 82, 162, 171
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 3 cut(s) 199, 382, 477
BtsCI GGATG 2 cut(s) 370, 434
Cac8I GCNNGC 3 cut(s) 188, 498, 511
CciI TCATGA 1 cut(s) 208
ClaI ATCGAT 1 cut(s) 273
CviAII CATG 5 cut(s) 143, 209, 346, 478, 497
DdeI CTNAG 1 cut(s) 48
DpnI GATC 7 cut(s) 134, 207, 222, 239, 272, 276, 417
DpnII GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco105I TACGTA 1 cut(s) 448
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 327
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 477
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 5 cut(s) 146, 212, 349, 481, 500
FaiI YATR 9 cut(s) 107, 109, 144, 210, 334, 336, 347, 479, 498
FaqI GGGAC 1 cut(s) 140
FatI CATG 5 cut(s) 142, 208, 345, 477, 496
FauI CCCGC 1 cut(s) 179
FauNDI CATATG 1 cut(s) 334
FbaI TGATCA 2 cut(s) 205, 220
Fnu4HI GCNGC 4 cut(s) 17, 66, 263, 379
FokI GGATG 2 cut(s) 377, 441
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 4 cut(s) 17, 66, 263, 379
FspBI CTAG 2 cut(s) 308, 510
GluI GCNGC 4 cut(s) 17, 66, 263, 379
HaeIII GGCC 3 cut(s) 82, 162, 171
HapII CCGG 1 cut(s) 159
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 5 cut(s) 146, 212, 349, 481, 500
HinfI GANTC 2 cut(s) 153, 175
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 215
Hpy188I TCNGA 3 cut(s) 225, 232, 415
Hpy188III TCNNGA 2 cut(s) 128, 209
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 2 cut(s) 26, 419
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 2 cut(s) 227, 447
HpyCH4V TGCA 5 cut(s) 102, 341, 349, 390, 472
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 177, 192, 378, 387, 506
HpyF3I CTNAG 1 cut(s) 48
HpySE526I ACGT 2 cut(s) 227, 447
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 5 cut(s) 146, 212, 349, 481, 500
Ksp22I TGATCA 2 cut(s) 205, 220
Kzo9I GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
LmnI GCTCC 2 cut(s) 232, 493
LpnPI CCDG 7 cut(s) 64, 153, 172, 180, 185, 268, 291
Lsp1109I GCAGC 3 cut(s) 28, 77, 249
LweI GCATC 1 cut(s) 496
MaeI CTAG 2 cut(s) 308, 510
MaeII ACGT 2 cut(s) 227, 447
MalI GATC 7 cut(s) 134, 207, 222, 239, 272, 276, 417
MboI GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
MboII GAAGA 3 cut(s) 20, 83, 227
MfeI CAATTG 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 192, 327
MluCI AATT 2 cut(s) 242, 316
MlyI GAGTC 1 cut(s) 162
MmeI TCCRAC 2 cut(s) 210, 336
MnlI CCTC 4 cut(s) 67, 93, 142, 426
MslI CAYNNNNRTG 1 cut(s) 350
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 159, 168
MunI CAATTG 1 cut(s) 316
MvaI CCWGG 1 cut(s) 168
MvnI CGCG 1 cut(s) 195
MwoI GCNNNNNNNGC 6 cut(s) 168, 177, 192, 378, 387, 506
NciI CCSGG 1 cut(s) 159
NcoI CCATGG 1 cut(s) 477
NdeI CATATG 1 cut(s) 334
NdeII GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
NheI GCTAGC 1 cut(s) 509
NlaIII CATG 5 cut(s) 146, 212, 349, 481, 500
NlaIV GGNNCC 4 cut(s) 119, 191, 234, 371
NspI RCATGY 1 cut(s) 500
PaeI GCATGC 1 cut(s) 500
PagI TCATGA 1 cut(s) 208
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 4 cut(s) 18, 67, 264, 380
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Ppu21I YACGTR 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 119, 191, 234, 371
PvuII CAGCTG 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 350
SatI GCNGC 4 cut(s) 17, 66, 263, 379
Sau3AI GATC 7 cut(s) 132, 205, 220, 237, 270, 274, 415
SchI GAGTC 1 cut(s) 162
ScrFI CCNGG 2 cut(s) 159, 168
SduI GDGCHC 2 cut(s) 192, 327
SfaNI GCATC 1 cut(s) 496
SfiI GGCCNNNNNGGCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 350
SmlI CTYRAG 2 cut(s) 128, 326
SmoI CTYRAG 2 cut(s) 128, 326
SnaBI TACGTA 1 cut(s) 448
SphI GCATGC 1 cut(s) 500
Sse9I AATT 2 cut(s) 242, 316
SsiI CCGC 4 cut(s) 186, 193, 286, 379
SspMI CTAG 2 cut(s) 308, 510
StyD4I CCNGG 2 cut(s) 157, 166
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 2 cut(s) 230, 450
TaqI TCGA 1 cut(s) 273
TasI AATT 2 cut(s) 242, 316
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 1 cut(s) 175
TseI GCWGC 3 cut(s) 16, 65, 262
XagI CCTNNNNNAGG 1 cut(s) 423
XapI RAATTY 1 cut(s) 242
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 2 cut(s) 352, 380
XspI CTAG 2 cut(s) 308, 510
ZraI GACGTC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.